High-throughput automatic seed sample positioning device

Abstract

The utility model discloses a high-throughput automatic seed sample positioning device. The device comprises a cubic frame body rack, two lifting assemblies are symmetrically arranged on the two sides of the cubic frame body rack in a lifting mode and connected with the cubic frame body rack through horizontal connecting pieces, a PCR disc assembly is arranged on the top face of one lifting assembly, and a clamping jaw assembly is connected with the PCR disc assembly and used for clamping / loosening the PCR disc assembly. The longitudinal moving assembly is connected to the bottom of the clamping jaw assembly in a longitudinal moving mode and used for driving the clamping jaw assembly to move longitudinally, the side faces of the two ends of the transverse moving assembly are connected to the cubic frame body rack, and the top of the transverse moving assembly is connected to the bottom of the longitudinal moving assembly and used for driving the longitudinal moving assembly to move transversely; the method is used for processing seed samples. According to the utility model, the position information of the sampled seed matrix and the taken seed sample is accurately positioned, the matrix with high-quality genes can be specifically cultivated, the structure is simple, and the efficiency is improved.

Description

Technical Field

[0001] The utility model belongs to the field of agricultural engineering, and in particular relates to a high-throughput automated seed sample positioning device. Background Art

[0002] In biological breeding, it is necessary to sample a large number of seeds in order to obtain their genetic information and provide reference for breeders. After sampling, the seeds are divided into the sampled mother and the removed sample. After the sample is tested, the mother corresponding to the sample at the time of sampling will be traced back according to the test results, and the mother with high-quality genes will be cultivated in a targeted manner. For this reason, it is necessary to develop a high-throughput, automated sample positioning storage device to accurately store and record the location information of the sample. Utility Model Content

[0003] In order to solve the problems in the background technology, the utility model provides a high-throughput automated seed sample positioning device. The matching of the sampled mother and the removed sample is achieved through the cooperation of the lifting component, the longitudinal moving component and the lateral moving component, and the position information of the sample is accurately stored and recorded.

[0004] The technical solution adopted by the utility model is:

[0005] It includes a cubic frame frame with four sides connected;

[0006] It includes two lifting assemblies, which are symmetrically arranged on both sides of the cubic frame frame and can be lifted and lowered, and connected to the cubic frame frame via a horizontal connecting piece arranged at the lower part of the cubic frame frame;

[0007] including a PCR plate assembly horizontally arranged on a top surface of one of the lifting assemblies;

[0008] It includes a clamping claw assembly, which is arranged in the cavity of the cubic frame frame and connected to the PCR plate assembly, and is used to clamp / release the PCR plate assembly;

[0009] It includes a longitudinal moving assembly, which is longitudinally movably arranged in the cavity of the cubic frame frame, connected to the bottom of the clamping jaw assembly, and is used to drive the clamping jaw assembly to move longitudinally;

[0010] It includes a transverse moving assembly, which can be arranged in the cavity of the cubic frame frame in a transversely movable manner, with two side surfaces connected to the cubic frame frame and a top connected to the bottom of the longitudinal moving assembly, and is used to drive the longitudinal moving assembly to move transversely;

[0011] It comprises a sampling platform which is arranged on the top surface of the cubic frame and is used for processing seed samples.

[0012] The two lifting assemblies each include a vertical long slide and a tray; the bottom of the vertical long slide is fixed to one side of the cubic frame frame via a horizontal connecting piece, a lifting slider is slidably connected in the slide rail of the vertical long slide, a first motor for driving the lifting slider to move is provided at one end of the vertical long slide, the lifting slider is fixedly connected to a horizontally arranged tray, a material tray box is provided on the top surface of the tray of one of the lifting assemblies, the material tray box is a coverless box structure, and a PCR tray assembly is arranged inside the material tray box.

[0013] The PCR tray assembly includes two PCR trays, which are symmetrically and horizontally arranged inside the tray box, with a gap between the two PCR trays.

[0014] The transverse moving component includes a long transverse slide and a transverse slider. The long transverse slide is arranged in the cavity of the cubic frame frame. The two ends of the long transverse slide are respectively fixed to the middle of the two lifting components. The transverse slider is slidably connected in the transverse slide rail of the long transverse slide. One end of the long transverse slide is provided with a second electrode for driving the transverse slider to move. The longitudinal moving component is fixedly installed on the top of the transverse slider.

[0015] The longitudinal moving component includes a longitudinal long slide and a longitudinal slider; the longitudinal long slide is arranged in the cavity of the cubic frame frame and fixed to the top surface of the transverse slider, the longitudinal slider is slidably connected in the longitudinal slide rail of the longitudinal long slide, and a third motor for driving the longitudinal slider to move is provided at one end of the longitudinal long slide, and the clamping claw assembly is fixedly installed on the top of the longitudinal slider.

