Solvent effect eliminator

Abstract

The utility model discloses a solvent effect eliminator, and relates to the technical field of chromatographic analysis, the solvent effect eliminator comprises a column sleeve, the left end and the right end of the column sleeve are both connected with filling adjusting pieces, an inner cavity of the column sleeve is fixedly connected with a partition plate, the inner cavity of the column sleeve is divided into two groups of mixing chambers through the partition plate, the two groups of mixing chambers are respectively and uniformly filled with steel balls, and the steel balls are arranged in the mixing chambers. A small hole is formed in the center of the partition plate. The size of the cavity structure of the mixing cavity in the column sleeve can be conveniently adjusted, and a proper amount of steel balls can be filled at any time to serve as obstacles, so that the influence of a solvent effect on detection analysis is eliminated.

Description

Technical Field

[0001] The utility model relates to the technical field of chromatographic analysis, in particular to a solvent effect eliminator. Background Art

[0002] High performance liquid chromatography is currently the most important means and method for production supervision and quality control in the pharmaceutical industry. When using high performance liquid chromatography for analysis, the sample is usually dissolved in a mobile phase, and then separated and analyzed in a chromatographic column. However, in the actual development process, many samples have to be dissolved in other solvents due to objective reasons such as poor solubility in the mobile phase, which causes problems such as peak front extension, decreased column efficiency, reduced separation, and inaccurate content calculation, which is the solvent effect.

[0003] Research has found that filling a certain amount of tiny bead-type obstacles in the solvent effect eliminator is beneficial to improving the mixing effect of the mobile phase and the sample solvent; but unlike the existing mobile phase mixers, a certain volume of cavity, i.e., dead volume, in the solvent effect eliminator is also beneficial to the online dilution effect of the mobile phase and the sample solvent. Therefore, how to reasonably optimize the cavity structure and fill the obstacles in an appropriate amount to eliminate the influence of the solvent effect on the detection analysis is a technical problem that has not yet been solved in this field.

[0004] Based on this, we propose a solvent effect eliminator. Utility Model Content

[0005] The purpose of the utility model is to overcome the above-mentioned problems existing in the prior art and to provide a solvent effect eliminator, which is convenient for adjusting the size of the cavity structure of the mixing chamber in the column sleeve and can be filled with an appropriate amount of steel balls as obstacles at any time to eliminate the influence of the solvent effect on the detection and analysis.

[0006] In order to achieve the above technical objectives and the above technical effects, the present invention is implemented through the following technical solutions:

[0007] The utility model discloses a solvent effect eliminator, comprising a column sleeve, both left and right ends of the column sleeve are connected with filling adjustment pieces, the inner cavity of the column sleeve is fixedly connected with a partition plate, the inner cavity of the column sleeve is divided into two groups of mixing chambers by the partition plate, the two groups of mixing chambers are respectively and evenly filled with steel balls, and a small hole is opened at the center of the partition plate; the filling adjustment piece comprises a bearing sleeve, an infusion tube and a sleeve plug, the bearing sleeve is arranged at the end of the column sleeve, the infusion tube passes through one end of the bearing sleeve, the sleeve plug can be slidably connected in the bearing sleeve, the sleeve plug seals the end of the column sleeve, a through hole is arranged at the center of the sleeve plug, and the infusion tube cooperates with the through hole.

[0008] Preferably, the filling adjustment member further comprises a sealing sleeve, one end of which is sleeved with the inner cavity of the bearing sleeve, and the other end of which is an integral structure with the column sleeve, and the sleeve plug seals the inner cavity of the sealing sleeve.

[0009] Preferably, the sleeve plug is interference fit with the inner cavity of the sealing sleeve, the outer wall of the bearing sleeve is symmetrically provided with two slide grooves, the outer sleeve of the bearing sleeve is provided with a ring, the ring is connected to the sleeve plug through two connecting bolts, and the connecting bolts are located in the slide groove.

[0010] Preferably, an inlet and outlet pipe is fixedly connected to the outer wall of the sealing sleeve, and the inner cavity of the inlet and outlet pipe is connected with the inner cavity of the sealing sleeve through an inlet and outlet, and the inlet and outlet are opened on the outer wall of the sealing sleeve.

