Needle tube type nucleic acid extraction device

Abstract

The utility model discloses a needle tube type nucleic acid extraction device which comprises a first barrel, a second barrel, a core rod, a first liquid bag, a first spine, a second liquid bag, a second spine, an extraction tube and a silicon substrate membrane, and the second barrel is arranged at one end of the first barrel in a penetrating mode and is in sliding connection with the first barrel; the core rod penetrates through the end, located outside the first barrel, of the second barrel and is in sliding connection with the second barrel. The first liquid bag sleeves one end of the second barrel; the first spine is arranged in the first cylinder body; the second liquid bag sleeves one end of the core rod; the second spine is arranged in the second cylinder body; the extraction pipe is connected to one end, far away from the second barrel, of the first barrel and has the same axial direction as the first direction; the silicon matrix membrane is fixed at one end of the extraction tube away from the first barrel; wherein one of the cleaning liquid and the eluent is arranged in the first liquid bag, and the other one of the cleaning liquid and the eluent is arranged in the second liquid bag. The operation of obtaining the nucleic acid mixed solution through the needle tube type nucleic acid extraction device is convenient and rapid.

Description

Technical Field

[0001] The present application relates to the technical field of nucleic acid extraction, and in particular to a needle-tube type nucleic acid extraction device. Background Art

[0002] At present, nucleic acids are essential polymeric macromolecules for all known life forms. In order to test nucleic acids, they need to be extracted, and the commonly used method is the column method.

[0003] The principle of column-based nucleic acid extraction is to specifically adsorb the nucleic acid released by lysis onto a specific silicon carrier, which has a strong affinity and adsorption capacity only for nucleic acid, and basically does not adsorb other biochemical components such as proteins, polysaccharides, and lipids. The nucleic acid adsorbed on the silicon carrier is then eluted with an eluent to obtain a nucleic acid mixture, and then the nucleic acid mixture is separated to obtain purified nucleic acid. This method has the problem of complex operation. Utility Model Content

[0004] The main purpose of the present application is to provide a needle-tube type nucleic acid extraction device, aiming to solve the problem of complex operation of obtaining a mixed solution of nucleic acid.

[0005] To achieve the above-mentioned purpose, the present application provides a needle-tube type nucleic acid extraction device, which includes a first barrel, a second barrel, a core rod, a first liquid bag, a first spike, a second liquid bag, a second spike, an extraction tube and a silicon matrix membrane, the second barrel is inserted through one end of the first barrel and is slidably connected to the first barrel, the axial direction of the second barrel is the same as the first direction and has the freedom to slide along the first direction, and the first direction is the same as the axial direction of the first barrel; the core rod is inserted through one end of the second barrel located outside the first barrel and is slidably connected to the second barrel, the axial direction of the core rod is the same as the first direction and has the freedom to slide along the first direction degree; the first liquid bag is arranged on one end of the second cylinder located in the first cylinder; the first spike is arranged on the end of the first cylinder opposite to the second cylinder and is located on the moving path of the first liquid bag; the second liquid bag is arranged on one end of the core rod located in the second cylinder; the second spike is arranged on the end of the second cylinder opposite to the core rod and is located on the moving path of the second liquid bag; the extraction tube is detachably connected to one end of the first cylinder away from the second cylinder and the axial direction is the same as the first direction; the silicon matrix membrane is fixed to one end of the extraction tube away from the first cylinder; wherein, one of the cleaning liquid or the elution liquid is arranged in the first liquid bag, and the other is arranged in the second liquid bag.

[0006] Optionally, the needle-tube nucleic acid extraction device also includes a first cone head and a connecting tube, wherein the first cone head is arranged at one end of the first cylinder away from the second cylinder and is connected to the first cylinder; the connecting tube is sleeved on the outer circumference of the first cone head and is fixed to the first cylinder; wherein the extraction tube is threadedly connected to the connecting tube.

