High performance liquid chromatograph mobile phase adding device

Abstract

The utility model discloses a mobile phase adding device of a high performance liquid chromatograph. The mobile phase adding device comprises a solvent bottle, and a connector and a mobile phase adding assembly are arranged on the solvent bottle; the connector is mounted at a bottle opening of the solvent bottle, and a connecting opening is formed in the connector; the mobile phase adding assembly comprises an organic phase adding assembly and a water phase adding assembly; the organic phase adding assembly and the water phase adding assembly are independently and alternately used, one ends of the organic phase adding assembly and the water phase adding assembly are detachably connected to the connecting port respectively, and the other ends are inserted into the solvent bottle and located below the liquid level of the mobile phase respectively. According to the liquid guide device provided by the invention, liquid is guided when the mobile phase is added, so that the mobile phase flows along the side wall of the liquid guide device, and the mobile phase adding assembly is inserted into the solvent bottle and is positioned below the liquid level of the mobile phase, so that bubbles are prevented from being generated when the liquid is added. The mobile phase adding assembly comprises an organic phase adding assembly and a water phase adding assembly, so that an organic phase and a water phase are independently added, and liquid phase detection operation does not need to be stopped.

Description

Technical Field

[0001] The present application relates to a mobile phase adding device for a high performance liquid chromatograph, and in particular to a mobile phase adding device for a high performance liquid chromatograph during a sample injection process, and belongs to the field of detection technology. Background Art

[0002] High performance liquid chromatography is a commonly used method for separating and measuring test samples in qualitative / quantitative analysis of compounds. It is a chromatographic method that uses a high-pressure infusion pump to pump the specified mobile phase into a chromatographic column filled with fillers to separate and measure the test samples. The injected test sample is carried into the chromatographic column by the mobile phase, and the components are separated in the column and enter the detector for detection. The chromatographic signal is recorded and processed by an integrator or data processing system. The equipment used is a high performance liquid chromatograph.

[0003] In the process of liquid chromatography, due to the limited capacity of the existing solvent bottle, when the sample inspection time is too long or there are too many samples to be inspected, the mobile phase in the mobile phase solvent bottle is insufficient and the mobile phase needs to be supplemented. When adding the mobile phase, the measurement process generally needs to be paused, and the pressure needs to be rebalanced after the mobile phase is added, and the balancing process takes too much time; therefore, the instrument is often not paused, and the mobile phase is added directly by slowly pouring from the mouth of the solvent bottle. However, direct pouring is prone to generate bubbles, which cause bubbles to enter the system, and the impact of the mobile phase causes the filter head position to change, which in turn causes pressure instability or peak position drift. Utility Model Content

[0004] In view of this, the present application provides a mobile phase adding device for a high performance liquid chromatograph, which is used to solve the problem in the prior art that bubbles or filter position errors are easily generated when the mobile phase is added directly without pausing the instrument, thereby causing unstable pressure or peak position drift. The specific scheme is:

[0005] A mobile phase adding device for a high performance liquid chromatograph comprises a solvent bottle body, wherein a connector and a mobile phase adding component are respectively arranged on the solvent bottle;

[0006] The connector is installed at the bottle mouth of the solvent bottle, and the connector is provided with a connecting port;

[0007] The mobile phase adding component comprises an organic phase adding component and an aqueous phase adding component;

[0008] The organic phase addition component and the aqueous phase addition component are used separately and alternately. One end of the organic phase addition component and the aqueous phase addition component are respectively detachably connected to the connection port, and the other ends of the organic phase addition component and the aqueous phase addition component are respectively inserted into the solvent bottle and are located below the liquid surface of the mobile phase.

[0009] Preferably, the organic phase addition component and the aqueous phase addition component respectively include a liquid guide, a connecting hose and a liquid infusion tube which are arranged in sequence from top to bottom;

[0010] The liquid guide is in the shape of a conical funnel, one end of the liquid guide is located above the bottle mouth of the solvent bottle, the other end of the liquid guide extends into the solvent bottle, and the liquid guide is detachably connected to the connection port of the connector;

[0011] One end of the connecting hose is connected to the liquid outlet end of the liquid guide device, and the other end of the connecting hose is connected to one end of the infusion tube;

[0012] One end of the infusion tube facing away from the connecting hose faces the bottom of the solvent bottle and is provided with a gap from the bottom of the bottle.

