96-well plate containing molecular screening
By designing a combined structure of 96-well plate, including a 96-well plate body, screening laminate, screening tube and molecular screening network, the problem that existing 96-well plates cannot be directly screened is solved, and rapid and accurate molecular screening is achieved, and experimental efficiency is improved.
Patent Information
- Application Number
- CN202421564257.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-04
- Publication Date
- 2025-04-18
- Estimated Expiration
- 2034-07-04
AI Technical Summary
The existing 96-well plate has a simple structure and only has sample storage function. It is impossible to directly perform molecular screening, which affects the experimental efficiency and accuracy.
A 96-well plate with a combined structure is designed, including a 96-well plate body, a removable screening laminate, a screening tube and a molecular screening net. The experimental liquid is pushed through the molecular screening net in the screening tube through the piston to achieve molecular screening.
This design realizes fast and accurate molecular screening, saves screening time, improves experimental efficiency, and facilitates cleaning and maintenance through a combined structure.
Smart Images
Figure CN222766356U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of 96-well plates, in particular to a 96-well plate containing molecular screening. Background Art
[0002] The 96-well plate is a standard experimental tool widely used in biological, chemical and medical research. It is made of plastic and consists of a rectangular plate and 96 wells arranged in 8 rows and 12 columns, each of which can hold a small amount of liquid sample. The 96-well plate is commonly used in experiments such as high-throughput screening, ELISA, PCR reactions and cell culture. Its standard size is 127.76 mm × 85.48 mm, the hole spacing is 9 mm, the capacity of each well is usually between 100 and 300 microliters, and the material is mostly polystyrene or polypropylene to ensure chemical compatibility and durability. 96-well plates of different colors and transparencies can be used for different types of optical measurements, greatly improving experimental efficiency and accuracy.
[0003] The current 96-well plate has a relatively simple structure and only has the function of sample storage. After molecular screening, the appropriate sample needs to be pipetted into the well. This process often requires the use of other equipment, which may result in missing the best time for pipetting, affecting experimental efficiency and accuracy. Therefore, designing a 96-well plate that can directly perform molecular screening is very necessary for experimental research. Utility Model Content
[0004] In order to solve the above problems, the utility model proposes a combined structure, which is easy to disassemble, saves molecular screening time, and directly injects experimental liquid into the wells to form a 96-well plate containing molecular screening.
[0005] In order to solve the above-mentioned technical problems, the technical solution proposed by the utility model is: a 96-well plate containing molecular screening, including a 96-well plate body, a detachable screening layer plate is provided above the 96-well plate body, a plurality of screening tubes are provided on the screening layer plate, a molecular screening net is provided in the screening tubes, the screening tubes correspond one-to-one to the holes on the 96-well plate body and the lower ends of the screening tubes are arranged in the holes on the 96-well plate body, a detachable pressing plate is provided above the screening layer plate, a plurality of pressing rods are connected to the lower end of the pressing plate, a piston is connected to the lower end of the pressing rod, the piston corresponds one-to-one to the screening tubes and is adapted to the screening tubes.
[0006] Furthermore, positioning rods are connected at the four corners below the screening layer plate, and positioning holes matching the positioning rods are provided at the four corners of the 96-well plate body, and the positioning rods are inserted into the positioning holes.
[0007] Based on the above features, the positioning rod and the positioning hole can not only stably fix the screening layer plate on the 96-well plate body, but also facilitate fast and accurate positioning and installation.
[0008] Furthermore, insert pipes are symmetrically connected on both sides above the screening layer plate, and insert rods matching the insert pipes are symmetrically connected on both sides below the pressure plate, and the insert rods are inserted into the insert pipes.
[0009] Based on the above characteristics, the insertion rod and the insertion tube can quickly and accurately and stably install the pressure plate above the screening layer, which is convenient and practical.
[0010] Furthermore, grooves are symmetrically provided on both sides above the pressure plate, and a plurality of anti-slip protrusions are provided in the grooves.
[0011] Based on the above features, it is convenient to press the pressure plate stably.
[0012] Furthermore, the holes on the 96-well plate body, the screening tube, and the center of the piston are on the same vertical line.
[0013] Based on the above characteristics, it can be ensured that the piston enters the screening tube smoothly and stably, and the screening tube accurately enters the hole on the 96-well plate body, thereby ensuring a smooth molecular screening process.
[0014] Furthermore, a base is connected below the 96-well plate body.
[0015] Based on the above characteristics, the stability and hygiene of the 96-well plate body can be guaranteed.
[0016] Compared with the prior art, the utility model has the following advantages: the 96-well plate body, the screening layer plate and the pressing plate can be a combined structure with each other, and through the cooperation of the positioning rod and the positioning hole, the insertion tube and the insertion rod, not only is it convenient to quickly and accurately position and install, but also it can be quickly disassembled, which is convenient for cleaning and maintenance. The screening tube is directly inserted into the hole on the 96-well plate body, and the experimental liquid can be accurately injected into the hole, which effectively avoids waste. At the same time, the piston can push the experimental liquid to move downward and smoothly pass through the molecular screening net in the screening tube, thereby improving the screening speed and saving screening time. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 It is a three-dimensional diagram of the utility model;
[0018] Figure 2 It is the front view of the utility model;
[0019] Figure 3 It is a stereoscopic diagram of the main body of the 96-well plate of the utility model;
[0020] Figure 4 It is a top view of the utility model after the screening layer plate and the 96-well plate body are assembled.
