A multifunctional operating table for Elisa experiments

Abstract

A multifunctional operating table for Elisa experiments comprises a shell, a plurality of supporting legs are arranged at the bottom of the shell, a pair of brackets are arranged in the shell, a supporting plate is arranged between the pair of brackets, a heating shell for storing heating liquid is arranged in the shell, a heater and a temperature sensor are arranged in the heating shell, and a plurality of sliders that slide with the supporting plate are fixedly arranged at the bottom of the heating shell; a driving motor is also arranged in the shell, and the driving motor drives the heating shell to reciprocate through a transmission mechanism; an ELISA plate is arranged on the upper seat of the heating shell, and a 96-well rack corresponding to the ELISA plate is arranged on the shell; a vertical plate is arranged on the top of the shell, a guide rod is arranged on the vertical plate, and a row gun pipette is slidably arranged on the guide rod; the heater, the temperature sensor and the driving motor are all electrically connected to a controller arranged on the shell. The multifunctional operating table for Elisa experiments provided by the utility model has a structural design that not only facilitates the experimenter to accurately control the experimental operation process, but also ensures the experimental effect, and the structural design is ingenious and highly practical.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental operation equipment, in particular to a multifunctional operating table for Elisa experiments. Background Art

[0002] Enzyme-Linked Immunosorbent Assay (ELISA) is an analytical technique widely used in biology, medicine, and biotechnology. Its basic principle is based on the specific binding between antigen and antibody, and the detection of this binding by enzyme labeling technology. The qualitative or quantitative analysis of antigen or antibody is achieved by enzyme-catalyzed substrate color development. Due to its high sensitivity and specificity, it is one of the important experiments used in laboratories to detect cytokines, protein expression and purity. In the ELISA experiment, multiple experimental steps are involved: 1. Prepare all reagents and samples to be tested in advance. 2. Add standard or sample to be tested, incubate at 37°C for 80 minutes. 3. Wash the plate 3 times and pat dry. 4. Add biotin antibody working solution to each well and incubate at 37°C for 50 minutes. 5. Wash the plate 3 times and pat dry. 6. Add enzyme conjugate working solution to each well and incubate at 37°C for 50 minutes. 7. Discard the liquid in the well, wash the plate 5 times and pat dry. 8. Add color substrate solution to each well and incubate at 37°C in the dark for 20 minutes. 9. Add stop solution to each well. 10. Immediately measure the optical density (OD value) of each well using an ELISA reader.

[0003] There are some disadvantages in the operation of this experiment, such as: 1. Since reactants need to be added and the plate needs to be washed many times, the discharge gun needs to be used many times. When using the discharge gun, it is inevitable that mistakes may not be made to add the reagents into the wells correctly. In step 9, the color developing solution needs to be added, so the experimental operator needs to add the sample in a dark room, and it is even more impossible to avoid errors in the operation of the discharge gun. 2. Since multiple incubations are required and the required temperatures are different, the temperature of the incubation machine needs to be set repeatedly in advance, and there is no timing function; 3. After adding the color developing solution in step 9, it needs to be protected from light, but the ELISA plate does not have this function.

[0004] Therefore, a new operating device is needed to solve the above technical problems. Utility Model Content

[0005] The purpose of the utility model is to provide a multifunctional operating table for Elisa experiments in view of the shortcomings of the prior art. The specific scheme is as follows:

[0006] A multifunctional operating table for Elisa experiments comprises an outer shell, a plurality of supporting legs are arranged at the bottom of the outer shell, a pair of brackets are arranged in the outer shell, a supporting plate is arranged between the pair of brackets, a heating shell for storing heating liquid is arranged in the outer shell, a heater and a temperature sensor are arranged in the heating shell, and a plurality of sliding blocks slidably matched with the supporting plate are fixedly arranged at the bottom of the heating shell; a driving motor is also arranged in the outer shell, and the driving motor drives the heating shell to reciprocate through a transmission mechanism; an ELISA plate is arranged on the upper seat of the heating shell, and a 96-well rack corresponding to the ELISA plate is arranged on the outer shell; a vertical plate is arranged on the top of the outer shell, a guide rod is arranged on the vertical plate, and a pistol pipette is slidably arranged on the guide rod; the heater, the temperature sensor and the driving motor are all electrically connected to a controller arranged on the outer shell.