[0011] Preferably, when the connecting bolt is located at an end of the slide slot away from the column sleeve, the end of the sleeve plug is located at a side of the inlet and outlet pipe away from the column sleeve.

[0012] Preferably, a pipe cover is screwed onto the outside of the inlet and outlet pipe, the inner cavity of the pipe cover is provided with a pipe plug matching the inlet and outlet, a positioning column is provided on the inner side of the pipe cover, and the pipe plug is sleeved on the positioning column.

[0013] The present invention divides the inner cavity of the column sleeve into two groups of mixing chambers by a partition plate, and uses a filling adjustment member for each mixing chamber, so that the size of the cavity structure of the mixing chamber in the column sleeve can be adjusted by the sleeve plug, and an appropriate amount of steel balls can be filled at any time through the inlet and outlet pipes.

[0014] Steel beads are used as obstacles to eliminate the influence of solvent effects on detection and analysis.

[0015] Specifically in practical use, the chromatographic column and the injector are connected respectively by the infusion tubes at both ends, and the chromatographic column and the injector are then connected to the high performance liquid chromatograph. During the high performance liquid chromatography analysis and preparation, the chromatographic system must be balanced with the mobile phase first. In this process, the mobile phase flows through the solvent effect eliminator of the present application and fills the cavity of the column sleeve. After the injection, the sample solvent flows through the cavity of the solvent effect eliminator of the present application together with the sample, and the steel ball acts as an obstacle to keep the mobile phase in the cavity. The sample solvent is then diluted by the mobile phase retained in the cavity. After the sample solvent is diluted by the mobile phase, it still mixes with the sample, so the "original sample solvent" and the "newly mixed mobile phase" constitute the "new sample solvent". The chemical composition of the "new sample solvent" is closer to the mobile phase, which is equivalent to replacing the original "diluting the original sample solution with the mobile phase" before the injection in principle with the online dilution method, which effectively eliminates and alleviates the large difference in elution ability between the mobile phase and the sample solvent, thereby achieving the effect of improving the peak shape, improving the column efficiency and improving the separation degree. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1It is a schematic diagram of the structure of the utility model;

[0017] Figure 2 It is an exploded view of the utility model;

[0018] Figure 3 It is a cross-sectional view of the utility model;

[0019] Figure 4 It is a partial cross-sectional view of the entrance and exit of the utility model in an open state;

[0020] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0021] 1-column sleeve, 201-sealing sleeve, 202-bearing sleeve, 203-ring, 204-displacement slot, 205-infusion tube, 206-connecting bolt, 207-inlet and outlet pipes, 208-positioning column, 209-tube cover, 210-tube plug, 211-inlet and outlet, 212-sleeve plug, 213-through hole, 3-partition plate, 4-small hole, 5-steel ball. DETAILED DESCRIPTION

[0022] The following is combined with Figure 1-4 The utility model is described in further detail.

[0023] An embodiment provided by the utility model:

[0024] like Figure 1 , Figure 2 , Figure 3 As shown, a solvent effect eliminator comprises a column sleeve 1, both left and right ends of the column sleeve 1 are connected with filling adjustment pieces, the inner cavity of the column sleeve 1 is fixed with a partition plate 3, the inner cavity of the column sleeve 1 is divided into two groups of mixing chambers by the partition plate 3, the two groups of mixing chambers are respectively and evenly filled with steel balls 5, and a small hole 4 is opened at the center of the partition plate 3.

[0025] The filling adjustment member includes a bearing sleeve 202, an infusion tube 205 and a sleeve plug 212. The bearing sleeve 202 is arranged at the end of the column sleeve 1, and the infusion tube 205 passes through one end of the bearing sleeve 202. The sleeve plug 212 can be slidably connected in the bearing sleeve 202. The sleeve plug 212 closes the end of the column sleeve 1. A through hole 213 is arranged in the center of the sleeve plug 212, and the infusion tube 205 cooperates with the through hole 213.

[0026] The filling adjustment member further comprises a sealing sleeve 201 , one end of which is sleeved with the inner cavity of the bearing sleeve 202 , and the other end of which is an integrated structure with the column sleeve 1 , and a sleeve plug 212 seals the inner cavity of the sealing sleeve 201 .

