Liquid supplementing device for cell culture

Abstract

The utility model discloses a fluid infusion device for cell culture, and relates to the technical field of cell culture. The device comprises a bottom plate, the upper side of the bottom plate is fixedly connected with an incubator, the upper side of the incubator is provided with an infusion box, one side of the infusion box is fixedly connected with a connecting pipe, the connecting pipe is provided with a control valve, the lower end of the connecting pipe is fixedly connected with a liquid pumping pipe, and the inner wall of the infusion box is in sliding fit with a sealing plate. By arranging the driving mechanism, when a culture solution in the infusion box needs to be supplemented, the rotating disc is rotated to drive the worm to rotate, the threaded rod is rotated under the transmission of the worm gear meshed with the worm, the threaded rod rotates to drive the threaded sleeve to ascend, and the sealing plate moves upwards under the action of the connecting piece; the culture solution in the culture solution bottle is extracted through the solution extraction pipe at one end of the connecting pipe, so that the convenience of supplementing the culture solution to the infusion box is improved, the culture solution in the infusion box is prevented from being exposed in the air, and the risk that the culture solution is polluted is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and specifically relates to a liquid replenishing device for cell culture. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions.

[0003] A Chinese patent with application number CN202222076975.5 discloses a fluid replenishment device for cell culture, including a table top, a fixed plate fixedly connected to the left upper portion of the table top, a fixed sleeve fixedly connected to the middle right portion of the fixed plate, a hole opened on the lower side of the fixed sleeve, a liquid storage cylinder slidably connected to the inner wall of the fixed sleeve, and an extrusion component threadedly connected to the upper end of the liquid storage cylinder.

[0004] The above scheme drives the screw to rotate by driving the motor, and uses the screw to drive the threaded sleeve to move to drive the rubber plate to push the culture solution into the incubator. However, in actual use, when the culture solution in the liquid storage cylinder is used up, it is necessary to open the sealing cover to add or replace the culture solution inside. After the sealing cover is opened, the inner wall of the liquid storage cylinder is exposed to the air, and the culture solution contacts with the outside air and is easily contaminated, thereby affecting the cell culture effect. In addition, the addition of culture solution requires the disassembly of the extrusion component, which is relatively cumbersome.

[0005] In view of this, the present utility model is proposed. Utility Model Content

[0006] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a fluid replenishing device for cell culture.

[0007] In order to solve the above technical problems, the basic concept of the technical solution adopted by the utility model is:

[0008] A cell culture fluid replenishment device comprises a bottom plate, a culture box is fixedly connected to the upper side of the bottom plate, an infusion box is installed on the upper side of the culture box, a connecting pipe is fixedly connected to one side of the infusion box, a control valve is installed on the connecting pipe, a liquid extraction pipe is fixedly connected to the lower end of the connecting pipe, a sealing plate is slidably matched on the inner wall of the infusion box, a mounting groove is opened on one side of the inner wall of the infusion box, and a driving mechanism is arranged on the inner wall of the mounting groove;

[0009] The driving mechanism includes a threaded rod rotatably engaged with the lower side of the inner wall of the mounting groove, a threaded sleeve threadedly engaged with the threaded rod and slidingly engaged with the mounting groove, a worm wheel mounted on the threaded rod, a worm gear meshingly engaged with the worm gear rotatably engaged with one side of the inner wall of the mounting groove, a turntable fixedly connected to one end of the worm gear, and a connecting piece mounted between the sealing plate and the threaded sleeve.

[0010] Optionally, a mounting plate is installed on the upper side of the bottom plate, a strip groove is opened on one side of the mounting plate, a slider is slidably matched on the inner wall of the strip groove, a fixing plate is fixedly connected to one side of the slider, a placement plate is fixedly connected to one side of the fixing plate, and a limiting ring is installed on one side of the fixing plate. The placement plate and the limiting ring are provided to limit the position of the culture fluid bottle.

