PCR liquid cooling device and PCR detection equipment

Abstract

The utility model discloses a PCR (polymerase chain reaction) liquid cooling device and PCR detection equipment, the PCR liquid cooling device comprises a temperature control base and a liquid cooling head, the temperature control base forms a plug-in mounting space for fitting and plug-in mounting of a PCR reaction tube, the liquid cooling head is sleeved on the peripheral wall of the plug-in mounting space, and the liquid cooling head and the peripheral wall enclose to form a liquid cooling flow channel; the two opposite sides of the liquid cooling head are provided with the liquid inlet and the liquid outlet which are communicated with the liquid cooling flow channel, cooling liquid can form forced convection cooling on the PCR reaction tube through the temperature control base, compared with air cooling, the cooling liquid makes direct contact with the temperature control base to rapidly cool the tube body, the cooling speed can be obviously increased, the cooling conversion time can be obviously shortened, and the cooling efficiency is improved. The purpose of improving the cooling efficiency is achieved.

Description

Technical Field

[0001] The utility model belongs to the technical field of molecular detection, and in particular relates to a PCR liquid cooling device and PCR detection equipment. Background Art

[0002] Polymerase Chain Reaction (PCR) is a biological technology that amplifies DNA fragments by artificially controlling external conditions. This technology is widely used in pathogen DNA detection and genetic detection of various organisms. PCR goes through three stages: deformation (95°C) - annealing (60°C) - extension (72°C) (in some cases, the annealing and extension stages can be combined) to complete the doubling of the DNA molecular chain. The PCR detection time is the total time of the above three stages and the temperature changes between each stage. Therefore, the key to shortening the PCR detection time is to speed up the temperature change speed and reduce the conversion time of each temperature range. In the current PCR detector of related technology, the well plate used to place the PCR reaction tube usually adopts air cooling, which has the defect of low cooling efficiency. Utility Model Content

[0003] In view of the above-mentioned defects or shortcomings, the utility model provides a PCR liquid cooling device and a PCR detection equipment, aiming to solve the technical problem that the existing PCR detector uses air cooling to reduce temperature, resulting in low cooling efficiency.

[0004] To achieve the above-mentioned purpose, the first aspect of the utility model provides a PCR liquid cooling device, wherein the PCR liquid cooling device includes a temperature control base and a liquid cooling head; the temperature control base forms an insertion space for PCR reaction tubes to be fitted and inserted; the liquid cooling head is sleeved on the outer peripheral wall of the insertion space and enclosed with the outer peripheral wall to form a liquid cooling channel, and a liquid inlet and a liquid outlet connected to the liquid cooling channel are provided on opposite sides of the liquid cooling head.

[0005] In an embodiment of the utility model, the temperature control base includes a temperature control bottom plate and a temperature control cylinder arranged on the temperature control bottom plate. The temperature control cylinder is enclosed on the temperature control bottom plate to form an insertion space extending in the vertical direction. The liquid cooling head cover is arranged on the outside of the temperature control cylinder and is enclosed with the temperature control cylinder to form a liquid cooling flow channel.

[0006] In an embodiment of the utility model, the liquid cooling head includes a flow channel portion, a liquid inlet portion and a liquid outlet portion. The flow channel portion is provided with a sleeve hole for the temperature control cylinder to pass through, and the flow channel portion is also provided with an annular groove on the outside of the sleeve hole. The inner wall of the annular groove and the outer peripheral wall of the temperature control cylinder are combined to form a liquid cooling flow channel. The liquid inlet portion and the liquid outlet portion are arranged on opposite sides of the flow channel portion and the liquid inlet and the liquid outlet are provided one by one.

[0007] In an embodiment of the utility model, a lap boss portion is protruding from the upper end of the flow channel portion, the lap boss portion is arranged around the sleeve hole, and the lap boss portion extends out of the flow channel portion in a direction toward the center line of the sleeve hole to form an extension surface that overlaps with the upper end surface of the temperature control cylinder.

[0008] In the embodiment of the utility model, a temperature sensor is provided on the temperature control cylinder at a position offset from the liquid cooling head.

