Stem cell cryopreservation device

Abstract

The stem cell cryopreservation device is characterized in that the stem cell cryopreservation device comprises a hexagonal-prism-shaped heat conduction block, and evenly-distributed insertion holes are formed in the positions, close to the prism faces, of the heat conduction block; a test tube filled with stem cells and culture substances to be frozen is inserted into the insertion hole; semiconductor chilling plates are attached to the side faces of the prism, a shell is further arranged at the bottom of the hexagonal prism, heat insulation plates are rotationally arranged on the shell and correspond to the side faces, a refrigerating fluid inlet pipe allowing refrigerating fluid to flow in is arranged at the top of the hexagonal prism, and a refrigerating fluid outlet pipe allowing the refrigerating fluid to flow out is arranged at the bottom of the hexagonal prism. According to the invention, slow refrigeration is realized by adopting a mode that the semiconductor refrigeration sheet which is relatively mild in refrigeration and the liquid nitrogen play a role in sections, so that stem cells are easy to preserve.

Description

Technical Field

[0001] The present application relates to the field of bioengineering and cell engineering equipment, and specifically to a stem cell cryopreservation device. Background Art

[0002] Cryopreservation of stem cells is a common process in cell engineering. Cultivated stem cells are generally frozen at ultra-low temperatures and thawed when needed to restore cell activity. Stem cells must be frozen slowly from the room temperature where they grow. If the temperature drops too quickly, such as using liquid nitrogen at minus 192 degrees Celsius for quick freezing, the water in the stem cells will freeze to form icicles that will pierce the cell membrane and the freezing will fail. Therefore, it is necessary to design a device that can slowly cryopreserve stem cells. Utility Model Content

[0003] 1. Technical issues

[0004] In view of the above-mentioned prior art, the present application proposes a device that can slowly perform stem cell cryopreservation.

[0005] (II) Technical solution

[0006] In response to the above technical problems, the present application proposes a stem cell cryopreservation device, which is characterized by: comprising a hexagonal heat-conducting block, on which evenly distributed sockets are arranged near the prism surface; test tubes containing stem cells and culture materials to be frozen are inserted into the sockets; semiconductor refrigeration plates are attached to the sides of the prism, and a shell is also arranged at the bottom of the hexagonal prism, on which heat insulation plates are rotatably arranged corresponding to each side surface, a refrigerant inlet pipe for refrigerant to flow in is arranged at the top of the hexagonal prism, and a refrigerant outlet pipe for refrigerant to flow out is arranged at the bottom of the hexagonal prism.

[0007] Furthermore, the hexagonal heat-conducting block is made of aluminum or copper.

[0008] Furthermore, the heat conductive block is provided with an adsorption strip which can adsorb the heat insulation board.

[0009] Furthermore, the refrigerant is liquid nitrogen.

[0010] (III) Beneficial effects

[0011] The present application adopts a semiconductor refrigeration sheet with relatively mild cooling and a liquid nitrogen segmented action method to achieve slow cooling and facilitate the preservation of stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is an overall schematic diagram according to the present application;

[0013] Figure 2 It is an overall schematic diagram of this application in another state;

[0014] Figure 3 It is a side view of the application in the first state;

[0015] Figure 4 for Figure 3 Top view of the .

[0016] Reference numerals

[0017] 1. Thermal block

[0018] 2. Test tube

[0019] 3. Semiconductor Refrigeration Chip

[0020] 4. Heat insulation board

[0021] 5. Refrigerant inlet pipe

[0022] 6. Housing

[0023] 7. Adsorption strip

[0024] 8. Refrigerant outlet pipe DETAILED DESCRIPTION

[0025] The utility model is further described below in conjunction with the embodiments.

