Anther tissue sterilization bottle and anther tissue sterilization device

Abstract

The utility model discloses an anther tissue sterilization bottle and anther tissue sterilization device relates to plant artificial breeding field, including bottle body and liquid outlet nozzle, bottle mouth for flower bud tissue is provided on the top of bottle body, the side wall of bottle body is provided with liquid outlet, liquid outlet is connected with liquid outlet nozzle, the liquid outlet nozzle is provided with filter screen inside, the filter screen is connected with the bottle body. According to the anther tissue sterilization bottle provided by the utility model, sterilization liquid can be discharged from the side surface of the sterilization bottle, the operation of discharging the sterilization liquid can be completed without completely reversing the sterilization bottle, mutual extrusion caused by accumulation of flower buds is avoided, and the flower buds are protected from being damaged. The anther tissue sterilization device provided by the utility model comprises a horizontal uniform mixing instrument and the anther tissue sterilization bottle, the horizontal uniform mixing instrument can replace human hands to carry out uniform shaking operation, and the horizontal uniform mixing instrument can continuously output stable and soft shaking force, so that the problem that flower buds are collided and damaged due to overlarge shaking force is avoided.

Description

Technical Field

[0001] The utility model relates to the field of plant artificial propagation, in particular to an anther tissue sterilization bottle and an anther tissue sterilization device. Background Art

[0002] In anther tissue culture, disinfection and sterilization of flower buds separated from petals and sepals is a very important step. Insufficient sterilization can easily lead to anther contamination after inoculation of the culture dish. In the inoculated culture dish, any anther due to inadequate sterilization can also cause indirect contamination of other anthers in the dish, thereby affecting the embryoid induction rate.

[0003] In the prior art, in order to fully sterilize and clean each flower bud, the flower bud is usually placed in a sterilization bottle containing sterilization liquid, and then the sterilization bottle is shaken to fully mix the sterilization liquid and the flower bud to achieve sterilization and cleaning. In this process, the sterilization liquid needs to be replaced multiple times according to actual conditions. The current method of discharging the sterilization liquid is mostly to directly invert the sterilization bottle and discharge it from the top of the sterilization bottle. This discharge method will cause the flower buds to accumulate and produce a strong squeezing effect, resulting in damage to the flower buds. At the same time, since the shaking of the sterilization bottle is operated manually, excessive shaking force will also cause the flower buds to be damaged due to collision with each other. Utility Model Content

[0004] The utility model aims to provide an anther tissue sterilization bottle to solve the problems existing in the above-mentioned prior art, so that the sterilization liquid can be discharged from the side of the sterilization bottle, and the sterilization liquid can be discharged without completely turning the sterilization bottle upside down, thereby avoiding mutual squeezing caused by the accumulation of flower buds and protecting the flower buds from damage.

[0005] The utility model also provides an anther tissue sterilization device, including a horizontal mixer and the above-mentioned anther tissue sterilization bottle. The horizontal mixer can replace the manual shaking operation, and the horizontal mixer can continuously output a stable and gentle shaking force to avoid the problem of excessive shaking force causing flower buds to collide and be damaged.

[0006] To achieve the above purpose, the utility model provides the following solutions:

[0007] The utility model provides an anther tissue sterilization bottle, comprising a bottle body and a liquid outlet nozzle, wherein the top of the bottle body is provided with a bottle mouth for filling flower buds, the side wall of the bottle body is provided with a liquid outlet, the liquid outlet is connected to the liquid outlet nozzle, and a filter is arranged in the liquid outlet nozzle.

[0008] Preferably, the shape of the liquid outlet nozzle is an oblique pointed nozzle, and the outlet direction of the liquid outlet nozzle is obliquely cut upward.

[0009] Preferably, the liquid outlet nozzle is detachable, and a clamping block is provided at one end of the liquid outlet nozzle connected to the bottle body, and a clamp matching the clamping block is provided on the bottle body.

[0010] Preferably, a bottle cap is provided on the bottle mouth, a top surface of the bottle cap is provided with a ventilated area, and the ventilated area is covered with a splash-proof ventilated film.

[0011] Preferably, the side of the bottle cap is provided with anti-slip patterns.

[0012] Preferably, the bottle body is a triangular flask.

[0013] The utility model also provides an anther tissue sterilization device, comprising a horizontal mixer and the anther tissue sterilization bottle. The horizontal mixer comprises a base and a mixing platform arranged on the base, and the anther tissue sterilization bottle is placed on the mixing platform.

[0014] Preferably, the mixing platform is provided with a clamping plate and a clamping rod for clamping the anther tissue sterilization bottle.

[0015] Preferably, the mixing platform is connected to the base via a shaking plate.

[0016] Preferably, a button for controlling the operation of the mixing platform and a display screen for displaying the working status are provided on the side of the base.

