A new type of dynamic axial compression chromatography column

Abstract

The utility model discloses a novel dynamic axial compression chromatography column. The chromatography column comprises an external frame, a main driving device is fixedly installed on the top of the external frame, a column is placed in the external frame, a movable piston is placed inside the column, the piston is fixedly connected to the piston rod at the bottom of the main driving device, the lower end of the column is detachably fixedly connected to a bottom end cover, a secondary driving device is arranged below the bottom end cover, the bottom end cover is fixedly connected to the piston rod at the top of the secondary driving device, the secondary driving device is fixedly installed on a movable placement platform, and a plurality of supporting telescopic devices connected to the bottom surface of the bottom end cover are fixedly installed on the placement platform. The upper and lower ends of a novel dynamic axial compression chromatography column in the utility model are respectively provided with main and secondary driving devices, which can reduce the formation of the column driving device, and the secondary driving device can be used as a weight for the column bottom end cover to improve stability.

Description

Technical Field

[0001] The utility model relates to the field of chromatography columns, in particular to a novel dynamic axial compression chromatography column. Background Art

[0002] Liquid chromatography separation technology is a very important technology for the preparation, purification and analysis of proteins, peptides and nucleic acids in the pharmaceutical and biological industries. Among them, in the preparation, purification and analysis of compounds, chromatography separation technology has many applications, mainly for the separation of chemical and biological substances.

[0003] In the prior art, the axial dynamic compression chromatography column used to implement the liquid chromatography separation technology mostly adopts a tubular column, in which the filler is uniformly filled. The filler is generally formed by consolidating a suspension of discrete particles, and the suspension is a homogenate. Usually, the filler is transported from the hole at one end of the column to the column, and then compressed by a plunger so that the filler becomes a uniform packed bed. Therefore, a single cylinder is required to generate thrust, push the plunger downward, enter the pressure-holding state, lift the adapter assembly away from the column tube and lift the column tube away from the lower end. At this time, the formation of a single cylinder often requires 700mm+400mm (according to the common specification of a maximum column bed height of 700mm), so a larger space needs to be reserved for the chromatography column; in addition, the weight of the components of the entire column structure is often too heavy, which is not conducive to the support at the bottom of the chromatography column to play a good stabilizing and load-bearing role. Utility Model Content

[0004] To solve the above problems, the utility model provides a new type of dynamic axial compression chromatography column, which is respectively provided with main and auxiliary drive devices at the upper and lower ends, which can reduce the formation of the column drive device, and the auxiliary drive device can be used as a weight for the bottom end cover of the column to improve stability.

[0005] According to one aspect of the utility model, a novel dynamic axial compression chromatography column is provided, comprising an external frame, a main driving device is fixedly installed on the top of the external frame, a column is placed in the external frame, a movable piston is placed inside the column, the piston is fixedly connected to the piston rod at the bottom of the main driving device, the upper end of the column is also detachably fixedly connected to the lower end of the main driving device, and the lower end of the column is detachably fixedly connected to a bottom end cover; wherein, an auxiliary driving device is arranged below the bottom end cover, and the bottom end cover is fixedly connected to the piston rod at the top of the auxiliary driving device, and the auxiliary driving device is fixedly installed on a movable placement platform, a plurality of supporting telescopic devices are fixedly installed on the placement platform, and each supporting telescopic device is connected to the bottom surface of the bottom end cover.

[0006] In some embodiments, the outer frame is a rectangular structure and is composed of four support columns, and the tops of the support columns are connected to each other. The invention is beneficial in that the specific structure and composition of the outer frame are described.

[0007] In some embodiments, a reinforcing rib is connected between two adjacent support columns. The benefit of this is that the provision of the reinforcing rib can improve the stability of the external frame.

[0008] In some embodiments, the main drive device is a hydraulic cylinder. The benefit of this is that it describes the selection type of the main drive device.

[0009] In some embodiments, the upper end of the column is connected to the lower end of the main drive device through a flange tie rod and fixed by a plurality of nuts. The benefit lies in that a specific method of detachably fixing the upper end of the column to the lower end of the main drive device is described.

[0010] In some embodiments, the lower end of the column is detachably fixedly connected to the bottom end cover in the same manner as the upper end thereof. The benefit of this is that the specific manner in which the lower end of the column is detachably fixedly connected to the bottom end cover is further described.