[0009] In an embodiment of the utility model, a plurality of detection holes are sequentially spaced apart along the circumferential direction on the temperature control cylinder at positions staggered from the liquid cooling head.

[0010] In an embodiment of the utility model, the cross section of the insertion space is gradually reduced in the direction from the opening toward the bottom wall.

[0011] In the embodiment of the utility model, the temperature control base is configured as an integrally formed aluminum part.

[0012] In an embodiment of the utility model, the PCR liquid cooling device also includes a power component, a cooling box and a refrigeration assembly. The cooling box is used to store coolant. The refrigeration assembly is arranged on the cooling box. The power component is used to guide the coolant in the cooling box to the liquid inlet, and the liquid outlet flows back to the cooling box through the liquid outlet pipe.

[0013] In an embodiment of the utility model, the refrigeration assembly includes a semiconductor refrigeration sheet arranged on a cooling box, and a heat dissipation fan arranged on the semiconductor refrigeration sheet.

[0014] To achieve the above-mentioned purpose, the second aspect of the utility model provides a PCR detection device, wherein the PCR detection device includes a heating device and the PCR liquid cooling device described above, and the heating device is arranged on a temperature control base.

[0015] Through the above technical solution, the PCR liquid cooling device provided by the embodiment of the utility model has the following beneficial effects:

[0016] When using the above-mentioned PCR liquid cooling device, the PCR reaction tube can be placed in the insertion space of the temperature control base, and the coolant is introduced into the liquid inlet of the liquid cooling head. The coolant flows through the liquid cooling channel and flows out from the liquid outlet. Since the liquid cooling channel is formed by the liquid cooling head mounted on the outer peripheral wall of the insertion space and the outer peripheral wall of the temperature control base, the coolant flowing into the liquid cooling channel directly contacts the outer peripheral wall of the temperature control base, and the PCR reaction tube is fitly inserted in the insertion space, the coolant can form forced convection cooling for the PCR reaction tube through the temperature control base. Compared with air cooling, the tube body is quickly cooled down (the heat transfer resistance is zero, and the cooling rate is higher than 12°C / s) by direct contact between the coolant and the temperature control base, which can significantly increase the cooling rate, shorten the cooling conversion time, and achieve the purpose of improving the cooling efficiency.

[0017] Other features and advantages of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings are used to provide a further understanding of the embodiments of the present utility model and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the embodiments of the present utility model, but do not constitute a limitation on the embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without creative work. In the drawings:

[0019] Figure 1 It is a structural schematic diagram of a PCR liquid cooling device according to an embodiment of the utility model;

[0020] Figure 2 It is a schematic cross-sectional structure diagram of a liquid cooling head according to an embodiment of the utility model;

[0021] Figure 3 It is a structural schematic diagram of a PCR liquid cooling device and a heating device according to an embodiment of the utility model;

[0022] Figure 4 It is a disassembly schematic diagram of a PCR liquid cooling device and a heating device according to an embodiment of the utility model;

[0023] Figure 5 It is a partial structural schematic diagram of a PCR detection device according to one embodiment of the utility model.

[0024] Description of Reference Numerals

[0025] 100 Temperature control base 110 Temperature control base

[0026] 120 Temperature control cylinder 121 Insertion space

[0027] 122 detection hole 200 heating device

[0028] 210 Heating coil 220 Heating metal plate

[0029] 221 Threaded connector 230 Magnetic isolation sheet

[0030] 310 Liquid cooling head 311 Flow channel

[0031] 3111 Overlapping boss portion 3112 Epitaxial surface

[0032] 312 liquid inlet 313 liquid outlet

[0033] 314 Liquid cooling channel 315 Liquid inlet

[0034] 316 Liquid outlet 317 Set hole

[0035] 318 annular groove 320 power part

[0036] 330 Cooling box 340 Refrigeration components

[0037] 341 Semiconductor Refrigeration Chip 342 Cooling Fan

[0038] 350 Silicone hose 400 Temperature sensor DETAILED DESCRIPTION

[0039] The specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0040] The PCR liquid cooling device and PCR detection equipment of the present invention are described below with reference to the accompanying drawings.

