Protein chromatography device

Abstract

The utility model discloses a protein chromatography device. The protein chromatography device comprises a chromatography tank and a detection liquid injection pipe. The chromatography tank comprises a tank body, a first cavity and a second cavity, wherein the first cavity and the second cavity are arranged in the tank body. The bottom ends of the first cavity and the second cavity in the height direction of the tank body are flush. The first cavity and the second cavity are both used for filling protein column chromatography filler and a protein solution. And the detection liquid injection pipe penetrates through the wall thickness direction of the tank body and is communicated with the second cavity. And the detection liquid injection pipe is used for injecting detection liquid into the second cavity. The detection liquid is used for marking the target protein in the second cavity to display a separation and purification effect, and the separation effect of the target protein in the first cavity is reflected by the separation effect of the target protein in the second cavity. On the premise that the target protein in the first cavity is not affected, the separation condition of the target protein in the first cavity is reflected, and the convenience of knowing the separation and purification effect of the target protein in the chromatography process is improved.

Description

Technical Field

[0001] The present application relates to the technical field of protein chromatography, and in particular to a protein chromatography device. Background Art

[0002] Protein chromatography purification systems are often used to remove impurities from sample solutions and separate and purify target proteins. In the prior art, if you want to know the separation effect of the target protein during the experiment, you often need to use, for example, a UV detector or a conductivity detector to detect the separation effect, and the detection operation may lag behind the separation operation, making the experiment inconvenient. Utility Model Content

[0003] The present application provides a protein chromatography device to improve the convenience of obtaining the separation and purification effect of a target protein during the chromatography process.

[0004] The present application provides a protein chromatography device, comprising a chromatography tank and a detection liquid injection tube. The chromatography tank comprises a tank body, a first cavity and a second cavity arranged in the inner cavity of the tank body. The first cavity and the second cavity are not connected to each other, and the bottom ends of the first cavity and the second cavity along the height direction of the tank body are flush, and the first cavity and the second cavity are both used to fill protein column chromatography fillers and protein solutions to chromatograph the target protein in the protein solution. The detection liquid injection tube passes through the wall thickness direction of the tank body and is connected to the second cavity. The detection liquid injection tube is used to inject the detection liquid into the second cavity. The detection liquid is used to combine with the target protein in the protein solution in the second cavity and mark the target protein to show the separation effect of the target protein in the second cavity, and the separation effect of the target protein in the second cavity reflects the separation effect of the target protein in the first cavity.

[0005] In some embodiments, the volume of the first cavity is greater than the volume of the second cavity.

[0006] In some embodiments, the protein chromatography device further comprises a partition plate, wherein the partition plate divides the inner cavity of the tank into two regions in a cross section of the tank to form a first cavity and a second cavity respectively.

[0007] In some embodiments, the protein chromatography device further comprises a discharge device, which is connected to the bottom of the first cavity and the second cavity along the height direction, and is used to discharge the sample liquid formed by the protein solution in the first cavity and the second cavity after chromatography.

[0008] In some embodiments, the protein chromatography device further comprises a decompression device, which is connected to the first cavity and the second cavity, and is used to reduce the pressure of the solution in the first cavity and the second cavity to promote the sample liquid to be discharged through the discharge device.

[0009] In some embodiments, the decompression device includes a decompression pump connected to the first cavity and the second cavity and used to extract part of the sample liquid in the first cavity and the second cavity to reduce the pressure of the solution in the first cavity and the second cavity.

[0010] In some embodiments, the discharge device includes a first discharge pipe and a second discharge pipe. The bottom end of the first cavity along the height direction is connected to the first discharge pipe. The bottom end of the second cavity along the height direction is connected to the second discharge pipe.

[0011] In some embodiments, the protein chromatography device further comprises a heat-insulating sleeve. The heat-insulating sleeve is sleeved on the outer side of the tank body. There is a gap between the heat-insulating sleeve and the circumferential surface of the tank body. The gap is used to fill a heat-conducting medium to heat or cool the tank body.

[0012] In some embodiments, the protein chromatography device further comprises a temperature control device, which is connected to the heat-insulating sleeve and is used to adjust the temperature of the heat-conducting medium to increase or decrease.