[0013] Preferably, the connector has an overall ring-shaped structure;

[0014] The cross section of the inner ring of the connector is a trapezoidal structure with a larger top and a smaller bottom, and the liquid guide is inserted into the inner ring of the connector;

[0015] The side of the connector in contact with the solvent bottle is provided with a slot matching the bottle mouth of the solvent bottle;

[0016] The card slot is buckled on the bottle mouth of the solvent bottle.

[0017] Preferably, the gap between one end of the infusion tube facing the bottom of the solvent bottle and the bottom of the bottle is less than 2 mm.

[0018] Preferably, the liquid guide is any one of a glass funnel or a plastic funnel.

[0019] Preferably, the connecting hose is a silicone tube.

[0020] Preferably, the infusion tube is a glass tube.

[0021] The beneficial effects of this application include:

[0022] The liquid guide provided in the present application is used to guide the liquid when the mobile phase is added, so that the mobile phase flows along the side wall of the liquid guide; the mobile phase addition component includes an organic phase addition component and an aqueous phase addition component, wherein one end of the organic phase addition component and the aqueous phase addition component are respectively detachably connected to the connection port of the liquid guide, and the other end is respectively inserted into the solvent bottle and located below the liquid surface of the mobile phase, while achieving the separate addition of the organic phase and the aqueous phase, the end of the mobile phase addition component is always kept below the liquid surface of the mobile phase to avoid the generation of bubbles when adding liquid, and at the same time, the mobile phase addition component is inserted to a position close to the bottom of the bottle to avoid the displacement of the filter head caused by the impact force when the mobile phase is added;

[0023] Moreover, when using the device, there is no need to stop the liquid phase detection operation, and the mobile phase can be replenished at any time, thereby avoiding the problem in the prior art that when adding the mobile phase, the pump needs to be stopped to add the mobile phase, and the pressure needs to be rebalanced after the pump is restarted, which results in excessive time consumption. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic cross-sectional structure diagram of a mobile phase adding device for a high performance liquid chromatography instrument of the present application;

[0025] Figure 2 This is a schematic cross-sectional structure diagram of a connector in a mobile phase adding device for a high performance liquid chromatograph of the present application;

[0026] Figure 3 This is an axonometric diagram of a mobile phase adding device for a high performance liquid chromatograph according to the present application.

[0027] List of parts and reference numerals:

[0028] 1. Solvent bottle; 2. Infusion tube; 3. Connecting hose; 4. Connector; 5. Liquid guide; 41. Card slot; 42. Slope surface. DETAILED DESCRIPTION

[0029] The present application is described in detail below with reference to embodiments, but the present application is not limited to these embodiments.

[0030] According to the attached Figure 1-3 A mobile phase adding device for a high performance liquid chromatograph is shown, comprising a solvent bottle 1 body, on which a connector 4 and a mobile phase adding component are respectively provided;

[0031] The connector 4 is installed at the bottle mouth of the solvent bottle 1, and the connector 4 is provided with a connecting port;

[0032] The mobile phase adding component comprises an organic phase adding component and an aqueous phase adding component;

[0033] The organic phase adding component and the aqueous phase adding component are used separately and alternately (that is, according to the type of mobile phase actually required to be added, the organic phase adding component or the aqueous phase adding component is selected to be used. For example, when it is necessary to add an organic phase, the organic phase adding component is installed at the connection port of the connector 4 to add the organic phase; when it is necessary to add an aqueous phase, the aqueous phase adding component is installed at the connection port of the connector 4 to add the aqueous phase), and the organic phase adding component and the aqueous phase adding component are detachably connected to the connector 4.

[0034] In the present application, the added phase component includes a separate organic phase addition component and an aqueous phase addition component, and the organic phase addition component and the aqueous phase addition component are used separately, which can reduce the detection error caused by solvent contamination and component ratio changes.