[0041] like Figure 1 As shown, the utility model provides a PCR liquid cooling device, wherein the PCR liquid cooling device comprises:

[0042] The temperature control base 100 is formed with an insertion space 121 for the PCR reaction tube to be inserted and fitted;

[0043] The liquid cooling head 310 is sleeved on the outer peripheral wall of the insertion space 121 and encloses the outer peripheral wall to form a liquid cooling channel 314 . A liquid inlet 315 and a liquid outlet 316 communicating with the liquid cooling channel 314 are provided on opposite sides of the liquid cooling head 310 .

[0044] When the above-mentioned PCR liquid cooling device is used, the PCR reaction tube can be placed in the insertion space 121 of the temperature control base 100, and the coolant is introduced into the liquid inlet 315 of the liquid cooling head 310, and the coolant flows through the liquid cooling channel 314 and flows out from the liquid outlet 316. Since the liquid cooling channel 314 is formed by the liquid cooling head 310 which is sleeved on the outer peripheral wall of the insertion space 121 and the outer peripheral wall of the temperature control base 100, the coolant flowing into the liquid cooling channel 314 directly contacts the outer peripheral wall of the temperature control base 100, and the PCR reaction tube is inserted in the insertion space 121, the coolant can form forced convection cooling for the PCR reaction tube through the temperature control base 100. Compared with air cooling, the tube body is quickly cooled down (the heat transfer resistance is zero, and the cooling rate is higher than 12°C / s) by direct contact between the coolant and the temperature control base 100, which can obviously improve the cooling rate, shorten the cooling conversion time, and achieve the purpose of improving the cooling efficiency.

[0045] In the embodiment of the utility model, the temperature control base 100 includes a temperature control base plate 110 and a temperature control cylinder 120 disposed on the temperature control base plate 110. The temperature control cylinder 120 is enclosed on the temperature control base plate 110 to form an insertion space 121 extending in the vertical direction. The liquid cooling head 310 is sleeved on the outside of the temperature control cylinder 120 and enclosed with the temperature control cylinder 120 to form a liquid cooling channel 314. That is, the temperature control base plate 110 forms the bottom wall of the insertion space 121, the inside of the temperature control cylinder 120 forms the inner peripheral wall of the insertion space 121, and the outside of the temperature control cylinder 120 forms the outer peripheral wall of the insertion space 121. By constructing the temperature control cylinder 120 to form the insertion space 121, it is convenient to insert the PCR reaction tube inside and to realize the sleeve of the liquid cooling head 310 outside. Specifically, the periphery of the temperature control base plate 110 extends out of the temperature control cylinder 120 to increase the area of ​​the temperature control base plate 110, which is convenient for installing the heating device 200 on the side of the temperature control base plate 110 away from the temperature control cylinder 120. In addition, a connecting hole is provided at the position where the temperature control base 100 extends out of the temperature control cylinder 120 to facilitate detachable connection with the heating device 200 through a threaded connector 221 inserted into the connecting hole.

[0046] Furthermore, in order to ensure the sealing of the liquid cooling channel 314, a sealing structure can be provided at the joint between the liquid cooling head 310 and the temperature control cylinder 120, for example, a hot-melt sealant is coated, or a sealing groove for setting a sealing rubber ring is opened at the joint.

[0047] See also Figure 1 to Figure 2 In the embodiment of the utility model, the liquid cooling head 310 includes a flow channel portion 311, a liquid inlet portion 312 and a liquid outlet portion 313. The flow channel portion 311 is provided with a set hole 317 for the temperature control cylinder 120 to pass through, and the flow channel portion 311 is also provided with an annular groove 318 on the outside of the set hole 317. The inner wall of the annular groove 318 and the outer peripheral wall of the temperature control cylinder 120 are combined to form a liquid cooling flow channel 314. The liquid inlet portion 312 and the liquid outlet portion 313 are respectively arranged on opposite sides of the flow channel portion 311 and have liquid inlets 315 and liquid outlets 316 corresponding to each other. That is, the liquid cooling channel 314 is arranged in an annular shape, and the coolant can flow in from the liquid inlet 315 connected to one side of the liquid cooling channel 314, and flow out from the liquid outlet 316 connected to the other side of the liquid cooling channel 314, so that the liquid cooling head 310 is more in line with the flow field distribution, and the turbulence and turbulence of the fluid after passing through the temperature control cylinder 120 can be effectively reduced. Specifically, the temperature control cylinder 120 is set to be cylindrical, the liquid cooling channel 314 is set to be annular, and the outer diameter of the liquid cooling channel 314 is set to be twice the diameter of the temperature control cylinder 120, so that the best cooling effect can be achieved.