[0027] The sleeve plug 212 is interference fit with the inner cavity of the sealing sleeve 201, and the outer wall of the bearing sleeve 202 is symmetrically provided with two slide grooves 204. The outer side of the bearing sleeve 202 is provided with a ring 203, and the ring 203 is connected to the sleeve plug 212 through two connecting bolts 206, and the connecting bolts 206 are located in the slide groove 204.

[0028] An inlet and outlet pipe 207 is fixedly connected to the outer wall of the sealing sleeve 201 . The inner cavity of the inlet and outlet pipe 207 is connected to the inner cavity of the sealing sleeve 201 through an inlet and outlet 211 . The inlet and outlet 211 is opened on the outer wall of the sealing sleeve 201 .

[0029] When the connecting bolt 206 is located at the end of the slide groove 204 away from the column sleeve 1 , the end of the sleeve plug 212 is located at the side of the inlet and outlet pipe 207 away from the column sleeve 1 .

[0030] The outside of the inlet and outlet pipe 207 is screwed with a pipe cover 209 , the inner cavity of the pipe cover 209 is provided with a pipe plug 210 that matches the inlet and outlet 211 , and the inner side of the pipe cover 209 is provided with a positioning column 208 , and the pipe plug 210 is sleeved on the positioning column 208 .

[0031] like Figure 3 and Figure 4 As shown, the outer wall of the sealing sleeve 201 is fixedly connected with an inlet and outlet pipe 207, and the inner cavity of the inlet and outlet pipe 207 is connected with the inner cavity of the sealing sleeve 201 through the inlet and outlet 211. The inlet and outlet 211 is opened on the outer wall of the sealing sleeve 201, which is convenient for sending and taking out the steel balls in the column sleeve 1; the outside of the inlet and outlet pipe 207 is screwed with a pipe cover 209, and the inner cavity of the pipe cover 209 is provided with a pipe plug 210 matched with the inlet and outlet 211. A positioning column 208 is provided on the inner side of the pipe cover 209, and the pipe plug 210 is sleeved on the positioning column 208, which is convenient for fixing the connection between the pipe plug 210 and the pipe cover 209. The inlet and outlet 211 is conveniently blocked and sealed by the pipe plug 210, and then the inlet and outlet 211 is conveniently blocked and sealed by screwing and tightening the pipe cover 209 on the inlet and outlet pipe 207.

[0032] The present application divides the inner cavity of the column sleeve into two groups of mixing chambers by a partition plate, and uses a filling adjustment piece for each mixing chamber, so that the size of the cavity structure of the mixing chamber in the column sleeve is adjusted by the sleeve plug, and an appropriate amount of steel balls can be filled through the inlet and outlet pipes at any time.

[0033] like Figure 3 , Figure 4As shown, the inner cavity of the column sleeve 1 is divided into two groups of mixing chambers by the partition plate 3, and a filling adjustment member is used for each mixing chamber. On the filling adjustment member, the ring ring 203 is slid on the bearing sleeve 202 to make it move along the length direction of the bearing sleeve 202, driving the connecting bolt 206 to slide in the displacement groove 204, and further driving the sleeve plug 212 to move along the length direction in the sealing sleeve 201. When moving outward, the inlet and outlet 211 is opened, so that the inner cavity of the sealing sleeve 201 is connected with the inner cavity of the inlet and outlet pipe 207, and the mixing chamber is filled with liquid through the inlet and outlet pipe 207. After the steel balls are filled, the tube cover 209 is screwed and tightened on the inlet and outlet tube 207, so that the tube plug 210 seals and blocks the inlet and outlet 211, and then the sleeve plug 212 is moved inward in the sealing sleeve 201 and inserted into the mixing chamber to resist the filled steel balls, so that the sleeve plug 212 further seals the inlet and outlet 211, and the sleeve plug 212 is moved left and right to adjust the size of the cavity structure of the mixing chamber in the column sleeve 1, and an appropriate amount of steel balls can be filled at any time through the inlet and outlet 211 as obstacles to eliminate the influence of the solvent effect on the detection and analysis.

[0034] The steel balls are used as obstacles to eliminate the influence of solvent effect on the detection and analysis. In actual use, the two mixing chambers in the cylindrical column sleeve 1 of 2.1*5mm or 2.1*10mm are filled with steel balls with a diameter of 0.4-2mm, and the filling rate is 0-60%.

