Amino acid activation device for polypeptide synthesis

Abstract

The utility model discloses an amino acid activation device for polypeptide synthesis, the amino acid activation device for polypeptide synthesis comprises a column body, a top cover, a first driving piece, a bottom plate, a plurality of stirring shafts and a driving assembly, the column body is provided with a plurality of through holes extending in the axial direction of the column body, and one end of the column body is provided with a groove; the top cover is rotationally connected to the end, provided with the groove, of the column body, and a liquid inlet communicated with one through hole is formed in the side, away from the column body, of the top cover; the bottom plate is arranged on the side, away from the top cover, of the column body, and liquid outlet flow channels communicating with the through holes are formed in the bottom plate; the stirring shaft is arranged in the through hole, the axial direction of the stirring shaft is the same as that of the column body, the stirring shaft penetrates through the bottom plate, and the stirring shaft is rotationally connected with the bottom plate and has the degree of freedom of rotating around the axial direction of the stirring shaft; the driving assembly is arranged on the side, away from the column body, of the bottom plate and connected with the multiple stirring shafts, and the driving assembly provides rotation driving force for the stirring shafts. The initial solution is continuously stirred, the mixed solution continuously flows out, and the working efficiency is higher.

Description

Technical Field

[0001] The present application relates to the technical field of polypeptide synthesis, and in particular to an amino acid activation device for polypeptide synthesis. Background Art

[0002] Peptides are a class of compounds formed by multiple amino acids connected by peptide bonds. Amino acid activation is required before peptide synthesis. Amino acid activation refers to adding a certain ratio of amino acids and activators into an activation reactor for full contact and mixing.

[0003] In the related art, the mixing of amino acids and activators in the activation reactor is carried out in batches. Only after the mixing is completed can the mixed solution be introduced into the peptide synthesis instrument for peptide synthesis, which affects the efficiency of peptide synthesis. Utility Model Content

[0004] The main purpose of the present application is to provide an amino acid activation device for peptide synthesis, which aims to solve the problem that in the related art, the mixing of amino acids and activators in the activation reactor is carried out in batches, and the mixed solution can only be passed into the peptide synthesis instrument for peptide synthesis after the mixing is completed, which affects the efficiency of peptide synthesis.

[0005] To achieve the above-mentioned purpose, the present application provides an amino acid activation device for polypeptide synthesis, which comprises a column, a top cover, a first driving member, a bottom plate, a plurality of stirring shafts and a driving assembly, wherein the column is provided with a plurality of through holes extending along its own axial direction and one end is provided with a groove, and the through holes are arranged at intervals on the periphery of the groove; the top cover is rotatably connected to the end of the column provided with the groove and has the freedom to rotate around the axial direction of the column, and a liquid inlet connected to one of the through holes is provided on the side of the top cover away from the column; the first driving member is arranged in the groove and connected to the top cover The first driving member provides a rotational driving force for the top cover; the bottom plate is arranged on the side of the column away from the top cover, and a liquid outlet channel connected to the plurality of through holes is arranged on the bottom plate; a plurality of stirring shafts correspond to the plurality of through holes one by one and are arranged in the corresponding through holes, the axial direction of the stirring shaft is the same as the axial direction of the column and passes through the bottom plate, the stirring shaft is rotatably connected to the bottom plate and has the freedom to rotate around its own axial direction; the driving component is arranged on the side of the bottom plate away from the column and is connected to the plurality of stirring shafts, and the driving component provides a rotational driving force for each of the stirring shafts.

[0006] Optionally, the liquid outlet channel includes a liquid outlet, multiple channels and multiple solenoid valves, wherein the liquid outlet is arranged on the side of the bottom plate away from the column; the multiple channels are connected to the through holes one by one, and the channels are all connected to the liquid outlet; the multiple solenoid valves correspond to the multiple channels one by one and are arranged in the corresponding channels.

[0007] Optionally, the driving assembly includes a second driving member, a driving sprocket, a plurality of driven sprockets and a chain, the second driving member is fixed to the base plate; the driving sprocket is connected to the second driving member and rotates around its own axis in response to the driving force provided by the second driving member; the plurality of driven sprockets correspond one by one to the plurality of agitator shafts and are sleeved on the outer periphery of the corresponding agitator shafts; the chain connects the plurality of driven sprockets and the driving sprocket.