[0011] Optionally, a T-shaped plate is slidably and elastically matched on one side of the slider, and two anti-slip plates matched with the mounting plate are fixedly connected to one side of the T-shaped plate. The anti-slip plate is provided to increase the friction between the slider and the mounting plate to prevent the slider from sliding downward under the action of gravity.

[0012] Optionally, a rectangular groove is provided on one side of the incubator, and a sealing door is rotatably provided on the inner wall of the rectangular groove. The sealing door is provided to facilitate operation inside the incubator.

[0013] Optionally, a dovetail groove is provided on the upper side of the bottom plate, and a dovetail block fixedly connected to the mounting plate is slidably matched on the inner wall of the dovetail groove. The dovetail groove and the dovetail block are provided to limit and fix the mounting plate, so that the mounting plate can drive the culture liquid bottle to move.

[0014] Optionally, a liquid level window is installed on one side of the infusion box, a scale line matching the liquid level window is installed on one side of the infusion box, and a pressure regulating valve is installed on one side of the incubator. By setting the scale line and the liquid level window, it is convenient to observe the liquid level inside the infusion box, and by setting the pressure regulating valve, it is convenient to adjust the pressure inside the incubator, so as to facilitate the replenishment of the culture fluid.

[0015] After adopting the above technical solution, the utility model has the following beneficial effects compared with the prior art. Of course, any product implementing the utility model does not necessarily need to achieve all the advantages described below at the same time:

[0016] By setting up a driving mechanism, when the culture fluid in the infusion box needs to be supplemented, the turntable is rotated to drive the worm to rotate, and the threaded rod is rotated under the transmission of the worm gear meshing therewith, and the rotation of the threaded rod drives the threaded sleeve to rise, and the sealing plate is moved upward under the action of the connecting piece, and the culture fluid in the culture fluid bottle is extracted through the extraction tube at one end of the connecting tube, thereby improving the convenience of replenishing the culture fluid in the infusion box, avoiding the exposure of the culture fluid inside the infusion box to the air, and reducing the risk of the culture fluid being contaminated.

[0017] The specific implementation of the utility model is further described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings described below are only some embodiments. For those skilled in the art, other drawings can be obtained based on these drawings without creative work. In the drawings:

[0019] Figure 1 This is a schematic diagram of the three-dimensional structure of an embodiment of the utility model;

[0020] Figure 2 It is a front cross-sectional structural schematic diagram of an embodiment of the utility model;

[0021] Figure 3 for Figure 2 Schematic diagram of the structure at A in the middle;

[0022] Figure 4 This is a schematic diagram of a worm connection structure according to an embodiment of the utility model;

[0023] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0024] 1. Base plate; 2. Incubator; 3. Infusion box; 4. Connecting pipe; 5. Control valve; 6. Extraction pipe; 7. Sealing plate; 8. Mounting groove; 9. Driving mechanism; 91. Threaded rod; 92. Threaded sleeve; 93. Worm gear; 94. Worm; 95. Turntable; 96. Connecting piece; 10. Mounting plate; 11. Strip groove; 12. Sliding block; 13. Fixing plate; 14. Placement plate; 15. Limiting ring; 16. T-shaped plate; 17. Anti-skid plate; 18. Rectangular groove; 19. Sealing door; 20. Dovetail groove; 21. Dovetail block; 22. Liquid level window; 23. Graduation mark; 24. Pressure regulating valve.

[0025] It should be noted that these drawings and textual descriptions are not intended to limit the conceptual scope of the present invention in any way, but rather to illustrate the concept of the present invention for those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION

[0026] The utility model will now be further described in detail with reference to the accompanying drawings.

