Micro-fluidic chip sample loading device

Abstract

The utility model relates to the technical field of microfluidics, and discloses a microfluidic chip sample loading device which comprises a base, a sample loading platform, a chip, a gland and a limiting assembly, a rotating seat is arranged on the base; the sample loading platform is mounted on the base, and a plurality of reagent grooves are formed in the sample loading platform; the chip is arranged on one side, away from the reagent groove, of the sample loading platform, and a micro-channel capable of being communicated with the reagent groove is arranged in the chip; the gland is rotationally connected to the rotating seat, and the gland is provided with a press-fit position abutting against the base in a sealed mode and an opening position opened relative to the base; the limiting assembly comprises an elastic pushing piece and a limiting groove, one of the elastic pushing piece and the limiting groove is arranged on the gland, and the other one of the elastic pushing piece and the limiting groove is arranged on the rotating seat; when the gland rotates to the opening position, the elastic pushing piece can be inserted into and clamped in the limiting groove so as to limit rotation of the gland. After the gland is opened, the position of the gland can be automatically locked, and the situation that the gland is automatically pressed down due to the gravity effect, and the sample loading process is affected is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidics, in particular to a microfluidics chip sample loading device. Background Art

[0002] Microfluidics is a technology that uses microchannels (with a size of tens to hundreds of microns) to process or manipulate tiny fluids (with a volume of nanoliters to attoliters). Microfluidics has the advantages of light volume, small sample size, low energy consumption, fast response, and large-scale parallel processing. It is widely used in in vitro diagnosis, organ chips, cell manipulation, etc. The core of microfluidics technology is the microfluidic chip. Different microfluidic chip flow channel structures are designed according to different application requirements to realize the processes of microfluidic injection, operation, reaction, and sample output.

[0003] Existing microfluidic loading devices usually include a base, a loading platform disposed on the base, and a pressure cover rotatably disposed on the base. However, when the pressure cover is opened and used, it is often automatically pressed down due to gravity, thereby affecting the normal loading process of the microfluidic chip.

[0004] Therefore, there is an urgent need for a microfluidic chip sample loading device to solve the above technical problems. Utility Model Content

[0005] Based on the above, the purpose of the utility model is to provide a microfluidic chip loading device, which can automatically lock the position of the pressure cover when it is rotated to the open position to prevent the pressure cover from automatically pressing down due to gravity and affecting the loading process.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] Microfluidic chip loading device, comprising:

[0008] A base, wherein a rotating base is provided on the base;

[0009] A sample loading platform is installed on the base, and a plurality of reagent tanks are arranged on the sample loading platform;

[0010] A chip is arranged on a side of the sample loading platform away from the reagent tank, and a microchannel that can communicate with the reagent tank is arranged in the chip;

[0011] A gland, rotatably connected to the rotating seat, the gland having a pressed position in sealing contact with the base and an open position open relative to the base;

[0012] The limiting assembly includes an elastic pushing member and a limiting groove, wherein one of the elastic pushing member and the limiting groove is arranged on the pressure cover, and the other is arranged on the rotating seat; when the pressure cover is rotated to the open position, the elastic pushing member can be inserted into and clamped in the limiting groove to limit the rotation of the pressure cover.

[0013] In some possible implementations, the loading platform is further provided with a plurality of gas source control ports for controlling the connection or blocking of the flow path in the microchannel by means of pressurized gas.

[0014] In some possible embodiments, the reagent tank includes a sample loading tank, a waste liquid tank and a collection tank, the sample loading tank is connected to the inlet of the microfluidic channel, the waste liquid tank is connected to the waste liquid outlet of the microfluidic channel, and the collection tank is connected to the collection liquid outlet of the microfluidic channel; the reagent in the sample loading tank can enter the microfluidic channel under the drive of the pressurized gas, and finally enter the waste liquid tank or the collection tank.

[0015] In some possible embodiments, an air inlet is provided on the inner side of the pressure cover and is located opposite to the sample loading slot and the air source control port. An air flow channel is also provided inside the pressure cover, and the outlet end of the air flow channel is connected to the air inlet, and the inlet end of the air flow channel is sealed and connected to an air pipe, and the air pipe is used to communicate with an external air source; the pressure cover is also provided with an air outlet relative to the waste liquid tank and the collection tank.

[0016] In some possible implementations, the air pipe is sealedly connected to the inlet end of the air flow channel through a UNF threaded joint.