[0008] Optionally, there is a gap between the driven sprocket and the corresponding stirring shaft; the amino acid activation device for polypeptide synthesis also includes multiple sleeves and multiple transmission components, the multiple sleeves correspond one-to-one to the multiple driven sprockets and are fixed to the side of the corresponding driven sprocket facing the bottom plate, the axial direction of the sleeve is the same as the axial direction of the corresponding driven sprocket and is rotatably connected to the bottom plate, and the sleeve has the freedom to rotate around its own axial direction; the multiple transmission components are connected one-to-one to the multiple stirring shafts and have the freedom to move along the axial direction of the stirring shaft, and the transmission component is inserted into the gap between the driven sprocket and the corresponding stirring shaft in response to the gravity of the liquid in the through hole, so that the stirring shaft is driven to rotate when the driven sprocket rotates.

[0009] Optionally, the stirring shaft has a cavity therein; the transmission assembly includes a sliding shaft, a pressure-bearing part, an extrusion part and a spring, the sliding shaft slidingly fits in the cavity and passes through the bottom plate; the pressure-bearing part is connected to one end of the sliding shaft located in the through hole, and the pressure-bearing part pushes the sliding shaft away from the top cover in response to the gravity of the liquid in the through hole; the extrusion part is connected to one end of the sliding shaft located outside the through hole, and the extrusion part cooperates with the driven sprocket so that the stirring shaft is driven to rotate when the driven sprocket rotates; the spring extends along the axial direction of the stirring shaft and is located in the cavity, and the two ends of the spring are respectively connected to the sliding shaft and the stirring shaft.

[0010] Optionally, the pressure-bearing part includes a pressure-bearing ring and a first connecting shaft, the pressure-bearing ring is sleeved on the outer circumference of the stirring shaft; the first connecting shaft is located on the inner circumference of the pressure-bearing ring and has two ends respectively connecting the pressure-bearing ring and the sliding shaft; wherein a first through groove corresponding to the first connecting shaft is provided on the stirring shaft, the first connecting shaft passes through the first through groove and has the freedom to move along the axial direction of the stirring shaft.

[0011] Optionally, the inner circumference of the pressure-bearing ring is in contact with the stirring shaft; and the outer circumference of the pressure-bearing ring is in contact with the side wall of the through hole.

[0012] Optionally, the extrusion portion includes an extrusion ring and a second connecting shaft, the extrusion ring is sleeved on the outer circumference of the stirring shaft and is located between the driven sprocket and the base plate; the second connecting shaft is located on the inner circumference of the extrusion ring and has two ends respectively connected to the extrusion ring and the sliding shaft; wherein a second through groove corresponding to the second connecting shaft is provided on the stirring shaft, the second connecting shaft passes through the second through groove and has the freedom to move along the axial direction of the stirring shaft.

[0013] Optionally, the outer diameter of the extrusion ring gradually decreases in the direction away from the base plate; the inner diameter of the driven sprocket gradually decreases in the direction away from the base plate; wherein the taper of the outer periphery of the extrusion ring is the same as the taper of the inner periphery of the driven sprocket.

[0014] Optionally, the amino acid activation device for polypeptide synthesis further comprises a plurality of supporting legs, and the plurality of supporting legs are all arranged on a side of the bottom plate away from the column.

[0015] An amino acid activation device for polypeptide synthesis proposed in an embodiment of the present application, wherein an initial solution of amino acids and an activator is introduced into a liquid inlet, and when the initial solution enters the corresponding through hole and fills the corresponding through hole, the first driving member rotates to drive the top cover to rotate, so that the initial solution introduced subsequently enters other through holes, and at the same time, the driving component drives each stirring shaft to rotate around its own axis, and the stirring shaft stirs the initial solution in the corresponding through hole to fully mix it to form a mixed liquid, and then the mixed liquid flows out of the amino acid activation device through a liquid outlet channel and flows to a subsequent polypeptide synthesis instrument; during the process, the initial solution is circulated and added to multiple through holes, and the mixed liquids stirred in the multiple through holes are circulated and flow out of the liquid outlet channel in sequence, that is, the initial solution is continuously stirred, and the mixed liquid flows out continuously, which has higher working efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A schematic diagram of the overall structure of an amino acid activation device for polypeptide synthesis provided in an embodiment of the present application;

[0017] Figure 2 for Figure 1 A schematic diagram of the structural disassembly of the embodiment in FIG.