[0011] In some embodiments, the auxiliary drive device is an oil cylinder driven by a hand pressure pump or an oil cylinder driven by a pneumatic pump. The invention is beneficial in that it describes the selection types of auxiliary drive devices.

[0012] In some embodiments, the piston rod at the top of the auxiliary drive device is fixedly connected to the center of the bottom end cover, which is beneficial in that the stability of the connection between the auxiliary drive device and the bottom end cover can be improved.

[0013] In some embodiments, the placement platform includes a transverse plate, and a plurality of brake universal wheels are arranged below the transverse plate. The benefit lies in that the specific structure of the placement platform is described.

[0014] In some embodiments, the supporting telescopic device includes a sliding sleeve and a sliding rod, the top of the sliding sleeve is fixedly connected to the bottom end cover, the top of the sliding rod extends into the bottom opening of the sliding sleeve, and the bottom is mounted on the movable placement platform. The benefit lies in that the specific structure and connection method of the supporting telescopic device are described. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic structural diagram of a novel dynamic axial compression chromatography column according to one embodiment of the utility model;

[0016] Figure 2 for Figure 1A partial structural schematic diagram of a novel dynamic axial compression chromatography column is shown;

[0017] Figure 3 for Figure 1 A schematic diagram of the top view structure of a new type of dynamic axial compression chromatography column is shown.

[0018] In the figure: external frame 1, main drive device 2, column 3, piston 4, bottom end cover 5, auxiliary drive device 6, movable placement platform 7, support telescopic device 8, support column 9, reinforcing rib 10, flange pull rod 11, nut 12, cross plate 13, brake universal wheel 14, sliding sleeve 15, sliding rod 16. DETAILED DESCRIPTION

[0019] The utility model is further described in detail below in conjunction with the accompanying drawings.

[0020] like Figure 1-3 As shown, the chromatography column comprises an outer frame 1, wherein the outer frame 1 is a rectangular structure and is composed of four support columns 9, and the tops of the support columns 9 are connected to each other. In order to improve stability, reinforcing ribs 10 may be provided between two adjacent support columns 9.

[0021] A main drive device 2 is fixedly installed on the top of the external frame 1. The main drive device 2 adopts a hydraulic cylinder. A column 3 is placed in the external frame 1. A movable piston 4 is placed inside the column 3. The piston 4 is fixedly connected to a piston rod extending from the bottom of the main drive device 2. The main drive device 2 can drive the piston 4 to move inside the column 3.

[0022] The upper end of the column 3 is also connected to the lower end of the main drive device 2 through a flange tie rod 11, and is compressed and fixed by a plurality of nuts 12, thereby realizing a detachable fixed connection between the column 3 and the main drive device 2. At the same time, the lower end of the column 3 is also detachably fixedly connected to a bottom end cover 5 in the same manner.

[0023] An auxiliary driving device 6 is arranged below the bottom end cover 5, and the auxiliary driving device 6 adopts a cylinder driven by a hand pressure pump or a cylinder driven by a pneumatic pump. The bottom end cover 5 is fixedly connected to the piston rod extending from the top of the auxiliary driving device 6, and in order to improve stability, the piston rod of the auxiliary driving device 6 can be fixedly connected to the center of the bottom end cover 5.

[0024] The auxiliary driving device 6 is fixedly mounted on a movable placement platform 7. The movable placement platform 7 has a transverse plate 13, on which the auxiliary driving device 6 is mounted, and below the transverse plate 13, a plurality of brake universal wheels 14 are arranged.

[0025] A plurality of supporting and telescopic devices 8 are fixedly mounted on the movable placement platform 7, and each supporting and telescopic device 8 is connected to the bottom surface of the bottom end cover 5. Each supporting and telescopic device 8 includes a sliding sleeve 15 and a sliding rod 16. The sliding sleeve 15 opens downward and its top is fixedly connected to the bottom end cover 5. The top of the sliding rod 16 extends into the bottom opening of the sliding sleeve 15, and the bottom of the sliding rod 16 is mounted on the movable placement platform 7. Thus, through the support of the supporting and telescopic devices 8, the bottom end cover 5 and the entire column 3 can be stably moved up and down.

[0026] When using the chromatography column, first install the outer frame 1 and the main drive device 2, and install the bottom of the column 3 and the bottom end cover 5. In this way, the column 3 can be moved through the movable placement platform 7 for easy transportation. Then move the piston rod of the main drive device 2 downward, and then fix the piston. After the installation is completed, move the piston rod of the main drive device 2 upward to push the column 3 into the outer frame 1 and place it below the piston 4.

