Immune cell sampling device

Abstract

The utility model discloses an immune cell sampling device which comprises an outer cylinder, an inner cylinder is sleeved in the outer cylinder, and the lower end of the inner cylinder is fixed on the outer cylinder; a piston is arranged in the inner cylinder in a sliding mode, the upper end of the piston is coaxially and fixedly connected with a fixing sleeve, a jacking column is installed in the fixing sleeve through threads, and scale marks are arranged on the outer ring of the jacking column in the axial direction; an upper sealing plate is installed at the upper end of the inner cylinder, a vent hole is formed in the middle of the upper sealing plate, and the vent hole of the upper sealing plate is connected with a negative pressure pipe. According to the utility model, after the capacity of a sucked sample is adjusted at one time, the sample can be automatically and quantitatively extracted, the step of checking scales when immune cells are extracted each time is omitted, and the inner cylinder is simple to disassemble and convenient to transfer and clean.

Description

Technical Field

[0001] The utility model relates to an immune cell sampling device, belonging to the technical field of immune cell sampling. Background Art

[0002] Immune cells refer to cells that participate in or are related to immune responses, including lymphocytes, dendritic cells, monocytes / macrophages, granulocytes, mast cells, leukocytes, etc. Immune cells can be divided into many types, and various immune cells play important roles in the human body. Immune cells, commonly known as leukocytes, include innate lymphocytes, various phagocytes, and lymphocytes that can recognize antigens and produce specific immune responses.

[0003] A Chinese utility model patent with announcement number CN214373608U discloses a cell suction device for biological immune cell detection, including a tube body, a holding tube fixedly connected to the top of the tube body, an observation port opened on the side wall of the holding tube, and scale lines engraved on the outer wall of the observation port, a rotating sleeve rotatably connected to the top of the holding tube, and a limit tube is arranged in the holding tube. The utility model rotates the rotating sleeve, the rotating sleeve adjusts the extended length of the limit tube, adjusts the distance between the top of the limit tube and the bottom of the pressure plate, and observes the position of the scale line where the connecting ring is located through the observation port to determine the capacity of the device to absorb the sample.

[0004] The outer wall of the observation port of the above patent is engraved with scale lines to adjust the volume of the sample to be absorbed. However, the scale needs to be checked every time the sample is absorbed, which is troublesome and the internal structure is complicated, making it inconvenient to use. In addition, the tube body is not easy to clean and store.

[0005] In summary, the prior art obviously has inconveniences and defects in practical use, so it is necessary to improve it. Utility Model Content

[0006] The utility model aims at the deficiencies in the background technology and provides an immune cell sampling device, which can automatically extract quantitatively after adjusting the volume of the sample to be absorbed, eliminating the step of checking the scale every time the immune cells are extracted, and the inner cylinder is easy to disassemble, so it is convenient for transfer and cleaning.

[0007] In order to solve the above technical problems, the utility model adopts the following technical solutions:

[0008] An immune cell sampling device comprises an outer cylinder, an inner cylinder is mounted inside the outer cylinder, and the lower end of the inner cylinder is fixed on the outer cylinder;

[0009] A piston is slidably arranged in the inner cylinder, and the upper end of the piston is coaxially fixedly connected with a fixed sleeve, and a top column is installed in the fixed sleeve. The top column is installed in the fixed sleeve through threads, and the outer ring of the top column is provided with scale lines along the axial direction;

[0010] An upper sealing plate is installed at the upper end of the inner cylinder, a vent hole is arranged in the middle of the upper sealing plate, and the vent hole of the upper sealing plate is connected to the negative pressure pipe.

[0011] Furthermore, the upper sealing plate is rotatably mounted on the upper end of the inner cylinder through a thread, and an air nozzle is provided at the air vent on the upper surface of the upper sealing plate.

[0012] Furthermore, the outer cylinder comprises an outer wall and an inner wall, an annular cavity is formed between the outer wall and the inner wall, and a porcelain sleeve is sleeved in the annular cavity.

[0013] Furthermore, an upper cover is provided on the top of the outer cylinder, and the upper cover is installed on the top of the outer cylinder through a threaded connection.

[0014] Furthermore, the lower end of the inner cylinder extends downward to form a threaded fixing section, which passes through the bottom wall of the outer cylinder and extends below the bottom wall. The lower end of the outer cylinder is provided with a locking nut, which cooperates with the outer circle thread of the threaded fixing section.

