Multi-temperature-zone PCR centrifugal tube box

Abstract

The utility model discloses a multi-temperature-zone PCR (Polymerase Chain Reaction) centrifuge tube box which comprises a bottom plate, a plurality of first tube boxes surrounded by first heat preservation boxes and a plurality of second tube boxes surrounded by second heat preservation boxes, the first tube boxes and the second tube boxes are positioned at the upper part of the bottom plate, and the first heat preservation boxes and the second heat preservation boxes are of structures which are hollow at the lower parts and are communicated with the first tube boxes and the second tube boxes; a first groove communicated with the first heat preservation box and a second groove communicated with the second heat preservation box are formed in the bottom of the bottom plate, an electric heating piece and a refrigerant plate are arranged in the first groove and the second groove respectively, a temperature adjusting component extending into the first groove and the second groove is arranged on one side of the bottom plate, and the air flow speed in the first groove and the second groove can be adjusted conveniently. According to the utility model, the first heat preservation box and the second heat preservation box can be respectively heated or refrigerated for heat preservation, after a long time, the temperature in the boxes is still stable, the stability of a PCR experiment is improved, the category and various codes of centrifugal tubes in the boxes can be recorded, the situation that reagents are missed and mistakenly added in the experiment process is prevented, and the experiment accuracy is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of PCR centrifugal tube boxes, in particular to a multi-temperature zone PCR centrifugal tube box. Background Art

[0002] During the PCR amplification operation, some commonly used PCR reaction reagents are involved. The requirements for the storage temperature of these reagents during the experimental operation are different. For example, the EP tubes (microcentrifuge tubes) containing enzymes are generally placed on an ice box, while other thawed reagents are placed on an ordinary EP tube rack at normal temperature to prevent these reagents from freezing again at low temperatures. PCR sample addition operations often need to be performed on ice, which requires the simultaneous use of multiple EP tube racks with different temperatures. Multiple EP tube racks with different temperatures are not easy to operate during use, and it is also easy to leak or add reagents by mistake, affecting the normal progress of the experiment. Therefore, some centrifuge tube boxes that combine multiple EP tube racks have appeared on the market.

[0003] For example, the existing patent document with publication number CN215708097U discloses "a multi-temperature zone integrated PCR centrifuge tube box", which includes a box rack, and the inside of the box rack is respectively installed with an experimental box, a low-temperature box and a normal temperature box, and the upper surfaces of the low-temperature box and the normal temperature box are evenly spaced with tube holes, and the lower surface of the box rack is installed with a fixed component, and the outer surfaces of the experimental box, the low-temperature box and the normal temperature box are all installed with a heat preservation component, which insulates the inside of the box body, thereby avoiding mutual interference between different boxes. However, this solution only insulates the inside of the box body, and after a long time, the temperature inside the box will still be lost, which is not conducive to the PCR experiment operation; and this solution may still cause reagents to be missed or added by mistake. Utility Model Content

[0004] In order to solve the technical problems in the prior art that only the inside of the box body is kept warm, the temperature inside the box will still be lost after a long time, which is not conducive to the PCR experiment operation, and reagents may be missed or added incorrectly, the utility model provides the following technical solutions.

[0005] The utility model discloses a multi-temperature zone PCR centrifuge tube box, comprising a bottom plate and a plurality of first tube boxes surrounded by a first heat-insulating box and a plurality of second tube boxes surrounded by a second heat-insulating box located on the upper portion of the bottom plate, the first heat-insulating box and the second heat-insulating box being both hollow and connected to the first tube box and the second tube box, a first groove connected to the first heat-insulating box and a second groove connected to the second heat-insulating box being provided at the bottom of the bottom plate, a heating plate and a refrigerant plate being provided in the first groove and the second groove respectively, a temperature regulating component extending into the first groove and the second groove being provided on one side of the bottom plate to facilitate adjusting the air flow rate in the first groove and the second groove.

[0006] As a further technical solution, the temperature regulating component includes a fan fixed on the upper part of the fixed block on one side of the bottom plate and a ventilation pipe connected to the fan, and the ventilation pipe is connected to the first groove and the second groove through a plurality of shunt pipes.