[0035] Specifically in practical use, the chromatographic column and the injector are connected respectively by the infusion tubes at both ends, and the chromatographic column and the injector are then connected to the high performance liquid chromatograph. During the high performance liquid chromatography analysis and preparation, the chromatographic system must be balanced with the mobile phase first. In this process, the mobile phase flows through the solvent effect eliminator of the present application and fills the cavity of the column sleeve. After the injection, the sample solvent flows through the cavity of the solvent effect eliminator of the present application together with the sample, and the steel ball acts as an obstacle to keep the mobile phase in the cavity. The sample solvent is then diluted by the mobile phase retained in the cavity. After the sample solvent is diluted by the mobile phase, it still mixes with the sample, so the "original sample solvent" and the "newly mixed mobile phase" constitute the "new sample solvent". The chemical composition of the "new sample solvent" is closer to the mobile phase, which is equivalent to replacing the original "diluting the original sample solution with the mobile phase" before the injection in principle with the online dilution method, which effectively eliminates and alleviates the large difference in elution ability between the mobile phase and the sample solvent, thereby achieving the effect of improving the peak shape, improving the column efficiency and improving the separation degree.

[0036] The above are all preferred embodiments of the present utility model, and are not intended to limit the protection scope of the present utility model. Therefore, any equivalent changes made based on the structure, shape, and principle of the present utility model should be included in the protection scope of the present utility model.

Claims

1. A solvent effect eliminator, characterized in that: The invention comprises a column sleeve (1), wherein both left and right ends of the column sleeve (1) are connected to a filling adjustment member, the inner cavity of the column sleeve (1) is fixedly connected to a partition plate (3), the inner cavity of the column sleeve (1) is divided into two groups of mixing chambers by the partition plate (3), the two groups of mixing chambers are respectively and evenly filled with steel balls (5), and a small hole (4) is opened at the center of the partition plate (3); the filling adjustment member comprises a bearing sleeve (202), an infusion tube (205) and a sleeve plug (212), the bearing sleeve (202) is arranged at the end of the column sleeve (1), the infusion tube (205) passes through one end of the bearing sleeve (202), the sleeve plug (212) can be slidably connected to the bearing sleeve (202), the sleeve plug (212) closes the end of the column sleeve (1), a through hole (213) is arranged at the center of the sleeve plug (212), and the infusion tube (205) and the through hole (213) cooperate.

2. A solvent effect eliminator according to claim 1, characterized in that: The filling adjustment member further comprises a sealing sleeve (201), one end of the sealing sleeve (201) being sleeved with the inner cavity of the bearing sleeve (202), the other end of the sealing sleeve (201) being an integrated structure with the column sleeve (1), and a sleeve plug (212) sealing the inner cavity of the sealing sleeve (201).

3. A solvent effect eliminator according to claim 2, characterized in that: The sleeve plug (212) is interference-fitted with the inner cavity of the sealing sleeve (201); the outer wall of the bearing sleeve (202) is symmetrically provided with two slide grooves (204); the outer sleeve of the bearing sleeve (202) is provided with a ring (203); the ring (203) is connected to the sleeve plug (212) via two connecting bolts (206); and the connecting bolts (206) are located in the slide grooves (204).

4. A solvent effect eliminator according to claim 3, characterized in that: An inlet and outlet pipe (207) is fixedly connected to the outer wall of the sealing sleeve (201); the inner cavity of the inlet and outlet pipe (207) is connected to the inner cavity of the sealing sleeve (201) via an inlet and outlet (211); and the inlet and outlet (211) is provided on the outer wall of the sealing sleeve (201).

5. The solvent effect eliminator according to claim 3, characterized in that: When the connecting bolt (206) is located at an end of the slide groove (204) away from the column sleeve (1), the end of the sleeve plug (212) is located at a side of the inlet and outlet pipe (207) away from the column sleeve (1).

6. A solvent effect eliminator according to claim 4, characterized in that: The exterior of the inlet and outlet pipe (207) is threadedly connected with a pipe cover (209), the inner cavity of the pipe cover (209) is provided with a pipe plug (210) that matches the inlet and outlet (211), a positioning column (208) is provided on the inner side of the pipe cover (209), and the pipe plug (210) is sleeved on the positioning column (208).

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