[0018] Figure 3 A schematic diagram of the structure of the bottom plate of an amino acid activation device for polypeptide synthesis provided in an embodiment of the present application;

[0019] Figure 4 for Figure 3 Another perspective structural diagram of the embodiment;

[0020] Figure 5 for Figure 3 A schematic diagram of the structural disassembly of the embodiment in FIG.

[0021] Figure 6 A schematic diagram of the bottom plate structure of an amino acid activation device for polypeptide synthesis provided in an embodiment of the present application;

[0022] Figure 7 A schematic diagram of the structure of a transmission component of an amino acid activation device for polypeptide synthesis provided in an embodiment of the present application;

[0023] Figure 8 for Figure 7 A schematic diagram of the structural disassembly of the embodiment in FIG.

[0024] Fig. 9 This is a schematic diagram of the structural breakdown of the sliding axis of an amino acid activation device for polypeptide synthesis provided in an embodiment of the present application.

[0025] In the figure: 1. cylinder; 11. through hole; 12. groove; 2. top cover; 21. liquid inlet; 3. first driving member; 4. bottom plate; 41. liquid outlet; 42. flow channel; 43. solenoid valve; 44. sleeve; 45. supporting leg; 5. stirring shaft; 51. first through groove; 52. second through groove; 61. second driving member; 62. driving sprocket; 63. driven sprocket; 64. chain; 71. sliding shaft; 72. pressure-bearing part; 721. pressure-bearing ring; 722. first connecting shaft; 73. extrusion part; 731. extrusion ring; 732. second connecting shaft; 74. spring.

[0026] The realization of the purpose, functional features and advantages of this application will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0028] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0029] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the utility model.

[0031] refer to Figures 1 to 9 The embodiment of the present application provides an amino acid activation device for polypeptide synthesis, which may include a column 1, a top cover 2, a first driving member 3, a bottom plate 4, a plurality of stirring shafts 5 and a driving assembly, wherein the column 1 is provided with a plurality of through holes 11 extending along its own axial direction and a groove 12 is provided at one end, and the through holes 11 are arranged at intervals on the periphery of the groove 12; the top cover 2 is rotatably connected to one end of the column 1 provided with the groove 12 and has the freedom to rotate around the axial direction of the column 1, and a liquid inlet 21 connected to a through hole 11 is provided on the side of the top cover 2 away from the column 1; the first driving member 3 is provided The bottom plate 4 is arranged on the side of the column 1 away from the top cover 2, and a liquid outlet channel connected with a plurality of through holes 11 is arranged on the bottom plate 4; a plurality of stirring shafts 5 correspond to the plurality of through holes 11 one by one and are arranged in the corresponding through holes 11, the axial direction of the stirring shaft 5 is the same as the axial direction of the column 1 and passes through the bottom plate 4, the stirring shaft 5 is rotatably connected to the bottom plate 4 and has the freedom to rotate around its own axial direction; the driving component is arranged on the side of the bottom plate 4 away from the column 1 and is connected to the plurality of stirring shafts 5, and the driving component provides a rotational driving force for each stirring shaft 5.

[0032] An amino acid activation device for polypeptide synthesis proposed in an embodiment of the present application is a device in which an initial solution of amino acids and an activator is introduced into a liquid inlet 21. When the initial solution enters the corresponding through hole 11 and fills the corresponding through hole 11, the first driving member 3 rotates to drive the top cover 2 to rotate, so that the initial solution introduced subsequently enters other through holes 11. At the same time, the driving component drives each stirring shaft 5 to rotate around its own axis. The stirring shaft 5 stirs the initial solution in the corresponding through hole 11 to fully mix it to form a mixed solution, and then the mixed solution flows out of the amino acid activation device through a liquid outlet channel and flows to a subsequent polypeptide synthesis instrument. During the process, the initial solution is circulated and added into multiple through holes 11, and the mixed solutions stirred in the multiple through holes 11 are circulated and flow out from the liquid outlet channel in sequence, that is, the initial solution is continuously stirred, and the mixed solution flows out continuously, which has higher working efficiency.

[0033] Among them, there are four through holes 11, and the four through holes 11 are evenly spaced and arranged in the circumferential direction; the bottom plate 4 and the top cover 2 respectively block the two ends of the through holes 11 to form a chamber for mixing amino acids and activators. After mixing, the mixed liquid flows out from the liquid outlet channel on the bottom plate 4.