[0013] In some embodiments, the protein chromatography device further comprises a temperature detector, which is disposed on the heat-insulating sleeve and is configured to detect and display the temperature of the heat-conducting medium in real time.

[0014] In some embodiments, at least a portion of the detection liquid injection tube passes through the thermal insulation sleeve and extends to the radial outside of the thermal insulation sleeve.

[0015] Based on the technical solution provided in the present application, the protein chromatography device includes a chromatography tank and a detection liquid injection tube. The chromatography tank includes a tank body, a first cavity and a second cavity arranged in the inner cavity of the tank body. The first cavity and the second cavity are not connected to each other, and the bottom ends of the first cavity and the second cavity along the height direction of the tank body are flush. The first cavity and the second cavity are both used to fill protein column chromatography fillers and protein solutions to chromatograph the target protein in the protein solution. The detection liquid injection tube passes through the wall thickness direction of the tank body and is connected to the second cavity. The detection liquid injection tube is used to inject the detection liquid into the second cavity. The detection liquid is used to combine with the target protein in the protein solution in the second cavity and mark the target protein to show the separation effect of the target protein in the second cavity, and the separation effect of the target protein in the second cavity reflects the separation effect of the target protein in the first cavity. Under the premise of not affecting the target protein in the first cavity, the separation condition of the target protein in the first cavity can be reflected by the chromatography effect of the target protein in the second cavity and the distribution of the chromatographically obtained protein along the height direction, thereby being able to observe the separation and purification effect of the target protein in real time and improving the convenience of knowing the separation and purification effect of the target protein during the chromatography process.

[0016] Other features and advantages of the present application will become apparent from the following detailed description of exemplary embodiments of the present application with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0018] Figure 1 This is an overall schematic diagram of a protein chromatography device according to some embodiments of the present application.

[0019] Figure 2 This is a schematic bottom view of a protein chromatography device according to some embodiments of the present application.

[0020] Figure 3 for Figure 1 A partial enlarged view of the detection liquid injection tube. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means intended to limit the present application and its application or use. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0022] Unless otherwise specifically stated, the relative arrangement, numerical expressions and numerical values ​​of the parts and steps set forth in these embodiments do not limit the scope of the application. Meanwhile, it should be understood that, for ease of description, the sizes of the various parts shown in the accompanying drawings are not drawn according to actual proportional relationships. The technology, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be considered as a part of the specification. In all examples shown and discussed here, any specific value should be interpreted as being merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters represent similar items in the following drawings, and therefore, once a certain item is defined in an accompanying drawing, it does not need to be further discussed in subsequent drawings.

[0023] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" may include both "above" and "below". The device may also be positioned in other different ways, and the spatially relative descriptions used here are interpreted accordingly.

[0024] refer to Figure 1 and 2 Some embodiments of the present application provide a protein chromatography device, including a chromatography tank 1 and a detection liquid injection tube 2. The chromatography tank 1 includes a tank body 11, a first cavity 12 and a second cavity 13 arranged in the inner cavity of the tank body 11. The first cavity 12 and the second cavity 13 are not connected to each other, and the bottom ends of the first cavity 12 and the second cavity 13 along the height direction Z of the tank body 11 are flush. The first cavity 12 and the second cavity 13 are both used to fill protein column chromatography fillers and protein solutions to chromatograph the target protein in the protein solution. The detection liquid injection tube 2 penetrates along the wall thickness direction of the tank body 11 and is connected to the second cavity 13. The detection liquid injection tube 2 is used to inject the detection liquid into the second cavity 13. The detection liquid is used to combine with the target protein in the protein solution in the second cavity 13 and mark the target protein to show the separation effect of the target protein in the second cavity 13, and the separation effect of the target protein in the second cavity 13 reflects the separation effect of the target protein in the first cavity 12.