[0027] See also Figure 1-4 As shown, in this embodiment, a fluid replenishing device for cell culture is provided, comprising a bottom plate 1, a culture box 2 is fixedly connected to the upper side of the bottom plate 1, an infusion box 3 is installed on the upper side of the culture box 2, a connecting pipe 4 is fixedly connected to one side of the infusion box 3, a control valve 5 is installed on the connecting pipe 4, a liquid extraction pipe 6 is fixedly connected to the lower end of the connecting pipe 4, a sealing plate 7 is slidably matched on the inner wall of the infusion box 3, a mounting groove 8 is opened on one side of the inner wall of the infusion box 3, and a driving mechanism 9 is arranged on the inner wall of the mounting groove 8;

[0028] The driving mechanism 9 includes a threaded rod 91 rotatably engaged with the lower side of the inner wall of the mounting groove 8, a threaded sleeve 92 threadedly engaged with the threaded rod 91 and slidingly engaged with the mounting groove 8, a worm gear 93 is mounted on the threaded rod 91, a worm 94 rotatably engaged with one side of the inner wall of the mounting groove 8 and meshingly connected with the worm gear 93, a turntable 95 fixedly connected to one end of the worm gear 94, and a connecting piece 96 mounted between the sealing plate 7 and the threaded sleeve 92.

[0029] The application of one aspect of this embodiment is as follows: when in use, when the infusion box 3 needs to be replenished, the culture fluid bottle to be replenished is placed in the groove on the placement plate 14 through the limiting ring 15, the mounting plate 10 is slid to the bottom of the liquid extraction tube 6, the T-shaped plate 16 is pulled to separate the anti-slip plate 17 from the mounting plate 10, the slider 12 is pushed upward, the culture fluid bottle is driven to cooperate with the liquid extraction tube 6, the turntable 95 is rotated, the turntable 95 drives the worm 94 to rotate, and the threaded rod 91 is rotated under the transmission of the worm gear 93 meshing therewith, and the threaded rod 91 rotates, and the threaded sleeve 92 rises when the threaded rod 91 rotates, and the sealing plate 7 is moved upward under the action of the connecting piece 96, and the culture fluid in the culture fluid bottle is extracted through the liquid extraction tube 6 at one end of the connecting tube 4. When the extraction is completed, the mounting plate 10 is reset;

[0030] When the culture fluid in the incubator 2 needs to be supplemented, the turntable 95 is rotated, and the turntable 95 drives the worm 94 to rotate. Under the transmission of the worm gear 93 meshing therewith, the threaded rod 91 is rotated, and the threaded sleeve 92 is lowered by the rotation of the threaded rod 91. Under the action of the connecting piece 96, the sealing plate 7 is moved downward to squeeze and transport the culture fluid in the infusion box 3, and the input amount is controlled according to the scale line 23 and the liquid level window 22.

[0031] By setting up the driving mechanism 9, when the culture fluid in the infusion box 3 needs to be supplemented, the turntable 95 is rotated to drive the worm 94 to rotate, and the threaded rod 91 is rotated under the transmission of the worm gear 93 meshing therewith, and the rotation of the threaded rod 91 drives the threaded sleeve 92 to rise, and under the action of the connecting piece 96, the sealing plate 7 is moved upward, and the culture fluid in the culture fluid bottle is extracted through the extraction tube 6 at one end of the connecting tube 4, thereby improving the convenience of supplementing the culture fluid in the infusion box 3, avoiding the exposure of the culture fluid inside the infusion box 3 to the air, and reducing the risk of the culture fluid being contaminated.

[0032] A mounting plate 10 is mounted on the upper side of the bottom plate 1 of the present embodiment, a strip groove 11 is formed on one side of the mounting plate 10, a slider 12 is slidably fitted on the inner wall of the strip groove 11, a fixing plate 13 is fixedly connected to one side of the slider 12, a placement plate 14 is fixedly connected to one side of the fixing plate 13, a limiting ring 15 is mounted on one side of the fixing plate 13; the placement plate 14 and the limiting ring 15 are provided to limit the position of the culture fluid bottle.

[0033] One side of the slider 12 of the present embodiment slides and elastically cooperates with a T-shaped plate 16, and one side of the T-shaped plate 16 is fixedly connected with two anti-skid plates 17 that cooperate with the mounting plate 10; by providing the anti-skid plate 17, the friction between the slider 12 and the mounting plate 10 is increased to prevent the slider 12 from sliding downward under the action of gravity.

[0034] A rectangular groove 18 is provided on one side of the incubator 2 of the present embodiment, and a sealing door 19 is rotatably provided on the inner wall of the rectangular groove 18 . The sealing door 19 facilitates operation on the inside of the incubator 2 .

