TMAO negative sample preparation device

Abstract

The utility model discloses a TMAO negative sample preparation device, which relates to the technical field of medical instruments and comprises a cylinder, one end of the cylinder is fixedly communicated with a suction tube, and the other end of the cylinder is provided with an opening; the extrusion bag is arranged at the middle position of the outer wall of the barrel body, and the extrusion bag is communicated with the inner cavity of the barrel body through a through hole; the filtering part is arranged in an inner cavity at one end of the opening of the cylinder body; and the cryopreservation tube is detachably mounted at the opening end of the cylinder body and is used for storing the sample filtered by the filtering part. The device is of an integrated structure, sampling, filtering and storing of samples can be completed only through the device, operation is more convenient, and efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, and in particular to a TMAO negative sample preparation device. Background Art

[0002] TMAO is a metabolite, the full name is trimethylamine N-oxide, which is a substance produced by the metabolism of N-methylamine (TMA) in food by intestinal bacteria in the intestine. TMAO can enter the human body through diet and is mainly found in certain animal foods, such as red meat and seafood. High TMAO levels are associated with the occurrence of cardiovascular diseases, obesity, inflammation and other diseases. Studies have shown that TMAO may play a certain role in promoting the occurrence and development of arteriosclerosis and cardiovascular diseases.

[0003] In recent years, scholars in related fields have shown that the increase in TMAO content can cause a series of diseases, such as inhibiting the expression of Cyp7a1, causing cholesterol accumulation in cells, and ultimately increasing the formation of atherosclerotic plaques; activating the scavenger receptors of macrophage foam cells, inhibiting the transport of cholesterol, leading to atherosclerosis; increasing the activation of NFKB and NL3 inflammasomes in endothelial cells, the expression of adhesion proteins, inhibiting NO, leading to heart failure and kidney disease; increasing the release of calcium in thrombin induced by thrombin or ADP agonists, activating platelets, and promoting thrombosis. Therefore, if the TMAO content level in the body can be accurately detected, it will be of great significance for the prevention and treatment of various diseases.

[0004] Due to the complexity of the matrix in biological samples and the large number of interfering factors in the detection process, negative control samples must be used for background subtraction. Currently, there is a lack of TMAO negative sample preparation devices on the market. Among them, the Chinese patent with authorization announcement number CN209727566U proposes a TMAO negative sample preparation device, which is inconvenient to use and requires the use of multiple instruments. Utility Model Content

[0005] The utility model aims to provide a TMAO negative sample preparation device, which solves the problem that the existing TMAO negative sample preparation device is inconvenient to use and needs to use multiple instruments.

[0006] The utility model solves the above technical problems through the following technical solutions, and the utility model includes:

[0007] A cylinder, one end of which is connected and fixed with a suction tube, and the other end of which has an opening;

[0008] An extrusion bag, which is arranged in the middle of the outer wall of the cylinder, and the extrusion bag is connected with the inner cavity of the cylinder through a through hole;

[0009] A filter unit is disposed in the inner cavity of the open end of the cylinder; and

[0010] The cryotube is detachably mounted on the open end of the cylinder and is used to store samples filtered by the filter unit.

[0011] Preferably, the interior of the cylinder has a first inner cavity, a second inner cavity and a third inner cavity in sequence, and the first inner cavity is close to one end of the suction tube.

[0012] Preferably, the outer wall of the first inner cavity is provided with a scale, and the scale is arranged along the axial direction of the cylinder.

[0013] Preferably, the filter part is arranged in the third inner cavity, and the filter part includes two filter screens fixed in the third inner cavity, a plurality of fillers are filled between the two filter screens, and the outer surfaces of the plurality of fillers are adsorbed with Reiner's salt.

[0014] Preferably, the open end of the cylinder is processed with an external thread, and the external thread is threadedly connected to a second closing cover.

[0015] Preferably, the inner surface of the open end of the cryotube is threadedly connected to the external thread.

[0016] Preferably, the end of the suction tube is detachably connected with a first closing cover, and the first closing cover is connected to the end of the suction tube by threading or plugging.

[0017] Preferably, the number of each extrusion bag is 1-2.

