Pet stem cell multiplication culture and extraction integrated equipment

Abstract

The utility model discloses pet stem cell multiplication culture and extraction integrated equipment, which is applied to the field of medical equipment.The pet stem cell multiplication culture and extraction integrated equipment is characterized in that when a rotating cylinder is rotated, a connecting rod drives a clamping rod to move, the clamping rod moves to enable the clamping rod to be disengaged from a clamping groove, then a heating pipe is taken out, and a new heating pipe is inserted into a box body; and the clamping rods are clamped with the clamping grooves under the action of the first springs to fix the clamping grooves and complete replacement, so that the purpose of conveniently replacing the heating pipes is achieved, the replacement process is simple, the workload is reduced, and the replacement efficiency is improved. By rotating the rotating disc, the first bevel gear drives the second bevel gear to rotate, the second bevel gear rotates to drive the transmission rod to rotate, the transmission rod rotates to drive the fixing rod to move, the fixing rod moves to be disengaged from the fixing groove, and then the cover plate is opened for maintenance, the purpose of facilitating maintenance can be achieved. The workload is reduced, and the maintenance efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the field of medical equipment, in particular to an integrated device for pet stem cell expansion, cultivation and extraction. Background Art

[0002] Hematopoietic stem cells are adult stem cells in the blood system. They are a heterogeneous group with long-term self-renewal capabilities and the potential to differentiate into various mature blood cells. They are the type of adult stem cells with the longest and most in-depth research history. They have important guiding significance for the study of various stem cells, including tumor stem cells. When conducting experimental research, it is necessary to first expand and culture the hematopoietic stem cells, and then extract the cells.

[0003] The existing integrated expansion, culture and extraction equipment is not convenient for replacing the heating tube. If the heating tube is damaged during use, it needs to be replaced. The heating tube is generally installed inside the box. When replacing it, the equipment needs to be disassembled, and then the heating tube is disassembled and replaced. After the replacement is completed, the equipment is installed again. The replacement process is relatively cumbersome, which increases the workload and reduces the efficiency of replacement. It is also not convenient for maintenance. The equipment needs to be maintained inside after use to ensure the normal operation of the equipment. The cover plate and the inner tank of the equipment are fixed and installed with multiple bolts. During maintenance, tools are needed to disassemble and assemble it. The disassembly and assembly process is relatively cumbersome, which increases the workload and reduces the maintenance efficiency. In order to solve the above-mentioned problems, we propose an integrated pet stem cell expansion, culture and extraction equipment. Utility Model Content

[0004] The utility model aims to provide an integrated device for pet stem cell expansion, culture and extraction, which has the advantages of being easy to replace a heating tube and easy to maintain.

[0005] The above technical objectives of the utility model are achieved through the following technical solutions: an integrated device for pet stem cell expansion, culture and extraction, comprising a box body, the bottom of which is bolted with supporting legs, an inner liner arranged radially spaced from the inner wall of the box body is provided in the box body, and a heat transfer fluid is injected between the inner liner and the inner wall of the box body; a feeding pipe connected to the interior of the inner liner is provided on the box body, and a stirring mechanism is provided inside the inner liner; a fixed cylinder is bolted to the surface of the box body, a heating tube extending into the heat transfer fluid is slidably sleeved inside the fixed cylinder, and a rotating cylinder is rotatably sleeved outside the fixed cylinder, and a connecting rod is hinged on the inner wall of the rotating cylinder; a wedge-shaped hole for the connecting rod to swing is provided in the fixed cylinder, and a clamping rod that extends and contracts radially is installed in the fixed cylinder, and the outer end of the clamping rod extends into the wedge-shaped hole and is connected to the connecting rod The hinge is connected, and a limit plate is fixedly sleeved on the surface of the clamping rod to prevent the clamping rod from escaping from the fixing tube, and a first spring is arranged between the limit plate and the hole wall for installing the clamping rod; a clamping groove corresponding to the clamping rod is provided on the surface of the heating tube, and the clamping groove is plugged and matched with the inner end of the clamping rod; a cover plate closed to it is provided on the top of the inner tank, and a rotatable rotating shaft is installed at the center of the cover plate, and a first bevel gear is fixedly sleeved on the lower end of the rotating shaft, and a second bevel gear is meshed on the surface of the first bevel gear, and a transmission rod extending radially along the cover plate is fixed at the axis of the second bevel gear, and the transmission rod is rotatably matched with the cover plate; a threaded fixing rod is plugged at the extending end of the transmission rod, and the fixing rod can slide but not rotate relative to the cover plate, and a fixing groove is provided on the inner wall of the inner tank, and the fixing groove is plugged and matched with the fixing rod.

