Liquid strain inoculation gun

Abstract

The liquid strain inoculation gun comprises an air cylinder, a piston and an inoculation needle, a first channel is arranged at the front end of the air cylinder, the front end of the air cylinder is connected with a connector, a second channel connected with the first channel is arranged in the connector, the connector is provided with a liquid inlet and a liquid outlet which are communicated with the second channel, the liquid inlet is provided with a first one-way valve, and the liquid outlet is provided with a second one-way valve. The one-way valve II is connected with an inoculating needle; a gun handle is arranged behind the air cylinder and provided with a driving mechanism used for pushing and pulling the piston rod, an adjusting knob is arranged between the air cylinder and the gun handle, and the adjusting knob is in threaded connection with an adjusting lead screw. According to the utility model, strains can be quickly and accurately inoculated into a culture medium, the inoculation efficiency is improved, the inoculation amount can be accurately controlled, and the device can also be applied to various experiments and production scenes of liquid culture fungi, and has stronger adaptability.

Description

Technical Field

[0001] The utility model relates to the technical field of edible fungus cultivation, in particular to a liquid fungus seed inoculation gun. Background Art

[0002] At present, edible fungi cultivation and inoculation are mainly carried out through liquid inoculation. Inoculation is an indispensable step in the production process of edible fungi cultivation. Traditional inoculation adopts manual inoculation, which has problems such as low inoculation efficiency, poor accuracy, and high labor intensity. The liquid culture inoculation gun is a kind of inoculation equipment for fungi cultured in liquid. In recent years, with the development of biotechnology, the research on liquid culture inoculation guns has gradually attracted attention. At present, liquid culture inoculation guns are generally divided into pneumatic inoculators and electric inoculators. The pneumatic inoculator is based on the pneumatic principle. Compared with manual inoculation, it can improve the inoculation efficiency, but the accuracy still needs to be improved; the electric inoculator is driven by an electric motor. Compared with the pneumatic inoculator, it has higher accuracy, but the equipment cost is higher. Utility Model Content

[0003] The technical problem to be solved by the utility model is to overcome the defects of the above-mentioned technology and provide a liquid strain inoculation gun to solve the problems of low inoculation efficiency, poor inoculation accuracy, high contamination risk, complicated operation, etc. in the prior art.

[0004] In order to solve the above technical problems, the technical solution provided by the utility model is a liquid bacteria inoculation gun: comprising an air cylinder, a piston, and an inoculation needle, the piston is sealingly and slidably connected to the air cylinder, a channel 1 is provided at the front end of the air cylinder, a joint is connected to the front end of the air cylinder, a channel 2 connected to the channel 1 is provided in the joint, a liquid inlet and a liquid outlet connected to the channel 2 are provided at the joint, a one-way valve 1 is provided at the liquid inlet, a one-way valve 2 is provided at the liquid outlet, and the one-way valve 2 is connected to the inoculation needle; the piston is fixedly connected with a piston rod, a gun handle is provided at the rear of the air cylinder, the gun handle is provided with a driving mechanism for pushing and pulling the piston rod, an adjusting knob is provided between the air cylinder and the gun handle, an adjusting screw is threadedly connected to the adjusting knob, a through hole is provided in the middle of the adjusting screw for the piston rod to pass through, a through hole is provided at the rear end of the air cylinder for the adjusting screw to pass through, the gun handle is provided with a through hole for the adjusting screw and the piston rod to pass through, the adjusting screw is slidably connected to the gun handle, and the thickness of the adjusting knob is matched with the spacing between the air cylinder and the gun handle.

[0005] Furthermore, the driving mechanism includes a handle, a U-shaped bracket fixed to the upper end of the handle, and a U-shaped plate rotatably connected to the U-shaped bracket, the two wing rods of the U-shaped bracket are rotatably connected to both sides of the gun handle, the two wing plates of the U-shaped plate are rotatably connected to the two wing rods of the U-shaped bracket respectively, the web of the U-shaped plate is provided with a through hole with a diameter larger than the diameter of the piston rod, the rear end portion of the piston rod passes through the through hole provided with the U-shaped plate and the rear end portion is provided with a thread, and the rear end portion of the piston rod is threadedly connected to a pressure plate.

[0006] Furthermore, an O-shaped groove is provided on the connecting end surface of the gas cylinder connected to the joint, and an O-shaped sealing ring is provided in the O-shaped groove.

