Polypeptide crude product washing filter

Abstract

The utility model relates to the technical field of polypeptide preparation, and particularly discloses a polypeptide crude product washing filter which comprises a washing box, the washing assembly is arranged in the washing chamber and is used for synthesizing solid-phase peptide and adding a proper solvent for washing so as to remove unreacted amino acid and other impurities; the filter box is arranged at the bottom end of the washing box; the motor drives the fixing rod to rotate slowly, the heating plate continuously stirs a solvent and a polypeptide crude product in the heating plate, certain chemical reactions of polypeptide are promoted through heating, the polypeptide crude product and the added solvent are fully mixed, the washing efficiency is improved, meanwhile, the scraping rod rotates synchronously along with the heating plate, polypeptide mucus and impurities attached to the interior of the reaction barrel are scraped off, and the cleaning effect is improved. The conditions that impurities and polypeptide mucus block filter holes, washed polypeptide cannot pass through the filter holes, flow is reduced or completely stopped, and the polypeptide washing efficiency is reduced are prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of polypeptide preparation, in particular to a polypeptide crude product washing filter. Background Art

[0002] Crude peptide is a stage in the peptide preparation process, which refers to the peptide mixture obtained after preliminary extraction and separation by a specific method. The product of this stage usually contains the target peptide and other impurities, which need to be further purified and analyzed to achieve the required purity and quality. Filtration of crude peptide is an important step in the preparation process, mainly used to remove impurities and unreacted substances in order to obtain a purer peptide.

[0003] Since the polypeptide solution is viscous, during the filtration process, the polypeptide solution and other impurities are easily attached to the inner wall of the filter, causing the filter to be blocked, the filter flow rate to be reduced or completely stopped, and the filtration effect to be greatly reduced. For this reason, we propose a polypeptide crude product washing filter. Utility Model Content

[0004] The utility model aims to provide a crude polypeptide washing filter to solve the problem in the above-mentioned background technology that, due to the viscosity of the polypeptide solution, during the filtration process, the polypeptide solution and other impurities are easily attached to the inner wall of the filter, resulting in filter blockage, reduced or completely stopped filtration flow, and significantly reduced filtration effect.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a crude polypeptide washing filter, comprising a washing box;

[0006] Also includes:

[0007] A washing component is disposed inside the washing chamber and is used to add a suitable solvent to the solid phase peptide synthesis for washing to remove unreacted amino acids and other impurities;

[0008] The filter box is arranged at the bottom of the washing box, and the filter box separates the polypeptide from the impurities through adsorption of different chromatography materials.

[0009] Among them, the washing component includes a reaction barrel, the outer wall of the reaction barrel is provided with multiple filter holes at equal intervals, a motor is installed at the top of the reaction barrel, a fixing rod is arranged at the bottom of the motor, two heating plates are fixedly connected to the outer wall of the fixing rod, and the top of the heating plates are fixedly connected with a scraper rod, and the outer wall of the scraper rod is in contact with the inner wall of the reaction barrel.

[0010] The bottom end of the reaction barrel is fixedly connected with a sewage pipe, and a ball valve is installed on the outer wall of the sewage pipe.

[0011] Among them, a plurality of discharge pipes are distributed in a circular array at the bottom end of the washing box, and the discharge pipes are connected to the washing box and the filter box respectively.

[0012] Among them, a plurality of ball bearings are arranged in a circular array on the inner wall of the bottom end of the washing box, a movable ring is arranged at the bottom end of the washing box, a movable groove is opened on the outer wall of the movable ring, the movable groove is movably connected with the ball bearings, and a plurality of discharge ports are opened on the outer wall of the movable ring at equal intervals, and the positions of the discharge ports correspond to the positions of the discharge pipes.

[0013] Among them, a tooth groove is opened on the inner wall of the movable ring, the running track of the tooth groove matches the running track of the discharge port, and the inner wall of the tooth groove is meshed and connected with an adjusting gear.

[0014] Among them, a plurality of fixing frames are fixedly connected to the outer wall of the washing box, and the fixing frames are distributed in a circular array with respect to the outer wall of the washing box. An electric telescopic rod is installed at the bottom end of each fixing frame, and a processing box is fixedly connected to the bottom end of the electric telescopic rod. The internal size of the processing box is consistent with the internal size of the filter box.

[0015] Among them, a movable cover is threadedly connected to the top of the processing box, and a pressing ring is arranged above the movable cover, and the pressing ring is fixedly connected to the filter box.

