Static mixing core for plasmid preparation

Abstract

The utility model belongs to the technical field of biology, and particularly relates to a static mixing core for plasmid preparation, which comprises a base plate, an overlapping plate and a top plate, mixing channels are arranged on the base plate, the overlapping plate and the top plate, a circulation block is arranged at the head quarter of each mixing channel, an inlet is arranged at one end of each mixing channel, a flow baffle is arranged at the inlet in each mixing channel, and the circulation block is arranged at the other end of each mixing channel. An outlet is formed in the tail end of the mixing channel, positioning holes are formed in the base plate, the stacking plate and the top plate, positioning columns are inserted into the positioning holes, L-shaped blocks are arranged on the base plate and the stacking plate, and clamping grooves are formed in the side faces of the stacking plate and the top plate. According to the utility model, a traditional stirring reactor is replaced by the mixing core, and two solutions are collided through a plurality of structures, so that the effect of fully mixing is achieved, the uniform mixing of different components can be stably and efficiently realized by the static mixing core in a plasmid production process, and a guarantee is provided for the preparation of high-quality plasmids.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a static mixing core used for plasmid preparation. Background Art

[0002] The static mixing core is a device used for plasmid preparation. It can mix different liquids or gases together by static mixing to achieve plasmid preparation.

[0003] During the plasmid preparation process, the bacterial culture containing the plasmid needs to be mixed with the lysis buffer to release the plasmid DNA. The static mixing core can fully mix the lysis buffer and the bacterial culture to improve the release efficiency and purity of the plasmid DNA.

[0004] In addition, the static mixing core can also be used in steps such as purification and concentration of plasmid DNA. By using it in conjunction with other equipment, automation and high-throughput plasmid preparation can be achieved.

[0005] In short, the static mixing core is an important plasmid preparation equipment, which can improve the efficiency and quality of plasmid preparation and provide strong support for the research and application of genetic engineering and biotechnology. Utility Model Content

[0006] 1. Technical issues to be solved

[0007] In view of the deficiencies of the prior art, the utility model provides a static mixing core for plasmid preparation, which solves the problems raised in the above background technology.

[0008] (II) Technical solution

[0009] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0010] A static mixing core for plasmid preparation comprises a base plate, a stacking plate and a top plate, wherein the base plate, the stacking plate and the top plate are all provided with a mixing channel, a circulation block is provided at the head of the mixing channel, an inlet is provided at one end of the mixing channel, a baffle is provided at the inlet in the mixing channel, an outlet is provided at the end of the mixing channel, positioning holes are provided on the base plate, the stacking plate and the top plate, positioning columns are plugged into the positioning holes, L-shaped blocks are provided on the base plate and the stacking plate, and card slots are provided on the sides of the stacking plate and the top plate.

[0011] Furthermore, the circulation block in the mixing channel divides the fluid into two left and right channels to form convection.

[0012] Furthermore, the L-shaped block is slidably engaged in the interior of the slot, the shape of the slot is adapted to the shape of the L-shaped block, and the opening length of the slot is greater than the length of the L-shaped block.

[0013] Furthermore, the designed length of the positioning column is consistent with the overall thickness of the base plate, the stacking plate and the top plate.

[0014] Furthermore, the L-shaped blocks are arranged at the four corners of the stacked plates and have the same orientation.

[0015] (III) Beneficial effects

[0016] Compared with the prior art, the utility model provides a static mixing core for plasmid preparation, which has the following beneficial effects:

[0017] The utility model replaces the traditional stirring reactor with a mixing core, and two solutions are fully mixed after a number of structural collisions, which meets the requirement of the plasmid production process. The static mixing core can stably and efficiently achieve uniform mixing of different components, thus providing a guarantee for high-quality plasmid preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the structure of the utility model;

[0019] Figure 2 It is the rear view of the structure of the utility model.

[0020] In the figure: 1, base plate; 2, stacking plate; 3, top plate; 4, mixing channel; 5, circulation block; 6, inlet; 7, baffle plate; 8, outlet; 9, positioning hole; 10, positioning column; 11, L-shaped block; 12, slot. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] Example

[0023] like Figure 1-2 As shown, a static mixing core for plasmid preparation proposed by an embodiment of the utility model comprises a base plate 1, a stacking plate 2 and a top plate 3, wherein the base plate 1, the stacking plate 2 and the top plate 3 are all provided with a mixing channel 4, a circulation block 5 is provided at the head of the mixing channel 4, an inlet 6 is provided at one end of the mixing channel 4, a baffle plate 7 is provided in the mixing channel 4, an outlet 8 is provided at the end of the mixing channel 4, positioning holes 9 are provided on the base plate 1, the stacking plate 2 and the top plate 3, a positioning column 10 is plugged into the positioning hole 9, an L-shaped block 11 is provided on the base plate 1 and the stacking plate 2, and a card slot 12 is provided on the side of the stacking plate 2 and the top plate 3;

[0024] Base plate 1, stacking plate 2 and top plate 3: they are the main components of the mixing core, which are stacked on each other and are provided with a clamping mold on the outside. The clamping mold is not drawn in the figure. The design of the stacking plate 2 can be multiple, and other structures such as the mixing channel 4 provide support and installation basis.