[0016] The clamping jaw assembly includes an electric clamping jaw, a second clamping member, a first clamping member and an extended connecting plate; one side of the extended connecting plate is horizontally fixed to the top surface of the longitudinal slider, and the other side of the top surface is fixed with the electric clamping jaw, and the two sides of the top surface of the electric clamping jaw are respectively provided with a horizontally and symmetrically arranged L-shaped second clamping member and a first clamping member, and the second clamping member and the first clamping member are used to be driven by the electric clamping jaw to move toward / oppositely and then clamp / release the tray box after the electric clamping jaw receives a command from the control platform.

[0017] A material dividing funnel is provided on the surface of the sampling platform, and the material dividing funnel is provided with two material dividing ports, one of which is used to connect to the sampling port of one of the PCR disks, and the other is used to connect to the sampling port of the other PCR disk; the horizontal distance between the centers of the two material dividing ports is equal to the horizontal distance between the centers of the two PCR disks.

[0018] The sides of the two lifting assemblies are respectively provided with a first photoelectric sensor and a second photoelectric sensor for detecting whether the material tray box reaches a designated position.

[0019] It also includes a control platform for controlling the displacement of the lifting assembly, the longitudinal moving assembly and the lateral moving assembly.

[0020] The beneficial effects of the utility model are:

[0021] 1. The position information of the seed mother body after sampling and the removed seed sample is accurately located through the cooperation of the lifting component, the longitudinal moving component and the lateral moving component, so that the mother body with high-quality genes can be cultivated in a targeted manner.

[0022] 2. The device has a simple structure, is easy to use, and improves efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a front view of the device of the utility model.

[0024] Figure 2 It is a structural diagram of the device of the utility model.

[0025] Figure 3 It is a rear view of the device of the utility model.

[0026] Figure 4 It is a structural schematic diagram of the blanking area of ​​the utility model;

[0027] In the figure: 11, vertical long slide, 12, first photoelectric sensor, 13, tray, 21, sampling table, 22, material distribution funnel, 23, longitudinal long slide, 24, transverse long slide, 25, electric clamp, 26, second clamp, 27, first clamp, 28, extended connecting plate, 32, second photoelectric sensor, 4, material tray box. DETAILED DESCRIPTION

[0028] The utility model is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0029] like Figure 1 and Figure 2 As shown, the high-throughput automated seed sample positioning device includes a cubic frame frame with four sides penetrated, two lifting assemblies are respectively arranged on both sides of the cubic frame frame and can be lifted and lowered, and the cubic frame frame is connected to the cubic frame frame via a horizontal connecting piece arranged at the lower part of the cubic frame frame, a PCR plate assembly is horizontally arranged on the top surface of one of the lifting assemblies, a clamping claw assembly is arranged in the cavity of the cubic frame frame and connected to the PCR plate assembly for clamping / releasing the PCR plate assembly, a longitudinal moving assembly can be longitudinally movably arranged in the cavity of the cubic frame frame, connected to the bottom of the clamping claw assembly, and used to drive the longitudinal displacement of the clamping claw assembly, a transverse moving assembly can be transversely movably arranged in the cavity of the cubic frame frame, the side surfaces at both ends are connected to the cubic frame frame, and the top is connected to the bottom of the longitudinal moving assembly, and is used to drive the transverse displacement of the longitudinal moving assembly, and a sampling table 21 is arranged on the top surface of the cubic frame frame for processing seed samples.

[0030] The two lifting assemblies each include a vertical long slide 11 and a tray 13; the bottom of the vertical long slide 11 is fixed to one side of the cubic frame frame via a horizontal connecting piece, a lifting slider is slidably connected in the slide rail of the vertical long slide 11, a first motor is installed in the horizontal connecting piece for driving the lifting slider, and the lifting slider is fixedly connected to a horizontally arranged tray 13, a tray box 4 is provided on the top surface of the tray 13 of one of the lifting assemblies, the tray box 4 is a coverless box structure, and a PCR tray assembly is arranged inside the tray box 4; Figure 4 As shown, the PCR plate assembly includes two 96-well PCR plates, and the two PCR plates are symmetrically and horizontally arranged inside the plate box 4, with a space between the two PCR plates.

[0031] like Figure 3 As shown, the lateral movement component includes a lateral long slide 24 and a lateral slider. The lateral long slide 24 is arranged in the cavity of the cubic frame frame. The two ends of the lateral long slide 24 are respectively fixed to the middle of the two lifting components. The lateral slider is slidably connected in the lateral slide rail of the lateral long slide 24. One end of the lateral long slide 24 is provided with a second electrode for driving the displacement of the lateral slider. The top of the lateral slider is fixedly installed with a longitudinal movement component.