[0017] In some possible implementations, sealing members are provided between the reagent tank and the pressure cover, and between the gas source control port and the pressure cover; when the pressure cover is rotated to the pressing position, the pressure cover is sealed and connected to the reagent tank and the gas source control port.

[0018] In some possible embodiments, a plurality of sealing grooves are provided on the pressure cover, and the air inlet and the air outlet are respectively provided in the corresponding sealing grooves; the upper ends of the reagent tank and the gas source control port are both protruded on the loading platform for plugging with the corresponding sealing grooves; the sealing member is a sealing ring, and the sealing ring is fixed in the sealing groove.

[0019] In some possible implementations, the loading platform and the chip are both made of transparent materials, and the gland is provided with an observation port for observing the reagent status in the chip; and / or,

[0020] The elastic pushing member is a spring pin, which is installed on the inner side of the rotating seat. The limiting groove is arranged on the pressure cover and close to the rear end of the pressure cover.

[0021] In some possible embodiments, the microfluidic chip loading device also includes an adapter plate, which is arranged between the loading platform and the chip, and is provided with a plurality of first through holes and a plurality of second through holes, wherein the first through holes are used to connect the reagent tank with the inlet or outlet of the microchannel, and the second through holes are used to connect the gas source control port with the membrane valve interface of the microchannel.

[0022] In some possible embodiments, the microfluidic chip loading device also includes a quick-locking component, which includes a first locking member and a second locking member, one of the first locking member and the second locking member is arranged on the pressure cover, and the other is arranged on the base; when the pressure cover is rotated to the pressing position, the first locking member can be locked with the second locking member to make the pressure cover seal and abut against the upper end of the reagent tank.

[0023] The beneficial effects of the utility model are:

[0024] The microfluidic chip loading device provided by the utility model has a pressure cover that is rotatably connected to a rotating seat. When the pressure cover is rotated to a pressing position, the pressure cover can be sealed against the base, so that the reagent can flow between the reagent tank and the chip microchannel in a sealed environment, avoiding gas or liquid leakage that affects the use of the microfluidic chip; a limiting component is also provided between the pressure cover and the rotating seat, and when the pressure cover is rotated to an open position, an elastic push piece can be automatically inserted and snapped into the limiting groove, thereby limiting the continued rotation of the pressure cover, which is simple to operate and reliable to lock, effectively avoiding the pressure cover from automatically pressing down due to gravity and affecting the loading process. The microfluidic chip loading device has a compact structure and is easy to use. The operator only needs to add the reagent to the reagent tank and cover the pressure cover, and the reagent can be driven to flow in the reagent tank and the chip through a power source to realize the loading process. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the structure of the microfluidic chip sample loading device provided by the embodiment of the utility model;

[0026] Figure 2 It is a schematic diagram of the structure of the gland and the air pipe provided in the embodiment of the utility model;

[0027] Figure 3 It is a schematic diagram of the structure of the gland provided by the embodiment of the utility model;

[0028] Figure 4 This is an exploded view of the sample loading platform, adapter plate and chip provided in the embodiment of the utility model;

[0029] Figure 5 It is a structural schematic diagram of a rotating seat provided in an embodiment of the utility model;

[0030] Figure 6 It is a structural schematic diagram of a first locking member provided in an embodiment of the utility model;

[0031] Figure 7 It is a structural schematic diagram of a second locking member provided by an embodiment of the utility model from one perspective;

[0032] Figure 8 It is a structural schematic diagram of the second locking member provided by an embodiment of the utility model from another perspective.

[0033] In the figure:

[0034] 1. Base; 11. Rotating seat; 111. Support block; 112. Axial hole; 2. Sample loading platform; 21. Reagent tank; 211. Sample loading tank; 212. Waste liquid tank; 213. Collecting tank; 22. Gas source control port; 3. Chip; 4. Pressure cover; 41. Air inlet; 42. Air outlet; 43. Observation port; 51. Elastic push member; 52. Limiting groove; 6. Air pipe; 61. UNF threaded connector; 7. Adapter plate; 71. First through hole; 72. Second through hole; 81. First locking member; 811. Rotating part; 812. Locking part; 82. Second locking member; 821. First locking cavity; 822. Second locking cavity. DETAILED DESCRIPTION

[0035] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It should also be noted that, for ease of description, only the parts related to the present invention, rather than all structures, are shown in the accompanying drawings.