[0017] Compared with the prior art, the utility model has achieved the following technical effects:

[0018] The utility model provides an anther tissue sterilization bottle, which enables sterilization liquid to be discharged from the side of the sterilization bottle, and the sterilization liquid can be discharged without completely turning the sterilization bottle upside down, thereby avoiding mutual squeezing caused by accumulation of flower buds and protecting the flower buds from damage.

[0019] The utility model also provides an anther tissue sterilization device, including a horizontal mixer and the above-mentioned anther tissue sterilization bottle. The horizontal mixer can replace the manual shaking operation, and the horizontal mixer can continuously output a stable and gentle shaking force to avoid the problem of excessive shaking force causing flower buds to collide and be damaged. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 This is a schematic diagram of the structure of the anther tissue sterilization bottle in the utility model;

[0022] Figure 2 It is a structural schematic diagram of the liquid outlet nozzle in the utility model;

[0023] Figure 3 It is a structural schematic diagram of the horizontal mixer in the utility model;

[0024] Figure 4 It is a structural schematic diagram of the anther tissue sterilization device in the utility model.

[0025] Among them, 1. bottle body; 11. clip; 2. liquid outlet; 21. filter; 22. card block; 3. bottle cap; 31. splash-proof breathable membrane; 32. anti-slip pattern; 4. horizontal mixer; 41. base; 42. mixing platform; 43. clamping plate; 44. clamping stick; 45. shaking plate; 46. button; 47. display screen. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] The utility model aims to provide an anther tissue sterilization bottle to solve the problems existing in the above-mentioned prior art, so that the sterilization liquid can be discharged from the side of the sterilization bottle, and the sterilization liquid can be discharged without completely turning the sterilization bottle upside down, thereby avoiding mutual squeezing caused by the accumulation of flower buds and protecting the flower buds from damage.

[0028] The utility model also provides an anther tissue sterilization device, including a horizontal mixer and the above-mentioned anther tissue sterilization bottle. The horizontal mixer can replace the manual shaking operation, and the horizontal mixer can continuously output a stable and gentle shaking force to avoid the problem of excessive shaking force causing flower buds to collide and be damaged.

[0029] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0030] like Figure 1 and 2As shown, the utility model is an anther tissue sterilization bottle, comprising a bottle body 1 and a liquid outlet 2, the top of the bottle body 1 is provided with a bottle mouth for filling flower buds, the side wall of the bottle body 1 is provided with a liquid outlet, the liquid outlet is connected to the liquid outlet 2, and a filter screen 21 is arranged in the liquid outlet 2. When in use, the flower buds to be treated are placed in the bottle body 1, and then sterilizing liquid is injected into the bottle body 1, and then the bottle body 1 is shaken to make the flower buds and the sterilizing liquid fully mixed, and the shaking is stopped after a period of time (generally 10 minutes), and the bottle body 1 is tilted to make the sterilizing liquid flow out of the liquid outlet 2, and finally sterile and clean flower buds are obtained, and the sterilizing liquid flows out from the side wall of the bottle body 1, and can be emptied without completely dumping the bottle body 1, so as to avoid the accumulation and squeezing of the flower buds and prevent the flower buds from being damaged, and the filter screen 21 can prevent the flower buds from being discharged from the liquid outlet 2.

[0031] like Figure 2 As shown, the shape of the liquid outlet nozzle 2 is an oblique pointed nozzle, and the outlet direction of the liquid outlet nozzle 2 is obliquely cut upward. The upward liquid outlet nozzle 2 has the function of preventing the sterilizing liquid from splashing, that is, when droplets of sterilizing liquid fly out from the liquid outlet during the shaking process, they can be received by the liquid outlet nozzle 2 and flow back into the bottle body 1, and will not pollute the surrounding environment.

[0032] like Figure 1 and 2 As shown, the liquid outlet nozzle 2 is detachable, and a block 22 is provided at one end of the liquid outlet nozzle 2 connected to the bottle body 1, and a clip 11 matching the block 22 is provided on the bottle body 1. During installation, the gap of the block 22 is first aligned with the clip 11, and then the liquid outlet nozzle 2 is rotated so that the block 22 enters the clip 11 to complete the installation. In a preferred embodiment, the number of the clip 11 and the block 22 are both 4.

[0033] like Figure 1 As shown, a bottle cap 3 is arranged on the bottle mouth, a ventilation area is opened on the top surface of the bottle cap 3, and the ventilation area is covered with a splash-proof breathable membrane 31, the splash-proof breathable membrane 31 can prevent the sterilizing liquid droplets from splashing into the external environment during the shaking process, and the bottle cap 3 can play an air intake role when the sterilizing liquid is discharged, thereby increasing the discharge speed of the sterilizing liquid. In a preferred embodiment, the pore size of the splash-proof breathable membrane 31 is 0.2μm.