[0007] Compared with the prior art, the advantages of the utility model are: the bacteria can be inoculated into the culture medium quickly and accurately, thus improving the inoculation efficiency and shortening the time; the inoculation amount can be accurately controlled, thus avoiding errors that may occur during manual inoculation and ensuring the accuracy of the results; aseptic operation can be achieved, thus effectively reducing the risk of contamination during the inoculation process and improving the success rate; the liquid bacteria inoculation gun is easy to operate, thus reducing the workload of the staff and improving the work efficiency; it can also be applied to various liquid culture fungi experiments and production scenarios and has strong adaptability. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] Figure 1 The utility model is a cross-sectional structural schematic diagram of a liquid bacterial strain inoculation gun.

[0009] Figure 2 This is a three-dimensional structure diagram of a liquid bacterial inoculation gun of the utility model. Figure 1 .

[0010] Figure 3 This is a three-dimensional structure diagram of a liquid bacterial inoculation gun of the utility model. Figure 2 .

[0011] As shown in the figure:

[0012] 1. Air cylinder, 2. Piston, 3. Inoculation needle, 4. Channel 1, 5. Connector, 6. Channel 2, 7. One-way valve 1, 8. One-way valve 2, 9. Piston rod, 10. Gun handle, 11. Adjustment knob, 12. Adjustment screw, 13. Handle, 14. U-shaped bracket, 15. U-shaped plate, 16. Connecting pipe, 17. O-ring. DETAILED DESCRIPTION

[0013] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0014] Embodiment 1, in combination with Figure 1-3 A liquid bacterial inoculation gun comprises a cylinder 1, a piston 2, and an inoculation needle 3, wherein the piston 2 is sealingly and slidably connected to the cylinder 1, a channel 1 4 is provided at the front end of the cylinder 1, a joint 5 is connected to the front end of the cylinder 1, a channel 2 6 connected to the channel 1 4 is provided in the joint 5, a liquid inlet and a liquid outlet communicated with the channel 2 6 are provided in the joint 5, a one-way valve 1 7 is provided at the liquid inlet, a one-way valve 2 8 is provided at the liquid outlet, and the one-way valve 2 8 is connected to the inoculation needle 3; the piston 2 is fixedly connected to a piston rod 9, and a gun handle 1 is provided at the rear of the cylinder 1 0, the gun handle 10 is provided with a driving mechanism for pushing and pulling the piston rod 9, an adjusting knob 11 is provided between the gas cylinder 1 and the gun handle 10, the adjusting knob 11 is threadedly connected with an adjusting screw 12, the middle of the adjusting screw 12 is provided with a through hole for the piston rod 9 to pass through, the rear end of the gas cylinder 1 is provided with a through hole for the adjusting screw 12 to pass through, the gun handle 10 is provided with a through hole for the adjusting screw 12 and the piston rod 9 to pass through, the adjusting screw 12 is slidably connected to the gun handle 10, and the thickness of the adjusting knob 11 is adapted to the distance between the gas cylinder 1 and the gun handle 10. The one-way valve 17 is provided with a connecting pipe 16.

[0015] When in use, based on the principle of pressure difference, the bacterial liquid bottle containing bacterial liquid is connected to the connecting pipe 16 through a hose, and the piston rod 9 is pushed and pulled through the driving mechanism, and then the piston 2 is pushed and pulled. When the piston 2 moves backward, the pressure in the cylinder 1 decreases, and the bacterial liquid enters the cylinder 1 through the one-way valve 1 7. When the piston 2 moves forward, the pressure in the cylinder 1 increases, and the bacterial liquid in the cylinder 1 is sprayed out through the one-way valve 2 8 and the inoculation needle 3. The sharp needle tip of the inoculation needle 3 can easily penetrate the solid culture medium, so that the bacterial liquid can be accurately injected into the target part. Among them, by rotating the adjustment knob 11, the adjustment screw 12 is moved, and the distance that the adjustment screw 12 extends into the cylinder 1 is changed, so as to adjust the maximum position that the piston 2 can move backward, and then adjust the maximum volume of the bacterial liquid that can be inhaled, and accurately control the inoculation amount, avoid the errors that may occur during the manual inoculation process, and ensure the accuracy of the results.

[0016] The driving mechanism includes a handle 13, a U-shaped bracket 14 fixedly connected to the upper end of the handle 13, and a U-shaped plate 15 rotatably connected to the U-shaped bracket 14. The two wing rods of the U-shaped bracket 14 are rotatably connected to the two sides of the gun handle 10. The two wing plates of the U-shaped plate 15 are rotatably connected to the two wing rods of the U-shaped bracket 14 respectively. The web of the U-shaped plate 15 is provided with a through hole with a diameter larger than the diameter of the piston rod 9. The rear end of the piston rod 9 passes through the through hole provided with the U-shaped plate 15 and its rear end is provided with a thread. The rear end of the piston rod 9 is threadedly connected with a pressure plate. By pressing the handle 13 by hand, the U-shaped plate 15 is driven to move backward. After the web of the U-shaped plate 15 contacts the pressure plate, the piston rod 9 is driven to move backward, so that the piston 2 moves backward and the bacterial liquid is sucked in. When the pressure plate is pressed, the piston rod 9 can be pushed to move forward, so that the piston 2 moves forward and the bacterial liquid is discharged.