[0007] Optionally, the needle-tube nucleic acid extraction device also includes a second cone head, a first limiting ring and a first piston, the second cone head is arranged at one end of the second cylinder body located in the first cylinder body and is connected to the second cylinder body; the first limiting ring is sleeved on the outer circumference of the second cone head; the first piston is sleeved on the outer circumference of the second cone head and is located between the first limiting ring and the second cylinder body, and the outer circumference of the first piston is in contact with the inner circumference of the first cylinder body; wherein the first liquid bag is sleeved on the outer circumference of the second cone head and is located between the first limiting ring and the first piston.

[0008] Optionally, the needle-tube nucleic acid extraction device also includes a protrusion, a second limiting ring and a second piston, the protrusion is fixed to one end of the core rod located in the second cylinder; the second limiting ring is sleeved on the end of the protrusion away from the core rod; the second piston is sleeved on the outer periphery of the protrusion and is located between the second limiting ring and the core rod, and the outer periphery of the second piston is in contact with the inner periphery of the second cylinder; wherein the second liquid bag is sleeved on the outer periphery of the protrusion and is located between the second limiting ring and the second piston.

[0009] Optionally, the orthographic projection of the first spike along the first direction on the first liquid bag is a first projection, the orthographic projection of the first limiting ring along the first direction on the first liquid bag is a second projection, and the first projection is separated from the second projection; the orthographic projection of the second spike along the first direction on the second liquid bag is a third projection, the orthographic projection of the second limiting ring along the first direction on the second liquid bag is a fourth projection, and the third projection is separated from the fourth projection.

[0010] Optionally, the first piston is transitionally fitted with the inner circumference of the first cylinder; the second piston is interference fitted with the inner circumference of the second cylinder; and a cleaning liquid is provided in the first liquid bag.

[0011] Optionally, a plurality of first drainage grooves are provided on the side of the first limiting ring away from the first piston, and each first drainage groove extends radially along the first limiting ring; a plurality of second drainage grooves are provided on the side of the second limiting ring away from the second piston, and each second drainage groove extends radially along the second limiting ring.

[0012] Optionally, the needle-tube nucleic acid extraction device also includes a third piston, which is arranged at one end of the first cylinder body close to the first cone head, and is provided with a through hole extending along the first direction; wherein the through hole connects the first cone head and the second cone head.

[0013] Optionally, the needle-tube nucleic acid extraction device further comprises a curling edge and a pressing handle, wherein the curling edge is fixed to an end of the first barrel away from the first cone head; and the pressing handle is fixed to an end of the core rod outside the second barrel.

[0014] Optionally, the first spike is fixed to the side of the third piston facing the first liquid bag; the second spike is fixed to the end of the second cylinder close to the second cone head; when the first limiting ring is in contact with the third piston, there is a gap between the first spike and the first piston in the first direction; when the second limiting ring is in contact with the end of the second cylinder close to the second cone head, there is a gap between the second spike and the second piston in the first direction.

[0015] The embodiment of the present application provides a needle-tube type nucleic acid extraction device. When in use, the first direction can be the same as the gravity direction. After the sample and the lysis solution are mixed, they are added to the extraction tube. At this time, the waste liquid passes through the silicon matrix membrane, and the nucleic acid and impurities are intercepted by the silicon matrix membrane. Then, the first cylinder body and the extraction tube are connected. If the first liquid bag is provided with a cleaning solution, the second cylinder body is first pushed to make the first spike pierce the first liquid bag, and the cleaning solution flows into the extraction tube to wash the impurities from the silicon matrix membrane. Then, the core rod is pushed to make the second spike pierce the second liquid bag, and the elution solution flows into the extraction tube to elute the nucleic acid from the silicon matrix membrane, so that a nucleic acid mixture can be obtained. If the second liquid bag is provided with a cleaning solution, the core rod is first pushed to make the second spike pierce the second liquid bag, and the cleaning solution flows into the extraction tube to wash the impurities from the silicon matrix membrane. Then, the second cylinder body is pushed to make the first spike pierce the first liquid bag, and the elution solution flows into the extraction tube to elute the nucleic acid from the silicon matrix membrane, so that a nucleic acid mixture can be obtained. The overall operation is convenient and fast. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A schematic diagram of the overall structure of a needle-tube type nucleic acid extraction device provided in an embodiment of the present application;

[0017] Figure 2 for Figure 1 A cross-sectional view of an embodiment of the present invention;

[0018] Figure 3 for Figure 1 Schematic diagram of the structural disassembly of the embodiment in FIG.