[0021] As shown in the figure: 1. 96-well plate body; 2. Screening layer plate; 3. Screening tube; 4. Molecular screening net; 5. Pressing plate; 6. Pressing rod; 7. Piston; 8. Positioning rod; 9. Positioning hole; 10. Insertion tube; 11. Insertion rod; 12. Base. DETAILED DESCRIPTION
[0022] The utility model is further described in detail below in conjunction with the accompanying drawings.
[0023] Combined with Figure 2 , Attachment Figure 3 A 96-well plate for molecular screening includes a 96-well plate body 1, and a base 12 is connected below the 96-well plate body 1 to ensure the stability and hygiene of the 96-well plate body 1.
[0024] Combined with Figure 1 , Attachment Figure 2 , Attachment Figure 3 A detachable screening layer plate 2 is provided above the 96-well plate body 1, and positioning rods 8 are connected at the four corners below the screening layer plate 2. Positioning holes 9 matching the positioning rods 8 are provided at the four corners of the 96-well plate body 1. The positioning rods 8 are inserted into the positioning holes 9. The positioning rods 8 cooperate with the positioning holes 9 to stably fix the screening layer plate 2 on the 96-well plate body 1, and facilitate quick and accurate positioning and installation.
[0025] Combined with Figure 1 , Attachment Figure 2 , Attachment Figure 4 A plurality of screening tubes 3 are arranged on the screening layer 2, and a molecular screening net 4 is arranged in the screening tubes 3. The screening tubes 3 correspond to the holes on the 96-well plate body 1 one by one, and the lower ends of the screening tubes 3 are arranged in the holes on the 96-well plate body 1. A detachable pressing plate 5 is arranged above the screening layer 2, and plugging tubes 10 are symmetrically connected on both sides above the screening layer 2, and plugging rods 11 matching the plugging tubes 10 are symmetrically connected on both sides below the pressing plate 5. The plugging rods 11 are inserted in the plugging tubes 10, and the plugging rods 11 cooperate with the plugging tubes 10 to quickly and accurately and stably install the pressing plate 5 above the screening layer 2, which is convenient and practical.
[0026] Combined with Figure 1 Grooves are symmetrically arranged on both sides above the pressing plate 5 , and a plurality of anti-slip protrusions are arranged in the grooves to facilitate stable pressing of the pressing plate 5 .
[0027] Combined with Figure 1 , Attachment Figure 2A plurality of pressing rods 6 are connected to the lower side of the pressing plate 5, and a piston 7 is connected to the lower end of the pressing rod 6. The piston 7 corresponds to and matches the screening tube 3 one by one. The holes on the 96-well plate body 1, the screening tube 3, and the piston 7 are located on the same vertical line, which can ensure that the piston 7 can smoothly and stably enter the screening tube 3, and the screening tube 3 can accurately enter the holes on the 96-well plate body 1, thereby ensuring a smooth and smooth molecular screening process.
[0028] The specific implementation mode of the utility model is as follows: the 96-well plate body 1 is stably placed in a suitable position, the screening layer plate 2 is installed above the 96-well plate body 1, and the positioning rod 8 is inserted into the positioning hole 9. At this time, the screening tubes 3 are aligned one by one with the holes on the 96-well plate body 1 and inserted into the holes, and experimental liquid is added to each screening tube 3. The pressing plate 5 is installed above the screening layer plate 2, and the insertion rod 11 is inserted into the insertion tube 10. At this time, the piston 7 is aligned one by one with the screening tube 3 and is located above the screening tube 3. The pressing plate 5 is pressed downward, and the pressing plate 5 drives the piston 7 to enter the screening tube 3 through the pressing rod 6. The piston 7 can push the experimental liquid downward, so that it can quickly pass through the molecular screening net 4 for screening, and the screened experimental liquid enters the holes of the 96-well plate body 1.
[0029] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the term "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0030] The above description of the utility model and its implementation methods is not restrictive. The drawings show only one implementation method of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.
Claims
1. A 96-well plate for molecular screening, comprising a 96-well plate body (1), characterized in that: A detachable screening layer plate (2) is provided above the 96-well plate body (1), a plurality of screening tubes (3) are provided on the screening layer plate (2), a molecular screening net (4) is provided in the screening tubes (3), the screening tubes (3) correspond one-to-one with the holes on the 96-well plate body (1), and the lower ends of the screening tubes (3) are arranged in the holes on the 96-well plate body (1), a detachable pressing plate (5) is provided above the screening layer plate (2), a plurality of pressing rods (6) are connected to the lower end of the pressing rod (6), a piston (7) is connected to the lower end of the piston (7), and the piston (7) corresponds one-to-one with the screening tubes (3) and is adapted to the screening tubes (3).
2. A 96-well plate containing molecular screening according to claim 1, characterized in that: Positioning rods (8) are connected at the four corners below the screening layer plate (2), and positioning holes (9) matching the positioning rods (8) are provided at the four corners of the 96-well plate body (1), and the positioning rods (8) are inserted into the positioning holes (9).
3. A 96-well plate containing molecular screening according to claim 1, characterized in that: Insertion tubes (10) are symmetrically connected on both sides above the screening layer plate (2), and insertion rods (11) matching the insertion tubes (10) are symmetrically connected on both sides below the pressing plate (5), and the insertion rods (11) are inserted into the insertion tubes (10).
4. A 96-well plate containing molecular screening according to claim 1, characterized in that: Grooves are symmetrically arranged on both sides of the upper side of the pressing plate (5), and a plurality of anti-slip protrusions are arranged in the grooves.
5. A 96-well plate containing molecular screening according to claim 1, characterized in that: The centers of the holes on the 96-well plate body (1), the screening tube (3) and the piston (7) are on the same vertical line.
6. A 96-well plate containing molecular screening according to claim 1, characterized in that: A base (12) is connected to the lower side of the 96-well plate body (1).