[0048] In the embodiment of the utility model, the upper end of the flow channel portion 311 is protruded with a lap boss portion 3111, which is arranged around the sleeve hole 317, and the lap boss portion 3111 extends from the flow channel portion 311 in the direction toward the center line of the sleeve hole 317 to form an epitaxial surface 3112 that overlaps with the upper end surface of the temperature control cylinder 120. That is, by adding the lap boss portion 3111, not only can the stability of the liquid cooling head 310 being sleeved on the temperature control cylinder 120 be ensured, but also the liquid cooling head 310 can be positioned and installed to avoid the phenomenon of inconsistent installation height in batch production.

[0049] like Figure 1 and Figure 3 As shown, in the embodiment of the utility model, the temperature control cylinder 120 is provided with a temperature sensor 400 at a position offset from the liquid cooling head 310. The additional temperature control sensor can facilitate real-time monitoring of the temperature of the temperature control cylinder 120, so as to control the coolant to pass into the liquid cooling channel 314 according to the real-time temperature. Specifically, the liquid cooling head 310 is sleeved on the upper area of ​​the temperature control cylinder 120, and the temperature sensor 400 can be arranged at the lower area of ​​the temperature control cylinder 120.

[0050] like Figure 1 and Figure 4 As shown, in the embodiment of the utility model, the temperature control cylinder 120 is provided with a plurality of detection holes 122 spaced in sequence along the circumferential direction at a position staggered from the liquid cooling head 310. The addition of a plurality of detection holes 122 on the temperature control cylinder 120 can facilitate the setting of the fluorescence detection optical path. Specifically, the liquid cooling head 310 is sleeved on the upper area of ​​the temperature control cylinder 120, and the plurality of detection holes 122 can be provided on the lower area of ​​the temperature control cylinder 120, and the plurality of detection holes 122 are evenly spaced around the circumference of the temperature control cylinder 120.

[0051] Please see again Figure 1 In the embodiment of the utility model, the cross section of the insertion space 121 is set to be tapered in the direction from the opening to the bottom wall. Specifically, the opening allows the PCR reaction tube to be inserted into the insertion space 121 from top to bottom, and because the outer side wall of the lower end of the PCR reaction tube is set to be inclined and extended inward in the direction from top to bottom, the cross section of the insertion space 121 is set to be tapered in the direction from the opening to the bottom wall, that is, the inner peripheral wall of the insertion space 121 is also set to be inclined and extended inward in the direction from top to bottom, so that not only can the PCR reaction tube and the inner peripheral wall of the insertion space 121 be laterally fitted, but also can play a guiding role in the insertion process of the PCR reaction tube, and ensure the insertion stability of the PCR reaction tube after the insertion is completed.

[0052] In the embodiment of the utility model, the temperature control base 100 is configured as an aluminum part that is integrally formed. That is, the temperature control base plate 110 and the temperature control cylinder 120 of the temperature control base 100 are integrally formed structures and can be configured as aluminum parts. Aluminum parts include aluminum alloy parts and pure aluminum parts. The temperature control base 100 is preferably a pure aluminum part, mainly because aluminum metal has extremely high thermal conductivity and low specific heat capacity, and plays an important role in rapid heating and cooling.