[0007] As a further technical solution, check valves are provided at the connecting points between the shunt pipe and the first groove and the second groove.

[0008] As a further technical solution, one side of each of the first insulation box and the second insulation box is provided with a label area, and the label area is used to record the type of centrifuge tube and various codes.

[0009] As a further technical solution, a control screen is provided on one side of the upper portion of the base plate.

[0010] As a further technical solution, the bottom plate, the first insulation box and the second insulation box are all made of heat insulating materials.

[0011] The beneficial effects of the utility model are as follows: the utility model is provided with a plurality of first grooves and second grooves at the bottom of the base plate, and the first grooves and the second grooves are respectively provided with electric heating plates and refrigerant plates, which are respectively used to heat or cool the first insulation box and the second insulation box. After a long time, the temperature in the box remains stable, thereby improving the stability of the PCR experiment. A temperature regulating component is provided on one side of the bottom, and the wind blown by the fan can accelerate the air circulation in the first groove and the second groove, and prevent the air above the first tube box and the second tube box from affecting the average temperature in the box, thereby improving the accuracy of temperature control. A label area is provided on one side of the first insulation box and the second insulation box, and the label area can be used to record the type of centrifuge tube and various codes, thereby preventing the situation of reagent leakage or mistaken addition during the experiment, thereby improving the accuracy of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is a schematic diagram of the structure of the multi-temperature zone PCR centrifuge tube box of the utility model;

[0013] Figure 2 This is a schematic diagram of the bottom plate structure of the multi-temperature zone PCR centrifuge tube box of the utility model;

[0014] Figure 3 This is a schematic diagram of the first insulation box of the multi-temperature zone PCR centrifuge tube box of the utility model;

[0015] In the figure: 1-bottom plate; 101-fixed block; 102-first groove; 103-second groove; 2-first insulation box; 3-first pipe box; 4-second insulation box; 5-second pipe box; 6-label area; 7-control panel; 8-temperature adjustment component 801-fan; 802-ventilation pipe; 803-shunt pipe; 9-electric heating plate; 10-refrigerant plate. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the utility model and are not used to limit the utility model. It should be noted that the embodiments in the utility model and the features in the embodiments can be combined with each other without conflict.

[0017] In the description of the present invention, it should be understood that the terms "upper", "lower" and "both sides" are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second" and the like are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features.

[0018] like Figure 1 , Figure 2 and Figure 3 As shown, the utility model is a multi-temperature zone PCR centrifugal tube box, comprising a bottom plate 1 and a plurality of first tube boxes 3 surrounded by a first insulation box 2 and a plurality of second tube boxes 5 surrounded by a second insulation box 4 located on the upper part of the bottom plate 1. The bottom plate 1, the first insulation box 2 and the second insulation box 4 are all made of heat insulation materials, such as polyurethane, polyethylene foam and the like. In order to improve the stable support of the bottom plate 1 to the above-mentioned box body and the centrifugal tube, a layer of hard plastic or a layer of metal plate can also be set at the bottom of the bottom plate 1.

[0019] In a preferred embodiment, the first insulation box 2 and the second insulation box 4 are both hollow structures, and the first insulation box 2 and the second insulation box 4 are connected to the first tube box 3 and the second tube box 5, respectively. That is, the first tube box 3 and the second tube box 5 are respectively bonded to the inner wall of the first insulation box 2 and the second insulation box 4. After the centrifuge tube is placed in the first tube box 3 and the second tube box 5, the lower part of the centrifuge tube is exposed in the first insulation box 2 and the second insulation box 4, so that the first insulation box 2 and the second insulation box 4 can continuously increase the appropriate temperature for the centrifuge tube while keeping warm. According to actual experimental requirements, the first tube box 3 and the second tube box 5 can be set to a normal temperature zone and a low temperature zone, respectively. The size of the first tube box 3 and the second tube box 5 shown in the figure is not limited. In order to match the aperture size of the centrifuge tube, it can be set to a variety of different sizes. The first insulation box 2 and the second insulation box 4 are both provided with a label area 6 on one side, and the label area 6 is used to record the type of the centrifuge tube and various codes to prevent the situation of reagent leakage and mis-addition during the experiment.