[0035] A dovetail groove 20 is provided on the upper side of the base plate 1 of the present embodiment, and the inner wall of the dovetail groove 20 is slidably fitted with a dovetail block 21 fixedly connected to the mounting plate 10; by providing the dovetail groove 20 and the dovetail block 21, the mounting plate 10 is limited and fixed, so that the mounting plate 10 can drive the culture liquid bottle to move.

[0036] In this embodiment, a liquid level window 22 is installed on one side of the infusion box 3, a scale line 23 matching the liquid level window 22 is installed on one side of the infusion box 3, and a pressure regulating valve 24 is installed on one side of the incubator 2; by setting the scale line 23 and the liquid level window 22, it is convenient to observe the liquid level inside the infusion box 3, and by setting the pressure regulating valve 24, it is convenient to adjust the pressure inside the incubator 2, so as to facilitate the replenishment of the culture fluid.

[0037] The present invention is not limited to the above-mentioned implementation modes. Anyone should be aware of the structural changes made under the inspiration of the present invention. Any technical solution that is the same or similar to the present invention falls within the protection scope of the present invention. The technology, shape, and structure that are not described in detail in the present invention are all known technologies.

Claims

1. A fluid replenishing device for cell culture, characterized in that: include: A bottom plate (1), a culture box (2) is fixedly connected to the upper side of the bottom plate (1), an infusion box (3) is installed on the upper side of the infusion box (2), a connecting pipe (4) is fixedly connected to one side of the infusion box (3), a control valve (5) is installed on the connecting pipe (4), a liquid extraction pipe (6) is fixedly connected to the lower end of the connecting pipe (4), a sealing plate (7) is slidably matched on the inner wall of the infusion box (3), a mounting groove (8) is opened on one side of the inner wall of the infusion box (3), and a driving mechanism (9) is arranged on the inner wall of the mounting groove (8); The driving mechanism (9) comprises a threaded rod (91) rotatably engaged with the lower side of the inner wall of the mounting groove (8), a threaded sleeve (92) threadedly engaged with the threaded rod (91) and slidably engaged with the mounting groove (8), a worm wheel (93) mounted on the threaded rod (91), a worm (94) rotatably engaged with one side of the inner wall of the mounting groove (8) and meshingly connected with the worm wheel (93), a rotating disk (95) fixedly connected to one end of the worm (94), and a connecting piece (96) mounted between the sealing plate (7) and the threaded sleeve (92).

2. A fluid replenishing device for cell culture according to claim 1, characterized in that: A mounting plate (10) is mounted on the upper side of the bottom plate (1), a strip groove (11) is formed on one side of the mounting plate (10), a slider (12) is slidably fitted on the inner wall of the strip groove (11), a fixing plate (13) is fixedly connected to one side of the slider (12), a placement plate (14) is fixedly connected to one side of the fixing plate (13), and a limiting ring (15) is mounted on one side of the fixing plate (13).

3. A fluid replenishing device for cell culture according to claim 2, characterized in that: One side of the slide block (12) is slidably and elastically matched with a T-shaped plate (16), and one side of the T-shaped plate (16) is fixedly connected with two anti-slip plates (17) matched with the mounting plate (10).

4. A fluid replenishing device for cell culture according to claim 1, characterized in that: A rectangular groove (18) is provided on one side of the incubator (2), and a sealing door (19) is rotatably matched on the inner wall of the rectangular groove (18).

5. A fluid replenishing device for cell culture according to claim 2, characterized in that: A dovetail groove (20) is provided on the upper side of the bottom plate (1), and a dovetail block (21) fixedly connected to the mounting plate (10) is slidably matched on the inner wall of the dovetail groove (20).

6. The cell culture fluid replenishing device according to claim 1, characterized in that: A liquid level window (22) is installed on one side of the infusion box (3), a scale line (23) matching the liquid level window (22) is installed on one side of the infusion box (3), and a pressure regulating valve (24) is installed on one side of the incubator (2).

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