[0053] like Figure 1 and Figure 5 As shown, in the embodiment of the utility model, the PCR liquid cooling device also includes a power part 320, a cooling box 330 and a refrigeration assembly 340. The cooling box 330 is used to store the coolant. The refrigeration assembly 340 is arranged on the cooling box 330. The power part 320 is used to guide the coolant in the cooling box 330 to the liquid inlet 315, and the liquid outlet 316 flows back to the cooling box 330 through the liquid outlet pipe. That is, the coolant in the cooling box 330 can be cooled by the refrigeration assembly 340, and the power part 320, the liquid cooling head 310 and the cooling box 330 form a circulating flow loop of the coolant to realize the recycling of the coolant. Specifically, the coolant can be selected from a variety of heat-conducting media such as water, propylene glycol, heat-conducting oil, liquid metal, etc., but because water has the advantages of high thermal conductivity and environmental protection and easy availability, it is preferred to select pure water as the coolant to have a better cooling effect. More specifically, the power component 320 can be set as a water pump, and the power component 320 and the liquid inlet 315 of the liquid cooling head 310 as well as the cooling box 330 and the liquid outlet 316 can be connected through a silicone hose 350 , and a one-way valve can be provided on the silicone hose 350 .

[0054] In the embodiment of the utility model, the refrigeration assembly 340 includes a semiconductor refrigeration sheet 341 disposed on the cooling box 330, and a heat dissipation fan 342 disposed on the semiconductor refrigeration sheet 341. The semiconductor refrigeration sheet 341 is used to cool the coolant in the cooling box 330, and the heat dissipation fan 342 is used to dissipate heat from the semiconductor refrigeration sheet 341, thereby keeping the coolant in the cooling box 330 at a low temperature below 10°C, so as to achieve the best cooling effect, wherein -5°C is a better choice.

[0055] In addition, the utility model also provides a PCR detection device, wherein the PCR detection device includes a heating device 200 and the PCR liquid cooling device described above, and the heating device 200 is arranged on the temperature control base 100. Since the PCR detection device adopts all the technical solutions of the above embodiments, it has at least all the beneficial effects brought by the technical solutions of the above embodiments, which will not be repeated here one by one.

[0056] Specifically, see Figure 3 and Figure 4The heating device 200 can be an electric heating device or an electromagnetic heating device. In the present utility model, it is preferably an electromagnetic heating device. The electromagnetic heating device includes a heating metal plate 220 and a heating coil 210. The heating metal plate 220 is attached to the side of the temperature control base plate 110 away from the temperature control cylinder 120, and the heating coil 210 is disposed on the side of the heating metal plate 220 away from the temperature control base plate 110. That is, the heating coil 210 can be excited to generate an alternating magnetic field, so that the heating metal plate 220 attached to the temperature control base plate 110 is heated, and the heating metal plate 220 directly transfers the heat to the temperature control base plate 110, thereby realizing electromagnetic heating of the temperature control base 100 by the electromagnetic heating device.

[0057] Specifically, the heating metal plate 220 can be an iron part or an iron alloy part, especially a pure iron part. Pure iron material has a relatively high magnetic permeability (7000-10000) and can quickly generate heat under the high-frequency magnetic field due to the combined action of hysteresis effect and Joule heating effect; at the same time, the thickness of the heating metal plate 220 has a relatively optimal value. If the heating metal plate 220 is too thin and does not match the induced magnetic field, it cannot produce an effective thermal effect. If the heating metal plate 220 is too thick, it will store too much heat and affect the cooling rate of the temperature control base 100. In the utility model, the heating metal plate 220 is best selected as a pure iron sheet with a thickness of 1 mm.

[0058] More specifically, the heating metal plate 220 can be fastened to the temperature control base plate 110 via a threaded connector 221 , and thermal conductive silicone grease can be applied between the two metal joint surfaces to improve heat transfer efficiency.

[0059] In the embodiment of the utility model, the outer diameter of the heating coil 210 is larger than the outer diameter of the heating metal plate 220, so as to obtain better induction heating efficiency. Further, the heating metal plate 220 and the temperature control base plate 110 can be circular plates of the same size, the heating coil 210 is wound into a cylindrical shape, and the outer diameter of the heating coil 210 is slightly larger than the outer diameter of the heating metal plate 220. At the same time, the heating coil 210 is wound with multiple strands of Litz wire.

[0060] In the embodiment of the utility model, a magnetic isolation sheet 230 is provided on the side of the heating coil 210 away from the heating metal plate 220. The addition of the magnetic isolation sheet 230 can be used to reduce the dissipation of the magnetic field and improve the efficiency of induction heating.