[0015] Furthermore, a notch is provided on the bottom wall of the outer cylinder, and a positioning protrusion is provided on the bottom of the inner cylinder, and the positioning protrusion cooperates with the notch to prevent the inner cylinder from rotating relative to the outer cylinder.

[0016] Furthermore, the needle connecting section with a tapered tube opening at the lowermost end of the inner tube is used for connecting a needle.

[0017] Furthermore, the inner cylinder is made of stainless steel.

[0018] Compared with the prior art, the utility model has the following advantages after adopting the above technical solution:

[0019] 1. When taking samples, adjust the scale value so that the scale line corresponds to the upper end of the fixed sleeve. The corresponding scale value is equal to the liquid taking capacity of the lower section of the piston of the inner cylinder. After adjusting the volume of the sample to be absorbed once, it can be automatically quantitatively extracted, eliminating the step of checking the scale every time immune cells are extracted. The extraction capacity is fixed and convenient.

[0020] 2. The lower end of the inner cylinder extends downward to form a threaded fixing section, which passes through the bottom wall of the outer cylinder and extends below the bottom wall. A locking nut is provided at the lower end of the outer cylinder, and the locking nut cooperates with the outer circle thread of the threaded fixing section to facilitate the disassembly and assembly of the inner cylinder, thereby facilitating transfer and cleaning.

[0021] The utility model is described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural schematic diagram of the utility model;

[0023] Figure 2It is a structural schematic diagram of the inner cylinder in the utility model.

[0024] In the figure,

[0025] 1-outer cylinder, 101-outer wall, 102-inner wall, 2-porcelain sleeve, 3-inner cylinder, 301-positioning protrusion, 302-threaded fixing section, 4-locking nut, 5-upper cover, 6-piston, 7-fixed sleeve, 8-top column, 9-upper sealing plate, 10-air nozzle. DETAILED DESCRIPTION

[0026] In order to have a clearer understanding of the technical features, purposes and effects of the utility model, the specific implementation methods of the utility model are now described with reference to the accompanying drawings.

[0027] like Figure 1 As shown, the utility model provides an immune cell sampling device, comprising an outer cylinder 1, an inner cylinder 3 is sleeved inside the outer cylinder 1, and the lower end of the inner cylinder 3 is fixed on the outer cylinder 1;

[0028] A piston 6 is slidably arranged in the inner cylinder 3, and a fixed sleeve 7 is coaxially fixedly connected to the upper end of the piston 6, and a top column 8 is installed in the fixed sleeve 7, and the top column 8 is installed in the fixed sleeve 7 by means of threads, and a scale line is arranged along the axial direction on the outer ring of the top column 8, and the top column 8 is rotated to move up and down, so that the scale line corresponds to the upper end of the fixed sleeve 7, and the corresponding scale value is equal to the liquid taking capacity of the lower section of the piston 6 of the inner cylinder 3;

[0029] An upper sealing plate 9 is installed at the upper end of the inner cylinder 3, and a vent hole is provided in the middle of the upper sealing plate 9. When extracting samples, the vent hole of the upper sealing plate 9 is connected to the negative pressure tube, so that the piston 6 moves upward from the bottom of the inner cylinder 3. When the top of the top column 8 is at the vent hole, the capacity of the extracted sample is equal to the scale value set on the top column 8.

[0030] The upper sealing plate 9 is rotatably mounted on the upper end of the inner tube 3 by means of threads, and an air nozzle 10 is provided at the air vent on the upper surface of the upper sealing plate 9 .

[0031] The outer cylinder 1 includes an outer wall 101 and an inner wall 102, and an annular cavity is formed therebetween. A porcelain sleeve 2 is mounted in the annular cavity. Ceramics have a large specific heat capacity and a strong heat absorption capacity. When refrigeration is required, the heat in the immune cells in the inner cylinder 3 is quickly transferred to the porcelain sleeve 2, thereby improving the refrigeration effect.

[0032] An upper cover 5 is provided on the top of the outer cylinder 1 , and the upper cover 5 is installed on the top of the outer cylinder 1 through threaded connection.

[0033] like Figure 2The lower end of the inner tube 3 extends downward to form a threaded fixing section 302, and the threaded fixing section 302 passes through the bottom wall of the outer tube 1 and extends below the bottom wall. The lower end of the outer tube 1 is provided with a locking nut 4, and the locking nut 4 cooperates with the outer circle thread of the threaded fixing section 302.