[0025] During operation, first, protein column chromatography fillers of the same height are injected into the first cavity 12 and the second cavity 13 respectively, and protein solutions of the same height are added into the first cavity 12 and the second cavity 13 respectively, and then the detection liquid is injected into the second cavity 13 through the detection liquid injection tube 2, so that the detection liquid is combined with the target protein in the protein solution and marked. Based on this, the staff can more clearly know the chromatography effect of the target protein in the second cavity 13 and the distribution of the chromatographic target protein in the second cavity 13 along the height direction Z.

[0026] Since the liquid levels in the first cavity 12 and the second cavity 13 are equal, and the column chromatography filler and the protein solution are also the same, the separation of the target protein in the first cavity 12 can be reflected by the chromatography effect of the target protein in the second cavity 13 and the distribution of the chromatographic target protein along the height direction Z without affecting the target protein in the first cavity 12, so that the separation and purification effect of the target protein can be observed in real time, which improves the convenience of obtaining the separation and purification effect of the target protein during the chromatography process.

[0027] In some embodiments, the top ends of the first cavity 12 and the second cavity 13 along the height direction Z are open, so as to facilitate filling the first cavity 12 and the second cavity 13 with protein column chromatography fillers and protein solutions.

[0028] In some embodiments, the volume of the first cavity 12 is greater than the volume of the second cavity 13. Specifically, the axis of the tank body 11 extends along the height direction Z, the axes of the first cavity 12 and the second cavity 13 are parallel to the axis of the tank body 11, and the first cavity 12 is used to separate and purify the target protein in the protein solution. The second cavity 13 mainly serves as a control, so that the staff can know the separation effect of the target protein in the second cavity 13, and then know the separation effect of the target protein in the first cavity 12. Therefore, making the volume of the first cavity 12 larger can make a smaller part of the inner cavity of the tank body 11 Used to reflect the separation and purification of the target protein, and make a larger part of the inner cavity of the tank body 11 Used to perform chromatographic separation on the target protein, ensuring the ability of the protein chromatography device to separate proteins by chromatography.

[0029] In some embodiments, the protein chromatography device further comprises a partition plate 9. The partition plate 9 divides the inner cavity of the tank body 11 into two regions in the cross section of the tank body 11 to form a first cavity 12 and a second cavity 13 respectively.

[0030] Specifically, the top ends of the first cavity 12 and the second cavity 13 along the height direction are flush, in other words, the first cavity 12 and the second cavity 13 have the same height, the partition plate 9 is constructed as a long plate, the length direction of the partition plate 9 is parallel to the axial direction of the tank body 11, and the length of the partition plate 9 is the same as the height of the first cavity 12 and the second cavity 13, and the two long sides of the partition plate 9 are connected to the cavity wall of the inner cavity of the tank body 11, so that the tank body 11 is partitioned by the partition plate 9 in the entire height direction Z.

[0031] In some embodiments, the protein chromatography device further comprises a discharge device 3. The discharge device 3 is connected to the bottom of the first cavity 12 and the second cavity 13 along the height direction Z. The discharge device 3 is used to discharge the sample liquid formed by the protein solution in the first cavity 12 and the second cavity 13 after chromatography. The discharge device 3 can discharge the sample liquid in the first cavity 12 and the second cavity 13 to collect the separated protein.

[0032] In order to improve the smoothness of the sample liquid discharge process, in some embodiments, the protein chromatography device further includes a decompression device 4. The decompression device 4 is connected to the first cavity 12 and the second cavity 13. The decompression device 4 is used to reduce the pressure of the solution in the first cavity 12 and the second cavity 13 to promote the sample liquid to be discharged through the discharge device 3.

[0033] In some embodiments, the decompression device 4 includes a decompression pump 41. The decompression pump 41 is connected to the first cavity 12 and the second cavity 13. The decompression pump 4 is used to extract part of the sample liquid in the first cavity 12 and the second cavity 13 to reduce the pressure of the solution in the first cavity 12 and the second cavity 13. Specifically, the decompression pump 41 is connected to the first cavity 12 and the second cavity 13 through two hoses 42, respectively, so that the decompression pump 41 can simultaneously suck the sample liquid in the first cavity 12 and the second cavity 13.

[0034] In some embodiments, the reduced pressure pump 41 comprises a peristaltic pump.