[0006] By adopting the above technical solution, by rotating the rotating cylinder, the rotating cylinder rotates to drive the connecting rod to swing, the connecting rod pulls the clamping rod backward, the first spring is compressed, the clamping rod withdraws from the clamping groove, and then the heating tube is taken out and a new heating tube is inserted into the box body; when the clamping groove on the heating tube corresponds to the inner hole on the fixed cylinder for the clamping rod to be installed, the clamping rod is inserted into the clamping groove under the action of the first spring, the fixed cylinder and the heating tube are fixed, and the replacement is completed, thereby achieving the purpose of facilitating the replacement of the heating tube, the replacement process is simple, the workload is reduced, and the replacement efficiency is improved.

[0007] By rotating the shaft, the rotation of the shaft drives the first bevel gear to rotate, the rotation of the first bevel gear drives the second bevel gear to rotate, the rotation of the second bevel gear drives the transmission rod to rotate, the rotation of the transmission rod drives the fixed rod to move, so that the fixed rod exits the fixed groove, and finally the cover is opened for maintenance, which can achieve the purpose of facilitating maintenance, reduce workload, and improve maintenance efficiency.

[0008] The utility model is further configured as follows: the stirring mechanism comprises a motor, the motor is bolted to the bottom of the box body, the output end of the motor extends to the inside of the inner tank and is connected to a rotating rod through a coupling, and the surface of the rotating rod is bolted to a stirring rod.

[0009] By adopting the above technical solution and setting a stirring mechanism, the culture efficiency is improved and the use is convenient.

[0010] The utility model is further configured as follows: a sealing ring is embedded in the upper end surface of the inner container, and a second spring supporting the sealing ring is installed in the groove for embedding the sealing ring.

[0011] By adopting the above technical solution and providing a sealing ring and a second spring, the sealing performance is improved.

[0012] The utility model is further configured as follows: a separator communicated with the interior of the inner container is arranged on the box body, and a valve is installed at the outlet of the separator.

[0013] By adopting the above technical solution and providing a separator and a valve, it is easy to separate and extract stem cells, thereby improving practicality.

[0014] The utility model is further configured as follows: a pad is bolted to the bottom of the support leg.

[0015] By adopting the above technical solution and providing a pad, the stability of the device is improved.

[0016] The utility model is further configured as follows: the surface of the rotating cylinder is provided with anti-slip grooves.

[0017] By adopting the above technical solution, anti-skid patterns are provided to prevent hands from slipping and facilitate operation.

[0018] The utility model is further configured as follows: a rotating disk is bolted to the top of the rotating shaft.

[0019] By adopting the above technical solution, the rotating shaft can be easily rotated by arranging a rotating disk.

[0020] In summary, the utility model has the following beneficial effects:

[0021] 1. The utility model rotates the rotating cylinder, and the rotation of the rotating cylinder drives the connecting rod to swing, and the connecting rod pulls the clamping rod backward, the first spring is compressed, the clamping rod exits the clamping groove, and then the heating tube is taken out, and a new heating tube is inserted into the box body; when the clamping groove on the heating tube corresponds to the inner hole on the fixed cylinder for the clamping rod to be installed, the clamping rod is inserted into the clamping groove under the action of the first spring, and the fixed cylinder and the heating tube are fixed to complete the replacement, thereby achieving the purpose of facilitating the replacement of the heating tube, simplifying the replacement process, reducing the workload, and improving the replacement efficiency.