[0007] Based on the above, a support block is provided on the shell, a shading plate for shielding the 96-hole rack is placed on the support block, a limit frame for limiting the moving direction of the shading plate is also provided on the shell, and a handle is also provided on the shading plate.

[0008] Based on the above, a proximity switch for the row gun pipette is provided at the end of each column of holes on the 96-hole rack, and a corresponding indicator light is provided for each proximity switch on the 96-hole rack. The proximity switches and indicator lights are electrically connected to the controller.

[0009] Based on the above, the transmission mechanism includes a turntable fixedly mounted at the power output end of the driving motor, the turntable is connected to one end of connecting rod one through pin shaft one, the other end of connecting rod one is connected to one end of connecting rod two through pin shaft two, the other end of connecting rod two is connected to a connecting head fixed on the heating shell through pin shaft three, and a guide frame is also provided in the outer shell, and connecting rod two slides through the guide frame.

[0010] The utility model has substantial characteristics and progress compared with the prior art. Specifically, the utility model has the following advantages:

[0011] The multifunctional operating table for Elisa experiments provided by the utility model has a structural design that not only facilitates the experimenter to accurately control the experimental operation process, but also can ensure the experimental effect, and has the technical advantages of ingenious structural design and strong practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0013] Figure 2 It is the front view of the utility model.

[0014] Figure 3 It is a schematic diagram of the partial matching structure of the utility model.

[0015] In the figure: 1. Shell; 1-1. Operating door; 1-2. Limit plate; 1-3. Handle; 2. Heating shell; 3. Bracket; 4. Support plate; 5. Slider; 6. Heater; 7. Temperature sensor; 8. ELISA plate; 9. Driving motor; 10. Turntable; 11. Connecting rod one; 12. Connecting rod two; 13. Guide frame; 14. 96-hole frame; 15. Vertical plate; 16. Guide rod; 17. Drain gun pipette; 18. Proximity switch; 19. Indicator light; 20. Support block; 21. Shading plate; 22. Limit frame; 23. Handle; 24. Controller. DETAILED DESCRIPTION

[0016] The technical solution of the utility model is further described in detail below through specific implementation methods. Example

[0017] like Figure 1-3 As shown, the utility model provides a multifunctional operating table for Elisa experiments, including a shell 1, a plurality of supporting legs are provided at the bottom of the shell 1, a pair of brackets 3 are provided in the shell 1, a support plate 4 is provided between the pair of brackets 3, a heating shell 2 for storing heating liquid is provided in the shell 1, a heater 6 and a temperature sensor 7 are provided in the heating shell 2, and a plurality of sliders 5 slidably matched with the support plate 4 are fixedly provided at the bottom of the heating shell 2; a driving motor 9 is also provided in the shell 1, and the driving motor 9 drives the heating shell 2 to reciprocate through a transmission mechanism; an ELISA plate 8 is provided on the upper seat of the heating shell 2, and a 96-well rack 14 corresponding to the ELISA plate 8 is provided on the shell 1; a vertical plate 15 is provided on the top of the shell 1, a guide rod 16 is provided on the vertical plate 15, and a discharge gun pipette 17 is slidably provided on the guide rod 16; the heater 6, the temperature sensor 7 and the driving motor 9 are all electrically connected to a controller 24 arranged on the shell 1.

[0018] Taking into account that some experimental processes need to be carried out in the dark, a support block 20 is provided on the outer shell 1, and a shading plate 21 for shielding the 96-hole rack 14 is placed on the support block 20. A limit frame 23 for limiting the moving direction of the shading plate 21 is also provided on the outer shell 1, and a handle 23 is also provided on the shading plate 21.

[0019] During the experiment, sometimes it is necessary to use a pistol discharge pipette 17 to add reagents to the ELISA plate 8 through the 96-well rack 14 in a darker environment. To ensure that the experimental operation is carried out accurately, a proximity switch 18 for the pistol discharge pipette 17 is provided at the end of each column of holes on the 96-well rack 14, and a corresponding indicator light 19 is provided on the 96-well rack 14 corresponding to each of the proximity switches 18. The proximity switches 18 and indicator lights 19 are electrically connected to the controller 24. When the pistol discharge pipette 17 is used to add reagents to the ELISA plate 8 through the 96-well rack 14, whenever the pistol discharge pipette 17 moves to a position close to a proximity switch 18, the indicator light 19 corresponding to the proximity switch 18 lights up, and the experimenter can accurately add reagents to the ELISA plate 8 through the pistol discharge pipette 17. It should be noted that each time the reagent is added, the shading plate 21 needs to be moved to cover the through holes on the corresponding 96-well rack 14 to ensure the experimental effect.