[0035] In some embodiments, the discharge device 3 includes a first discharge pipe 31 and a second discharge pipe 32. The bottom end of the first cavity 12 along the height direction Z is connected to the first discharge pipe 31. The bottom end of the second cavity 13 along the height direction Z is connected to the second discharge pipe 32. The first discharge pipe 31 and the second discharge pipe 32 are used to discharge the first cavity 12 and the second cavity 13, respectively.

[0036] In some embodiments, the protein chromatography device further comprises a heat-insulating sleeve 5. The heat-insulating sleeve 5 is sleeved on the outer side of the tank body 11. There is a gap between the heat-insulating sleeve 5 and the circumferential surface of the tank body 11. The gap is used to fill a heat-conducting medium to heat or cool the tank body 11.

[0037] Specifically, by providing the heat-insulating sleeve 5, the tank body 11 can be surrounded by a heat-conducting medium that can be used to regulate the temperature of the tank body 11. The temperature of the tank body 11 is adjusted by regulating the temperature of the heat-conducting medium. Therefore, when chromatography of different proteins is performed, the temperature of the tank body 11 is adaptively adjusted to ensure the high efficiency of protein separation by the protein chromatography device.

[0038] In some embodiments, the heat-conducting medium may be water, and the heat-insulating sleeve 5 is made of a transparent material, so that the staff can intuitively know the effect of protein separation and purification in the second cavity 13 .

[0039] In some embodiments, the profiles of the tank body 11 and the insulation sleeve 5 are both constructed as cylinders. The top ends of the tank body 11 and the insulation sleeve 5 along the height direction Z are roughly flush. The bottom end of the insulation sleeve 5 along the height direction Z is higher than the bottom end of the tank body 11 along the height direction Z. In other words, the bottom end of the tank body 11 is located outside the insulation sleeve 5, leaving space for the arrangement of the discharge device 3 and the pressure reducing device 4.

[0040] In order to facilitate the control of the temperature of the heat-conducting medium, in some embodiments, the protein chromatography device further comprises a temperature control device 6. The temperature control device 6 is connected to the heat-insulating sleeve 5. The temperature control device 6 is used to adjust the temperature of the heat-conducting medium to increase or decrease.

[0041] Specifically, the protein chromatography device also includes a water inlet pipe 10, one end of which is connected to the bottom of the insulation sleeve 5, and the other end of the water inlet pipe 10 is connected to a water source. Water can be injected into the insulation sleeve 5 through the water inlet pipe 10 to adjust the water content in the insulation sleeve 5, and the temperature control device 6 is arranged on the water inlet pipe 10, so as to complete the temperature adjustment of the water in the process of water passing through the temperature control device 6 and entering the insulation sleeve 5.

[0042] In some embodiments, the protein chromatography device further comprises a temperature detector 7. The temperature detector 7 is disposed on the heat-insulating sleeve 5. The temperature detector 7 is configured to detect and display the temperature of the heat-conducting medium in real time.

[0043] In some embodiments, the temperature detector 7 comprises a thermometer.

[0044] In some embodiments, at least a portion of the detection liquid injection tube 2 passes through the heat preservation sleeve 5 and extends to the radially outer side of the heat preservation sleeve 5. That is, the first end of the detection liquid injection tube 2 is connected to the second cavity 13, and the second end of the detection liquid injection tube 2 is located outside the heat preservation sleeve 5, and the detection liquid can be injected into the second cavity 13 through the second end of the detection liquid injection tube 2.

[0045] refer to Figure 3 In some embodiments, a sealing plug 8 is provided in the detection liquid injection tube 2. The sealing plug 8 is used to seal the second end of the detection liquid injection tube 2 to reduce pollutants in the environment from entering the second cavity 13. When injecting the detection liquid into the second cavity 13, a syringe can be used to insert the sealing plug 8 to complete the injection.

[0046] In some embodiments, the protein chromatography device includes a plurality of detection liquid injection tubes 2, and the plurality of detection liquid injection tubes 2 are arranged at intervals along the height direction Z.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present application rather than to limit it. Although the present application has been described in detail with reference to the preferred embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the present application can still be modified or some technical features can be replaced by equivalents without departing from the spirit of the technical solution of the present application, which should be included in the scope of the technical solution for protection in the present application.