[0035] Furthermore, the organic phase adding component and the aqueous phase adding component respectively include a liquid guide 5, a connecting hose 3 and a liquid infusion tube 2 which are arranged in sequence from top to bottom;

[0036] One end of the liquid guide 5 is located above the bottle mouth of the solvent bottle 1, and the other end of the liquid guide 5 extends into the solvent bottle 1. The liquid guide 5 is detachably connected to the connection port of the connector 4;

[0037] One end of the connecting hose 3 is connected to the liquid outlet end of the liquid guide 5, and the other end of the connecting hose 3 is connected to one end of the infusion tube 2;

[0038] One end of the infusion tube 2 facing away from the connecting hose 3 faces the bottom of the solvent bottle 1 and is provided with a gap from the bottom of the bottle.

[0039] The liquid guide 5 is in the shape of a conical funnel, the liquid inlet end of the liquid guide 5 is arranged upward, the liquid outlet end of the liquid guide 5 extends into the solvent bottle 1, the liquid guide 5 is inserted into the connecting port of the connector 4, the liquid guide 5 matches the slope surface 42 of the inner ring of the connector 4, and the outer diameter of the liquid outlet end of the liquid guide 5 is smaller than the minimum diameter of the inner ring of the connector 4;

[0040] One end of the connecting hose 3 is sleeved on the outside of the liquid outlet end of the liquid guide device 5, and the other end of the connecting hose 3 is sleeved on the outside of the end of the infusion tube 2 facing the liquid guide device 5. The connecting hose 3 is used to connect the infusion tube 2 and the liquid guide device 5, and at the same time, the sealing performance of the connection is guaranteed.

[0041] Since the infusion tube 2 is a glass tube with a certain weight, the addition pipeline formed by the infusion tube 2 and the connecting hose 3 is always kept in a vertical state;

[0042] The end of the infusion tube 2 toward the liquid guide 5 and the outer side of the liquid outlet end of the liquid guide 5 (i.e., the neck of a funnel) both extend radially outward to a limiting portion to prevent the connecting hose 3 from being connected to the liquid outlet end of the liquid guide 5 and the outer side of the infusion tube 2;

[0043] One end of the infusion tube 2 facing the bottom of the solvent bottle 1 is provided with a gap from the bottom of the bottle, and the gap distance is less than 2 mm, so that the tube mouth of the infusion tube 2 can always be kept below the liquid surface of the mobile phase during the sample addition process, thereby avoiding bubbles.

[0044] When pouring, the added phase is poured closely against the side wall of the liquid-conducting body, and the added phase passes along the side wall of the liquid-conducting body in sequence.

[0045] Furthermore, the connector 4 has an overall ring-shaped structure;

[0046] The cross section of the inner ring of the connector 4 is a trapezoidal structure with a larger upper portion and a smaller lower portion, that is, the inner ring of the connector 4 forms a slope surface 42, and the liquid guide 5 is inserted into the inner ring of the connector 4;

[0047] In the present application, the liquid guide 5 is arranged in a funnel shape, and the inner ring of the connector 4 is a slope surface 42 matched with the outer wall of the liquid guide 5. When pressing downward, the connection between the liquid guide 5 and the connector 4 becomes tighter and tighter due to the influence of the slope surface 42, so as to avoid the shaking of the funnel when pouring the added phase; and when replacing the added phase component, it only needs to be taken out upward, which is convenient for replacing the added phase component;

[0048] The connector 4 is provided with a slot 41 on the side in contact with the solvent bottle 1, which matches the bottle mouth of the solvent bottle 1;

[0049] When the connector 4 is buckled onto the solvent bottle 1 , the bottle mouth of the solvent bottle 1 is inserted into the card slot 41 .

[0050] In one embodiment of the present application, the connector 4 is a silicone connector 4, which realizes connection and performs sealing function at the same time.

[0051] Furthermore, the liquid guide 5 is any one of a glass funnel and a plastic funnel.

[0052] In the present application, when the added phase is acid, alkaline water or other organic phase, the liquid guide 5 is a glass funnel, and when the added phase is water phase and organic phase, the liquid guide 5 is a plastic funnel.

[0053] Furthermore, the connecting hose 3 is a silicone tube.

