Microimaging system and time difference incubator
By setting up the optimized layout of the imaging module and the lighting module in the microscope imaging system of the time difference in incubator, and using the optical path adjustment module to fold the optical path, the Hoffman imaging system is formed, which solves the problems of large space occupied by the microscope imaging system, and the microscope imaging system in traditional technology, achieving a more efficient imaging effect.
Patent Information
- Application Number
- CN202421720281.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-19
- Publication Date
- 2025-04-22
- Estimated Expiration
- 2034-07-19
AI Technical Summary
The microscopic imaging system of traditional jet lag incubators takes up a large space due to the direct structure, and the imaging accuracy and quality are low.
A microscopic imaging system is adopted, wherein the imaging module is arranged below the optical path adjustment module, the illumination module is arranged on one side of the imaging module in the horizontal direction, the optical path is folded through the optical path adjustment module, and a Hoffman imaging system is formed using a slit plate, an objective lens and an objective lens modulation plate.
It reduces the illumination module's occupation of space above the petri dish, improves imaging accuracy and quality, and solves the problems of large space and low accuracy in traditional technology.
Smart Images
Figure CN222784621U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of medical equipment, in particular to a microscopic imaging system and a time-difference incubator. Background Art
[0002] In the field of biological sample cultivation, the time-difference incubator can provide a relatively closed culture and development environment for embryos that is close to the mother's uterus, and can achieve real-time monitoring and control of the temperature, CO2 and O2 concentrations of the culture environment. At the same time, a microscopic imaging system is required. When the incubator is closed, the embryo images are taken at regular intervals to fully record the development status of the embryo at each stage, and the embryo morphological parameters are identified through software image algorithms, so as to complete the evaluation, analysis and screening of the embryo.
[0003] The microscopic imaging module in the time-lapse incubator is the most critical part. Only by taking three-dimensional and layered images of biological tissues with the camera can the morphology and development of biological tissues be accurately judged, and the accuracy of embryo evaluation and screening be improved. In related technologies, traditional time-lapse incubators will respectively set up lighting systems and imaging systems on both sides of the culture dish, and corresponding lenses will be set in the lighting system and imaging system to realize the imaging of highly transparent embryo samples using Hoffman imaging technology.
[0004] The time-difference incubator in the related art is used to image highly transparent embryo samples. The traditional microscopic imaging system used is a direct-type structure. The lighting system and the imaging system are located on the upper and lower sides of the culture dish, respectively, which will increase the longitudinal volume and occupied space of the time-difference incubator. When embryos at different positions in the time-difference incubator need to be imaged, the internal motor system needs to drive the lighting module and the imaging module to move relative to the culture dish, respectively. The overall movement accuracy is low, which in turn affects the imaging accuracy and quality. Utility Model Content
[0005] The embodiment of the utility model provides a microscopic imaging system and a time-difference incubator, which can solve the technical problems of large occupied space and low imaging accuracy and quality caused by structural defects of the lighting and imaging systems in the time-difference incubator in the related art. The technical solution is as follows:
[0006] In a first aspect, an embodiment of the utility model provides a microscopic imaging system, comprising:
[0007] Imaging module, light path adjustment module, lighting module and culture dish,
[0008] The imaging module is arranged below the optical path adjustment module, and comprises an objective lens, an objective lens modulation plate, a lens barrel lens and an image collector arranged at intervals in a direction away from the optical path adjustment module, and the culture dish is located between the imaging module and the optical path adjustment module;
[0009] The illumination module is arranged at one side of the imaging module in the horizontal direction, and comprises an illumination light source, a slit plate and a focusing system arranged at intervals in a direction close to the optical path adjustment module, wherein the slit plate is provided with a shaping slit conjugate with the objective lens modulation plate;
[0010] A reflective device group is arranged in the optical path adjustment module, which is used to guide the illumination spot emitted by the illumination light source so as to irradiate the culture dish.