[0034] It should be noted that the first driving member 3 may be a first motor, the output shaft of the first motor is fixed to the top cover 2 to drive the top cover 2 to rotate, and the first motor may be connected to an external power source.

[0035] Furthermore, the amino acid activation device for polypeptide synthesis may also include a plurality of support legs 45 , and the plurality of support legs 45 are all arranged on a side of the bottom plate 4 away from the column 1 , so that the plurality of support legs 45 can support the device.

[0036] refer to Figure 6 In an exemplary embodiment, the liquid outlet channel may include a liquid outlet 41, a plurality of channels 42 and a plurality of solenoid valves 43, wherein the liquid outlet 41 is arranged on a side of the bottom plate 4 away from the column 1; the plurality of channels 42 are connected to the plurality of through holes 11 in a one-to-one correspondence, and the channels 42 are all connected to the liquid outlet 41; the plurality of solenoid valves 43 correspond to the plurality of channels 42 in a one-to-one correspondence and are arranged in the corresponding channels 42.

[0037] Specifically, Figure 6 As shown, when the amino acid and the activator in a certain through hole 11 are mixed, the corresponding solenoid valve 43 is opened, and the mixed liquid in the through hole 11 can flow into the liquid outlet 41 through the corresponding flow channel 42 .

[0038] Furthermore, the flow channel 42 on the bottom plate 4 may be connected to the corresponding through hole 11 through a pipeline, and the connection area between the pipeline and the through hole 11 may be located at the end of the through hole 11 or at the side wall of the through hole 11 .

[0039] refer to Figure 5In an exemplary embodiment, the driving assembly may include a second driving member 61, a driving sprocket 62, a plurality of driven sprockets 63 and a chain 64, wherein the second driving member 61 is fixed to the base plate 4; the driving sprocket 62 is connected to the second driving member 61 and rotates around its own axis in response to the driving force provided by the second driving member 61; the plurality of driven sprockets 63 correspond one by one to the plurality of stirring shafts 5 and are sleeved on the outer periphery of the corresponding stirring shafts 5; the chain 64 connects the plurality of driven sprockets 63 and the driving sprocket 62.

[0040] Specifically, the second driving member 61 can be a second motor, which is connected to an external power supply. The driving sprocket 62 is sleeved on the outer periphery of the output shaft of the second motor. The second motor drives the driving sprocket 62 to rotate. The driving sprocket 62 drives multiple driven sprockets 63 to rotate through the chain 64. The driven sprocket 63 drives the corresponding stirring shaft 5 to rotate. In this way, each stirring shaft 5 can stir the amino acid and activator in the corresponding through hole 11 to fully mix them.

[0041] refer to Figure 7 In an exemplary embodiment, there is a gap between the driven sprocket 63 and the corresponding stirring shaft 5; the amino acid activation device for polypeptide synthesis may also include multiple sleeves 44 and multiple transmission components, the multiple sleeves 44 correspond to the multiple driven sprockets 63 one by one and are fixed to the side of the corresponding driven sprocket 63 facing the bottom plate 4, the axial direction of the sleeve 44 is the same as the axial direction of the corresponding driven sprocket 63 and is rotatably connected to the bottom plate 4, and the sleeve 44 has the freedom to rotate around its own axial direction; the multiple transmission components are connected to the multiple stirring shafts 5 one by one and have the freedom to move along the axial direction of the stirring shaft 5, and the transmission components are inserted into the gap between the driven sprocket 63 and the corresponding stirring shaft 5 in response to the gravity of the liquid in the through hole 11, so that the stirring shaft 5 is driven to rotate when the driven sprocket 63 rotates.

[0042] Specifically, in the direction of gravity, when the device is used, the top cover 2 is located above the bottom plate 4. When the amino acid and the activator are introduced into the through hole 11, the gravity of the liquid in the through hole 11 continues to increase until the corresponding transmission component is inserted into the gap between the corresponding driven sprocket 63 and the corresponding stirring shaft 5. At this time, when the driven sprocket 63 rotates, it can drive the corresponding stirring shaft 5 to rotate to stir the amino acid and the activator; in this way, when the second motor continues to work, it drives each driven sprocket 63 to rotate, and each driven sprocket 63 is connected to the bottom plate 4 through the sleeve 44 for rotation. If the amino acid and the activator are not introduced into the through hole 11, the corresponding driven sprocket 63 will not drive the stirring shaft 5 to rotate; if the amino acid and the activator are introduced into the through hole 11, the corresponding driven sprocket 63 will drive the stirring shaft 5 to rotate through the transmission component to mix the amino acid and the activator in the corresponding through hole 11, so that the device is more energy-efficient when used.

