Mouse ascites uniform mixing equipment

Abstract

The utility model relates to the technical field of antibody preparation, in particular to mouse ascites mixing equipment which comprises a receiving tank, a feeding barrel, a sample discharging barrel and an air filter, a balancing weight, a temperature sensor, a stirrer and a spraying ball are arranged in the receiving tank. The receiving tank is made of 316L steel, and is provided with a balancing weight, so that the stock of raw materials in the tank body can be effectively detected, the accurate amount of ascites or other components added into the tank body is ensured, and the experiment repeatability and reliability are improved; the tank body is provided with movable pulleys, so that the tank body can be moved to corresponding positions according to different requirements to meet the production requirements; the system design that a CIP system is externally connected for cleaning and sealing the tank body can effectively reduce the risk that a sample is polluted in the operation process, the sterile state of the sample is guaranteed, and through the valve system and the air filter which are correctly connected, the pressure and protection in the tank body can be effectively managed, and the operation safety is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of antibody preparation, in particular to a mouse ascites mixing device. Background Art

[0002] At present, the raw materials are mixed evenly in large-capacity beakers at the laboratory level to mix different batches of raw materials to meet the production needs, and laboratory-level magnetic stirrers are used. At present, industrial-level antibody production teams have high requirements for mouse ascites raw materials, and the demand is large, and multiple batches of raw materials of different volumes need to be mixed. Laboratory-level large-capacity beakers are difficult to meet the demand, and magnetic stirrers are difficult to mix raw materials evenly when the batch is large, which has an adverse effect on quality inspection. Summary of the invention

[0003] The utility model provides a mouse ascites mixing device in order to solve the problems existing in the prior art.

[0004] The technical solutions adopted in this utility model are:

[0005] A mouse ascites mixing device comprises a receiving tank, a feeding barrel, a sample discharging barrel and an air filter; a counterweight block, a temperature sensor, a stirrer and a spray ball are arranged in the receiving tank.

[0006] Furthermore, the receiving tank is provided with two feed ports, and two feed channels are respectively provided between the feed barrel and the receiving tank, one feed channel is used for adding raw materials, and the other feed channel is connected to an external CIP system for cleaning the tank body.

[0007] Furthermore, two valves are respectively provided on the two feed channels.

[0008] Furthermore, a pulley is provided on the bottom bracket of the receiving tank.

[0009] Furthermore, the stirrer is a variable frequency regulator, and different stirring speeds can be set according to actual production conditions.

[0010] Furthermore, the air filter is connected to the receiving tank in a quick-connect chuck manner.

[0011] Furthermore, a valve 5 is connected between the receiving tank and the sample outlet barrel.

[0012] Furthermore, the device is also applicable to other experiments requiring mixing of cell suspensions.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] The receiving tank is made of 316L steel and is equipped with a counterweight to effectively detect the raw material inventory in the tank, ensuring the accuracy of the amount of ascites or other components added to the tank, thereby improving experimental repeatability and reliability; the tank is equipped with a movable pulley, which can move the tank to the corresponding position according to different needs to meet production needs; the system design of an external CIP system for cleaning and sealing the tank can effectively reduce the risk of sample contamination during operation and ensure the sterility of the sample. The correctly connected valve system and air filter can effectively manage the pressure and protection inside the tank and improve operational safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The overall structure diagram of this device DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0017] A mouse ascites mixing device comprises a receiving tank 1, a feed barrel 2, a sample outlet barrel 3, and an air filter 4; a counterweight block 7, a temperature sensor 8, a stirrer 10, and a spray ball 11 are arranged in the receiving tank.

[0018] The receiving tank 1 is provided with two feed ports, and two feed channels are respectively provided between the feed barrel 2 and the receiving tank 1, one feed channel is used for adding raw materials, and the other feed channel is externally connected to the CIP system for cleaning the tank body.

[0019] Two valves 5 are respectively provided on the two feed channels.

[0020] The bottom bracket of the receiving tank is provided with a pulley 6.

[0021] The agitator 10 is a variable frequency regulator, and different stirring speeds can be set according to actual production conditions.