[0022] 2. By rotating the shaft, the rotation of the shaft drives the first bevel gear to rotate, the rotation of the first bevel gear drives the second bevel gear to rotate, the rotation of the second bevel gear drives the transmission rod to rotate, the rotation of the transmission rod drives the fixed rod to move, so that the fixed rod exits the fixed groove, and finally the cover is opened for maintenance, which can achieve the purpose of facilitating maintenance, reduce workload, and improve maintenance efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a three-dimensional diagram of the structure of the utility model;

[0024] Figure 2 It is a cross-sectional view of the structure of the utility model;

[0025] Figure 3 It is a top view cross-sectional view of the local structure of the utility model;

[0026] Figure 4 It is a top view cross-sectional view of the local structure of the utility model;

[0027] Figure 5 This utility model Figure 2 A magnified view of the structure at center;

[0028] Figure 6 This utility model Figure 2 Enlarged view of the structure at point B in the middle.

[0029] Figure numerals: 1. box body; 2. supporting legs; 3. liner; 4. heat transfer liquid; 5. feeding pipe; 6. stirring mechanism; 7. fixed cylinder; 8. heating tube; 9. rotating cylinder; 10. connecting rod; 11. clamping rod; 12. limit plate; 13. first spring; 14. clamping groove; 15. cover plate; 16. rotating shaft; 17. first bevel gear; 18. second bevel gear; 19. transmission rod; 20. fixed rod; 21. fixed groove; 22. motor; 23. rotating rod; 24. stirring rod; 25. sealing ring; 26. second spring; 27. separator; 28. valve; 29. ​​pad; 30. anti-slip pattern; 31. rotating disk. DETAILED DESCRIPTION

[0030] The utility model is further described in detail below in conjunction with the accompanying drawings.

[0031] Embodiment 1:

[0032] refer to Figure 1 , Figure 2 , Figure 3 and Figure 5, a pet stem cell expansion, culture and extraction integrated equipment, including a box body 1, a support leg 2 is bolted to the bottom of the box body 1, an inner liner 3 is arranged radially spaced from the inner wall of the box body 1, and a heat transfer fluid 4 is injected between the inner liner 3 and the inner wall of the box body 1; a feeding pipe 5 connected to the inner liner 3 is provided on the box body 1, and a stirring mechanism 6 is provided inside the inner liner 3. A fixed cylinder 7 is bolted to the surface of the box body 1, and a heating tube 8 extending into the heat transfer fluid 4 is slidably sleeved inside the fixed cylinder 7, and a rotating cylinder 9 is rotatably sleeved outside the fixed cylinder 7, and the inner wall of the rotating cylinder 9 is hinged There is a connecting rod 10, and a wedge-shaped hole is provided in the fixed cylinder 7 for the connecting rod 10 to swing; a clamping rod 11 which is retractable along its radial direction is installed in the fixed cylinder 7, and the outer end of the clamping rod 11 extends into the wedge-shaped hole and is hinged with the connecting rod 10, and a limiting plate 12 is fixedly sleeved on the surface of the clamping rod 11 to prevent the clamping rod 11 from escaping from the fixed cylinder 7, and a first spring 13 is provided between the limiting plate 12 and the hole wall for installing the clamping rod 11; a clamping groove 14 corresponding to the clamping rod 11 is provided on the surface of the heating tube 8, and the clamping groove 14 is plugged into and matched with the inner end of the clamping rod 11. By rotating the rotating cylinder 9, the rotating cylinder 9 rotates to drive the connecting rod 10 to swing, and the connecting rod 10 pulls the clamping rod 11 backward, the first spring 13 is compressed, and the clamping rod 11 withdraws from the clamping groove 14, then the heating tube 8 is taken out, and the new heating tube 8 is inserted into the box body 1. When the clamping groove 14 on the heating tube 8 corresponds to the inner hole on the fixed cylinder 7 for installing the clamping rod 1, the clamping rod 11 is inserted into the clamping groove 14 under the action of the first spring 13, and the fixed cylinder 7 and the heating tube 8 are fixed to complete the replacement, thereby achieving the purpose of facilitating the replacement of the heating tube 8, the replacement process is simple, the workload is reduced, and the replacement efficiency is improved.