[0020] The transmission mechanism includes a turntable 10 fixedly mounted on the power output end of the driving motor 9, the turntable 10 is connected to one end of the connecting rod 11 through a pin shaft 1, the other end of the connecting rod 11 is connected to one end of the connecting rod 2 12 through a pin shaft 2, the other end of the connecting rod 2 12 is connected to a connector fixed on the heating shell 2 through a pin shaft 3, and a guide frame 13 is also provided in the housing 1, and the connecting rod 2 12 slides through the guide frame 13. When the driving motor 9 rotates, the heating shell 2 can be driven to shake left and right through the transmission mechanism, thereby facilitating the experiment.

[0021] It should be noted that in order to facilitate experimental operations on the ELISA plate 8 and inspection and maintenance of the equipment inside the shell 1, an operating door 1-1 is hingedly provided on the shell 1, and a limit plate 1-2 is provided on the operating door 1-1. The limit plate 1-2 and the shell 1 are provided with corresponding magnets, and the operating door 1-1 is also provided with a handle 1-3.

[0022] In addition, considering that the experimental process sometimes requires light shielding, the above-mentioned shell 1 and the operating door 1-1 are made of opaque material.

[0023] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the utility model can still be modified or some technical features can be replaced by equivalents without departing from the spirit of the technical solution of the utility model, which should be included in the scope of the technical solution for protection of the utility model.

Claims

1. A multifunctional operating table for Elisa experiment, characterized by: The invention comprises a shell (1), wherein a plurality of supporting legs are provided at the bottom of the shell (1), a pair of brackets (3) are provided inside the shell (1), a supporting plate (4) is provided between the pair of brackets (3), a heating shell (2) for storing heating liquid is provided inside the shell (1), a heater (6) and a temperature sensor (7) are provided inside the heating shell (2), and a plurality of sliding blocks (5) slidably matched with the supporting plate (4) are fixedly provided at the bottom of the heating shell (2); a driving motor (9) is also provided inside the shell (1), and the driving motor (9) is driven by The transmission mechanism drives the heating shell (2) to reciprocate; an ELISA plate (8) is provided on the upper seat of the heating shell (2), and a 96-well rack (14) corresponding to the ELISA plate (8) is provided on the outer shell (1); a vertical plate (15) is provided on the top of the outer shell (1), a guide rod (16) is provided on the vertical plate (15), and a discharge gun pipette (17) is slidably provided on the guide rod (16); the heater (6), the temperature sensor (7) and the drive motor (9) are all electrically connected to a controller (24) provided on the outer shell (1).

2. The multifunctional operating table for Elisa experiment according to claim 1, characterized in that: A support block (20) is provided on the housing (1), a light shielding plate (21) for shielding the 96-hole rack (14) is placed on the support block (20), a limiting frame (22) for limiting the moving direction of the light shielding plate (21) is also provided on the housing (1), and a handle (23) is also provided on the light shielding plate (21).

3. The multifunctional operating table for Elisa experiment according to claim 2, characterized in that: A proximity switch (18) for the row gun pipette (17) is provided at the end of each row of holes on the 96-hole rack (14), and a corresponding indicator light (19) is provided for each proximity switch (18) on the 96-hole rack (14). The proximity switches (18) and indicator lights (19) are electrically connected to the controller (24).

4. The multifunctional operating table for Elisa experiment according to claim 1, characterized in that: The transmission mechanism comprises a turntable (10) fixedly mounted on the power output end of the drive motor (9); the turntable (10) is connected to one end of a connecting rod (11) via a pin shaft 1; the other end of the connecting rod (11) is connected to one end of a connecting rod (12) via a pin shaft 2; the other end of the connecting rod (12) is connected to a connecting head fixedly mounted on the heating shell (2) via a pin shaft 3; a guide frame (13) is further provided in the shell (1); the connecting rod (12) slides through the guide frame (13).

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