[0019] In the figure: 1. first cylinder; 11. first cone head; 12. connecting cylinder; 13. third piston; 131. through hole; 2. second cylinder; 21. second cone head; 22. first limit ring; 23. first piston; 3. core rod; 31. raised portion; 32. second limit ring; 33. second piston; 4. first liquid bag; 5. first spike; 6. second liquid bag; 7. second spike; 81. curling; 82. pressing hand.

[0020] The realization of the purpose, functional features and advantages of this application will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0023] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the utility model.

[0025] refer to Figure 1 to Figure 3 It should be understood that Figure 3 The connections between the components should be like Figure 1 As compact as in the example, this is just an example. Figure 3Some components are disassembled and displayed for easy understanding. The embodiment of the present application provides a needle-tube type nucleic acid extraction device, which may include a first barrel 1, a second barrel 2, a core rod 3, a first liquid bag 4, a first spike 5, a second liquid bag 6, a second spike 7, an extraction tube and a silicon matrix membrane. The second barrel 2 is penetrated at one end of the first barrel 1 and is slidably connected to the first barrel 1. The axial direction of the second barrel 2 is the same as the first direction and has the freedom to slide along the first direction. The first direction is the same as the axial direction of the first barrel 1; the core rod 3 is penetrated at one end of the second barrel 2 located outside the first barrel 1 and is slidably connected to the second barrel 2. The axial direction of the core rod 3 is the same as the first direction and has the freedom to slide along the first direction. The first liquid bag 4 is sleeved on the end of the second barrel 2 located in the first barrel 1; the first spike 5 is arranged at the end of the first barrel 1 opposite to the second barrel 2 and is located on the moving path of the first liquid bag 4; the second liquid bag 6 is sleeved on the end of the core rod 3 located in the second barrel 2; the second spike 7 is arranged at the end of the second barrel 2 opposite to the core rod 3 and is located on the moving path of the second liquid bag 6; the extraction tube is detachably connected to the end of the first barrel 1 away from the second barrel 2 and the axial direction is the same as the first direction; the silicon matrix membrane is fixed to the end of the extraction tube away from the first barrel 1; wherein, one of the cleaning liquid or the elution liquid is arranged in the first liquid bag 4, and the other is arranged in the second liquid bag 6.

[0026] The present application embodiment provides a needle-tube type nucleic acid extraction device, such as Figure 1 As shown, the first direction is the X direction. When in use, the first direction X can be the same as the gravity direction. After the sample and the lysate are mixed, they are added to the extraction tube. At this time, the waste liquid passes through the silicon matrix membrane, and the nucleic acid and impurities are intercepted by the silicon matrix membrane. Then, the first cylinder 1 and the extraction tube are connected. If the first liquid bag 4 is provided with a cleaning liquid, the second cylinder 2 is first pushed to make the first spike 5 pierce the first liquid bag 4, and the cleaning liquid flows into the extraction tube to wash the impurities from the silicon matrix membrane. Then, the core rod 3 is pushed to make the second spike 7 pierce the second liquid bag 6, and the elution liquid flows into the extraction tube to elute the nucleic acid from the silicon matrix membrane, so that a nucleic acid mixture can be obtained; if the second liquid bag 6 is provided with a cleaning liquid, the core rod 3 is first pushed to make the second spike 7 pierce the second liquid bag 6, and the cleaning liquid flows into the extraction tube to wash the impurities from the silicon matrix membrane. Then, the second cylinder 2 is pushed to make the first spike 5 pierce the first liquid bag 4, and the elution liquid flows into the extraction tube to elute the nucleic acid from the silicon matrix membrane, so that a nucleic acid mixture can be obtained. The overall operation is convenient and fast.