[0011] Optionally, the illumination module is located below the optical path adjustment module and arranged in parallel with the imaging module, the reflective device group includes a first reflector and a second reflector arranged at an angular interval, the first reflector is located above the illumination module, and the second reflector is located above the culture dish.
[0012] Optionally, the microscopic imaging system also includes an adjustment mechanism and a first slide rail, the illumination module is connected to the imaging module via the adjustment mechanism to achieve sliding adjustable spacing, the illumination module is slidably mounted on the first slide rail, and the sliding direction of the illumination module is parallel to the sliding direction of the imaging module.
[0013] Optionally, the microscopic imaging system further includes a second slide rail, the first reflector and the second reflector are both beam splitters, a light-shielding slit plate is arranged between the first reflector and the second reflector, and the illumination module and the imaging module can be slidably mounted on the second slide rail.
[0014] Optionally, the lighting module is located beside the light path adjustment module in the horizontal direction, the reflective device group includes a beam splitter and a third reflector arranged in parallel and spaced apart along the light path of the illumination light source, and a compensation glass is provided below the third reflector.
[0015] Optionally, the microscopic imaging system also includes a third slide rail, and a plurality of groups of embryo placement holes are provided on the culture dish, each group of the embryo placement holes includes a plurality of embryo placement holes arranged at intervals along a first direction, and the plurality of groups of embryo placement holes are arranged at intervals along a second direction perpendicular to the first direction, and the third slide rail is arranged along the second direction, and the lighting module and the imaging module can be slidably mounted on the third slide rail.
[0016] Optionally, the clear aperture of the slit plate is adjustable.
[0017] In a second aspect, an embodiment of the utility model provides a time-difference incubator, comprising the microscopic imaging system described in the first aspect, and also comprising a culture chamber and an incubator shell, wherein the culture chamber is installed above the incubator shell, the optical path adjustment module is arranged in the culture chamber, the culture dish is arranged at the bottom of the culture chamber, and the imaging module and the lighting module are arranged in the incubator shell.
[0018] Optionally, a plurality of the culture chambers are arranged on the shell of the incubator, and the imaging module and the lighting module are configured to perform biaxial movement in the horizontal direction.
[0019] The beneficial effects brought by the technical solution provided by the embodiment of the utility model include at least:
[0020] The microscopic imaging system provided by the embodiment of the utility model is adopted, and a slit plate and an objective lens modulation plate behind the objective lens are respectively arranged in the illumination module and the imaging module, and a Hoffman imaging system is formed by using the slit plate, the objective lens and the objective lens modulation plate to image the sample in the culture dish. Compared with the direct-type structure in the microscopic imaging system in the conventional time-difference incubator, the relative position between the illumination module and the imaging module and the culture dish is optimized, the illumination module is arranged on one side of the imaging module in the horizontal direction, and the optical path is folded by the optical path adjustment module to ensure normal imaging. The occupation of the space above the culture dish by the illumination module can be greatly reduced. When it is necessary to image embryos in different positions, the illumination module and the imaging module can also be moved simultaneously on the same side of the culture dish with the help of the internal transmission system of the time-difference incubator. It is only necessary to ensure that the optical path transmission between the two and the optical path adjustment module is unobstructed, thereby improving the overall motion accuracy, and effectively solving the technical problems of large occupied space, low imaging accuracy and quality caused by the structural defects of the illumination and imaging systems in the time-difference incubator in the related art. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.
[0022] Figure 1 It is a structural schematic diagram of a microscopic imaging system provided by an embodiment of the utility model;
[0023] Figure 2 is a structural schematic diagram of a second microscopic imaging system provided by an embodiment of the utility model;
[0024] Figure 3It is a structural schematic diagram of a mobile control module provided in an embodiment of the present utility model for a microscopic imaging system;
[0025] Figure 4 It is a schematic diagram of the structure of a culture dish provided in an embodiment of the utility model;
[0026] Figure 5 It is a structural schematic diagram of a third microscopic imaging system provided by an embodiment of the utility model;
[0027] Figure 6 It is a structural schematic diagram of a fourth microscopic imaging system provided by an embodiment of the utility model;
[0028] Figure 7 is a structural schematic diagram of a fifth microscopic imaging system provided by an embodiment of the utility model;
[0029] Figure 8 It is a structural schematic diagram of a time difference incubator provided by an embodiment of the utility model.