[0036] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0037] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0038] In the description of this embodiment, the terms "upper", "lower", "left", "right", etc., and the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of description and simplified operation, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first" and "second" are only used to distinguish in the description and have no special meaning.

[0039] like Figure 1-Figure 8 As shown, the present embodiment provides a microfluidic chip loading device, including a base 1, a loading platform 2, a chip 3, a pressure cover 4 and a limit assembly. The base 1 is the bearing structure of the entire device, and is provided with a mounting groove, and the loading platform 2 is installed in the mounting groove. A plurality of reagent tanks 21 are provided on the loading platform 2 for containing different types of reagents. The chip 3 is arranged on the side of the loading platform 2 away from the reagent tank 21, and a microchannel that can be connected to the reagent tank 21 is provided in the chip 3. Specifically, the chip 3 of the present embodiment can be fixedly connected to the loading platform 2 by bonding. A rotating seat 11 is also fixed on the base 1, and the pressure cover 4 is rotatably connected to the rotating seat 11 through a pressure cover rotating shaft. The pressure cover 4 has a pressing position and an opening position relative to the base 1. When the pressure cover 4 is rotated to the pressing position, the lower end of the pressure cover 4 is sealed and abutted against the upper end of the base 1, thereby sealing each reagent tank 21, so that the reagent can flow between the reagent tank 21 and the microchannel of the chip 3 in a sealed environment, avoiding gas or liquid leakage and affecting the use of the microfluidic chip; when the pressure cover 4 is rotated to the opening position, the limit assembly can lock the position of the pressure cover 4 to prevent the pressure cover 4 from rotating.

[0040] Specifically, the limiting assembly of this embodiment includes an elastic push piece 51 and a limiting groove 52, one of which is arranged on the gland 4, and the other is arranged on the rotating seat 11. When the gland 4 rotates to the open position, the elastic push piece 51 can be automatically inserted and clamped in the limiting groove 52, thereby limiting the gland 4 from continuing to rotate. It is simple to operate and reliable to lock, and effectively avoids the gland 4 from automatically pressing down due to gravity and affecting the loading process. When the gland 4 needs to be closed, a certain external force is used to overcome the elastic force of the elastic push piece 51, that is, the elastic push piece 51 can be withdrawn from the limiting groove 52, and the restriction on the gland 4 is released. The microfluidic chip loading device of this embodiment has a compact structure and is easy to use. The operator only needs to add the reagent to the reagent tank 21 and cover the gland 4, and the reagent can be driven by the power source to flow in the reagent tank 21 and the chip 3 to realize the loading process. Optionally, the elastic push member 51 of the present embodiment is a spring pin, which is installed on the inner side of the rotating seat 11, and the limiting groove 52 is arranged on the gland 4 and close to the rear end of the gland 4. The structure is simple and reasonable, and the locking is reliable. Specifically, the rotating seat 11 of the present embodiment includes two support blocks 111 arranged at intervals, and the two support blocks 111 are respectively located on both sides of the gland 4. The two support blocks 111 are both provided with shaft holes 112 for installing the gland shaft; the two support blocks 111 are both provided with mounting holes below the shaft holes 112, and the two spring pins are correspondingly installed in the two mounting holes, so that the gland 4 can be locked and limited from both sides of the gland 4, further improving the position stability of the gland 4 when it is opened.

[0041] In the present embodiment, a plurality of gas source control ports 22 are also provided on the loading platform 2, which are used to control the connection or blocking of the corresponding flow path in the microchannel by pressure gas to complete different operations on the reagent. When the gland 4 is rotated to the pressing position, the gland 4 can seal each gas source control port 22 to prevent gas leakage. It should be noted that the microchannel in the chip 3 has a plurality of branch flow paths, and a membrane valve is respectively provided on each flow path, and the opening or closing of the corresponding membrane valve can be controlled by pressure gas, so as to achieve the connection or blocking of the flow path. The membrane valve belongs to the mature technology in the relevant field, and this embodiment will not be repeated. For example, the microchannel of the chip 3 in the present embodiment can be provided with six flow paths, and six gas source control ports 22 can be provided on the loading platform 2, which are respectively used to control the connection or blocking of the six flow paths.