[0034] A notch is provided on the bottom wall of the outer cylinder 1 , and a positioning protrusion 301 is provided on the bottom of the inner cylinder 3 . The positioning protrusion 301 cooperates with the notch to prevent the inner cylinder 3 from rotating relative to the outer cylinder 1 .

[0035] The needle connecting section with a tapered tube opening at the lowermost end of the inner tube 3 is used for connecting a needle.

[0036] The inner cylinder 3 is made of stainless steel, which is convenient for cleaning and heat transfer.

[0037] The specific working principle of the utility model:

[0038] When taking samples, adjust the scale value so that the scale line corresponds to the upper end of the fixed sleeve 7, and the corresponding scale value is equal to the liquid taking capacity of the lower section of the piston 6 of the inner cylinder 3. After adjusting the volume of the sample to be sucked once, it can be automatically quantitatively extracted, eliminating the step of checking the scale every time the immune cells are extracted, and the extraction capacity is fixed and convenient. In addition, the lower end of the inner cylinder 3 extends downward to form a threaded fixed section 302, and the threaded fixed section 302 passes through the bottom wall of the outer cylinder 1 and extends below the bottom wall. The lower end of the outer cylinder 1 is provided with a locking nut 4, and the locking nut 4 cooperates with the outer circle thread of the threaded fixed section 302, which is convenient for the disassembly and assembly of the inner cylinder 3, thereby facilitating transfer and cleaning.

[0039] The above is an example of the best implementation of the utility model, and the parts not described in detail are common knowledge of ordinary technicians in this field. The protection scope of the utility model is based on the content of the claims, and any equivalent transformation based on the technical inspiration of the utility model is also within the protection scope of the utility model.

Claims

1. An immune cell sampling device, characterized in that: It comprises an outer cylinder (1), an inner cylinder (3) is sleeved inside the outer cylinder (1), and the lower end of the inner cylinder (3) is fixed on the outer cylinder (1); A piston (6) is slidably disposed in the inner cylinder (3), the upper end of the piston (6) is coaxially fixedly connected with a fixed sleeve (7), a top column (8) is installed in the fixed sleeve (7), the top column (8) is installed in the fixed sleeve (7) through threads, and the outer ring of the top column (8) is provided with scale lines along the axial direction; An upper sealing plate (9) is installed at the upper end of the inner cylinder (3), a vent hole is arranged in the middle of the upper sealing plate (9), and the vent hole of the upper sealing plate (9) is connected to the negative pressure pipe.

2. An immune cell sampling device as claimed in claim 1, characterized in that: The upper sealing plate (9) is rotatably mounted on the upper end of the inner cylinder (3) by means of a thread, and an air nozzle (10) is provided at the air vent on the upper surface of the upper sealing plate (9).

3. The immune cell sampling device according to claim 1, characterized in that: The outer cylinder (1) comprises an outer wall (101) and an inner wall (102), an annular cavity is formed between the two, and a porcelain sleeve (2) is sleeved in the annular cavity.

4. An immune cell sampling device as claimed in claim 3, characterized in that: An upper cover (5) is provided on the top of the outer cylinder (1), and the upper cover (5) is installed on the top of the outer cylinder (1) through a threaded connection.

5. The immune cell sampling device according to claim 1, characterized in that: The lower end of the inner cylinder (3) extends downward to form a threaded fixing section (302), which passes through the bottom wall of the outer cylinder (1) and extends below the bottom wall. The lower end of the outer cylinder (1) is provided with a locking nut (4), which cooperates with the outer circle thread of the threaded fixing section (302).

6. An immune cell sampling device as claimed in claim 5, characterized in that: A notch is provided on the bottom wall of the outer cylinder (1), and a positioning protrusion (301) is provided on the bottom of the inner cylinder (3). The positioning protrusion (301) cooperates with the notch to prevent the inner cylinder (3) from rotating relative to the outer cylinder (1).

7. The immune cell sampling device according to claim 1, characterized in that: The inner tube (3) has a needle connecting section with a gradually tapering tube opening at the bottom end, and is used for connecting a needle.

8. The immune cell sampling device according to claim 1, characterized in that: The inner cylinder (3) is made of stainless steel.

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