[0030] In the figure:
[0031] 1-imaging module; 2-light path adjustment module; 3-illumination module; 4-culture dish; 5-adjustment mechanism; 6-first slide rail; 7-second slide rail; 8-third slide rail; 11-objective lens; 12-objective lens modulation plate; 13-tube lens; 14-image collector; 21-reflection device group; 31-illumination light source; 32-slit plate; 33-focusing system; 41-embryo placement hole; 211-first reflector; 212-second reflector; 213-light-shielding slit plate; 214-beam splitter; 215-third reflector; 216-compensation glass; a-culture chamber; b-culture box shell. DETAILED DESCRIPTION
[0032] In order to make the purpose, technical solution and advantages of the present invention more clear, the implementation mode of the present invention will be further described in detail below with reference to the accompanying drawings.
[0033] The microscopic imaging module in the time-lapse incubator is the most critical part. Only by taking three-dimensional and layered images of biological tissues with the camera can the morphology and development of biological tissues be accurately judged, and the accuracy of embryo evaluation and screening be improved. In related technologies, traditional time-lapse incubators will respectively set up lighting systems and imaging systems on both sides of the culture dish, and corresponding lenses will be set in the lighting system and imaging system to realize the imaging of highly transparent embryo samples using Hoffman imaging technology.
[0034] The time-difference incubator in the related art is used to image highly transparent embryo samples. The traditional microscopic imaging system used is a direct-type structure. The lighting system and the imaging system are located on the upper and lower sides of the culture dish, respectively, which will increase the longitudinal volume and occupied space of the time-difference incubator. When embryos at different positions in the time-difference incubator need to be imaged, the internal motor system needs to drive the lighting module and the imaging module to move relative to the culture dish, respectively. The overall movement accuracy is low, which in turn affects the imaging accuracy and quality.
[0035] Figure 1 It is a structural schematic diagram of a microscopic imaging system provided by an embodiment of the utility model; Figure 2 is a structural schematic diagram of a second microscopic imaging system provided by an embodiment of the utility model; Figure 3 It is a structural schematic diagram of a mobile control module provided in an embodiment of the present utility model for a microscopic imaging system; Figure 4 It is a schematic diagram of the structure of a culture dish provided in an embodiment of the utility model; Figure 5 It is a structural schematic diagram of a third microscopic imaging system provided by an embodiment of the utility model; Figure 6 It is a structural schematic diagram of a fourth microscopic imaging system provided by an embodiment of the utility model; Figure 7 is a structural schematic diagram of a fifth microscopic imaging system provided by an embodiment of the utility model; Figure 8 This is a schematic diagram of the structure of a time difference incubator provided by an embodiment of the utility model. Figures 1 to 8 As shown, in order to address the above-mentioned defects, an embodiment of the utility model provides a microscopic imaging system, including: an imaging module 1, an optical path adjustment module 2, an illumination module 3 and a culture dish 4.
[0036] The imaging module 1 is arranged below the optical path adjustment module 2, and includes an objective lens 11, an objective lens modulation plate 12, a tube lens 13 and an image collector 14 arranged at intervals in a direction away from the optical path adjustment module 2, and the culture dish 4 is located between the imaging module 1 and the optical path adjustment module 2.
[0037] The illumination module 3 is arranged on one side of the imaging module 1 in the horizontal direction, and includes an illumination light source 31, a slit plate 32 and a focusing system 33 arranged at intervals along a direction close to the optical path adjustment module 2. The slit plate 32 is provided with a shaping slit conjugate with the objective lens modulation plate 12.
[0038] A reflective device group 21 is disposed in the optical path adjustment module 2 for guiding the illumination spot emitted by the illumination light source 31 to irradiate the culture dish 4 .