[0027] refer to Figure 2 and Figure 3In an exemplary embodiment, the needle-tube nucleic acid extraction device may also include a first cone head 11 and a connecting tube 12, wherein the first cone head 11 is arranged at one end of the first barrel 1 away from the second barrel 2 and is connected to the first barrel 1; the connecting tube 12 is sleeved on the outer periphery of the first cone head 11 and is fixed to the first barrel 1; wherein the extraction tube is threadedly connected to the connecting tube 12.

[0028] Specifically, the inner circumference of the connecting tube 12 is provided with threads, the outer circumference of the extraction tube is provided with threads, one end of the extraction tube is threadedly connected to the inner circumference of the connecting tube 12 , and the extraction tube is also located at the outer circumference of the first cone head 11 .

[0029] It should be understood that the threads on the connecting tube 12 can be arranged on the outer periphery of the connecting tube 12 , and the threads on the extraction tube can be arranged on the inner periphery of the extraction tube, and one end of the extraction tube can be threadedly connected to the outer periphery of the connecting tube 12 .

[0030] It should be noted that the first barrel 1 and the first cone head 11 can be understood as a structure similar to a syringe, and the cleaning liquid or the eluent can flow from the first barrel 1 into the extraction tube through the first cone head 11.

[0031] refer to Figure 2 and Figure 3 In an exemplary embodiment, the needle-tube nucleic acid extraction device may also include a second cone head 21, a first limiting ring 22 and a first piston 23, wherein the second cone head 21 is arranged at one end of the second cylinder 2 located inside the first cylinder 1 and is connected to the second cylinder 2; the first limiting ring 22 is sleeved on the outer periphery of the second cone head 21; the first piston 23 is sleeved on the outer periphery of the second cone head 21 and is located between the first limiting ring 22 and the second cylinder 2, and the outer periphery of the first piston 23 is in contact with the inner periphery of the first cylinder 1; wherein the first liquid bag 4 is sleeved on the outer periphery of the second cone head 21 and is located between the first limiting ring 22 and the first piston 23.

[0032] Specifically, the second barrel 2 and the second cone head 21 can be understood here as a structure similar to a syringe. The cleaning liquid or the eluent can flow from the second barrel 2 into the first barrel 1 through the second cone head 21. The first liquid bag 4 is sleeved on the outer periphery of the second cone head 21. Pushing the second barrel 2 can drive the second cone head 21 and the first liquid bag 4 to approach the first spike 5 along the first direction X, thereby piercing the first liquid bag 4, so that the cleaning liquid or the eluent flows from the first liquid bag 4 into the interior of the first barrel 1, and finally flows into the extraction tube.

[0033] Furthermore, the first limiting ring 22 and the second cone head 21 can be threadedly connected, so that after the first liquid bag 4 is used, the second cylinder 2 can be pulled out from the first cylinder 1, and then the first limiting ring 22 can be unscrewed, and the first liquid bag 4 can be removed from the second cone head 21 for replacement. After the replacement is completed, the first limiting ring 22 can be screwed on and the corresponding part of the second cylinder 2 can be reinserted into the first cylinder 1 for next use.

[0034] Among them, pushing the second cylinder 2 to drive the first piston 23 to move can apply pressure to the silicon-based membrane, thereby improving the filtering effect of the silicon-based membrane.

[0035] refer to Figure 2 and Figure 3 In an exemplary embodiment, the needle-tube nucleic acid extraction device may also include a protrusion 31, a second limiting ring 32 and a second piston 33, wherein the protrusion 31 is fixed to one end of the core rod 3 located in the second cylinder 2; the second limiting ring 32 is sleeved on the end of the protrusion 31 away from the core rod 3; the second piston 33 is sleeved on the outer periphery of the protrusion 31 and is located between the second limiting ring 32 and the core rod 3, and the outer periphery of the second piston 33 is in contact with the inner periphery of the second cylinder 2; wherein the second liquid bag 6 is sleeved on the outer periphery of the protrusion 31 and is located between the second limiting ring 32 and the second piston 33.

[0036] Specifically, the second liquid bag 6 is sleeved on the outer periphery of the protrusion 31, and pushing the core rod 3 can drive the protrusion 31 and the second liquid bag 6 to approach the second spike 7 along the first direction X, thereby piercing the second liquid bag 6, so that the cleaning liquid or eluent flows from the second liquid bag 6 into the interior of the second cylinder 2, and finally flows into the extraction tube.