[0017] The inoculation needle 3 is threadedly connected to the one-way valve 8, which is convenient for installation and disassembly.

[0018] The joint 5 is threadedly connected to the gas cylinder 1, and is easy to install and disassemble.

[0019] The connecting end surface of the gas cylinder 1 connected to the joint 5 is provided with an O-shaped groove, and an O-shaped sealing ring 17 is arranged in the O-shaped groove to increase the sealing performance and avoid leakage.

[0020] In the description of the embodiments of the present utility model, it should be noted that if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate an orientation or position relationship based on the orientation or position relationship shown in the accompanying drawings, or the orientation or position relationship in which the utility model product is usually placed when in use, it is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0021] In addition, the terms "horizontal", "vertical", "overhanging" and the like do not mean that the components must be absolutely horizontal or overhanging, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0022] In the description of the embodiments of the present invention, "multiple" means at least 2.

[0023] In the description of the embodiments of the present utility model, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0024] The above description of the utility model and its implementation methods is not restrictive. The drawings show only one implementation method of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.

Claims

1. A liquid bacterial inoculation gun, comprising a gas cylinder (1), a piston (2), and an inoculation needle (3), wherein the piston (2) is sealingly and slidably connected to the gas cylinder (1), characterized in that: The front end of the gas cylinder (1) is provided with a channel 1 (4), the front end of the gas cylinder (1) is connected to a joint (5), the joint (5) is provided with a channel 2 (6) connected to the channel 1 (4), the joint (5) is provided with a liquid inlet and a liquid outlet communicated with the channel 2 (6), the liquid inlet is provided with a one-way valve 1 (7), the liquid outlet is provided with a one-way valve 2 (8), and the one-way valve 2 (8) is connected to an inoculation needle (3); The piston (2) is fixedly connected with a piston rod (9); a gun handle (10) is arranged at the rear of the gas cylinder (1); the gun handle (10) is provided with a driving mechanism for pushing and pulling the piston rod (9); an adjusting knob (11) is arranged between the gas cylinder (1) and the gun handle (10); the adjusting knob (11) is threadedly connected with an adjusting screw (12); a through hole for the piston rod (9) to pass through is arranged in the middle of the adjusting screw (12); a through hole for the adjusting screw (12) to pass through is arranged at the rear end of the gas cylinder (1); the gun handle (10) is provided with through holes for the adjusting screw (12) and the piston rod (9) to pass through; the adjusting screw (12) is slidably connected to the gun handle (10); the thickness of the adjusting knob (11) is adapted to the distance between the gas cylinder (1) and the gun handle (10).

2. A liquid bacterial inoculation gun according to claim 1, characterized in that: The driving mechanism comprises a handle (13), a U-shaped bracket (14) fixedly connected to the upper end of the handle (13), and a U-shaped plate (15) rotatably connected to the U-shaped bracket (14); two wing rods of the U-shaped bracket (14) are rotatably connected to the two sides of the gun handle (10); two wing plates of the U-shaped plate (15) are rotatably connected to the two wing rods of the U-shaped bracket (14), respectively; a through hole having a diameter greater than that of the piston rod (9) is provided on the web of the U-shaped plate (15); a rear end portion of the piston rod (9) passes through the through hole provided with the U-shaped plate (15) and a thread is provided on the rear end portion; and a pressure plate is threadedly connected to the rear end portion of the piston rod (9).

3. The liquid bacterial inoculation gun according to claim 1, characterized in that: The inoculation needle (3) is threadedly connected to the second one-way valve (8).

4. The liquid bacterial inoculation gun according to claim 1, characterized in that: The one-way valve (7) is provided with a connecting pipe (16).

5. A liquid bacterial inoculation gun according to any one of claims 1 to 4, characterized in that: The joint (5) is threadedly connected to the gas cylinder (1).

6. The liquid bacterial inoculation gun according to claim 5, characterized in that: An O-shaped groove is provided on the connecting end surface of the gas cylinder (1) connected to the joint (5), and an O-shaped sealing ring (17) is provided in the O-shaped groove.

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