[0039] In the embodiment of the utility model, the microscopic imaging system is arranged in a time-difference incubator, which includes a culture chamber a and an incubator shell b, wherein the culture chamber a is arranged on the top of the incubator shell b, the optical path adjustment module 2 is integrated in the culture chamber a, and the imaging module 1 is arranged in the incubator shell b. When it is necessary to perform microscopic imaging on the embryo sample, the staff first installs the culture dish 4 containing the embryo sample into the light-transmitting position between the bottom of the culture chamber a and the incubator shell b, and when the culture dish 4 is installed in place, it is located between the optical path adjustment module 2 and the imaging module 1.
[0040] refer to Figure 1 In a possible implementation of the present invention, the illumination module 3 is located below the optical path adjustment module 2 and arranged in parallel with the imaging module 1. The reflective device group 21 includes a first reflector 211 and a second reflector 212 arranged at an angle interval. The first reflector 211 is located above the illumination module 3, and the second reflector 212 is located above the culture dish 4. When imaging cells with high transparency, the illumination module 3 is started, and the illumination light source 31 is used to emit light. When the light passes through the shaping slit of the slit plate 32, it is blocked to a certain extent, and only part of the light of the illumination spot is allowed to pass through the shaping slit. After passing through the focusing system 33, the light is injected into the optical path adjustment module 2 from bottom to top. After being reflected twice by the first reflector 211 and the second reflector 212 spaced in the horizontal direction and arranged at an angle of 90° to each other, the light is irradiated on the embryo sample in the culture dish 4, generating diffraction and refraction. The light further enters the imaging module 1 located below the culture dish 4, and after passing through the rear focal plane of the objective lens 11, it is adjusted to the corresponding optimal imaging position using the structure of the shaping slit and the objective lens modulation plate 12 to form a Hoffman imaging system, forming three areas of dark, gray and bright on the objective lens modulation plate 12, and ultimately causing the refracted and diffracted light at different angles to hit different areas of the objective lens modulation plate 12, thereby causing the transparent sample to produce light and dark changes, and the imaging has a relief effect, and finally passes through the tube lens 13 and uses the image collector 14 to image the sample, and by using the coordinated structure composed of the objective lens 11 and the objective lens modulation plate 12, the imaging capability of the transparent sample is effectively improved, and the three-dimensional sense and layering of the microscopic imaging are improved.
[0041] The microscopic imaging system provided by the embodiment of the utility model is used to image the sample in the culture dish 4 by respectively setting a slit plate 32 and an objective lens modulation plate 12 behind the objective lens 11 in the illumination module 3 and the imaging module 1, and using the slit plate 32, the objective lens 11 and the objective lens modulation plate 12 to form a Hoffman imaging system. Compared with the direct-type structure in the microscopic imaging system in the conventional time-difference incubator, the relative positions between the illumination module 3 and the imaging module 1 and the culture dish 4 are optimized, the illumination module 3 is set on one side of the imaging module 1 in the horizontal direction, and the optical path is folded by the optical path adjustment module 2 to ensure normal imaging. The space occupied by the lighting module 3 above the culture dish 4 can be greatly reduced. When it is necessary to image embryos in different positions, the lighting module 3 and the imaging module 1 can also be moved simultaneously on the same side of the culture dish 4 with the help of the internal transmission system of the time difference incubator. It is only necessary to ensure that the light path transmission between the two and the light path adjustment module 2 is unobstructed, thereby improving the overall movement accuracy and effectively solving the technical problems of large space occupation, low imaging accuracy and quality caused by structural defects of the lighting and imaging systems in the time difference incubator in the related technology.