[0020] In a preferred embodiment, the bottom of the bottom plate 1 is provided with a plurality of first grooves 102 communicating with the first heat preservation box 2 and second grooves 103 communicating with the second heat preservation box 4. In this embodiment, the first tube box 3 is a normal temperature zone, the second tube box 5 is a low temperature zone, the first groove 102 and the second groove 103 are respectively provided with electric heating plates 9 and refrigerant plates 10, the electric heating plates 9 and refrigerant plates 10 are connected to an external power source and a cold source, and a control panel 7 is provided on one side of the upper part of the bottom plate 1. The control panel 7 is connected to an existing control system for controlling the external power source and the cold source so that the electric heating plates 9 and the refrigerant plates 10 reach the corresponding temperature.

[0021] In a preferred embodiment, a temperature regulating component 8 extending into the first groove 102 and the second groove 103 is provided on one side of the bottom plate 1, so as to adjust the air flow rate in the first groove 102 and the second groove 103. Specifically, a fixed block 101 is provided on one side of the bottom plate 1, and the temperature regulating component 8 includes a fan 801 fixed on the upper part of the fixed block 101 and a ventilation pipe 802 connected to the fan 801, and the ventilation pipe 802 is connected to the first groove 102 and the second groove 103 through a plurality of shunt pipes 803. A check valve is provided at the connection point between the shunt pipe 803 and the first groove 102 and the second groove 103. The wind force of the fan 1 flows into the plurality of first grooves 102 and the second groove 103 from the shunt pipe 803 after passing through the ventilation pipe 802, respectively, to promote the air circulation in the first insulation box 2 and the second insulation box 4, so that the temperature in the first tube box 3 and the second tube box 5 is constant, and the accuracy of the experiment is improved.

[0022] The preferred specific implementation modes and embodiments of the utility model are described in detail above in conjunction with the accompanying drawings, but the utility model is not limited to the above implementation modes and embodiments, and various changes or equivalent substitutions can be made within the knowledge scope of those skilled in the art without departing from the concept of the utility model. Therefore, the utility model is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application belong to the scope protected by the utility model.

Claims

1. A multi-temperature zone PCR centrifuge tube box, comprising a bottom plate (1) and a plurality of first tube boxes (3) surrounded by a first insulation box (2) and a plurality of second tube boxes (5) surrounded by a second insulation box (4) located on the upper part of the bottom plate (1), characterized in that: The first heat-insulating box (2) and the second heat-insulating box (4) are both hollow structures and are connected to the first tube box (3) and the second tube box (5); the bottom of the base plate (1) is provided with a first groove (102) connected to the first heat-insulating box (2) and a second groove (103) connected to the second heat-insulating box (4); the first groove (102) and the second groove (103) are respectively provided with an electric heating plate (9) and a refrigerant plate (10); one side of the base plate (1) is provided with a temperature regulating component (8) extending into the first groove (102) and the second groove (103) so as to adjust the air flow rate in the first groove (102) and the second groove (103).

2. The multi-temperature zone PCR centrifuge tube box according to claim 1, characterized in that: The temperature adjustment component (8) comprises a fan (801) fixed on the upper part of a fixed block (101) on one side of the bottom plate (1) and a ventilation pipe (802) connected to the fan (801); the ventilation pipe (802) is connected to the first groove (102) and the second groove (103) through a plurality of diverter pipes (803).

3. The multi-temperature zone PCR centrifuge tube box according to claim 2, characterized in that: Check valves are provided at the connecting points between the diverter pipe (803) and the first groove (102) and the second groove (103).

4. The multi-temperature zone PCR centrifuge tube box according to claim 1, characterized in that: The first heat preservation box (2) and the second heat preservation box (4) are both provided with a label area (6) on one side, and the label area (6) is used to record the type of centrifuge tube and various codes.

5. The multi-temperature zone PCR centrifuge tube box according to claim 1, characterized in that: A control panel (7) is provided on one side of the upper portion of the bottom plate (1).

6. The multi-temperature zone PCR centrifuge tube box according to claim 1, characterized in that: The bottom plate (1), the first heat-insulating box (2) and the second heat-insulating box (4) are all made of heat-insulating materials.

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