Dry powder culture medium integrated storage and quick preparation device

Abstract

The utility model discloses a dry powder culture medium integrated storage and quick preparation device which comprises a charging bag body, a culture bottle connected with the charging bag body, a telescopic cutting mechanism, a dry powder bin, a storage bin, a bottle cap, a tool bit control device and a cutting tool bit. The dry powder culture medium preparation device has the beneficial effects that each component required by the preparation of a dry powder culture medium is separately stored, the operation is convenient during preparation, the integrated preparation of the dry powder culture medium is realized, and the filtering sterilization is not needed.

Description

Technical Field

[0001] The utility model relates to a culture medium device, in particular to an integrated storage and quick-matching device for dry powder culture medium. Background Art

[0002] There are many types of cell culture media, which are divided into synthetic culture media and natural culture media according to their sources (most of the culture media currently used are synthetic culture media), and divided into dry powder culture media and liquid culture media according to their material state. Dry powder culture media need to be prepared and sterilized by the experimenter himself, with a low purchase cost but a more complicated preparation process; liquid culture media are provided by professional merchants and can be used directly by users, which is very convenient, but the purchase cost is high. There is also a big difference in the shelf life of liquid culture media and dry powder culture media. When stored in a light-proof environment at 2 to 8 ° C, the shelf life of liquid culture media ranges from 3 to 12 months, while the shelf life of dry powder culture media can reach 2 to 3 years. After the existing dry powder culture media are prepared into liquid culture media, they need to be sterilized and filtered, and the preparation process is relatively complicated. The amount of culture medium used in small-volume cell culture and high-throughput screening experiments is small, and the power consumption per unit volume of weighing materials by yourself or using dry powder to prepare small-volume culture media is large. The price of commercially available liquid culture media is generally high, the shelf life is short, it cannot be stored for a long time, and the economic cost is high. Utility Model Content

[0003] In view of the deficiencies in the prior art, the technical problem to be solved by the utility model is to provide an integrated storage and quick-mixing device for dry powder culture medium which is easy to operate and can be used directly after preparation without filtering and sterilization.

[0004] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0005] A dry powder culture medium integrated storage and quick-matching device comprises a charging bag body and a culture bottle connected to the charging bag body, characterized in that: the charging bag body is provided with a virtual welding line to divide its interior into a dry powder bin and a storage cavity, the virtual welding line can be opened under the action of external force to make the storage cavity communicate with the dry powder bin, the dry powder bin and the storage cavity are respectively provided with a sealable feeding port, the side wall of the dry powder bin is provided with a telescopic cutting mechanism, the outer wall of the telescopic cutting mechanism is provided with a thread, the bottle cap is connected to the telescopic cutting mechanism through the thread, the bottle cap is located outside the dry powder bin, the inner wall of the telescopic cutting mechanism is provided with a cutter head control device and a cutting cutter head, the bottom of the charging bag body is provided with a threaded connection mechanism matching the bottle mouth of the culture bottle, the cutter head control device is used to control the movement of the cutting cutter head, and under the action of external force, the cutting cutter head can destroy the bottom surface of the charging bag body at the bottle mouth, thereby making the dry powder bin communicate with the culture bottle.

[0006] Preferably, the filling bag body and the culture bottle are connected via a threaded connection mechanism.

[0007] Preferably, the bottle cap matches the bottle mouth, and after the preparation is completed, the bottle cap can be screwed on the bottle mouth to seal and preserve the culture medium.

[0008] Preferably, the bottle cap matches the bottle mouth, and after the preparation is completed, the bottle cap can be screwed on the bottle mouth to seal and preserve the culture medium.

[0009] Preferably, the cutter head control device is a retractable hose, and in the retracted state the cutting cutter head is located inside the hose to prevent it from cutting through the bag body when not in use. The hose can be extended under the action of an external force so that the cutting cutter head extends out of the hose to cut through the bottom surface of the bag body.

[0010] Preferably, the cutter head control device is arranged outside the dry powder bin.

[0011] Preferably, the cutting blade is made of non-conical tetrafluoroplastic, so that after cutting the bottom surface of the bag body, the bag remains connected to the culture bottle and does not fall off.

[0012] Preferably, the material of the bag body of the filling bag is selected from polyvinyl chloride, polyethylene, polyethylene terephthalate, ethylene-vinyl acetate copolymer or polypropylene.

[0013] Preferably, the bag body of the charging bag is a structure formed by a double-layer base film layer, and the telescopic cutting mechanism is arranged on one of the base film layers. Before loading, the double-layer base film layer is sealed by a heat seal line around the bag body except for the position of the feed port. After loading, the feed port of the bag body is sealed by heat sealing welding, so that the bag body of the charging bag becomes a closed structure.

[0014] Preferably, the virtual weld line can be opened by squeezing the dry powder bin, which increases the pressure inside the dry powder bin and squeezes the virtual weld line open, so that the storage cavity and the dry powder bin are connected; or the virtual weld line can be opened by pulling the bag body of the loading bag near it to both sides, so that the storage cavity and the dry powder bin are connected.