[0016] The utility model has at least the following beneficial effects:

[0017] The motor drives the fixed rod to rotate slowly, so that the heating plate continuously stirs the solvent and the crude polypeptide therein, heating to promote certain chemical reactions of the polypeptide, while fully mixing the crude polypeptide with the added solvent to improve the washing efficiency. At the same time, the scraper rod rotates synchronously with the heating plate to scrape off the polypeptide mucus and impurities attached to the inside of the reaction barrel to prevent impurities and polypeptide mucus from clogging the filter holes. The washed polypeptide cannot pass through the filter holes, resulting in a reduction in flow or a complete stop, which reduces the polypeptide washing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the front view structure of the utility model;

[0019] Figure 2 This is a side view of the structure of the utility model;

[0020] Figure 3 This is a schematic diagram of the cutaway structure of the washing box of the utility model;

[0021] Figure 4 This is a schematic diagram of the explosion structure of the washing box of the utility model;

[0022] Figure 5 This is a schematic diagram of the structure of the movable ring of the utility model;

[0023] Figure 6 This is a schematic diagram of the explosion structure of the filter box of the utility model;

[0024] Figure 7 For this utility model Figure 5 Enlarged structural diagram at A in the middle.

[0025] In the figure: 001, washing box; 002, washing component; 003, filter box; 201, reaction barrel; 202, filter hole; 203, motor; 204, fixed rod; 205, heating plate; 206, scraper rod; 210, drain pipe; 220, ball valve; 230, ball; 240, movable ring; 250, movable groove; 260, discharge port; 270, tooth groove; 280, adjusting gear; 310, discharge pipe; 410, fixed frame; 420, electric telescopic rod; 430, processing box; 440, movable cover; 450, pressing ring. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] See also Figures 1 to 7 , the utility model provides a technical solution:

[0028] Embodiment 1

[0029] A polypeptide crude product washing filter, comprising a washing box 001;

[0030] Also includes:

[0031] The washing component 002 is disposed inside the washing chamber and is used to synthesize solid-phase peptides and add appropriate solvents for washing to remove unreacted amino acids and other impurities;

[0032] Filter box 003: Filter box 003 is arranged at the bottom of washing box 001. Filter box 003 separates polypeptides from impurities through adsorption of different chromatography materials.

[0033] Among them, a hopper is set at the top of the washing box 001. The staff first puts the crude polypeptide and the added solvent into the reaction barrel 201 through the feed hopper. The washing component 002 can slowly stir the crude polypeptide to ensure that the solvent and the crude polypeptide are fully mixed. Large pieces of impurities are intercepted in the reaction barrel 201. The impurities can be discharged through the drain pipe 210. The washed crude polypeptide will enter the filter box 003 and then be finally filtered by the processing box 430 to obtain a high-purity polypeptide.

[0034] The washing component 002 includes a reaction barrel 201, and a plurality of filter holes 202 are provided on the outer wall of the reaction barrel 201 at equal intervals. A motor 203 is installed on the top of the reaction barrel 201, and a fixing rod 204 is provided on the bottom end of the motor 203. Two heating plates 205 are fixedly connected to the outer wall of the fixing rod 204, and scraping rods 206 are fixedly connected to the tops of the heating plates 205. The outer wall of the scraping rod 206 fits the inner wall of the reaction barrel 201.

[0035] A sewage pipe 210 is fixedly connected to the bottom end of the reaction barrel 201 , and a ball valve 220 is installed on the outer wall of the sewage pipe 210 .

[0036] The motor 203 drives the fixed rod 204 to rotate slowly, so that the heating plate 205 continuously stirs the solvent and the crude polypeptide therein to fully mix them and dissolve the crude polypeptide. At the same time, the heating plate 205 releases appropriate heat to promote certain chemical reactions of the polypeptide. It can also be used for sterilization or removal of heat-unstable impurities to improve washing efficiency. When the heating plate 205 is stirring, the two scraping rods 206 can scrape off the polypeptide mucus and impurities attached to the inside of the reaction barrel 201 to prevent the impurities from clogging the filter hole 202 and reducing the washing efficiency of the polypeptide.

[0037] After the device has been running for a period of time, the staff can open the ball valve 220 and rinse the reaction barrel 201 with clean water, so that impurities are discharged to the outside through the sewage pipe 210 under water pressure. The arrangement is convenient for cleaning the reaction barrel 201 to avoid impurities remaining inside the reaction barrel 201, which may have an adverse effect on the filtration of polypeptides.

[0038] A plurality of discharge pipes 310 are distributed in a circular array at the bottom of the washing box 001 , and the discharge pipes 310 are connected to the washing box 001 and the filter box 003 respectively.

[0039] A plurality of balls 230 are arranged in a circular array on the inner wall of the bottom end of the washing box 001, and a movable ring 240 is arranged at the bottom end of the washing box 001. A movable groove 250 is arranged on the outer wall of the movable ring 240, and the movable groove 250 is movably connected to the balls 230. A plurality of discharge ports 260 are arranged on the outer wall of the movable ring 240 at equal intervals, and the positions of the discharge ports 260 correspond to the position of the discharge pipe 310.

[0040] The inner wall of the movable ring 240 is provided with a tooth groove 270 , the running track of the tooth groove 270 matches the running track of the discharge port 260 , and the inner wall of the tooth groove 270 is meshedly connected with an adjusting gear 280 .

