An organoid slice pressing device

Abstract

The present application discloses an organoid slice pressing device, which relates to the field of bioengineering technology. The device solves the problem of high difficulty in operating small samples and easy information loss during slicing. The organoid slice pressing device includes a support frame and a lifting platform arranged in the support frame; a workbench is provided on the upper surface of the lifting platform; a placement slot is provided on the workbench; the placement slot is used to place a sample slide with an organoid test sample; the upper surface of the support frame is used to place a pressing slide; the pressing slide is located above the sample slide, and a counterweight is provided on the upper surface of the pressing slide.

Description

Technical Field

[0001] The present application relates to the field of bioengineering technology, and in particular to an organoid slice pressing device. Background Art

[0002] Driven by precision medicine, personalized treatment has gradually become the trend of precision treatment. Organoid technology, as an in vitro 3D culture technology, has gradually become a common means of personalized medicine, but the expression of cells cultured in vitro is often different from that in vivo. For example, the main expression method of organoid cells is to perform immunofluorescence staining on the sample after slicing and single-cell transcriptomics analysis on the entire organoid. However, for primary engineered organoid samples, due to their small size and small number of cells, it is difficult to retain complete organoid information after slicing, so it is difficult to perform immunofluorescence staining or spatial transcriptome analysis on engineered organoids. Based on this, this application pioneered the idea of ​​​​flattening organoids into a single cell layer. Utility Model Content

[0003] The embodiments of the present application provide an organoid slice pressing device, which solves the problems of high difficulty in operating small samples and easy information loss in traditional tissue slicing methods such as frozen sections or paraffin sections.

[0004] To achieve the above-mentioned purpose, an embodiment of the present application provides an organoid sheet pressing device, comprising a supporting frame and a lifting platform arranged in the supporting frame; a workbench is provided on the upper surface of the lifting platform; a placement slot is opened on the workbench; the placement slot is used to place a sample slide with an organoid test sample; the upper surface of the supporting frame is used to place a pressing slide; the pressing slide is located above the sample slide, and a counterweight is provided on the upper surface of the pressing slide.

[0005] Furthermore, the placement groove is a rectangular groove with one end penetrating therethrough, and the width of the placement groove is adapted to the width of the sample slide; a rectangular through hole for taking and placing the sample slide is provided at the bottom of the placement groove.

[0006] Furthermore, the workbench is a rectangular plate; the supporting frame includes an upper rectangular frame; the length directions of the upper rectangular frame and the placement groove are consistent with the length direction of the workbench, and the placement groove is located at the center of the workbench; grooves are provided in the centers of the two long sides of the workbench; the centers of the two long sides of the upper rectangular frame extend inwardly to form supporting protrusions; the supporting protrusions are adapted to the size of the grooves.

[0007] Furthermore, the counterweight is a weight.

[0008] Furthermore, a hydrophobic film is provided on the sample slide.

[0009] Furthermore, the workbench is manufactured by machining, casting or 3D printing.

[0010] Furthermore, the support frame also includes a lower rectangular bottom plate and four support rods; the support rods are connected to the four corners of the lower rectangular bottom plate and the upper rectangular frame.

[0011] Furthermore, the lower rectangular bottom plate and the upper rectangular frame are both transparent plates.

[0012] Furthermore, the workbench is connected to the lifting platform by bolts.

[0013] Furthermore, the lifting platform is connected to the lower rectangular bottom plate of the supporting frame by bolts.

[0014] Compared with the prior art, this application has the following beneficial effects:

[0015] 1. The organoid slice pressing device of the embodiment of the present application is provided with a lifting platform in a supporting frame, a working table is provided on the lifting platform, and a placement slot for placing a sample slide is opened on the work table, a pressing slide is placed on the upper surface of the supporting frame, a counterweight is placed on the pressing slide, and the pressing slide is located above the sample slide, and the lifting platform is driven to drive the work table to move upward so that the sample slide contacts the pressing slide to achieve pressing of the sample, thereby, there is no need to slice the organoid sample, and the in situ RNA information of the cells can be retained after the organoid is pressed, which solves the problem of high difficulty in operating small samples and easy loss of information when slicing the samples.