[0015] Compared with the prior art, the utility model has the following beneficial effects: the utility model provides an integrated storage and quick-mixing device for dry powder culture medium, which can be sterilized as a whole after packaging, is easy to operate during preparation, can be used directly after preparation without filtering and sterilization, and has a wide capacity range, reducing economic costs. It solves the problem of complicated preparation process of dry powder culture medium currently used, and reduces the cost of use. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0017] Figure 2 It is a schematic diagram of the front structure of the utility model.

[0018] Figure 3 It is a partially enlarged schematic diagram of the side structure and telescopic cutting mechanism of the utility model.

[0019] In the figure: 1-bag body; 2-heat sealing line; 3-telescopic cutting mechanism; 4-feeding port; 6-dry powder bin; 7-storage cavity; 8-bottle mouth; 9-culture bottle; 10-threaded connection mechanism; 11-dummy welding line; 12-bottom surface of bag body; 31-bottle cap; 32-cutter head control device; 33-cutting knife head. DETAILED DESCRIPTION

[0020] The present invention is further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0021] Example 1

[0022] like Figure 1 , Figure 2 and Figure 3 As shown, the utility model is an integrated storage and quick-mixing device for dry powder culture medium, comprising a charging bag body 1, a culture bottle 9 connected to the charging bag body 1, the charging bag body 1 is provided with a virtual welding line 11 to divide its interior into a dry powder bin 6 and a storage cavity 7, the virtual welding line 11 can be opened under the action of an external force to make the storage cavity 7 communicate with the dry powder bin 6, the dry powder bin 6 and the storage cavity 7 are respectively provided with a sealable feeding port 4, the side wall of the dry powder bin 6 is provided with a telescopic cutting mechanism 3, the outer wall of the telescopic cutting mechanism 3 is provided with a thread, and the bottle The cap 31 is connected to the telescopic cutting mechanism 3 through a thread, the bottle cap 31 is located outside the dry powder bin 6, a cutter head control device 32 and a cutting cutter head 33 are arranged inside the telescopic cutting mechanism 3, a threaded connection mechanism 10 matching the bottle mouth 8 of the culture bottle is arranged at the bottom of the bag body 1 of the loading bag, the cutter head control device 32 is used to control the movement of the cutting cutter head 33, and under the action of an external force, the cutting cutter head 33 can destroy the bottom surface 12 of the bag body of the loading bag at the bottle mouth 8, thereby connecting the dry powder bin 6 with the culture bottle 9. The bag body 1 of the loading bag and the culture bottle 9 are connected through a threaded connection mechanism 10.

[0023] The bottle cap 31 matches the bottle mouth 8 , and after the preparation is completed, the bottle cap 31 can be screwed onto the bottle mouth 8 to seal and preserve the culture medium.

[0024] The blade control device 32 is a retractable hose. In the retracted state, the cutting blade 33 is located inside the hose to prevent it from cutting the bag body 1 when not in use. The hose can be extended under the action of external force, so that the cutting blade 33 extends out of the hose and cuts the bottom surface 12 of the bag body, thereby realizing the rapid preparation of dry powder culture medium.

[0025] The cutter head control device 32 is arranged outside the dry powder bin 6 .

[0026] The cutting blade 33 is made of non-conical tetrafluoro plastic, and after cutting the bottom surface 12 of the bag body, the bag remains connected to the culture bottle 9 and does not fall off.

[0027] The material of the bag body 1 of the filling bag is selected from polyethylene.

[0028] The bag body 1 of the loading bag is a structure formed by a double-layer base film layer, and the telescopic cutting mechanism 3 is arranged on one of the base film layers. Before loading, the double-layer base film layer is sealed by a heat sealing line 2 except for the position of the feed port 4. After loading, the feed port 4 of the bag body 1 is sealed by heat sealing welding, so that the bag body 1 of the loading bag becomes a closed structure.

[0029] When in use, the solvent is injected into the culture bottle 9 through the bottle mouth 8, the loading bag body 1 and the culture bottle 9 are connected by a threaded connection mechanism 10, the dry powder culture medium and the buffer substance are respectively injected into the dry powder bin 6 and the storage cavity 7 through the feed port 4, and the four sides are heat-sealed to form a heat-sealed line 2 to seal the loading bag body 1, and the whole is irradiated for sterilization. When preparing the culture medium, unscrew the bottle cap 31, extend the hose 32 of the telescopic cutting mechanism 3, extend the cutting blade 33 out of the hose and cut the bottom surface 12 of the bag body of the loading bag, then invert the device to make the solvent in the culture bottle 9 flow into the dry powder bin 6, turn it upside down several times until the material is completely dissolved and mixed evenly, squeeze the dry powder bin 6 hard to increase the internal pressure and squeeze the virtual welding line 11, or pull hard on both sides of the dry powder bin 6 to open the bag body of the loading bag near it, and then pull the virtual welding line 11, so that the dry powder bin 6 and the storage chamber 7 are connected, and use the same method to turn the device upside down until the buffer material in the storage chamber 7 is completely dissolved and mixed evenly. After the material is completely dissolved, place the entire device vertically on a horizontal surface, screw the bottle cap 31 on the bottle mouth 8 of the culture bottle 9, and the culture medium preparation is completed. Because the solvent, dry powder culture medium, and solvent are stored in separate chambers, the validity period is longer than that of liquid culture medium, and they are stored using an integrated device to maintain a sterile state, and can be quickly prepared when used, and the operation is convenient.