[0034] like Figure 1 As shown, the side of the bottle cap 3 is provided with anti-slip grooves 32 to improve the operating reliability of the operator. In a preferred embodiment, the bottle cap 3 is a threaded cap, which is easy to open and close and not easy to loosen.

[0035] like Figure 1 As shown, the bottle body 1 is a triangular bottle, and the shape of the bottle body 1 can also be other different shapes that can meet the needs of use.

[0036] In a preferred embodiment, the bottle body 1, the liquid outlet nozzle 2 and the bottle cap 3 are made of polycarbonate (PC) or polyethylene terephthalate-1,4-cyclohexane dimethanol (PETE), avoiding the use of bisphenol A. The sterilizing liquid used in the utility model is a mercuric chloride solution with a volume fraction of 1 / 1000.

[0037] like Figure 3 and 4 As shown, the utility model also provides an anther tissue sterilization device, including a horizontal mixer 4 and the above-mentioned anther tissue sterilization bottle, the horizontal mixer 4 includes a base 41 and a mixing platform 42 arranged on the base 41, the anther tissue sterilization bottle is placed on the mixing platform 42, and a motion motor is installed in the base 41 to drive the mixing platform 42 to shake, and the mixing platform 42 drives the bottle body 1 placed thereon to shake together to achieve mixing of the flower buds and the sterilization solution.

[0038] like Figure 3 As shown, the mixing platform 42 is provided with a clamping plate 43 and a clamping rod 44 for clamping the anther tissue sterilization bottle, and the clamping plate 43 and the clamping rod 44 enclose an area for placing the bottle body 1. The clamping plate 43 and the clamping rod 44 can limit the area of ​​the bottle body 1 to prevent the bottle body 1 from detaching from the mixing platform 42 during shaking.

[0039] like Figure 3 and 4 As shown, the mixing platform 42 is connected to the base 41 through a shaking plate 45, one end of the shaking plate 45 is connected to the bottom surface of the mixing platform 42, and the other end of the shaking plate 45 extends into the base 41 and is connected to the motion motor. The shaking plate 45 can drive the mixing platform 42 to shake.

[0040] like Figure 3 and 4 As shown, a button 46 for controlling the operation of the mixing platform 42 and a display screen 47 for displaying the working status are provided on the side of the base. The button 46 is used to control the start and stop of the motion motor and adjust the working parameters of the motion motor. The display screen 47 can display data such as working time and working parameters.

[0041] The present invention uses specific examples to illustrate the principle and implementation of the present invention. The above examples are only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. An anther tissue sterilization bottle, characterized in that: The invention comprises a bottle body (1) and a liquid outlet nozzle (2); the top of the bottle body (1) is provided with a bottle mouth for flower bud tissue; the side wall of the bottle body (1) is provided with a liquid outlet, the liquid outlet is connected to the liquid outlet nozzle (2); and a filter screen (21) is arranged inside the liquid outlet nozzle (2).

2. The anther tissue sterilization bottle according to claim 1, characterized in that: The shape of the liquid outlet nozzle (2) is an oblique pointed nozzle, and the outlet direction of the liquid outlet nozzle (2) is obliquely upward.

3. The anther tissue sterilization bottle according to claim 1, characterized in that: The liquid outlet nozzle (2) is detachable, and a clamping block (22) is provided at one end of the liquid outlet nozzle (2) connected to the bottle body (1), and a clamp (11) matching the clamping block (22) is provided on the bottle body (1).

4. The anther tissue sterilization bottle according to claim 1, characterized in that: A bottle cap (3) is arranged on the bottle mouth, a top surface of the bottle cap (3) is provided with a ventilating area, and the ventilating area is covered with a splash-proof ventilating film (31).

5. The anther tissue sterilization bottle according to claim 4, characterized in that: The side surface of the bottle cap (3) is provided with anti-slip grooves (32).

6. The anther tissue sterilization bottle according to claim 1, characterized in that: The bottle body (1) is a triangular bottle.

7. An anther tissue sterilization device, characterized in that: It comprises a horizontal mixer (4) and the anther tissue sterilization bottle according to any one of claims 1 to 6, wherein the horizontal mixer (4) comprises a base (41) and a mixing platform (42) arranged on the base (41), and the anther tissue sterilization bottle is placed on the mixing platform (42).

8. The anther tissue sterilization device according to claim 7, characterized in that: The mixing platform (42) is provided with a clamping plate (43) and a clamping rod (44) for clamping the anther tissue sterilization bottle.

9. The anther tissue sterilization device according to claim 7, characterized in that: The mixing platform (42) is connected to the base (41) via a shaking plate (45).

10. The anther tissue sterilization device according to claim 7, characterized in that: A button (46) for controlling the operation of the mixing platform (42) and a display screen (47) for displaying the working status are arranged on the side of the base.

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