[0037] Furthermore, the second limiting ring 32 and the raised portion 31 can be threadedly connected, so that after the second liquid bag 6 is used, the core rod 3 can be pulled out from the second cylinder 2, and then the second limiting ring 32 can be unscrewed, and the second liquid bag 6 can be removed from the raised portion 31 for replacement. After the replacement is completed, the second limiting ring 32 can be screwed on and the corresponding part of the core rod 3 can be reinserted into the second cylinder 2 for next use.

[0038] Among them, pushing the core rod 3 to drive the second piston 33 to move can apply pressure to the silicon-based membrane, thereby improving the filtering effect of the silicon-based membrane.

[0039] refer to Figure 2 In the exemplary embodiment, the orthographic projection of the first spike 5 along the first direction X on the first liquid bag 4 is a first projection, the orthographic projection of the first limiting ring 22 along the first direction X on the first liquid bag 4 is a second projection, and the first projection is separated from the second projection; the orthographic projection of the second spike 7 along the first direction X on the second liquid bag 6 is a third projection, and the orthographic projection of the second limiting ring 32 along the first direction X on the second liquid bag 6 is a fourth projection, and the third projection is separated from the fourth projection.

[0040] Specifically, when the first projection is separated from the second projection, the second cylinder 2 is pushed along the first direction X to make the first liquid bag 4 close to the first spike 5, and the first spike 5 will not be blocked by the first limiting ring 22, ensuring that the first spike 5 can pierce the first liquid bag 4; when the third projection is separated from the fourth projection, the core rod 3 is pushed along the first direction X to make the second liquid bag 6 close to the second spike 7, and the second spike 7 will not be blocked by the second limiting ring 32, ensuring that the second spike 7 can pierce the second liquid bag 6.

[0041] refer to Figure 2 In the exemplary embodiment, the first piston 23 is transitionally fitted with the inner circumference of the first cylinder 1; the second piston 33 is interference fit with the inner circumference of the second cylinder 2; and a cleaning liquid is provided in the first liquid bag 4.

[0042] Specifically, when the first piston 23 is transitionally fitted with the inner circumference of the first cylinder 1, and the second piston 33 is interference fit with the inner circumference of the second cylinder 2, the friction force between the second piston 33 and the second cylinder 2 is greater than the friction force between the first piston 23 and the first cylinder 1. At this time, a cleaning liquid is set in the first liquid bag 4, and an elution liquid is set in the second liquid bag 6. When working in this way, it is only necessary to push the core rod 3 to drive the second cylinder 2 to move along the first direction X through the second piston 33, the first liquid bag 4 is punctured, and the cleaning liquid flows out. At this time, in the first direction X, the second cone head 21 conflicts with the first cylinder 1, and the second cylinder 2 cannot be pushed further. Continuing to push the core rod 3 will drive the core rod 3 and the second cylinder 2 to move relative to each other, so that the second liquid bag 6 is punctured, and the elution liquid flows out to finally obtain a mixed solution of nucleic acid. All operations can be completed by pushing the core rod 3, and the work is more convenient and quick.

[0043] In an exemplary embodiment, a plurality of first drainage grooves are provided on a side of the first limiting ring 22 away from the first piston 23, and each first drainage groove extends radially along the first limiting ring 22; a plurality of second drainage grooves are provided on a side of the second limiting ring 32 away from the second piston 33, and each second drainage groove extends radially along the second limiting ring 32.

[0044] Specifically, in the first direction X, when the second cone head 21 contacts the first barrel 1, the eluent or cleaning solution in the first barrel 1 can still flow into the first cone head 11 through the first drainage groove, ensuring that the needle-tube nucleic acid extraction device can be used normally.

[0045] It should be understood that in the first direction X, when the protrusion 31 contacts the second barrel 2, the eluent or cleaning solution in the second barrel 2 can still flow into the second cone head 21 through the second drainage groove, ensuring that the needle-tube nucleic acid extraction device can be used normally.