[0027] The auxiliary drive device 6 starts to work, and under its action, the column 3 and the bottom end cover 5 move upward, allowing the piston 4 to slowly enter the column 3 until the cross-section of the piston 4 is parallel to the upper end of the column 3, and the upper end of the column 3 is connected to the lower end of the main drive device 2 through the flange tie rod 11, and then tightened and fixed with nuts 12.

[0028] The homogenate enters the column 3 through the inlet on the piston 4. When a certain amount is reached, the injection of the homogenate is stopped. At this time, the piston 4 moves downward under the action of the hydraulic cylinder, the mobile phase port at the upper end of the column 3 opens, and the mobile phase port at the lower end closes, which is convenient for exhausting the air in the column 3, and switches to the lower open and upper closed glue pressing after the exhaust is completed. After the piston 4 moves downward to reach the set glue pressing pressure, it enters the pressure holding state.

[0029] In the normal use process steps of the chromatography column, the glue needs to be unloaded after the process is completed. At this time, the nut 12 of the lower bottom cover 5 is loosened, and the auxiliary driving device 6 is started to move downward to make the bottom cover 5 separate from the column 3. The bottom cover 5 is then removed through the movable placement platform 7 and pushed into the filler recovery container; then, the main driving device 2 is started to make the piston 4 continue to press downward, so that the filler can be pressed out of the column 3 and into the recovery container until the piston 4 reaches the lowest position.

[0030] The above are only some embodiments of the present invention. For those skilled in the art, several modifications and improvements can be made without departing from the inventive concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A novel dynamic axial compression chromatography column, characterized in that: The invention comprises an external frame (1), a main drive device (2) is fixedly mounted on the top of the external frame (1), a column (3) is placed in the external frame (1), a movable piston (4) is placed inside the column (3), the piston (4) is fixedly connected to the piston rod at the bottom of the main drive device (2), the upper end of the column (3) is also detachably fixedly connected to the lower end of the main drive device (2), and the lower end of the column (3) is detachably fixedly connected to a bottom end cover (5); wherein an auxiliary drive device (6) is arranged below the bottom end cover (5), and the bottom end cover (5) is fixedly connected to the piston rod at the top of the auxiliary drive device (6), and the auxiliary drive device (6) is fixedly mounted on a movable placement platform (7), and a plurality of supporting telescopic devices (8) are fixedly mounted on the placement platform, and each supporting telescopic device (8) is connected to the bottom surface of the bottom end cover (5).

2. A novel dynamic axial compression chromatography column according to claim 1, characterized in that: The external frame (1) is of a rectangular structure and is composed of four support columns (9), and the tops of the support columns (9) are connected to each other.

3. A novel dynamic axial compression chromatography column according to claim 2, characterized in that: A reinforcing rib (10) is connected between two adjacent support columns (9).

4. A novel dynamic axial compression chromatography column according to claim 1, characterized in that: The main driving device (2) adopts a hydraulic cylinder.

5. A novel dynamic axial compression chromatography column according to claim 1, characterized in that: The upper end of the column (3) is connected to the lower end of the main drive device (2) via a flange tie rod (11) and is fixed via a plurality of nuts (12).

6. A novel dynamic axial compression chromatography column according to claim 5, characterized in that: The lower end of the column (3) is detachably fixedly connected to the bottom end cover (5) in the same manner as the upper end.

7. A novel dynamic axial compression chromatography column according to claim 1, characterized in that: The auxiliary driving device (6) is an oil cylinder driven by a hand pressure pump or an oil cylinder driven by a pneumatic pump.

8. A novel dynamic axial compression chromatography column according to claim 1, characterized in that: The piston rod at the top of the auxiliary driving device (6) is fixedly connected to the center of the bottom end cover (5).

9. A novel dynamic axial compression chromatography column according to claim 1, characterized in that: The placement platform (7) comprises a transverse plate (13), and a plurality of brake universal wheels (14) are arranged below the transverse plate (13).

10. A novel dynamic axial compression chromatography column according to claim 1, characterized in that: The supporting telescopic device (8) comprises a sliding sleeve (15) and a sliding rod (16), wherein the top of the sliding sleeve (15) is fixedly connected to the bottom end cover (5), the top of the sliding rod (16) extends into the bottom opening of the sliding sleeve (15), and the bottom is mounted on the movable placement platform (7).

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