[0042] Optionally, the reagent tank 21 of the present embodiment includes a sample loading tank 211, a waste liquid tank 212 and a collection tank 213, the sample loading tank 211 is connected to the inlet of the microfluidic channel, the waste liquid tank 212 is connected to the waste liquid outlet of the microfluidic channel, and the collection tank 213 is connected to the collection liquid outlet of the microfluidic channel. The reagent in the sample loading tank 211 can enter the microfluidic channel under the drive of the pressure gas provided by the power source. After sufficient mixing and reaction, the mixed reagent that meets the requirements can flow into the collection tank 213 and be collected, and the mixed reagent that does not meet the requirements can flow into the waste liquid tank 212. Exemplarily, the sample loading tank 211 of the present embodiment is provided with four, which are respectively used to load different reagents, such as oil phase solution, reaction reagent solution, cell solution, cell marker solution, etc. Specifically, the corresponding reagent can be added to each sample loading tank 211 by a pipette gun; the waste liquid tank 212 of the present embodiment is provided with one, and the collection tank 213 is provided with one, so as to realize the screening of the corresponding samples.

[0043] In addition, in the prior art, the sample loading operation of the microfluidic chip is generally performed using a syringe pump or a peristaltic pump. First, a steel needle is inserted into the flow channel of the chip, and then one end of a hose is inserted into the steel needle, and the other end is connected to the outlet of the syringe pump or the peristaltic pump. The connection operation is cumbersome and inefficient.

[0044] In order to solve the above problems, in this embodiment, the inner side of the gland 4 is also provided with a plurality of air inlets 41 which are opposite to each sample adding slot 211 and each gas source control port 22, and a plurality of through-flowing airflow channels are provided in the gland 4, the outlet end of the airflow channel is connected to the corresponding air inlet 41, the inlet end of the airflow channel is located at the rear end of the gland 4, and the inlet end of each airflow channel is respectively sealed and connected to an air pipe 6, and each air pipe 6 is respectively connected to an external air source (such as an air pump) to input pressurized gas into the gland 4. In addition, the gland 4 is also provided with an air outlet 42 which is opposite to the waste liquid slot 212 and the collection slot 213, and the air outlet 42 penetrates the gland 4 and is connected to the external environment. In this embodiment, when loading samples, the pressure gas provided by the external gas source can pass through the trachea 6, the air flow channel in the gland 4, and the air inlet 41 in sequence to enter the sample loading slot 211, so as to push the reagent solution in the sample loading slot 211 into the microchannel of the chip 3; at the same time, the pressure gas provided by the external gas source can also pass through the trachea 6, the air flow channel in the gland 4, and the air inlet 41 in sequence to enter the gas source control port 22 to control the connection or blocking of the corresponding flow path in the microchannel. The above arrangement avoids operations such as inserting needles and inserting tubes on the chip 3, and the connection is convenient and efficient. Optionally, in this embodiment, the trachea 6 is sealed and connected to the inlet end of the air flow channel through a UNF threaded joint 61, which is convenient to connect, compact in structure, good in sealing, and can withstand higher air pressure, thereby ensuring that a sufficiently high pressure is provided to the microfluidic chip. In this embodiment, UNF (United Fine Thread) refers to American fine thread. Illustratively, in this embodiment, ten air flow channels are provided, and ten air pipes 6 are correspondingly provided. The ten air pipes 6 are respectively connected to the four sample adding slots 211 and the six air source control ports 22 in a one-to-one correspondence.

[0045] Furthermore, sealing members are provided between the reagent tank 21 and the gland 4, and between the gas source control port 22 and the gland 4; when the gland 4 is rotated to the pressing position, the gland 4 can be sealed and connected with the reagent tank 21 and the gas source control port 22, thereby preventing gas from leaking between the gland 4 and the sample loading platform 2. Optionally, in this embodiment, a plurality of sealing grooves are provided on the gland 4, and each air inlet 41 and each air outlet 42 are respectively provided in a corresponding sealing groove, and a sealing ring is fixed in each sealing groove; the upper ends of each reagent tank 21 and each gas source control port 22 are convexly provided on the sample loading platform 2, and when the gland 4 is rotated to the pressing position, the upper ends of each reagent tank 21 and each gas source control port 22 can be plugged into the corresponding sealing groove and sealed by a sealing ring. The above arrangement effectively ensures the sealing between the air inlet 41 / air outlet 42 and the reagent tank 21, and the sealing between the air inlet 41 and the air source control port 22, improves the control accuracy of the sample loading process, and increases the reliability of the device. Of course, in other embodiments, the sealing ring can also be fixed on the sample loading platform 2, and arranged around the upper ends of each reagent tank 21 and each air source control port 22, which can also achieve a good sealing effect.