[0043] refer to Figure 7 and Figure 8In an exemplary embodiment, a cavity is provided in the stirring shaft 5; the transmission assembly may include a sliding shaft 71, a pressure-bearing portion 72, an extrusion portion 73 and a spring 74, the sliding shaft 71 is slidably fitted in the cavity and passes through the bottom plate 4; the pressure-bearing portion 72 is connected to one end of the sliding shaft 71 located in the through hole 11, and the pressure-bearing portion 72 pushes the sliding shaft 71 away from the top cover 2 in response to the gravity of the liquid in the through hole 11; the extrusion portion 73 is connected to one end of the sliding shaft 71 located outside the through hole 11, and the extrusion portion 73 cooperates with the driven sprocket 63 so that the stirring shaft 5 is driven to rotate when the driven sprocket 63 rotates; the spring 74 extends along the axial direction of the stirring shaft 5 and is located in the cavity, and the two ends of the spring 74 are respectively connected to the sliding shaft 71 and the stirring shaft 5.

[0044] Specifically, when the amino acid and the activator are introduced into the through hole 11, the gravity of the liquid squeezes the pressure-bearing part 72 so that the pressure-bearing part 72 moves along the axial direction of the stirring shaft 5 and approaches the bottom plate 4. The pressure-bearing part 72 drives the sliding shaft 71 to slide in the cavity. The sliding shaft 71 further drives the extrusion part 73 to approach the corresponding driven sprocket 63 until the extrusion part 73 cooperates with the corresponding driven sprocket 63, so that when the driven sprocket 63 rotates, it drives the corresponding stirring shaft 5 to rotate, so that the amino acid activator can be mixed.

[0045] Furthermore, when the mixing is completed, the corresponding solenoid valve 43 is opened, and the mixed liquid is discharged from the flow channel 42 through the pipeline, and finally flows into the instrument used in the subsequent steps through the liquid outlet 41. During the process, the pressure on the pressure-bearing part 72 gradually decreases until the spring 74 pushes the sliding shaft 71 to move, so that the pressure-bearing part 72 is away from the bottom plate 4, and the extrusion part 73 is separated from the corresponding driven sprocket 63. In this way, the corresponding driven sprocket 63 cannot drive the stirring shaft 5 to rotate, that is, when there is no liquid or less liquid in the corresponding through hole 11, the corresponding stirring shaft 5 no longer rotates, thereby finally achieving the effect of energy saving.

[0046] refer to Fig. 9 In an exemplary embodiment, the pressure-bearing portion 72 may include a pressure-bearing ring 721 and a first connecting shaft 722, wherein the pressure-bearing ring 721 is sleeved on the outer periphery of the stirring shaft 5; the first connecting shaft 722 is located on the inner periphery of the pressure-bearing ring 721 and the two ends thereof are respectively connected to the pressure-bearing ring 721 and the sliding shaft 71; wherein a first through groove 51 corresponding to the first connecting shaft 722 is provided on the stirring shaft 5, the first connecting shaft 722 passes through the first through groove 51 and has the freedom to move along the axial direction of the stirring shaft 5; the inner periphery of the pressure-bearing ring 721 is in contact with the stirring shaft 5; the outer periphery of the pressure-bearing ring 721 is in contact with the side wall of the through hole 11.

[0047] It should be understood that when the amino acid and the activator are introduced into the through hole 11, the liquid pushes the pressure ring 721 to move axially along the stirring shaft 5 and approach the bottom plate 4. The pressure ring 721 drives the sliding shaft 71 to move through the first connecting shaft 722. During the process, the first connecting shaft 722 moves in the first through groove 51.