[0046] refer to Figure 2In an exemplary embodiment, the needle-tube nucleic acid extraction device may further include a third piston 13, which is disposed in the first cylinder 1 at one end close to the first cone head 11, and the third piston 13 is provided with a through hole 131 extending along the first direction X; wherein the through hole 131 connects the first cone head 11 and the second cone head 21.

[0047] Specifically, combined with the above-mentioned solution of only pushing the core rod 3, pushing the core rod 3 will drive the second cone head 21 to collide with the third piston 13, and then continue to push the core rod 3 to drive the second liquid bag 6 close to the second spike 7. At this time, the pressure between the second cone head 21 and the first cylinder 1 is absorbed by the third piston 13, preventing the first cylinder 1 or the second cone head 21 from being damaged by force, thereby improving the service life of the needle-tube nucleic acid extraction device.

[0048] refer to Figure 1 and Figure 3 In an exemplary embodiment, the needle-tube nucleic acid extraction device may further include a curling edge 81 and a pressing handle 82 , wherein the curling edge 81 is fixed to an end of the first barrel 1 away from the first cone head 11 ; and the pressing handle 82 is fixed to an end of the core rod 3 located outside the second barrel 2 .

[0049] Specifically, when in use, the index finger and the middle finger contact the side of the curling edge 81 away from the pressing hand 82, and the thumb presses the pressing hand 82 to push the core rod 3 with one hand, which is more convenient to use.

[0050] In an exemplary embodiment, the first spike 5 is fixed to the side of the third piston 13 facing the first liquid bag 4; the second spike 7 is fixed to one end of the second cylinder 2 close to the second cone head 21; when the first limiting ring 22 is in contact with the third piston 13, there is a gap between the first spike 5 and the first piston 23 in the first direction X; when the second limiting ring 32 is in contact with one end of the second cylinder 2 close to the second cone head 21, there is a gap between the second spike 7 and the second piston 33 in the first direction X.

[0051] Specifically, when the first limiting ring 22 conflicts with the third piston 13, a gap exists between the first spike 5 and the first piston 23 in the first direction X, thereby ensuring that the first piston 23 will not be damaged by the first spike 5; when the second limiting ring 32 conflicts with one end of the second cylinder 2 close to the second cone head 21, a gap exists between the second spike 7 and the second piston 33 in the first direction X, thereby ensuring that the second piston 33 will not be damaged by the second spike 7.

[0052] The above are only preferred embodiments of the present application, and are not intended to limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A needle-tube type nucleic acid extraction device, characterized in that: include: A first cylinder (1); A second cylinder (2) is inserted through one end of the first cylinder (1) and is slidably connected to the first cylinder (1); the axial direction of the second cylinder (2) is the same as the first direction and the second cylinder (2) has the freedom to slide along the first direction; the first direction is the same as the axial direction of the first cylinder (1); A core rod (3) is inserted through one end of the second cylinder (2) located outside the first cylinder (1) and is slidably connected to the second cylinder (2); the axial direction of the core rod (3) is the same as the first direction and has the freedom to slide along the first direction; A first liquid bag (4) is sleeved on one end of the second cylinder (2) located inside the first cylinder (1); A first spike (5) is arranged at an end of the first cylinder (1) opposite to the second cylinder (2) and is located on a moving path of the first liquid bag (4); A second liquid bag (6) is sleeved on one end of the core rod (3) located inside the second cylinder (2); A second spike (7) is arranged at an end of the second cylinder (2) opposite to the core rod (3) and is located on the moving path of the second liquid bag (6); An extraction tube, detachably connected to an end of the first cylinder (1) away from the second cylinder (2) and having an axial direction in the same direction as the first direction; A silicon matrix membrane is fixed to an end of the extraction tube away from the first cylinder (1); Wherein, one of the cleaning liquid and the eluting liquid is arranged in the first liquid bag (4), and the other is arranged in the second liquid bag (6).