[0032] The longitudinal moving assembly includes a longitudinal long slide 23 and a longitudinal slider; the longitudinal long slide 23 is arranged in the cavity of the cubic frame frame and fixed to the top surface of the transverse slider. The longitudinal slider is slidably connected in the longitudinal slide rail of the longitudinal long slide 23. A third motor for driving the displacement of the longitudinal slider is provided at one end of the longitudinal long slide 23, and a clamping claw assembly is fixed on the top of the longitudinal slider.

[0033] The clamping assembly includes an electric clamping member 25, a second clamping member 26, a first clamping member 27 and an extended connecting plate 28; one side of the extended connecting plate 28 is horizontally fixed to the top surface of the longitudinal slider, and the electric clamping member 25 is fixed to the top surface of the other side. The top surface of the electric clamping member 25 is respectively provided with horizontally and symmetrically arranged L-shaped second clamping members 26 and first clamping members 27 on both sides. The second clamping member 26 and the first clamping member 27 are used to be driven by the electric clamping member 25 to move toward / oppositely and then clamp / release the tray box 4 after the electric clamping member 25 receives the command issued by the control platform.

[0034] A material distribution funnel 22 is provided on the surface of the sampling table 21. The material distribution funnel 22 has two material distribution ports, one of which is used to connect to the sampling port of one PCR disk, and the other is used to connect to the sampling port of another PCR disk; the horizontal distance between the centers of the two material distribution ports is equal to the horizontal distance between the centers of the two PCR disks.

[0035] The sides of the two lifting components are respectively equipped with a first photoelectric sensor 12 and a second photoelectric sensor 32, which are used to detect whether the material tray box 4 reaches the specified position. Specifically, the first photoelectric sensor 12 is used to detect whether the material tray box 4 is located on the tray 13 on one side in the initial state, and the second photoelectric sensor 32 is used to detect whether the material tray box 4 is located on the tray 13 on the other side when the operation is completed.

[0036] It also includes a control platform, which is a computer, electrically connected to and controls the first motor, the second electrode and the third motor to drive the lifting slider, the horizontal slider and the longitudinal slider to move.

[0037] The working process of the utility model high-throughput automated seed sample positioning device is as follows:

[0038] like Figure 1 As shown in the A area in the figure, the tray box 4 containing two empty PCR trays is placed on the surface of the tray 13, and the control platform controls the first motor to drive, drive the lifting slider to move, and then drive the tray box 4 moved to the surface of the tray 13 to move to a certain height, which is at the same height as the second clamping member 26 and the first clamping member 27. At this time, the first photoelectric sensor 12 detects the position of the tray box 4, as shown in FIG. Figure 1 As shown in the B area, the control platform controls the second motor and the third motor to respectively drive the horizontal slider on the horizontal long slide 24 and the longitudinal slider on the longitudinal long slide 23 to move the clamping claw assembly horizontally and longitudinally to the tray box 4 on the surface of the tray 13 in sequence;

[0039] The control platform sends a signal, and after the electric clamp 25 receives the command sent by the control platform, it is driven by the electric clamp 25 to move toward each other and then clamp the tray box 4. After clamping the tray box 4, the control platform controls the second motor and the third motor to respectively drive the horizontal slider on the horizontal long slide 24 and the longitudinal slider on the longitudinal long slide 23 to move the clamp assembly and the tray box 4 horizontally and longitudinally to the bottom of the sampling platform 21. One of the material distribution ports is connected to the first sampling port of one of the 96-well PCR plates, and at this time, the other material distribution port is connected to the first sampling port of another 96-well PCR plate. Then, the seed sampling blade on the sampling platform 21 is used to cut the seeds. The seed matrix and the removed seed sample enter the first sampling port of the two 96-well PCR plates from the two material distribution ports respectively. After the seed falls into the sampling port and pauses for a while, the material tray box 4 moves and the two discharge ports are connected to the second sampling ports of the two 96-well PCR plates respectively. In sequence, the material tray box 4 moves in a tortuous path, that is, a serpentine path until the sampling ports of the two 96-well PCR plates are respectively filled with seed matrix and seed sample. Because the center distance of the symmetrical sampling ports of the two 96-well PCR plates is the same as the center distance of the two material distribution ports, the seed matrix and the seed sample correspond one to one, and the location information of the sample is accurately stored and recorded. After the seeds are filled, Figure 1As shown in the C area, the control platform controls the motor drive to drive the tray box 4 to move to the tray 13 on the other side of the cubic frame frame, and the second photoelectric sensor 32 detects the tray box 4 on the tray 13 on the other side, completing the operation.

