Multi-index flow antibody incubation device

Abstract

The utility model relates to the technical field of flow cytometry, and discloses a multi-index flow cytometry antibody incubation device which is characterized by comprising an incubation tube body, a first partition plate is rotatably arranged on the inner wall of the incubation tube body, the incubation tube body is divided into a first cavity and a second cavity by the first partition plate, and the first cavity is communicated with the second cavity. A plurality of through holes are formed in the first partition plate; a sample chamber and a plurality of shunt pipes are arranged in the first cavity, a plurality of shunt ports are formed in the sample chamber, one end of each shunt pipe is communicated with the corresponding shunt port, and the other end of each shunt pipe extends to the first partition plate; a plurality of first partition plates are arranged in the first cavity, a plurality of second partition plates are arranged in the second cavity, the second cavity is divided into a plurality of incubation chambers by the second partition plates, the incubation chambers can be communicated with the shunt pipes or closed by rotating the first partition plates, and the incubation device can realize simultaneous incubation of multiple indexes.

Description

Technical Field

[0001] The utility model relates to the technical field of flow cell detection, in particular to a multi-index flow antibody incubation device. Background Art

[0002] In cell biology, flow cytometry is a highly versatile application that allows analysis of cell subpopulation phenotypes and cell signaling. Cell detection is based on the different proteins expressed on the surface of different cell types. Antibodies can specifically target these proteins and distinguish cell types through fluorescent dyes bound to the antibodies.

[0003] Antibodies are unique protein molecules that can recognize and bind to foreign invading substances (antigens) to provide immune protection. Incubating antibodies is a common experimental method that can be used in biomedical research, disease diagnosis and treatment, etc. Existing flow cytometry antibody incubation devices can only incubate one indicator of a sample during one test. If multiple indicators of a sample need to be incubated, multiple repeated operations are required, resulting in low work efficiency.

[0004] Therefore, how to provide a multi-index flow cytometry antibody incubation device to improve work efficiency is an urgent problem to be solved by those skilled in the art. Utility Model Content

[0005] In view of this, the utility model provides a multi-index flow antibody incubation device to solve the problem that the existing incubation device can only incubate one index in a sample during one detection process, resulting in low work efficiency.

[0006] In order to solve the above technical problems, the utility model adopts the following technical solutions:

[0007] A multi-index flow antibody incubation device comprises an incubation tube body, wherein the inner wall of the incubation tube body is rotatably provided with a first partition plate, the first partition plate divides the incubation tube body into a first cavity and a second cavity, and the first partition plate is provided with a plurality of through holes;

[0008] A sample chamber and a plurality of shunt tubes are provided in the first cavity, the sample chamber is provided with a plurality of shunt ports, one end of the shunt tube is connected to the shunt port, and the other end of the shunt tube extends to the first partition plate;

[0009] A plurality of second partitions are disposed in the second cavity, and the second partitions divide the second cavity into a plurality of incubation chambers. By rotating the first partitions, the incubation chambers can be in a connected state or a closed state with the shunt tube.

[0010] Preferably, along the flow direction of the fluid, the cross-sectional area of ​​the sample chamber gradually decreases.

[0011] Preferably, the plurality of diversion ports are evenly distributed at one end of the sample chamber close to the bottom.

[0012] Preferably, the shunt tube is inclined from the middle of the incubation tube body to a side close to the inner wall of the incubation tube body.

[0013] Preferably, the diverter tube is cylindrical.

[0014] Preferably, it also includes an end cover, which is arranged on the top of the incubation tube body.

[0015] Preferably, the incubation tube body and the end cap are both made of light-proof material.

[0016] The utility model provides a multi-index flow antibody incubation device, which has the following beneficial effects compared with the prior art:

[0017] The utility model divides an incubation tube body into a first cavity and a second cavity by rotating a first partition, wherein the sample chamber is located in the first cavity and the incubation chamber is located in the second cavity, and the sample in the sample chamber is evenly diverted to multiple incubation chambers through multiple shunt tubes; at the same time, by rotating the first partition, it is possible to change whether the shunt tube is connected or not connected to the through hole of the first partition; when the shunt tube and the through hole of the first partition are in a non-connected state, multiple incubation chambers are all in a closed state with their corresponding shunt tubes, and samples are stored in the shunt tubes; after the samples in each shunt tube are evenly distributed, the first partition is rotated until the shunt tube and its through hole are in a connected state, at which time multiple incubation chambers are all in a connected state with their corresponding shunt tubes, and the samples smoothly enter each incubation chamber, thereby realizing simultaneous incubation of multiple indicators. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0019] Figure 1 It is a structural schematic diagram of the multi-index flow antibody incubation device of the utility model;

[0020] Figure 2 It is a partial structural schematic diagram of the multi-index flow-type antibody incubation device of the utility model;

[0021] Figure 3 It is a top view of the first partition and the parts below.

[0022] 100 - incubation tube body, 110 - first cavity, 111 - sample chamber, 112 - diversion tube, 113 - diversion port, 120 - second cavity, 121 - incubation chamber, 200 - end cover, 300 - first partition, 310 - through hole, 400 - second partition. DETAILED DESCRIPTION

[0023] The following is a further detailed description of the specific implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0024] In the description of the present application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0025] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, "plurality" means two or more.

[0026] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0027] like Figure 1 As shown, an embodiment of the utility model provides a multi-index flow antibody incubation device, including an incubation tube body 100 and an end cover 200 , wherein the end cover 200 is disposed on the top of the incubation tube body 100 .