[0030] The above disclosure is only the preferred embodiment of the utility model, and does not limit the utility model in other forms. Those skilled in the art can understand that the utility model is not limited to each specific implementation. For those skilled in the art, without departing from the technical principle of the utility model, it is possible to make improvements or modifications within the scope of the utility model, and the various technical features mentioned in various places in the specification can be combined with each other, and these improvements or modifications should be included in the protection scope of the utility model.

Claims

1. An integrated storage and quick-mixing device for dry powder culture medium, comprising a filling bag body (1) and a culture bottle (9) connected to the filling bag body (1), characterized in that: The bag body (1) of the filling bag is provided with a virtual welding line (11) to divide the interior thereof into a dry powder bin (6) and a storage cavity (7); the virtual welding line (11) can be opened under the action of an external force to allow the storage cavity (7) to communicate with the dry powder bin (6); the dry powder bin (6) and the storage cavity (7) are respectively provided with a sealable feeding port (4); the side wall of the dry powder bin (6) is provided with a telescopic cutting mechanism (3); the outer wall of the telescopic cutting mechanism (3) is provided with a thread; the bottle cap (31) is connected to the telescopic cutting mechanism (3) via the thread; the bottle cap (31) is connected to the telescopic cutting mechanism (3) via the thread; 1) is located outside the dry powder bin (6), a telescopic cutting mechanism (3) is provided with a blade control device (32) and a cutting blade (33) inside, and a threaded connection mechanism (10) matching the mouth of the culture bottle (8) is provided at the bottom of the bag body (1) of the loading bag, and the blade control device (32) is used to control the movement of the cutting blade (33), so that the cutting blade (33) can destroy the bottom surface (12) of the bag body of the loading bag at the mouth of the bottle (8) under the action of external force, thereby connecting the dry powder bin (6) with the culture bottle (9).

2. The integrated storage and quick-mixing device for dry powder culture medium according to claim 1, characterized in that: The filling bag body (1) and the culture bottle (9) are connected via a threaded connection mechanism (10).

3. The integrated storage and quick-mixing device for dry powder culture medium according to claim 1, characterized in that: The bottle cap (31) matches the bottle mouth (8), and after the preparation is completed, the bottle cap (31) can be screwed onto the bottle mouth (8) to seal and preserve the culture medium.

4. The integrated storage and quick-mixing device for dry powder culture medium according to claim 1, characterized in that: The cutter head control device (32) is a retractable hose. In the retracted state, the cutting cutter head (33) is located inside the hose to prevent it from cutting through the bag body (1) when not in use. The hose can be extended under the action of an external force, so that the cutting cutter head (33) extends out of the hose to cut through the bottom surface (12) of the bag body.

5. The integrated storage and quick-mixing device for dry powder culture medium according to claim 1, characterized in that: The blade control device (32) is arranged outside the dry powder bin (6).

6. The integrated storage and quick-mixing device for dry powder culture medium according to claim 1, characterized in that: The cutting blade (33) is made of non-conical tetrafluoro plastic and can keep the bottom surface (12) of the bag body of the loading bag connected to the culture bottle (9) without falling off.

7. The integrated storage and quick-mixing device for dry powder culture medium according to claim 1, characterized in that: The material of the bag body (1) is selected from polyvinyl chloride, polyethylene, polyethylene terephthalate, ethylene-vinyl acetate copolymer or polypropylene.

8. The integrated storage and quick-mixing device for dry powder culture medium according to claim 1, characterized in that: The bag body (1) of the charging bag is a structure formed by a double-layer base film layer, and the telescopic cutting mechanism (3) is arranged on one of the base film layers. Before loading, the surroundings of the double-layer base film layer of the bag body (1) are sealed by a heat sealing line (2) except for the position of the feed port (4). After loading, the feed port (4) of the bag body (1) is sealed by heat sealing welding, so that the bag body (1) of the charging bag becomes a closed structure.

9. The integrated storage and quick-mixing device for dry powder culture medium according to claim 1, characterized in that: The virtual welding line (11) can be opened by squeezing the dry powder bin (6), and the squeezing increases the pressure inside the dry powder bin (6) so as to squeeze the virtual welding line (11) open, thereby connecting the material storage cavity (7) with the dry powder bin (6); Alternatively, the virtual welding line (11) can be opened by pulling the bag body (1) near the virtual welding line to both sides, so that the material storage cavity (7) and the dry powder bin (6) are connected.

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