[0041] Among them, the staff can rotate the adjustment gear 280, and through the mutual cooperation between the adjustment gear 280 and the tooth groove 270, the movable ring 240 can rotate inside the washing box 001. When the adjustment gear 280 rotates to the bottom of the tooth groove 270, the discharge port 260 corresponds to the position of the discharge pipe 310, and the washed polypeptide enters the filter box 003. The arrangement is convenient for multiple discharge pipes 310 to discharge at the same time. The polypeptide can be evenly distributed inside the filter box 003, so that the filtration pressure at each position of the filter box 003 is the same, thereby ensuring the stability of the filtration efficiency of the filter box 003 and avoiding the waste of internal resources of the filter box 003.

[0042] The ball 230 facilitates the rotation of the movable ring 240 and can limit the upper and lower positions of the movable ring 240 so that the movable ring 240 can completely close the discharge pipe 310 and improve the sealing performance of the washing box 001.

[0043] Embodiment 2

[0044] In the second embodiment, other structures remain unchanged. The difference from the first embodiment is that a plurality of fixing frames 410 are fixedly connected to the outer wall of the washing box 001. The fixing frames 410 are distributed in a circular array with respect to the outer wall of the washing box 001. An electric telescopic rod 420 is installed at the bottom end of each fixing frame 410. A processing box 430 is fixedly connected to the bottom end of the electric telescopic rod 420. The internal dimensions of the processing box 430 are consistent with the internal dimensions of the filter box 003.

[0045] The top of the processing box 430 is threadedly connected with a movable cover 440 , and a pressing ring 450 is provided above the movable cover 440 . The pressing ring 450 is fixedly connected to the filter box 003 .

[0046] Among them, the staff can put the filter cloth on the top of the processing box 430, and then connect the movable cover 440 with the processing box 430 to fix the filter cloth. After starting the electric telescopic rod 420, the processing box 430 is driven to dock with the filter box 003. At the same time, the pressing ring 450 can separate the filter cloth and the filter box 003 for a distance, so that the polypeptide can flow quickly and fill every corner of the filter cloth, thereby improving the filtering effect of the filter cloth. After the polypeptide enters the interior of the filter box 003, it is finally filtered by the filter cloth. Multiple filtrations make the polypeptide purer and can effectively prevent impurities from clogging the device.

[0047] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0048] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A polypeptide crude product washing filter, comprising: Washing box (001); It is characterized by: also including: A washing component (002), the washing component (002) is arranged inside the washing chamber, and the washing component (002) is used to synthesize solid-phase peptides and add appropriate solvents for washing to remove unreacted amino acids and other impurities; A filter box (003), wherein the filter box (003) is disposed at the bottom of the washing box (001), and the filter box (003) separates the polypeptide from impurities by adsorption of different chromatography materials; The washing component (002) comprises a reaction barrel (201), the outer wall of the reaction barrel (201) is provided with a plurality of filter holes (202) at equal intervals, a motor (203) is installed at the top end of the reaction barrel (201), a fixing rod (204) is arranged at the bottom end of the motor (203), the outer wall of the fixing rod (204) is fixedly connected to two heating plates (205), the top ends of the heating plates (205) are fixedly connected to scraping rods (206), and the outer wall of the scraping rod (206) is in contact with the inner wall of the reaction barrel (201).

2. The crude polypeptide washing filter according to claim 1, characterized in that: The bottom end of the reaction barrel (201) is fixedly connected to a sewage pipe (210), and a ball valve (220) is installed on the outer wall of the sewage pipe (210).

3. The crude polypeptide washing filter according to claim 1, characterized in that: A plurality of discharge pipes (310) are distributed in a circular array at the bottom end of the washing box (001), and the discharge pipes (310) are respectively connected to the washing box (001) and the filter box (003).

4. The crude polypeptide washing filter according to claim 3, characterized in that: A plurality of balls (230) are arranged in a circular array on the inner wall of the bottom end of the washing box (001); a movable ring (240) is arranged at the bottom end of the washing box (001); a movable groove (250) is arranged on the outer wall of the movable ring (240); the movable groove (250) is movably connected to the balls (230); a plurality of discharge ports (260) are arranged on the outer wall of the movable ring (240) at equal intervals; the positions of the discharge ports (260) correspond to the positions of the discharge pipe (310).

5. The crude polypeptide washing filter according to claim 4, characterized in that: The inner wall of the movable ring (240) is provided with a tooth groove (270), the running track of the tooth groove (270) matches the running track of the discharge port (260), and the inner wall of the tooth groove (270) is meshedly connected with an adjusting gear (280).

6. The crude polypeptide washing filter according to claim 1, characterized in that: A plurality of fixing frames (410) are fixedly connected to the outer wall of the washing box (001), and the fixing frames (410) are distributed in a circular array with respect to the outer wall of the washing box (001). An electric telescopic rod (420) is installed at the bottom end of each fixing frame (410), and a processing box (430) is fixedly connected to the bottom end of the electric telescopic rod (420), and the internal dimensions of the processing box (430) are consistent with the internal dimensions of the filter box (003).

7. The crude polypeptide washing filter according to claim 6, characterized in that: A movable cover (440) is threadedly connected to the top of the processing box (430), and a pressing ring (450) is arranged above the movable cover (440). The pressing ring (450) is fixedly connected to the filter box (003).

Images