[0022] The air filter 4 is connected to the receiving tank 1 by a quick-connect chuck. Example

[0023] Preparation:

[0024] Check all equipment and utensils to ensure they are clean and sterile.

[0025] Confirm that the air filter 4, counterweight 7, mechanical agitator 10, temperature sensor 8 and manual valve 5 are functioning normally and have been correctly installed.

[0026] Connect your device:

[0027] The air filter 4 is connected to the balance tank through a quick-connect chuck to ensure pressure balance between the inside and outside of the tank.

[0028] The counterweight block 7 is welded to the tank body in preparation for subsequent material weighing.

[0029] A mechanical stirrer 10 is welded to the tank to provide a mixing function.

[0030] A temperature sensor 8 is embedded in the tank body to monitor the temperature of the material in the tank.

[0031] Extraction of ascites:

[0032] Use a sterile syringe and needle to extract ascites samples from the mouse peritoneal cavity and transfer the samples to the pre-prepared equilibrium tank.

[0033] Set the control parameters:

[0034] Make sure that the manual valve 5 is in a closed state to prevent the material from flowing out during the mixing process.

[0035] The temperature value from the temperature sensor 8 is monitored and recorded using appropriate instrumentation to ensure that mixing is performed within the appropriate temperature range.

[0036] Weighing:

[0037] Open the manual valve 5 to start material weighing, and use the counterweight 7 to accurately measure the weight of the material in the tank to ensure accurate subsequent mixing.

[0038] Start stirring:

[0039] Start the mechanical stirrer 10 and set an appropriate stirring speed according to experimental requirements.

[0040] Continue stirring for a while to ensure that the materials are evenly mixed. Depending on the characteristics of the materials, the stirring time can be adjusted according to the actual situation (usually a few minutes to more than ten minutes).

[0041] Monitoring the mixing status:

[0042] During the stirring process, the temperature inside the tank is monitored by the temperature sensor 8 to ensure that it remains within a safe range.

[0043] If uneven mixing or too high temperature is found, adjust the stirring speed appropriately or terminate the experiment.

[0044] End of experiment:

[0045] Stop stirring and close manual valve 5.

[0046] Gently open the air filter 4 to release the internal pressure and ensure safety.

[0047] Sampling and analysis:

[0048] As needed, remove the mixture from the equilibrium tank for subsequent analysis or experiments.

[0049] Record experimental data, including weighing, mixing time, temperature, etc., for subsequent analysis and comparison.

[0050] Cleaning and disinfection:

[0051] After completing the experiment, clean the equipment and work area promptly to ensure that all equipment is properly disposed of to avoid cross contamination.

[0052] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention, and any figure mark in the claims should not be regarded as limiting the claims involved.

Claims

1. A mouse ascites mixing device, characterized in that: The invention comprises a receiving tank (1), a feed barrel (2), a sample discharge barrel (3), and an air filter (4); a counterweight (7), a temperature sensor (8), an agitator (10), and a spray ball (11) are arranged in the receiving tank (1).

2. A mouse ascites mixing device according to claim 1, characterized in that: The receiving tank (1) is provided with two feed ports, and two feed channels are respectively provided between the feed barrel (2) and the receiving tank (1), one feed channel is used for adding raw materials, and the other feed channel is connected to an external CIP system for cleaning the tank body.

3. A mouse ascites mixing device according to claim 1, characterized in that: Two valves (5) are respectively provided on the two feed channels.

4. A mouse ascites mixing device according to claim 1, characterized in that: The bottom bracket of the receiving tank (1) is provided with a pulley (6).

5. A mouse ascites mixing device according to claim 1, characterized in that: The stirrer (10) is a variable frequency regulator, and different stirring speeds can be set according to actual production conditions.

6. A mouse ascites mixing device according to claim 1, characterized in that: The air filter (4) is connected to the receiving tank (1) by means of a quick-connect chuck.

7. A mouse ascites mixing device according to claim 1, characterized in that: A valve (5) is connected between the receiving tank (1) and the sample outlet barrel (3).

8. The mouse ascites mixing device according to claim 1, characterized in that: The device can be used in other biological samples where cell suspension mixing experiments are required.

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