Claims

1. A protein chromatography device, characterized in that: include: A chromatography tank (1), comprising a tank body (11), a first cavity (12) and a second cavity (13) arranged in the inner cavity of the tank body (11), wherein the first cavity (12) and the second cavity (13) are not connected to each other, and the bottom ends of the first cavity (12) and the second cavity (13) along the height direction (Z) of the tank body (11) are flush, and the first cavity (12) and the second cavity (13) are both used to fill protein column chromatography filler and protein solution to chromatograph the target protein in the protein solution; and A detection liquid injection tube (2) is passed through the wall thickness direction of the tank body (11) and is connected to the second cavity (13). The detection liquid injection tube (2) is used to inject detection liquid into the second cavity (13). The detection liquid is used to combine with the target protein in the protein solution in the second cavity (13) and label the target protein to show the separation effect of the target protein in the second cavity (13). The separation effect of the target protein in the second cavity (13) reflects the separation effect of the target protein in the first cavity (12).

2. The protein chromatography device according to claim 1, characterized in that: The volume of the first cavity (12) is greater than the volume of the second cavity (13).

3. The protein chromatography device according to claim 1, characterized in that: The protein chromatography device further comprises a partition plate (9), wherein the partition plate (9) divides the inner cavity of the tank body (11) into two regions on a cross section of the tank body (11) to respectively form the first cavity (12) and the second cavity (13).

4. The protein chromatography device according to claim 1, characterized in that: The protein chromatography device further comprises a discharge device (3), the discharge device (3) being connected to the bottom of the first cavity (12) and the second cavity (13) along the height direction (Z), and the discharge device (3) being used to discharge sample liquid formed by the protein solution in the first cavity (12) and the second cavity (13) after chromatography.

5. The protein chromatography device according to claim 4, characterized in that: The protein chromatography device further comprises a decompression device (4), wherein the decompression device (4) is connected to the first cavity (12) and the second cavity (13), and the decompression device (4) is used to reduce the pressure of the solution in the first cavity (12) and the second cavity (13) so as to cause the sample liquid to be discharged through the discharge device (3).

6. The protein chromatography device according to claim 5, characterized in that: The decompression device (4) comprises a decompression pump (41), the decompression pump (41) being connected to the first cavity (12) and the second cavity (13), the decompression pump (41) being used to extract part of the sample liquid in the first cavity (12) and the second cavity (13), so as to reduce the pressure of the solution in the first cavity (12) and the second cavity (13).

7. The protein chromatography device according to claim 4, characterized in that: The discharge device (3) comprises a first discharge pipe (31) and a second discharge pipe (32); the bottom end of the first cavity (12) along the height direction (Z) is connected to the first discharge pipe (31), and the bottom end of the second cavity (13) along the height direction (Z) is connected to the second discharge pipe (32).

8. The protein chromatography device according to any one of claims 1 to 7, characterized in that: The protein chromatography device further comprises a heat-insulating sleeve (5), wherein the heat-insulating sleeve (5) is sleeved on the outside of the tank body (11), and a gap exists between the heat-insulating sleeve (5) and the circumferential surface of the tank body (11), wherein the gap is used to fill a heat-conducting medium to heat or cool the tank body (11).

9. The protein chromatography device according to claim 8, characterized in that: It also comprises a temperature control device (6), the temperature control device (6) being connected to the heat-insulating sleeve (5), and the temperature control device (6) being used to adjust the temperature of the heat-conducting medium to increase or decrease.

10. The protein chromatography device according to claim 8, characterized in that: It also comprises a temperature detector (7), wherein the temperature detector (7) is arranged on the heat-insulating sleeve (5), and the temperature detector (7) is configured to detect and display the temperature of the heat-conducting medium in real time.

11. The protein chromatography device according to claim 8, characterized in that: At least a portion of the detection liquid injection tube (2) passes through the heat-insulating sleeve (5) and extends to the radially outer side of the heat-insulating sleeve (5).

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