[0018] Compared with the prior art, the beneficial effect of the present invention is that the present invention is an integrated structure, and only the device is needed to complete the sampling, filtering and storage of samples, making the operation more convenient and increasing the efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0020] Figure 2 for Figure 1 A schematic diagram of a half-section three-dimensional structure;

[0021] Figure 3 for Figure 1 A schematic diagram of a main cross-sectional structure;

[0022] Figure 4 Schematic diagram of the structure after the cryopreservation tube is installed.

[0023] The numbers in the figure represent:

[0024] 11-first inner cavity; 12-second inner cavity; 13-third inner cavity; 14-external thread; 2-extrusion bag; 21-through hole; 31-suction tube; 32-first closing cover; 4-scale; 5-second closing cover; 6-filter part; 61-filter screen; 7-cryostorage tube. DETAILED DESCRIPTION

[0025] The above and other technical features and advantages of the present invention are described in more detail below in conjunction with the accompanying drawings.

[0026] This embodiment provides a technical solution: a TMAO negative sample preparation device, such as Figure 1-4 As shown, it includes a cylinder, an extrusion bag 2, a filter part 6 and a cryopreservation tube 7.

[0027] One end of the cylinder is a closed end, and the other end is an open end. It is made of transparent material to facilitate observation of the actual situation of the sample inside. The cylinder is a long structure, and the specific size is set according to needs. The closed end of the cylinder is connected and fixed with a suction tube 31, and the diameter of the suction tube 31 is smaller than the diameter of the cylinder. In order to avoid external infection, a first closed cover 32 is detachably connected to the end of the suction tube 31, that is, the end of the suction tube 31 is covered and the suction tube 31 is closed. Specifically, the first closed cover 32 is connected or plugged with the end of the suction tube 31 by threading, which is convenient for use.

[0028] The interior of the cylinder has a first inner cavity 11, a second inner cavity 12 and a third inner cavity 13 in sequence, and the first inner cavity 11 is an end close to the suction tube 31, wherein the first inner cavity 11 is an area for temporarily storing samples when sucking samples; the second inner cavity 12 is an area for temporarily storing samples when filtering samples; and the third inner cavity 13 is used to place the filter part 6.

[0029] Furthermore, in order to facilitate medical personnel to quantify the volume of the sample taken, a scale 4 is provided on the outer wall of the first inner cavity 11, and the scale 4 is arranged along the axial direction of the cylinder. The scale 4 can show how much volume of the sample is stored in the first inner cavity 11 when taking the sample.

[0030] Furthermore, the squeezing capsule 2 is arranged in the middle position of the outer wall of the cylinder, that is, the position of the second inner cavity 12. The squeezing capsule 2 is made of rubber material. When the squeezing capsule 2 is squeezed, it deforms inward. After the squeezing capsule 2 is released, the squeezing capsule 2 will recover to its original shape due to its own elasticity. The number of each squeezing capsule 2 is 1-2. In this embodiment, the number of squeezing capsules 2 is 2, and the two squeezing capsules 2 are symmetrically arranged. The squeezing capsule 2 is connected to the inner cavity of the cylinder through a through hole 21. The number of through holes 21 located in each squeezing capsule 2 is 2, and the two through holes 21 are respectively located near the upper and lower sides of the squeezing capsule 2 to prevent the sample from entering the squeezing capsule 2 and not being discharged.

[0031] Furthermore, the filter section 6 is arranged in the inner cavity at one end of the opening of the cylinder, that is, in the third inner cavity 13, and the filter section 6 includes two filter screens 61 fixed in the third inner cavity 13, and a plurality of fillers are filled between the two filter screens 61, and the outer surfaces of the plurality of fillers are adsorbed with Reinle's salt. When the sample flows through the filter section 6, the precipitate generated by the TMAO in the sample and the Reinle's salt reagent is retained on the surface of the filter section 6, thereby removing the TMAO in the sample and obtaining a TMAO biological negative sample close to the composition of the plasma or urine sample to be tested.