[0042] Optionally, the microscopic imaging system further includes an adjustment mechanism 5 and a first slide rail 6, the illumination module 3 is connected to the imaging module 1 through the adjustment mechanism 5 to achieve adjustable sliding distance, the illumination module 3 is slidably mounted on the first slide rail 6, and the sliding direction of the illumination module 3 is parallel to the sliding direction of the imaging module 1. For example, in the second possible implementation of the present invention, with reference to Figure 2 , the imaging module 1 is installed on the first slide rail 6 through a sliding motor, and the lighting module 3 and the imaging module 1 are both arranged below the optical path adjustment module 2, wherein the lighting module 3 is connected to one side of the imaging module 1 in the horizontal direction through an adjustment mechanism 5 composed of a transmission structure such as a slide rail bracket and an electric push rod. When the imaging module 1 slides along the first slide rail 6, the lighting module 3 can also adjust the individual spacing in the horizontal direction relative to the imaging module 1. When there are multiple embryo samples in the culture dish 4, for example, when there are two embryo samples in the extension direction of the first slide rail 6, the components in the lighting module 3 and the imaging module 1 are located Figure 2 When the imaging module 1 is at the first position indicated by the dotted line in the figure, the sample on the left side of the culture dish 4 can be imaged. By adjusting the spacing between the imaging module 1 on the first slide rail 6 and the illumination module 3 and the imaging module 1, the components in the illumination module 3 and the imaging module 1 are positioned at the same position. Figure 2When the second position is a solid line in the middle, the sample on the right side of the culture dish 4 can be imaged. By setting the above structure, by adjusting the relative positions of the illumination module 3 and the imaging module 1 and the optical path adjustment module 2 relatively fixed in the time difference incubator, it is possible to image the positions of multiple embryo samples in the culture dish 4 separately, and at the same time, the entire optical path is consistent, so the imaging quality is guaranteed, and the adaptability of the microscopic imaging system is improved.
[0043] Optionally, the microscopic imaging system further includes a second slide rail 7, the first reflector 211 and the second reflector 212 are both beam splitters, a light shielding slit plate 213 is provided between the first reflector 211 and the second reflector 212, and the illumination module 3 and the imaging module 1 are slidably mounted on the second slide rail 7. Exemplarily, in a third possible implementation of the present invention, with reference to Figure 5 , by changing the composition of the reflective device group 21 in the optical path adjustment module 2, on the basis of the previous embodiment, the illumination light beam from the illumination module 3 is divided into two beams of light by a first reflector 211 and a second reflector 212 in the form of two beam splitters, the first surface of the beam splitter is semi-reflective and semi-transmissive to the light beam, and the second surface reflects the light beam completely, and a light shielding slit plate 213 is arranged between the first reflector 211 and the second reflector 212, and the other beam of light not used for imaging is absorbed by the first reflector 211, and only the light beam involved in imaging is allowed to hit the sample position of the culture dish 4. When it is necessary to image the samples at different positions in the culture dish 4, it is only necessary to move the illumination module 3 and the imaging module 1 as a whole relative to the second slide rail 7, and there is no need to adjust the separate spacing between the illumination module 3 and the imaging module 1, so as to achieve the adjustment of the imaging position. The adjustment mechanism 5 composed of the slide rail bracket, the electric push rod, etc. can be omitted, simplifying the composition of the mobile control module in the entire time difference incubator, and reducing the setting cost.
[0044] Optionally, the lighting module 3 is located beside the light path adjustment module 2 in the horizontal direction, and the reflective device group 21 includes a beam splitter 214 and a third reflector 215 arranged in parallel and spaced relation along the light path of the illumination light source 31, and a compensation glass 216 is arranged below the third reflector 215. Exemplarily, in another possible implementation of the present invention, exemplary, in a fourth possible implementation of the present invention, reference Figure 6, adjust the position of the illumination module 3, and place the illumination module 3 horizontally beside the optical path adjustment module 2. The two can be set together in the culture chamber and suspended above the culture dish 4. Further, adjust the reflective device group 21, and arrange the beam splitter 214 and the third reflector 215 in parallel and at intervals along the optical path. The horizontal light emitted by the illumination light source 31 first passes through the beam splitter 214 to achieve semi-reflection and semi-transmission, so that the light beam with half intensity is reflected to the sample on the left side of the culture dish 4. The transmitted light beam is reflected by the third reflector 215, and is compensated for the optical path by the compensation glass 216, so as to ensure that the optical paths of the two interval illumination light paths are consistent, and finally illuminate the sample on the right side of the culture dish 4, so as to realize simultaneous illumination and imaging of the two samples on the culture dish 4 through one light beam, thereby improving the imaging efficiency.