Claims

1. A high-throughput automated seed sample positioning device, characterized in that: It includes a cubic frame frame with four sides connected; It includes two lifting assemblies, which are symmetrically arranged on both sides of the cubic frame frame and can be lifted and lowered, and connected to the cubic frame frame via a horizontal connecting piece arranged at the lower part of the cubic frame frame; including a PCR plate assembly horizontally arranged on a top surface of one of the lifting assemblies; It includes a clamping claw assembly, which is arranged in the cavity of the cubic frame frame and connected to the PCR plate assembly, and is used to clamp / release the PCR plate assembly; It includes a longitudinal moving assembly, which is longitudinally movably arranged in the cavity of the cubic frame frame, connected to the bottom of the clamping jaw assembly, and is used to drive the clamping jaw assembly to move longitudinally; It includes a transverse moving assembly, which can be arranged in the cavity of the cubic frame frame in a transversely movable manner, with two side surfaces connected to the cubic frame frame and a top connected to the bottom of the longitudinal moving assembly, and is used to drive the longitudinal moving assembly to move transversely; It comprises a sampling platform (21) which is arranged on the top surface of the cubic frame and is used for processing seed samples.

2. The high-throughput automated seed sample positioning device according to claim 1, characterized in that: The two lifting assemblies each comprise a vertical long slide (11) and a tray (13); the bottom of the vertical long slide (11) is fixed to one side of the cubic frame frame via a horizontal connecting piece, a lifting slider is slidably connected in the slide rail of the vertical long slide (11), a first motor for driving the lifting slider to move is provided at one end of the vertical long slide (11), the lifting slider is fixedly connected to a horizontally arranged tray (13), a material tray box (4) is provided on the top surface of the tray (13) of one of the lifting assemblies, the material tray box (4) is a coverless box structure, and a PCR tray assembly is arranged inside the material tray box (4).

3. The high-throughput automated seed sample positioning device according to claim 2, characterized in that: The PCR tray assembly comprises two PCR trays, which are symmetrically and horizontally arranged inside the tray box (4), with a space between the two PCR trays.

4. The high-throughput automated seed sample positioning device according to claim 1, characterized in that: The transverse moving assembly comprises a transverse long slide (24) and a transverse slider. The transverse long slide (24) is arranged in the cavity of the cubic frame frame. The two ends of the transverse long slide (24) are respectively fixed to the middle of the two lifting assemblies. The transverse slider is slidably connected in the transverse slide rail of the transverse long slide (24). A second electrode for driving the transverse slider to move is arranged at one end of the transverse long slide (24). The longitudinal moving assembly is fixedly installed on the top of the transverse slider.

5. The high-throughput automated seed sample positioning device according to claim 4, characterized in that: The longitudinal moving assembly comprises a longitudinal long slide (23) and a longitudinal slider; the longitudinal long slide (23) is arranged in the cavity of the cubic frame frame and fixed to the top surface of the transverse slider; the longitudinal slider is slidably connected in the longitudinal slide rail of the longitudinal long slide (23); a third motor for driving the longitudinal slider to move is arranged at one end of the longitudinal long slide (23); and the clamping claw assembly is fixedly mounted on the top of the longitudinal slider.

6. The high-throughput automated seed sample positioning device according to claim 5, characterized in that: The clamping jaw assembly comprises an electric clamping jaw (25), a second clamping member (26), a first clamping member (27) and an extended connecting plate (28); one side of the extended connecting plate (28) is horizontally fixed to the top surface of the longitudinal sliding block, and the other side of the top surface is fixed with the electric clamping jaw (25); the two sides of the top surface of the electric clamping jaw (25) are respectively provided with a horizontally and symmetrically arranged L-shaped second clamping member (26) and a first clamping member (27); the second clamping member (26) and the first clamping member (27) are used to be driven by the electric clamping jaw (25) to move in opposite directions to clamp / release the material tray box (4) after the electric clamping jaw (25) receives a command from the control platform.

7. The high-throughput automated seed sample positioning device according to claim 3, characterized in that: A material dividing funnel (22) is provided on the surface of the sampling platform (21), and the material dividing funnel (22) is provided with two material dividing ports, one of which is used to connect to the sampling port of one of the PCR disks, and the other is used to connect to the sampling port of the other PCR disk; the horizontal distance between the centers of the two material dividing ports is equal to the horizontal distance between the centers of the two PCR disks.

8. The high-throughput automated seed sample positioning device according to claim 1, characterized in that: A first photoelectric sensor (12) and a second photoelectric sensor (32) are respectively installed on the sides of the two lifting assemblies, and are used to detect whether the material tray box (4) has reached a specified position.

9. The high-throughput automated seed sample positioning device according to claim 1, characterized in that: It also includes a control platform for controlling the displacement of the lifting assembly, the longitudinal moving assembly and the lateral moving assembly.

Images