[0016] 2. The organoid sheet pressing device of the embodiment of the present application achieves standardization and repeatability of the pressing process by using a lifting platform with uniform and controllable pressure during the pressing process.

[0017] 3. The organoid sheet pressing device of the embodiment of the present application can achieve the pressing of various primary organoids by changing the pressing pressure and pressing method, and has a wider range of applications.

[0018] 4. The organoid slice pressing device of the embodiment of the present application can be adapted to the BGI stereo-seq spatial transcriptome chip.

[0019] 5. The organoid slice pressing device of the embodiment of the present application reduces the shaking of the device during operation by placing weights on the pressing glass slide, thereby transferring contact pressure. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 This is a schematic diagram of the three-dimensional structure of the organoid slice pressing device according to an embodiment of the present application;

[0022] Figure 2 This is a schematic diagram of the three-dimensional structure of the organoid slice pressing device of the embodiment of the present application after the sample slide is installed and the slide is pressed;

[0023] Figure 3 This is an ss-DNA fluorescent staining image of a mouse lung organoid after being pressed using the organoid slice pressing device of an embodiment of the present application;

[0024] Figure 4 This is an image of ss-DNA fluorescent staining of mouse liver organoids after being pressed using the organoid slice pressing device of an embodiment of the present application. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0026] In the description of the present application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0027] In the description of the present application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0028] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, "plurality" means two or more.

[0029] Reference Figure 1 and Figure 2 An embodiment of the present application provides an organoid sheet pressing device, comprising a supporting frame 1, a lifting platform 2, a workbench 3 and a counterweight 4.

[0030] The support frame 1 includes an upper rectangular frame 11, a lower rectangular bottom plate 12 and four support rods 13. The lower rectangular bottom plate 12 and the upper rectangular frame 11 are both transparent plates. The support rods 13 are connected to the four corners of the lower rectangular bottom plate 12 and the upper rectangular frame 11. The centers of the two long sides of the upper rectangular frame 11 extend inward to form support protrusions 14, and the upper surface of the support protrusions 14 is used to place the pressing slide 5, and the size of the support protrusions 14 is adapted to the size of the pressing slide 5. Thus, it is convenient to position the pressing slide 5.

[0031] The lifting platform 2 is connected to the lower rectangular bottom plate 12 of the support frame 1 by bolts and is located inside the support frame 1. The upper surface of the lifting platform 2 is connected to the workbench 3 by bolts. The workbench 3 is a rectangular plate made of R4600 resin. The length direction of the workbench 3 is consistent with the length direction of the upper rectangular frame 11. The centers of the two long sides of the workbench 3 are provided with grooves 31. The position of the grooves 31 corresponds to the position of the support protrusions 14, and the size of the grooves 31 is adapted to the size of the support protrusions 14.

[0032] A placement groove 32 for placing a sample slide 6 with an organoid test sample is provided in the center of the workbench 3. The placement groove 32 is a rectangular groove, and the length direction of the placement groove 32 is consistent with the length direction of the workbench 3. In this way, before pressing, the pressing slide 5 is located above the sample slide 6, and the pressing slide 5 coincides with the center of the sample slide 6 and is perpendicular to each other, forming a "cross" shape. As a result, it is not easy to be misplaced when pressing. In addition, the width of the placement groove 32 is adapted to the width of the sample slide 6. In order to facilitate the placement of the sample slide 6, one end of the placement groove 32 passes through the short side of the workbench 3, and a rectangular through hole 33 for placing the sample slide 6 is also provided at the bottom of the placement groove 32.