[0045] For example, in the embodiment of the present utility model, reference is made to Figure 7 Based on the fourth possible implementation method, the beam splitter 214 and the third reflector 215 can also be combined with the first and second embodiments. The beam splitter 214 and the third reflector 215 are used instead of the second reflector 212 to achieve simultaneous irradiation and imaging of two samples, thereby improving imaging efficiency.
[0046] Optionally, the microscopic imaging system further comprises a third slide rail 8, a plurality of groups of embryo placement holes 41 are arranged on the culture dish 4, each group of embryo placement holes 41 comprises a plurality of embryo placement holes 41 arranged at intervals along a first direction, the plurality of groups of embryo placement holes 41 are arranged at intervals along a second direction perpendicular to the first direction, the third slide rail 8 is arranged along the second direction, and the illumination module 3 and the imaging module 1 are slidably mounted on the third slide rail 8. Exemplarily, in an embodiment of the utility model, the culture dish 4 is arranged with a plurality of embryo placement holes 41, so that a plurality of independent samples can be placed and cultured, for example Figure 4 The culture dish 4 includes 8 embryo placement holes 41, which are divided into four groups in pairs and arranged at intervals along the second direction. Correspondingly, in the aforementioned embodiment, it is possible to realize the independent imaging of the embryo samples in the two embryo placement holes 41 in the first direction, or the simultaneous imaging, by setting the third slide rail 8 extending in the second direction, the entire lighting module 3 and the imaging module 1 can be slid as a whole in the second direction by means of a sliding motor drive, etc., so as to realize the corresponding illumination and imaging of the multiple groups of embryo placement holes 41, and further improve the practicality and imaging efficiency of the microscopic imaging system.
[0047] Optionally, the aperture of the slit plate 32 is adjustable. Within a certain range, the larger the illumination angle of the light spot at the sample on the culture dish 4 in the oblique illumination imaging, the stronger the imaging resolution. For example, in the embodiment of the utility model, a plurality of slit plates 32 with shaping slits 131 of different lengths and widths can be prepared in advance. According to different types of samples and imaging requirements, shaping slits 131 of different sizes can be used in a replacement manner to adjust the oblique illumination angle, further expanding the dynamic control range of imaging contrast and resolution.
[0048] refer to Figure 8 The present invention also provides a time difference incubator, which may include: Figures 1 to 7 The microscopic imaging system shown in the figure also includes a culture chamber a and a culture box shell b, the culture chamber a is installed above the culture box shell b and is configured to be light-transmitting with the culture box shell b, the light path adjustment module 2 is arranged in the culture chamber a, the culture dish 4 is arranged at the bottom of the culture chamber a, and the imaging module 1 and the lighting module 3 are arranged in the culture box shell b. The time difference incubator adopting this setting form integrates the lighting module 3 and the imaging module 1 which are usually arranged above the culture chamber a as a whole in the prior art into the culture box shell b, and only realizes the folding reflection of the illumination light path by additionally arranging the light path adjustment module 2 at the top of the culture chamber a, so as to greatly reduce the volume and space occupied by the time difference incubator.
[0049] Unless otherwise defined, the technical terms or scientific terms used herein shall have the usual meanings understood by persons with ordinary skills in the field to which the utility model belongs. The words "first", "second" and similar words used in the specification and claims of the utility model patent application do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, words such as "one" or "one" do not indicate a quantity limitation, but indicate the existence of at least one. Words such as "include" or "comprise" and similar words mean that the elements or objects appearing before "include" or "comprise" cover the elements or objects listed after "include" or "comprise" and are extremely equivalent, and do not exclude other elements or objects. Words such as "connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.