[0048] Furthermore, the inner circumference of the pressure ring 721 fits with the stirring shaft 5, and the outer circumference of the pressure ring 721 fits with the side wall of the through hole 11, so that the amino acid and the activator in the through hole 11 are located between the pressure ring 721 and the top cover 2, and the gravity of the liquid can push the pressure ring 721 to move, ensuring that the pressure ring 721 can be used normally; at this time, the flow channel 42 on the bottom plate 4 is connected to the corresponding through hole 11 through a pipeline, and the connection area is located at the side wall of the through hole 11, so as to ensure that the pressure ring 721 and the flow channel 42 do not interfere with each other.

[0049] refer to Fig. 9 In an exemplary embodiment, the extrusion portion 73 may include an extrusion ring 731 and a second connecting shaft 732, the extrusion ring 731 is sleeved on the outer periphery of the stirring shaft 5 and is located between the driven sprocket 63 and the bottom plate 4; the second connecting shaft 732 is located on the inner periphery of the extrusion ring 731 and the two ends are respectively connected to the extrusion ring 731 and the sliding shaft 71; wherein, a second through groove 52 corresponding to the second connecting shaft 732 is provided on the stirring shaft 5, and the second connecting shaft 732 passes through the second through groove 52 and has the freedom to move along the axial direction of the stirring shaft 5.

[0050] When the driven sprocket 63 is in the state of being moved, the second end of the cam 732 is moved, and the second end of the cam 732 is moved, so that ...

[0051] refer to Figure 8 and Fig. 9 In an exemplary embodiment, the outer diameter of the extrusion ring 731 gradually decreases in the direction away from the base plate 4; the inner diameter of the driven sprocket 63 gradually decreases in the direction away from the base plate 4; wherein the taper of the outer periphery of the extrusion ring 731 is the same as the taper of the inner periphery of the driven sprocket 63.

[0052] Specifically, the outer periphery of the extrusion ring 731 and the inner periphery of the driven sprocket 63 are both inverted frustum shapes, and the taper of the outer periphery of the extrusion ring 731 is the same as the taper of the inner periphery of the driven sprocket 63. In this way, when the extrusion ring 731 approaches and contacts the driven sprocket 63, the contact area between the extrusion ring 731 and the driven sprocket 63 is larger. Under the premise that the pressure of the extrusion ring 731 squeezing the driven sprocket 63 remains unchanged, the larger the contact area, the greater the friction force, so that the transmission between the driven sprocket 63 and the extrusion ring 731 can be more stable.

[0053] The above are only preferred embodiments of the present application, and are not intended to limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. An amino acid activation device for polypeptide synthesis, characterized in that: include: A column (1) is provided with a plurality of through holes (11) extending along its axial direction and a groove (12) is provided at one end, wherein the through holes (11) are arranged at intervals on the periphery of the groove (12); A top cover (2) is rotatably connected to one end of the column (1) provided with the groove (12) and has the freedom to rotate around the axial direction of the column (1); a liquid inlet (21) communicating with the through hole (11) is provided on a side of the top cover (2) facing away from the column (1); A first driving member (3) is disposed in the groove (12) and connected to the top cover (2), wherein the first driving member (3) provides a rotational driving force for the top cover (2); A bottom plate (4) is arranged on a side of the column (1) away from the top cover (2), and a liquid outlet channel connected to the plurality of through holes (11) is arranged on the bottom plate (4); A plurality of stirring shafts (5) corresponding to the plurality of through holes (11) one by one and arranged in the corresponding through holes (11); the axial direction of the stirring shaft (5) is the same as the axial direction of the column (1) and passes through the bottom plate (4); the stirring shaft (5) is rotatably connected to the bottom plate (4) and has the freedom to rotate around its own axial direction; A driving assembly is arranged on a side of the bottom plate (4) away from the column (1) and connected to the plurality of stirring shafts (5), wherein the driving assembly provides a rotational driving force for each stirring shaft (5).

2. The amino acid activation device for polypeptide synthesis according to claim 1, characterized in that: The liquid outlet channel comprises: A liquid outlet (41) is arranged on a side of the bottom plate (4) facing away from the column (1); A plurality of flow channels (42) are connected to the plurality of through holes (11) in a one-to-one correspondence, and the flow channels (42) are all connected to the liquid outlet (41); A plurality of electromagnetic valves (43) correspond one-to-one to the plurality of flow channels (42) and are arranged in the corresponding flow channels (42).