[0026] like Figure 1 As shown, a stem cell cryopreservation device is characterized in that: it includes a hexagonal heat-conducting block 1, on which evenly distributed jacks are arranged near the prism surface; a test tube 2 containing stem cells and culture materials to be frozen is inserted into the jack; a semiconductor refrigeration sheet 3 is attached to the side of the prism, and a shell 6 is also arranged at the bottom of the hexagonal prism, and a heat insulation plate 4 is rotatably arranged on the shell corresponding to each side surface, a refrigerant inlet pipe 5 for refrigerant to flow in is arranged at the top of the hexagonal prism, and a refrigerant outlet pipe 8 for refrigerant to flow out is arranged at the bottom of the hexagonal prism.

[0027] In order to reduce the cooling speed of stem cells in the test tube, the present application designs a two-stage cooling method, first inserting the test tube into the socket, and then unfolding the insulation board to Figure 1 After the refrigeration process begins, the semiconductor refrigeration chip is powered on to cool it. The characteristic of the semiconductor refrigeration chip is that there is a certain temperature difference between its cold side and hot side. Therefore, in order to make the cold side have a cooling effect, the heat insulation board should be unfolded so that the outward side (hot side) of the semiconductor refrigeration chip can dissipate heat and the side that is attached to the heat conductive block can play a cooling role.

[0028] Furthermore, the hexagonal heat-conducting block is made of aluminum or copper.

[0029] The cooling capacity of the semiconductor refrigeration block is limited. Its cooling speed can meet the cooling requirements of stem cells, but after cooling to below zero, it is generally difficult to further cool. At this time, the refrigerant is introduced through the refrigerant inlet pipe and flows out from the refrigerant outlet pipe. When the refrigerant is used to further cool the test tube, the semiconductor refrigeration piece is in a power-off state. At this time, the heat insulation board is put away as shown in the figure. Figure 2 state.

[0030] Furthermore, the heat conductive block is provided with an adsorption strip which can adsorb the heat insulation board.

[0031] In this way, the present application has two states. The first is that when semiconductor refrigeration is used, the insulation board is unfolded, and the hot semiconductor refrigeration sheet can dissipate heat and cool; in the second state, when the temperature drops by a certain amount, the semiconductor refrigeration sheet is closed and the refrigerant starts to flow in and cool with the refrigerant. At this time, the insulation board is adsorbed onto the adsorption strip on the hexagonal prism and kept cold.

[0032] Furthermore, the refrigerant is liquid nitrogen.

[0033] If liquid nitrogen is used directly, because its temperature is minus 192 degrees, it is easy to cause the cooling speed to be too fast. The present application adopts a semiconductor refrigerator with milder cooling and a segmented liquid nitrogen operation method to achieve slow cooling and facilitate the preservation of stem cells.

[0034] The above is a detailed description of an embodiment of the utility model, but the content is only a preferred embodiment of the utility model and cannot be considered to limit the scope of implementation of the utility model. All equivalent changes and improvements made within the scope of application of the utility model should still fall within the scope of the patent coverage of the utility model.

Claims

1. A stem cell cryopreservation device, characterized in that: It comprises a hexagonal heat-conducting block, on which evenly distributed jacks are arranged near the prism faces; test tubes containing stem cells and culture materials to be frozen are inserted into the jacks; semiconductor cooling sheets are attached to the sides of the prism, and a shell is also arranged at the bottom of the hexagonal prism, on which heat insulation plates are rotatably arranged corresponding to the sides, a refrigerant inlet pipe for the refrigerant to flow in is arranged at the top of the hexagonal prism, and a refrigerant outlet pipe for the refrigerant to flow out is arranged at the bottom of the hexagonal prism.

2. A stem cell cryopreservation device according to claim 1, characterized in that: The hexagonal heat-conducting block is made of aluminum or copper.

3. A stem cell cryopreservation device according to claim 2, characterized in that: The heat conducting block is provided with an adsorption strip which can adsorb the heat insulation board.

4. A stem cell cryopreservation device according to claim 3, characterized in that: The refrigerant is liquid nitrogen.

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