[0050] The above description is only an optional embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.
Claims
1. A microscopic imaging system, characterized in that: include: An imaging module (1), an optical path adjustment module (2), an illumination module (3) and a culture dish (4), The imaging module (1) is arranged below the optical path adjustment module (2), and comprises an objective lens (11), an objective lens modulation plate (12), a lens barrel lens (13), and an image collector (14) arranged at intervals in a direction away from the optical path adjustment module (2); the culture dish (4) is located between the imaging module (1) and the optical path adjustment module (2); The illumination module (3) is arranged on one side of the imaging module (1) in the horizontal direction, and comprises an illumination light source (31), a slit plate (32) and a focusing system (33) arranged at intervals in a direction close to the optical path adjustment module (2); the slit plate (32) is provided with a shaping slit that is conjugate with the objective lens modulation plate (12); A reflective device group (21) is provided in the light path adjustment module (2) for guiding the illumination light spot emitted by the illumination light source (31) so as to illuminate the culture dish (4).
2. The microscopic imaging system according to claim 1, characterized in that: The illumination module (3) is located below the light path adjustment module (2) and is arranged in parallel with the imaging module (1); the reflective device group (21) comprises a first reflector (211) and a second reflector (212) arranged at an angle interval; the first reflector (211) is located above the illumination module (3); and the second reflector (212) is located above the culture dish (4).
3. The microscopic imaging system according to claim 2, characterized in that: The microscopic imaging system further comprises an adjustment mechanism (5) and a first slide rail (6); the illumination module (3) is connected to the imaging module (1) via the adjustment mechanism (5) to achieve sliding adjustable spacing; the illumination module (3) is slidably mounted on the first slide rail (6); and the sliding direction of the illumination module (3) is parallel to the sliding direction of the imaging module (1).
4. The microscopic imaging system according to claim 2, characterized in that: The microscopic imaging system further comprises a second slide rail (7); the first reflector (211) and the second reflector (212) are both beam splitters; a light shielding slit plate (213) is provided between the first reflector (211) and the second reflector (212); and the illumination module (3) and the imaging module (1) are slidably mounted on the second slide rail (7).
5. The microscopic imaging system according to claim 1, characterized in that: The illumination module (3) is located beside the light path adjustment module (2) in the horizontal direction; the reflective device group (21) comprises a beam splitter (214) and a third reflector (215) which are arranged in parallel and spaced relation along the light path of the illumination light source (31); a compensation glass (216) is provided below the third reflector (215).
6. The microscopic imaging system according to any one of claims 1 to 5, characterized in that: The microscopic imaging system further comprises a third slide rail (8), the culture dish (4) is provided with a plurality of groups of embryo placement holes (41), each group of the embryo placement holes (41) comprises a plurality of the embryo placement holes (41) arranged at intervals along a first direction, the plurality of groups of the embryo placement holes (41) are arranged at intervals along a second direction perpendicular to the first direction, the third slide rail (8) is arranged along the second direction, and the illumination module (3) and the imaging module (1) are slidably mounted on the third slide rail (8).
7. The microscopic imaging system according to any one of claims 1 to 5, characterized in that: The light aperture of the slit plate (32) is adjustable.
8. A time-difference incubator, comprising the microscopic imaging system according to any one of claims 1 to 5, characterized in that: It also comprises a culture chamber (a) and a culture box shell (b), wherein the culture chamber (a) is installed above the culture box shell (b), the light path adjustment module (2) is arranged in the culture chamber (a), the culture dish (4) is arranged at the bottom of the culture chamber (a), and the imaging module (1) and the lighting module (3) are arranged in the culture box shell (b).
9. The time difference incubator according to claim 8, characterized in that: A plurality of the culture chambers (a) are arranged on the incubator shell (b), and the imaging module (1) and the lighting module (3) are configured to be capable of biaxial movement in a horizontal direction.