3. The amino acid activation device for polypeptide synthesis according to claim 1, characterized in that: The drive assembly comprises: A second driving member (61) is fixed to the base plate (4); A driving sprocket (62) connected to the second driving member (61) and axially rotating in response to a driving force provided by the second driving member (61); A plurality of driven sprockets (63), corresponding one to one with the plurality of stirring shafts (5) and sleeved on the outer circumference of the corresponding stirring shafts (5); A chain (64) connects a plurality of driven sprocket wheels (63) and a driving sprocket wheel (62).

4. The amino acid activation device for polypeptide synthesis according to claim 3, characterized in that: There is a gap between the driven sprocket (63) and the corresponding stirring shaft (5); the amino acid activation device for polypeptide synthesis also includes: A plurality of sleeves (44) corresponding one to the plurality of driven sprockets (63) and fixed to the side of the corresponding driven sprocket (63) facing the bottom plate (4); the axial direction of the sleeve (44) is the same as the axial direction of the corresponding driven sprocket (63) and is rotatably connected to the bottom plate (4); and the sleeve (44) has the freedom to rotate around its own axial direction; A plurality of transmission components are connected to the plurality of stirring shafts (5) in a one-to-one correspondence and have the freedom to move along the axial direction of the stirring shaft (5). The transmission components are inserted into the gap between the driven sprocket (63) and the corresponding stirring shaft (5) in response to the gravity of the liquid in the through hole (11), so that the stirring shaft (5) is driven to rotate when the driven sprocket (63) rotates.

5. The amino acid activation device for polypeptide synthesis according to claim 4, characterized in that: The stirring shaft (5) has a cavity therein; the transmission assembly comprises: A sliding shaft (71) slidingly fitted in the cavity and passing through the bottom plate (4); a pressure-bearing portion (72) connected to one end of the sliding shaft (71) located in the through hole (11), wherein the pressure-bearing portion (72) pushes the sliding shaft (71) away from the top cover (2) in response to the gravity of the liquid in the through hole (11); An extrusion portion (73) is connected to one end of the sliding shaft (71) located outside the through hole (11), and the extrusion portion (73) cooperates with the driven sprocket (63) so that when the driven sprocket (63) rotates, the stirring shaft (5) is driven to rotate; A spring (74) extends along the axial direction of the stirring shaft (5) and is located in the cavity, and two ends of the spring (74) are respectively connected to the sliding shaft (71) and the stirring shaft (5).

6. The amino acid activation device for polypeptide synthesis according to claim 5, characterized in that: The pressure-bearing portion (72) comprises: A pressure ring (721) is sleeved on the outer circumference of the stirring shaft (5); A first connecting shaft (722), located on the inner periphery of the pressure ring (721) and having two ends respectively connected to the pressure ring (721) and the sliding shaft (71); Wherein, a first through groove (51) corresponding to the first connecting shaft (722) is provided on the stirring shaft (5); the first connecting shaft (722) passes through the first through groove (51) and has the freedom to move along the axial direction of the stirring shaft (5).

7. The amino acid activation device for polypeptide synthesis according to claim 6, characterized in that: The inner circumference of the pressure-bearing ring (721) is in contact with the stirring shaft (5); The outer periphery of the pressure-bearing ring (721) is in contact with the side wall of the through hole (11).

8. The amino acid activation device for polypeptide synthesis according to claim 5, characterized in that: The extrusion portion (73) comprises: An extrusion ring (731) is sleeved on the outer circumference of the stirring shaft (5) and is located between the driven sprocket (63) and the bottom plate (4); A second connecting shaft (732), located on the inner periphery of the extrusion ring (731) and having two ends respectively connected to the extrusion ring (731) and the sliding shaft (71); Wherein, a second through groove (52) corresponding to the second connecting shaft (732) is provided on the stirring shaft (5), and the second connecting shaft (732) passes through the second through groove (52) and has the freedom to move along the axial direction of the stirring shaft (5).

9. The amino acid activation device for polypeptide synthesis according to claim 8, characterized in that: The outer diameter of the extrusion ring (731) gradually decreases in a direction away from the bottom plate (4); The inner diameter of the driven sprocket (63) gradually decreases in a direction away from the bottom plate (4); The taper of the outer circumference of the extrusion ring (731) is the same as the taper of the inner circumference of the driven sprocket (63).

10. The amino acid activation device for polypeptide synthesis according to claim 1, characterized in that: The amino acid activation device for polypeptide synthesis also includes: A plurality of supporting legs (45) are arranged on a side of the base plate (4) facing away from the column (1).

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