[0033] Since the pressing slide 5 is light and not fixed on the upper rectangular frame 11, its own gravity cannot reach the required pressing force. Therefore, the embodiment of the present application places a counterweight 4 on the pressing slide 5 to counterweight it. Specifically, the counterweight 4 can be one or more weights. Since the weights are standard parts, the accuracy is higher and the cost is lower. In addition, the pressing force can be adjusted by replacing weights of different specifications, so that the scope of use of the embodiment of the present application is wider. It should be noted that when placing multiple weights, weights of different weights need to be evenly distributed on the pressing slide 5.

[0034] The sample slide 6 is covered with a hydrophobic film. Specifically, the hydrophobic film can be a PTFE film, thereby ensuring that after the pressing is completed, the cells in the sample will not adhere to the pressing slide above.

[0035] The use process of the organoid slice pressing device in the embodiment of the present application is as follows:

[0036] Before pressing, cover the sample surface with a hydrophobic film, then install the sample slide 6 in the placement groove 32, and drive the lifting platform 2 to the lowest point. Then, place the pressing slide 5 on the upper surface of the supporting protrusion 14. At this time, the pressing slide 5 and the sample are not in contact and are in a separated state. After the placement is completed, place a weight on the upper surface of the pressing slide 5, and drive the lifting platform 2 to rise until the sample slide 6 contacts the pressing slide 5, and the pressing slide 5 is separated from the supporting protrusion. After waiting for 5 seconds, remove the weight vertically, then remove the pressing slide 5, and use tweezers to clamp the hydrophobic film from one end to separate it, and the final sample can be obtained.

[0037] Reference Figure 3 and Figure 4 It can be seen that the green fluorescence represents the staining result of the ss-DNA signal, which can be located in the cell nucleus. From the results of fluorescence staining, it can be seen that the pressing effect of the device on different organoids can reach the level of a single cell layer, and there will be no cell stacking phenomenon.

[0038] The above are only specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. An organoid sheet pressing device, characterized in that: It comprises a support frame and a lifting platform arranged in the support frame; a workbench is arranged on the upper surface of the lifting platform; a placement slot is provided on the workbench; the placement slot is used to place a sample slide with an organoid test sample; the upper surface of the support frame is used to place a pressing slide; the pressing slide is located above the sample slide, and a counterweight is provided on the upper surface of the pressing slide.

2. The organoid sheet pressing device according to claim 1, characterized in that: The placement groove is a rectangular groove with one end penetrating therethrough, and the width of the placement groove is adapted to the width of the sample slide; a rectangular through hole for taking and placing the sample slide is provided at the bottom of the placement groove.

3. The organoid sheet pressing device according to claim 2, characterized in that: The workbench is a rectangular plate; the supporting frame includes an upper rectangular frame; the length directions of the upper rectangular frame and the placement groove are consistent with the length direction of the workbench, and the placement groove is located at the center of the workbench; grooves are provided in the centers of the two long sides of the workbench; the centers of the two long sides of the upper rectangular frame extend inwardly to form supporting protrusions; the supporting protrusions are adapted to the size of the grooves.

4. The organoid sheet pressing device according to claim 3, characterized in that: The counterweight is a weight.

5. The organoid sheet pressing device according to claim 4, characterized in that: The sample glass slide is provided with a hydrophobic film.

6. The organoid sheet pressing device according to claim 5, characterized in that: The workbench is manufactured by machining, casting or 3D printing.

7. The organoid sheet pressing device according to claim 6, characterized in that: The support frame also includes a lower rectangular bottom plate and four support rods; the support rods are connected to the four corners of the lower rectangular bottom plate and the upper rectangular frame.

8. The organoid sheet pressing device according to claim 7, characterized in that: The lower rectangular bottom plate and the upper rectangular frame are both transparent plates.

9. The organoid sheet pressing device according to claim 8, characterized in that: The workbench is connected to the lifting platform by bolts.

10. The organoid sheet pressing device according to claim 9, characterized in that: The lifting platform is connected to the rectangular bottom plate at the lower end of the supporting frame by bolts.

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