[0025] Mixing channel 4: distributed on each plate, with a circulation block 5 inside, an inlet 6 and a baffle 7 at one end, and an outlet 8 at the end, providing a channel for liquid flow, so that the fluid forms convection through the circulation block 5 to promote mixing; the inlet 6 is used for liquid entry, the baffle 7 can play a certain blocking and guiding role, and the outlet 8 is used for the mixed liquid to flow out.

[0026] Positioning holes 9 and positioning posts 10: They are provided on each board, and the positioning posts 10 are plugged into and matched with the positioning holes 9 to ensure that the base plate 1, the stacking plate 2 and the top plate 3 are accurately stacked and fixed, and to maintain the stability of the overall structure.

[0027] The L-shaped block 11 and the slot 12 enable the base plate 1 and the stacking plate 2, and the stacking plate 2 and the top plate 3 to slide relative to each other and be fixedly connected, which is convenient for installation and disassembly, while ensuring the integrity of the structure during operation.

[0028] like Figure 1 As shown, in some embodiments, the circulation block 5 in the mixing channel 4 divides the fluid into two left and right channels to form convection. The existence of the circulation block 5 enables the fluid entering the mixing channel 4 to be effectively divided into two left and right channels. This division promotes the formation of convection in a specific direction during the flow of the fluid, so that the fluids of different components can contact and merge with each other more fully and quickly, thereby improving the efficiency and uniformity of mixing.

[0029] like Figure 1 As shown, in some embodiments, the L-shaped block 11 is slidably engaged in the interior of the slot 12, the shape of the slot 12 is adapted to the shape of the L-shaped block 11, and the opening length of the slot 12 is greater than the length of the L-shaped block 11. During installation, the L-shaped block 11 can be easily slid into the slot 12, thereby reducing the difficulty of assembly. During the working process, if thermal expansion and contraction or other minor deformations occur, there can be a certain margin to adapt to ensure the integrity of the structure.

[0030] like Figure 2 As shown, in some embodiments, the design length of the positioning column 10 is consistent with the overall thickness of the substrate 1, the stacking plate 2 and the top plate 3, which can ensure that the substrate 1, the stacking plate 2 and the top plate 3 are accurately positioned when stacked without any upper and lower misalignment, thereby ensuring the precise alignment of structures such as the mixing channel 4, thereby maintaining a good mixing function.

[0031] like Figure 1As shown, in some embodiments, the L-shaped blocks 11 are arranged at the four corners of the stacked plate 2 and are oriented in the same direction. From the perspective of structural stability, the arrangement of the four corners can make the connection more stable, evenly share the force, and avoid local force concentration and structural instability. This layout can enhance the deformation resistance of the entire device during use. The consistent direction helps to ensure the consistency and standardization of the connection, making the cooperation between the layers more coordinated, and avoiding unsmooth connection or errors due to inconsistent directions of the L-shaped blocks 11.

[0032] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A static mixing core for plasmid preparation, comprising a base plate (1), a superimposed plate (2) and a top plate (3), characterized in that: The base plate (1), the stacked plate (2) and the top plate (3) are all provided with a mixing channel (4), a circulation block (5) is provided at the head of the mixing channel (4), an inlet (6) is provided at one end of the mixing channel (4), a baffle plate (7) is provided at the inlet (6) in the mixing channel (4), an outlet (8) is provided at the end of the mixing channel (4), positioning holes (9) are provided on the base plate (1), the stacked plate (2) and the top plate (3), positioning columns (10) are plugged into the positioning holes (9), L-shaped blocks (11) are provided on the base plate (1) and the stacked plate (2), and card slots (12) are provided on the sides of the stacked plate (2) and the top plate (3).

2. A static mixing core for plasmid preparation according to claim 1, characterized in that: The circulation block (5) in the mixing channel (4) divides the fluid into two left and right channels, thereby forming convection.

3. A static mixing core for plasmid preparation according to claim 1, characterized in that: The L-shaped block (11) is slidably engaged in the interior of the card slot (12); the shape of the card slot (12) is adapted to the shape of the L-shaped block (11); and the opening length of the card slot (12) is greater than the length of the L-shaped block (11).

4. A static mixing core for plasmid preparation according to claim 1, characterized in that: The designed length of the positioning column (10) is consistent with the overall thickness of the base plate (1), the stacking plate (2) and the top plate (3).

5. A static mixing core for plasmid preparation according to claim 1, characterized in that: The L-shaped blocks (11) are arranged at the four corners of the stacked plates (2) and are oriented in the same direction.

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