[0054] Furthermore, the infusion tube 2 is a glass tube.

[0055] The use process of this device is:

[0056] Install the connector 4 at the opening of the solvent bottle 1, and make sure that the opening of the solvent bottle 1 is inserted into the card slot 41;

[0057] The two ends of the connecting hose 3 are respectively sleeved on the liquid outlet end of the liquid guide device 5 and one end of the infusion tube 2. Note that the tube head of the connecting hose 3 should be sleeved on the outer side of the liquid outlet end of the liquid guide device 5 and the limiting part of the infusion tube 2 to prevent the connecting hose 3 from falling off;

[0058] Insert the liquid guide 5 into the inner ring of the connector 4 and press it tightly by hand;

[0059] Finally, pour the mobile phase along the side wall of the liquid guide 5. The mobile phase enters the connecting hose 3, the infusion tube 2 and finally the mobile phase in the solvent bottle 1 along the side wall of the liquid guide 5 until the required amount is added. Note that during the addition process, when the mobile phase in the liquid phase bottle is slightly higher than or close to the filter head, the liquid phase pump and injection and other series of operations need to be stopped, that is, the ongoing detection work should be stopped. After the addition is completed, the liquid guide 5, the connecting hose 3 and the infusion tube 2 should be cleaned in time.

[0060] The above are only a few embodiments of the present application and do not constitute any form of limitation to the present application. Although the present application is disclosed as above with preferred embodiments, it is not intended to limit the present application. Any technician familiar with the profession, without departing from the scope of the technical solution of the present application, using the technical content disclosed above to make slight changes or modifications are equivalent to equivalent implementation cases and fall within the scope of the technical solution.

Claims

1. A mobile phase adding device for a high performance liquid chromatograph, comprising a solvent bottle body, characterized in that: The solvent bottle is respectively provided with a connector and a mobile phase adding component; The connector is installed at the bottle mouth of the solvent bottle, and the connector is provided with a connecting port; The mobile phase adding component comprises an organic phase adding component and an aqueous phase adding component; The organic phase addition component and the aqueous phase addition component are used separately and alternately. One end of the organic phase addition component and the aqueous phase addition component are respectively detachably connected to the connection port, and the other end of the organic phase addition component and the aqueous phase addition component are respectively inserted into the solvent bottle and located below the liquid surface of the mobile phase.

2. A mobile phase adding device for high performance liquid chromatography according to claim 1, characterized in that: The organic phase adding component and the aqueous phase adding component respectively include a liquid guide, a connecting hose and a liquid infusion tube which are arranged in sequence from top to bottom; The liquid guide is in the shape of a conical funnel, one end of the liquid guide is located above the bottle mouth of the solvent bottle, the other end of the liquid guide extends into the solvent bottle, and the liquid guide is detachably connected to the connection port of the connector; One end of the connecting hose is connected to the liquid outlet end of the liquid guide device, and the other end of the connecting hose is connected to one end of the infusion tube; One end of the infusion tube facing away from the connecting hose faces the bottom of the solvent bottle and is provided with a gap from the bottom of the bottle.

3. A mobile phase adding device for high performance liquid chromatography according to claim 2, characterized in that: The connector is in an annular structure as a whole; The cross section of the inner ring of the connector is a trapezoidal structure with a larger top and a smaller bottom, and the liquid guide is inserted into the inner ring of the connector; The side of the connector in contact with the solvent bottle is provided with a slot matching the bottle mouth of the solvent bottle; The card slot is buckled on the bottle mouth of the solvent bottle.

4. A mobile phase adding device for high performance liquid chromatography according to claim 2, characterized in that, The gap between one end of the infusion tube facing the bottom of the solvent bottle and the bottom of the bottle is less than 2 mm.

5. A mobile phase adding device for high performance liquid chromatography according to claim 2, characterized in that: The liquid guide is any one of a glass funnel and a plastic funnel.

6. A mobile phase adding device for high performance liquid chromatography according to claim 2, characterized in that: The connecting hose is a silicone tube.

7. A mobile phase adding device for high performance liquid chromatography according to claim 2, characterized in that: The infusion tube is a glass tube.

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