[0061] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0062] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0063] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0064] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in the field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A PCR liquid cooling device, characterized in that: The PCR liquid cooling device comprises: The temperature control base (100) is formed with an insertion space (121) for the PCR reaction tube to be inserted and inserted; A liquid cooling head (310) is sleeved on the outer peripheral wall of the insertion space (121) and enclosed with the outer peripheral wall to form a liquid cooling channel (314); a liquid inlet (315) and a liquid outlet (316) in communication with the liquid cooling channel (314) are provided on opposite sides of the liquid cooling head (310); The temperature control cylinder (120) of the temperature control base (100) encloses the insertion space (121) extending in the vertical direction, the liquid cooling head (310) is sleeved on the outside of the temperature control cylinder (120) and encloses the temperature control cylinder (120) to form the liquid cooling channel (314), and the temperature control cylinder (120) is provided with a plurality of detection holes (122) spaced in sequence along the circumferential direction at positions offset from the liquid cooling head (310).

2. The PCR liquid cooling device according to claim 1, characterized in that: The temperature control base (100) comprises a temperature control base plate (110) and a temperature control cylinder (120) arranged on the temperature control base plate (110); the temperature control cylinder (120) is enclosed on the temperature control base plate (110) to form the insertion space (121) extending in the vertical direction; the liquid cooling head (310) is sleeved on the outside of the temperature control cylinder (120) and is enclosed with the temperature control cylinder (120) to form the liquid cooling channel (314).

3. The PCR liquid cooling device according to claim 2, characterized in that: The liquid cooling head (310) comprises a flow channel portion (311), a liquid inlet portion (312) and a liquid outlet portion (313); the flow channel portion (311) is provided with a sleeve hole (317) for the temperature control cylinder (120) to pass through, and the flow channel portion (311) is also provided with an annular groove (318) on the outer side of the sleeve hole (317); the inner wall of the annular groove (318) and the outer peripheral wall of the temperature control cylinder (120) are combined to form the liquid cooling flow channel (314); the liquid inlet portion (312) and the liquid outlet portion (313) are respectively arranged on opposite sides of the flow channel portion (311) and the liquid inlet port (315) and the liquid outlet port (316) are provided one by one.

4. The PCR liquid cooling device according to claim 3, characterized in that: The upper end of the flow channel portion (311) is protruded with a lap boss portion (3111), and the lap boss portion (3111) is arranged around the sleeve hole (317), and the lap boss portion (3111) extends out of the flow channel portion (311) in a direction toward the center line of the sleeve hole (317) to form an extension surface (3112) that overlaps with the upper end surface of the temperature control barrel (120).

5. The PCR liquid cooling device according to claim 2, characterized in that: The temperature control cylinder (120) is provided with a temperature sensor (400) at a position offset from the liquid cooling head (310).

6. The PCR liquid cooling device according to any one of claims 1 to 5, characterized in that: The cross section of the insertion space (121) is arranged to be tapered in the direction from the opening toward the bottom wall; and / or the temperature control base (100) is arranged to be an integrally formed aluminum part.

7. The PCR liquid cooling device according to any one of claims 1 to 5, characterized in that: The PCR liquid cooling device also includes a power component (320), a cooling box (330) and a refrigeration assembly (340), wherein the cooling box (330) is used to store cooling liquid, and the refrigeration assembly (340) is arranged on the cooling box (330), and the power component (320) is used to guide the cooling liquid in the cooling box (330) to the liquid inlet (315), and the liquid outlet (316) flows back to the cooling box (330) through the liquid outlet pipe.

8. The PCR liquid cooling device according to claim 7, characterized in that: The refrigeration component (340) comprises a semiconductor refrigeration sheet (341) provided on the cooling box (330), and a heat dissipation fan (342) provided on the semiconductor refrigeration sheet (341).

9. A PCR detection device, characterized in that: The PCR detection equipment comprises a heating device (200) and a PCR liquid cooling device according to any one of claims 1 to 8, and the heating device (200) is arranged on the temperature control base (100).

Images