[0046] In this embodiment, the loading platform 2 and the chip 3 are both made of transparent materials, and the pressure cover 4 is also provided with an observation port 43 opposite to the microchannel position of the chip 3, which is used to observe the reaction and mixing of the reagents in the chip 3, so that when a cell sample that meets the requirements is found in the microchannel, the cell sample can be collected in the collection tank 213 in time; or the waste liquid that does not meet the requirements can be discharged into the waste liquid tank 212. Optionally, the loading platform 2 of this embodiment is made of acrylic material, and the chip 3 is made of PDMS (polydimethylsiloxane) material.

[0047] Optionally, the microfluidic chip loading device of the present embodiment also includes an adapter plate 7, which is arranged between the loading platform 2 and the chip 3, and is provided with a plurality of first through holes 71 and a plurality of second through holes 72. The back of the loading platform 2 is provided with a plurality of transfer channels connected to each reagent tank 21, and the first through hole 71 is connected to the corresponding reagent tank 21 through the corresponding transfer channel and the flow path inlet or flow path outlet of the microfluidic channel, and the second through hole 72 is used to connect the gas source control port 22 and the membrane valve interface of the microfluidic channel. The transfer of the loading platform 2 and the chip 3 is realized by the adapter plate 7, which is convenient for the reasonable arrangement of the corresponding flow channels on the loading platform 2 and the chip 3. Optionally, the adapter plate 7 is made of glass material.

[0048] Furthermore, the microfluidic chip loading device of the present embodiment further includes a quick lock assembly, which is used to lock the pressure cover 4 in the pressing position on the base 1. The quick lock assembly includes a first locking member 81 and a second locking member 82, one of which is arranged on the pressure cover 4, and the other of which is arranged on the base 1; when the pressure cover 4 is rotated to the pressing position, the first locking member 81 can be locked with the second locking member 82, so that the pressure cover 4 is sealed and abutted against the upper end of the reagent tank 21. Optionally, the first locking member 81 of the present embodiment is arranged on the pressure cover 4, and includes a rotating part 811 and a locking part 812. The rotating part 811 is rotatably arranged on the pressure cover 4, and the locking part 812 is vertically arranged on the rotating part 811; the second locking member 82 is arranged on the base 1, and the second locking member 82 is provided with a first locking cavity 821 and a second locking cavity 822 that are connected. By rotating the first locking member 81, the locking part 812 can be switched between being movably arranged in the first locking cavity 821 and being locked in the second locking cavity 822.

[0049] The working principle of the microfluidic chip loading device is described below:

[0050] First, add an appropriate amount of reagent to each sample loading groove 211, and place the sample loading platform 2 loaded with reagents into the installation groove on the base 1; then, cover the pressure cover 4 and lock the pressure cover 4 with the base 1 through the quick lock assembly; then, introduce appropriate pressure gas into each air pipe 6 through a pre-set control program to achieve the sample loading operation of the corresponding reagent and the opening and closing control of the corresponding flow path. After the sample is added, the corresponding reagent solution mixes and reacts in the microchannel of the chip 3. The observation or lighting operation of the reagent in the microchannel can be achieved through the observation port 43. In this process, the sample reagent that meets the requirements is selected to flow into the collection groove 213, and the reagent that does not meet the requirements is made to flow into the waste liquid tank 212. After the above operation is completed, open the quick lock assembly, rotate the pressure cover 4 to the open position, and the elastic push member 51 is pushed into the limit groove 52 to prevent the pressure cover 4 from being automatically pressed down due to gravity and affecting the operation.

[0051] Note that the above are only preferred embodiments of the present invention and the technical principles used. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and that various obvious changes, readjustments and substitutions can be made by those skilled in the art without departing from the scope of protection of the present invention. Therefore, although the present invention is described in more detail through the above embodiments, the present invention is not limited to the above embodiments, and may include more other equivalent embodiments without departing from the concept of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. A microfluidic chip loading device, characterized in that: include: A base (1), wherein a rotating base (11) is provided on the base (1); A sample loading platform (2) is installed on the base (1), and a plurality of reagent tanks (21) are arranged on the sample loading platform (2); A chip (3) is arranged on a side of the sample loading platform (2) away from the reagent tank (21), and a microchannel capable of communicating with the reagent tank (21) is arranged in the chip (3); A pressure cover (4) is rotatably connected to the rotating seat (11), and the pressure cover (4) has a pressing position in sealing contact with the base (1) and an opening position open relative to the base (1); The limiting assembly comprises an elastic pushing member (51) and a limiting groove (52), wherein one of the elastic pushing member (51) and the limiting groove (52) is arranged on the pressure cover (4), and the other is arranged on the rotating seat (11); when the pressure cover (4) is rotated to the open position, the elastic pushing member (51) can be inserted into and clamped in the limiting groove (52) to limit the rotation of the pressure cover (4).