[0033] refer to Figure 2 The stirring mechanism 6 includes a motor 22, which is bolted to the bottom of the box body 1. The output end of the motor 22 extends to the inside of the inner tank 3 and is connected to a rotating rod 23 through a coupling. The surface of the rotating rod 23 is bolted to a stirring rod 24. By setting the stirring mechanism 6, the efficiency of the culture is improved and the use is convenient.

[0034] refer to Figure 1 and Figure 2 The box body 1 is provided with a separator 27 which is in communication with the interior of the liner 3, and a valve 28 is installed at the outlet of the separator 27. By providing the separator 27 and the valve 28, it is convenient to separate and extract stem cells, and the practicality is improved.

[0035] refer to Figure 1 and Figure 2 A pad 29 is bolted to the bottom of the support leg 2. By providing the pad 29, the stability of the device is improved.

[0036] refer to Figure 3 The surface of the rotating cylinder 9 is provided with anti-skid lines 30. By arranging the anti-skid lines 30, hand slip is prevented and operation is facilitated.

[0037] Embodiment 2:

[0038] refer to Figure 1 , Figure 2 , Figure 4 and Figure 5 A cover plate 15 is provided on the top of the inner liner 3 to close it, and a rotatable shaft 16 is installed at the center of the cover plate 15. A first bevel gear 17 is fixedly sleeved on the lower end of the shaft 16. A second bevel gear 18 is meshed on the surface of the first bevel gear 17. A transmission rod 19 extending radially along the cover plate 15 is fixed at the axis of the second bevel gear 18. The transmission rod 19 is rotatably matched with the cover plate 15. A threaded fixing rod 20 is inserted at the extended end of the transmission rod 19. The fixing rod 20 can slide relative to the cover plate 15 but cannot rotate, and a fixing groove 21 is opened on the inner wall of the inner liner 3, and the fixing groove 21 is plugged into the fixing rod 20.

[0039] By rotating the rotating shaft 16, the rotating shaft 16 drives the first bevel gear 17 to rotate, the first bevel gear 17 drives the second bevel gear 18 to rotate, the second bevel gear 18 drives the transmission rod 19 to rotate, the transmission rod 19 drives the fixing rod 20 to move, so that the fixing rod 20 exits the fixing groove 21, and finally the cover plate 15 is opened for maintenance, which can achieve the purpose of facilitating maintenance, reduce workload, and improve maintenance efficiency.

[0040] refer to Figure 6 A sealing ring 25 is embedded in the upper end surface of the inner liner 3, and a second spring 26 supporting the sealing ring 25 is installed in the groove for embedding the sealing ring 25. By arranging the sealing ring 25 and the second spring 26, the sealing performance is improved.

[0041] refer to Figure 1 A rotating disk 31 is bolted to the top of the rotating shaft 16 , and the rotating disk 31 is provided to facilitate the rotation of the rotating shaft 16 .

[0042] The use process is briefly described as follows: the rotating cylinder 9 is rotated, and the rotating cylinder 9 rotates to drive the connecting rod 10 to move, and the connecting rod 10 moves to drive the clamping rod 11 to move, and the clamping rod 11 moves to disengage the clamping rod 11 from the clamping groove 14, and then the heating tube 8 is taken out, and a new heating tube 8 is inserted into the box body 1, and the rotating cylinder 9 is reset. The clamping rod 11 is clamped with the clamping groove 14 under the action of the first spring 13, and it is fixed to complete the replacement, thereby achieving the purpose of facilitating the replacement of the heating tube 8; the rotating disk 31 is rotated, and the rotating disk 31 rotates to drive the first bevel gear 17 to rotate, and the first bevel gear 17 rotates to drive the second bevel gear 18 to rotate, and the second bevel gear 18 rotates to drive the transmission rod 19 to rotate, and the transmission rod 19 rotates to drive the fixing rod 20 to move, and the fixing rod 20 exits the fixing groove 21, and then the cover plate 15 is opened for maintenance. After maintenance, the cover plate 15 is covered, and the rotating disk 31 is rotated in the opposite direction to make the fixing rod 20 clamped with the fixing groove 21, and the cover plate 15 is fixed to complete the maintenance, thereby achieving the purpose of facilitating maintenance.