2. The needle-tube type nucleic acid extraction device according to claim 1, characterized in that: The needle-tube type nucleic acid extraction device also includes: A first cone head (11) is arranged at one end of the first cylinder (1) away from the second cylinder (2) and is connected to the first cylinder (1); A connecting tube (12) is sleeved on the outer circumference of the first cone head (11) and fixed to the first cylinder body (1); Wherein, the extraction tube is threadably connected to the connecting tube (12).

3. The needle-tube type nucleic acid extraction device according to claim 2, characterized in that: The needle-tube type nucleic acid extraction device also includes: A second cone head (21) is arranged at one end of the second cylinder (2) located inside the first cylinder (1) and is in communication with the second cylinder (2); A first limiting ring (22) sleeved on the outer circumference of the second cone head (21); A first piston (23) is sleeved on the outer circumference of the second cone head (21) and is located between the first limiting ring (22) and the second cylinder (2), and the outer circumference of the first piston (23) is in contact with the inner circumference of the first cylinder (1); Wherein, the first liquid bag (4) is sleeved on the outer circumference of the second cone head (21) and is located between the first limiting ring (22) and the first piston (23).

4. The needle-tube type nucleic acid extraction device according to claim 3, characterized in that: The needle-tube type nucleic acid extraction device also includes: A raised portion (31) is fixed to one end of the core rod (3) located inside the second cylinder (2); A second limiting ring (32) is sleeved on an end of the protruding portion (31) away from the core rod (3); A second piston (33) is sleeved on the outer periphery of the protruding portion (31) and is located between the second limiting ring (32) and the core rod (3), and the outer periphery of the second piston (33) is in contact with the inner periphery of the second cylinder (2); Wherein, the second liquid bag (6) is sleeved on the outer periphery of the protruding portion (31) and is located between the second limiting ring (32) and the second piston (33).

5. The needle-tube type nucleic acid extraction device according to claim 4, characterized in that: The orthographic projection of the first spike (5) on the first liquid bag (4) along the first direction is a first projection, the orthographic projection of the first limiting ring (22) on the first liquid bag (4) along the first direction is a second projection, and the first projection is separated from the second projection; The orthographic projection of the second spike (7) on the second liquid bag (6) along the first direction is a third projection, the orthographic projection of the second limiting ring (32) on the second liquid bag (6) along the first direction is a fourth projection, and the third projection is separated from the fourth projection.

6. The needle-tube type nucleic acid extraction device according to claim 4, characterized in that: The first piston (23) is transitionally matched with the inner circumference of the first cylinder (1); The second piston (33) is interference fit with the inner circumference of the second cylinder (2); A cleaning liquid is provided in the first liquid bag (4).

7. The needle-tube type nucleic acid extraction device according to claim 4, characterized in that: A plurality of first drainage grooves are provided on a side of the first limiting ring (22) facing away from the first piston (23), and each of the first drainage grooves extends in a radial direction of the first limiting ring (22); A plurality of second drainage grooves are provided on a side of the second limiting ring (32) facing away from the second piston (33), and each of the second drainage grooves extends in the radial direction of the second limiting ring (32).

8. The needle-tube type nucleic acid extraction device according to claim 6, characterized in that: The needle-tube type nucleic acid extraction device also includes: A third piston (13) is arranged in the first cylinder (1) at one end close to the first cone head (11), and the third piston (13) is provided with a through hole (131) extending along the first direction; Wherein, the through hole (131) connects the first cone head (11) and the second cone head (21).

9. The needle-tube type nucleic acid extraction device according to claim 6, characterized in that: The needle-tube type nucleic acid extraction device also includes: A curling edge (81) fixed to an end of the first cylinder (1) away from the first cone head (11); A button (82) is fixed to one end of the core rod (3) located outside the second cylinder (2).

10. The needle-tube type nucleic acid extraction device according to claim 8, characterized in that: The first spike (5) is fixed to a side of the third piston (13) facing the first liquid bag (4); The second spike (7) is fixed to one end of the second cylinder (2) close to the second cone head (21); When the first limiting ring (22) contacts the third piston (13), a gap exists between the first spike (5) and the first piston (23) in the first direction; When the second limiting ring (32) contacts the end of the second cylinder (2) close to the second cone head (21), a gap exists between the second spike (7) and the second piston (33) in the first direction.

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