2. The microfluidic chip sample loading device according to claim 1, characterized in that: The sample loading platform (2) is also provided with a plurality of gas source control ports (22) for controlling the connection or blocking of the flow path in the microchannel by means of pressure gas.

3. The microfluidic chip loading device according to claim 2, characterized in that: The reagent tank (21) comprises a sample loading tank (211), a waste liquid tank (212) and a collection tank (213); the sample loading tank (211) is connected to the inlet of the microfluidic channel, the waste liquid tank (212) is connected to the waste liquid outlet of the microfluidic channel, and the collection tank (213) is connected to the collection liquid outlet of the microfluidic channel; the reagent in the sample loading tank (211) can enter the microfluidic channel under the drive of the pressure gas, and finally enter the waste liquid tank (212) or the collection tank (213).

4. The microfluidic chip sample loading device according to claim 3, characterized in that: The inner side of the pressure cover (4) is provided with an air inlet (41) which is opposite to the sample adding groove (211) and the air source control port (22); an air flow channel is also provided inside the pressure cover (4); the outlet end of the air flow channel is connected to the air inlet (41); the inlet end of the air flow channel is sealed and connected to the air pipe (6); the air pipe (6) is used to communicate with an external air source; the pressure cover (4) is also provided with an air outlet (42) which is opposite to the waste liquid groove (212) and the collection groove (213).

5. The microfluidic chip sample loading device according to claim 4, characterized in that: The air pipe (6) is sealedly connected to the inlet end of the air flow channel via a UNF threaded joint (61).

6. The microfluidic chip sample loading device according to claim 4, characterized in that: A sealing member is provided between the reagent tank (21) and the pressure cover (4), and between the gas source control port (22) and the pressure cover (4); when the pressure cover (4) is rotated to the pressing position, the pressure cover (4) is sealed and connected to the reagent tank (21) and the gas source control port (22).

7. The microfluidic chip sample loading device according to claim 6, characterized in that: The pressure cover (4) is provided with a plurality of sealing grooves, and the air inlet (41) and the air outlet (42) are respectively arranged in the corresponding sealing grooves; the upper ends of the reagent tank (21) and the air source control port (22) are both protrudingly arranged on the sample loading platform (2) for plugging with the corresponding sealing grooves; the sealing member is a sealing ring, and the sealing ring is fixed in the sealing groove.

8. The microfluidic chip sample loading device according to claim 1, characterized in that: The sample loading platform (2) and the chip (3) are both made of transparent material, and the pressure cover (4) is provided with an observation port (43) for observing the reagent status in the chip (3); and / or, The elastic pushing member (51) is a spring pin, which is installed on the inner side of the rotating seat (11). The limiting groove (52) is arranged on the pressure cover (4) and is close to the rear end of the pressure cover (4).

9. The microfluidic chip sample loading device according to claim 2, characterized in that: The microfluidic chip loading device also includes an adapter plate (7), which is arranged between the loading platform (2) and the chip (3), and is provided with a plurality of first through holes (71) and a plurality of second through holes (72), wherein the first through holes (71) are used to connect the reagent tank (21) with the inlet or outlet of the microchannel, and the second through holes (72) are used to connect the gas source control port (22) with the membrane valve interface of the microchannel.

10. The microfluidic chip sample loading device according to any one of claims 1 to 9, characterized in that: The microfluidic chip loading device also includes a quick-locking component, which includes a first locking member (81) and a second locking member (82), wherein one of the first locking member (81) and the second locking member (82) is arranged on the pressure cover (4), and the other is arranged on the base (1); when the pressure cover (4) is rotated to the pressing position, the first locking member (81) can be locked with the second locking member (82) so that the pressure cover (4) is sealed and abutted against the upper end of the reagent tank (21).

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