[0032] The cryogenic tube 7 is detachably mounted on the open end of the cylinder body, and is used to store samples filtered through the filter portion 6. The open end of the cylinder body is processed with an external thread 14, and the inner surface of the open end of the cryogenic tube 7 is threadedly connected to the external thread 14. At the same time, the outer surface of the open end of the cryogenic tube 7 is processed with an external thread for connecting to a sealing cover to seal the cryogenic tube 7.

[0033] The external thread 14 is threadably connected with a second closing cover 5 , and the opening end of the cylinder is sealed by the second closing cover 5 before use.

[0034] When in use, first unscrew the second closing cover 5, screw on the cryopreservation tube 7 and fix it with the external thread 14, and remove the first closing cover 32, then hold the cylinder body with one end of the suction tube 31 facing downward, squeeze the squeezing bag 2 by hand to deform and accumulate force, and squeeze out some of the air in the cylinder body, insert the end of the suction tube 31 into the sample, release the squeezing bag 2 by hand, and the squeezing bag 2 will deform and return to its original shape. Under the action of air pressure, the sample will enter the first inner cavity 11 through the suction tube 31. Medical personnel can judge the volume of the extracted sample based on the scale 4, and select the appropriate volume sample based on this, and then turn the cylinder body upside down so that the suction tube 31 is facing upward, the sample in the first inner cavity 11 will flow into the second inner cavity 12, and gradually enter into the third inner cavity 13 through leakage, and the precipitate generated by the TMAO in the sample and the Reinecke salt reagent is retained on the surface of the filter 6, thereby removing the TMAO in the sample and preparing a TMAO biological negative sample with a composition close to that of the plasma or urine sample to be tested. Then the cryopreservation tube 7 can be removed and sealed by the sealing cover, and then placed in a refrigerator for refrigeration; in addition, the suction tube 31 can be blocked by the first closing cover 32, and the speed of sample penetration can be increased by manually squeezing the squeezing bag 2 to improve efficiency.

[0035] The above are only preferred embodiments of the present invention, which are only illustrative and not restrictive. Those skilled in the art understand that many changes, modifications, and even equivalences may be made to the present invention within the spirit and scope defined by the claims of the present invention, but they will all fall within the scope of protection of the present invention.

Claims

1. A TMAO negative sample preparation device, characterized in that: include: A cylinder, one end of which is connected and fixed with a suction tube (31), and the other end of which has an opening; An extrusion bag (2), the extrusion bag (2) being arranged at a middle position of the outer wall of the cylinder, and the extrusion bag (2) being connected to the inner cavity of the cylinder through a through hole (21); A filter part (6), wherein the filter part (6) is arranged in the inner cavity of one open end of the cylinder; as well as A cryotube (7) is detachably mounted on the open end of the cylinder and is used to store samples filtered by the filter unit (6).

2. The TMAO negative sample preparation device according to claim 1, characterized in that: The interior of the cylinder has a first inner cavity (11), a second inner cavity (12) and a third inner cavity (13) in sequence, and the first inner cavity (11) is an end close to the suction tube (31).

3. The TMAO negative sample preparation device according to claim 2, characterized in that: The outer wall of the first inner cavity (11) is provided with a scale (4), and the scale (4) is arranged along the axial direction of the cylinder.

4. The TMAO negative sample preparation device according to claim 3, characterized in that: The filter part (6) is arranged in the third inner cavity (13), and the filter part (6) comprises two filter screens (61) fixed in the third inner cavity (13), a plurality of fillers are filled between the two filter screens (61), and the outer surfaces of the plurality of fillers are adsorbed with Reiner's salt.

5. The TMAO negative sample preparation device according to claim 1, characterized in that: The open end of the cylinder is processed with an external thread (14), and the external thread (14) is threadedly connected with a second closing cover (5).

6. The TMAO negative sample preparation device according to claim 5, characterized in that: The inner surface of the open end of the freezing tube (7) is threadedly connected to the external thread (14).

7. The TMAO negative sample preparation device according to claim 1, characterized in that: The end of the suction tube (31) is detachably connected to a first closing cover (32), and the first closing cover (32) is connected to the end of the suction tube (31) by threading or plugging.

8. The TMAO negative sample preparation device according to claim 1, characterized in that: The number of each extrusion bag (2) is 1-2.

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