[0043] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but such modifications are protected by patent law as long as they are within the scope of the claims of the present invention.

Claims

1. An integrated device for pet stem cell expansion, culture and extraction, comprising a housing (1), characterized in that: The bottom of the box (1) is bolted with a support leg (2), an inner liner (3) is arranged radially spaced from the inner wall of the box (1), and a heat transfer fluid (4) is injected between the inner liner (3) and the inner wall of the box (1); a feed pipe (5) connected to the inner liner (3) is provided on the box, and a stirring mechanism (6) is provided inside the inner liner (3); a fixed cylinder (7) is bolted to the surface of the box (1), a heating tube (8) extending into the heat transfer fluid (4) is slidably sleeved inside the fixed cylinder (7), and a rotating cylinder (9) is rotatably sleeved outside the fixed cylinder (7), and a connecting rod (10) is hinged on the inner wall of the rotating cylinder (9); The fixing tube (7) is provided with a wedge-shaped hole for the connecting rod (10) to swing, and a clamping rod (11) is installed in the fixing tube (7) to extend and retract along its radial direction, the outer end of the clamping rod (11) extends into the wedge-shaped hole and is hinged with the connecting rod (10), and a limiting plate (12) is fixedly sleeved on the surface of the clamping rod (11) to prevent the clamping rod (11) from escaping from the fixing tube, and a first spring (13) is arranged between the limiting plate (12) and the hole wall where the clamping rod (11) is installed; the surface of the heating tube (8) is provided with a clamping groove (14) corresponding to the clamping rod (11), and the clamping groove (14) is plugged into and matched with the inner end of the clamping rod (11).

2. The pet stem cell expansion, culture and extraction integrated device according to claim 1, characterized in that: The top of the inner container (3) is provided with a cover plate (15) which is closed therefrom. A rotatable shaft (16) is installed at the center of the cover plate (15). A first bevel gear (17) is fixedly sleeved on the lower end of the shaft (16). A second bevel gear (18) is meshed on the surface of the first bevel gear (17). A transmission rod (19) extending radially along the cover plate (15) is fixed at the axis of the second bevel gear (18). The transmission rod (19) is rotatably matched with the cover plate (15). A threaded fixing rod (20) is inserted at the extended end of the transmission rod (19). The fixing rod (20) is slidable but not rotatable relative to the cover plate (15). A fixing groove (21) is provided on the inner wall of the inner container (3). The fixing groove (21) is plugged and matched with the fixing rod (20).

3. The integrated device for pet stem cell expansion, culture and extraction according to claim 1, characterized in that: The stirring mechanism (6) comprises a motor (22), the motor (22) is bolted to the bottom of the box (1), the output end of the motor (22) extends to the interior of the inner pot (3) and is connected to a rotating rod (23) via a coupling, and a stirring rod (24) is bolted to the surface of the rotating rod (23).

4. The pet stem cell expansion, culture and extraction integrated device according to claim 1, characterized in that: A sealing ring (25) is embedded in the upper end surface of the inner container (3), and a second spring (26) supporting the sealing ring (25) is installed in the groove for embedding the sealing ring (25).

5. The pet stem cell expansion, culture and extraction integrated device according to claim 1, characterized in that: The box body is provided with a separator (27) which is in communication with the interior of the inner container (3), and a valve (28) is installed at the outlet of the separator (27).

6. The pet stem cell expansion, culture and extraction integrated device according to claim 1, characterized in that: A pad (29) is bolted to the bottom of the support leg (2).

7. The integrated device for pet stem cell expansion, culture and extraction according to claim 1, characterized in that: The surface of the rotating cylinder (9) is provided with anti-slip grooves (30).

8. The integrated device for pet stem cell expansion, culture and extraction according to claim 2, characterized in that: A rotating disk (31) is bolted to the top of the rotating shaft (16).

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