[0028] In some embodiments of the present invention, the inner wall of the incubation tube 100 is rotatably provided with a first partition 300, and the first partition 300 divides the incubation tube 100 into a first cavity 110 and a second cavity 120, and Figure 3As shown, the first partition 300 is provided with a plurality of through holes 310; a sample chamber 111 and a plurality of shunt tubes 112 are provided in the first cavity 110, a plurality of shunt ports 113 are provided in the sample chamber 111, one end of the shunt tube 112 is connected to the shunt port 113, and the other end extends to the first partition 300; a plurality of second partitions 400 are provided in the second cavity 120, and the second partitions 400 divide the second cavity 120 into a plurality of incubation chambers 121, and by rotating the first partition 300, the incubation chamber 121 can be in a connected state or a closed state with the shunt tube 112.

[0029] Alternatively, if Figure 2 , Figure 3 As shown, the first partition 300 can be a whole or composed of multiple plates. The first partition 300 is rotatably connected to the inner wall of the incubation tube 100. There is no special limitation on how to achieve the rotatable connection, as long as the shunt tube 112 and the through hole 310 of the first partition 300 can be connected or closed. For example, a protrusion is provided on the inner wall of the incubation tube 100, and a groove matching the protrusion is provided on the outer edge of the first partition 300. Under the condition that the groove and the protrusion match, the first partition 300 can be rotated to change the relative position of the through hole 310 and the end of the shunt tube 112, thereby realizing the connection state or the closed state of the incubation chamber 121 and the shunt tube 112.

[0030] Optionally, the second cavity 120 can be divided into at least two incubation chambers 121 by multiple second partitions 400. For example, the number can be two, three, four, five, etc., which is not particularly limited and can be adjusted according to actual usage; and the number of incubation chambers 121 and the number of shunt tubes 112 correspond to the number of through holes 310 on the first partition 300, forming multiple sample detection units to achieve simultaneous incubation of multiple indicators.

[0031] Optionally, the required antibodies are attached to the interior of the incubation chamber 121, and the material of the incubation chamber 121 is a light-proof material to provide a good environment for the incubation process.

[0032] It can be understood that before injection, the first partition 300 is rotated to a closed state where the shunt tube 112 and the through hole 310 are not connected. The sample enters through the injection port of the sample chamber 111 and is then diverted to multiple shunt tubes 112. After the sample is evenly distributed in each shunt tube 112, the first partition 300 is adjusted to make the shunt tube 112 and the through hole 310 connected. The sample enters each incubation chamber 121 and contacts the antibody on the inner wall of the incubation chamber 121 to start the incubation process.

[0033] In some embodiments of the present invention, along the flow direction of the fluid, the cross-sectional area of ​​the sample chamber 111 gradually decreases, and is preferably an inverted cone or a frustum.

[0034] Optionally, a plurality of diversion ports 113 are evenly distributed at one end of the sample chamber 111 close to the bottom.

[0035] It should be noted that the end close to the bottom here does not refer to the absolute bottom or the side wall close to the bottom, but needs to be adapted to the specific shape of the sample chamber 111 so that the sample can be better and faster gathered and enter the diverter tube 112. For example, when the sample chamber 111 is an inverted cone, the diverter ports 113 are evenly distributed on the side wall close to the bottom of the sample chamber 111; and when the sample chamber 111 is an inverted frustum, the diverter ports 113 can be evenly distributed at the bottom of the sample chamber 111.

[0036] In some embodiments of the present invention, the shunt tube 112 is inclined from the middle of the incubation tube body 100 to a side close to the inner wall of the incubation tube body 100 . Preferably, the shunt tube 112 is cylindrical.

[0037] It should be noted that the utility model does not impose any special limitation on the inclination angle of the shunt tube 112 , which can be determined according to the space size of the first cavity 110 and the number of the shunt ports 113 , and the shunt tube 112 is set as a cylinder to make the sample flow more smoothly.

[0038] The specific use method of the utility model is described below in conjunction with the incubation process:

[0039] 1. Preparation of Cell Suspension

[0040] (1) Collect the cell culture medium rich in cells into a centrifuge tube and centrifuge at room temperature at 2000 rpm for 5 min;

[0041] (2) After centrifugation, discard the supernatant, resuspend the cells in DPBS, and centrifuge at room temperature at 2000 rpm for 5 min;

[0042] (3) After centrifugation, discard the supernatant and resuspend the cells in DPBS to a cell density of 1*10 6 cel ls / ml.

[0043] 2. Sample addition

[0044] (1) adjusting the first partition so that the sample shunt tube is not connected to the antibody incubation chamber;

[0045] (2) Add 800 μL of cell suspension from the sample inlet of the sample chamber, and evenly distribute the cell suspension to each shunt tube through the shunt port. Let it stand to allow the sample to be evenly distributed in the shunt tube;

[0046] (3) Adjust the first partition so that the sample shunt tube is connected to the antibody incubation chamber, and the sample enters the antibody incubation chamber through the through hole.

[0047] 3. Incubation

[0048] (1) The sample entering the antibody incubation chamber comes into contact with the antibody and is shaken and mixed;

[0049] (2) Place the antibody incubation chamber at room temperature and incubate in the dark for 15 minutes.

[0050] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and substitutions can be made without departing from the technical principle of the present invention. These improvements and substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A multi-index flow cytometry antibody incubation device, characterized in that: It comprises an incubation tube body, the inner wall of which is rotatably provided with a first partition, the first partition divides the incubation tube body into a first cavity and a second cavity, and the first partition is provided with a plurality of through holes; A sample chamber and a plurality of shunt tubes are provided in the first cavity, the sample chamber is provided with a plurality of shunt ports, one end of the shunt tube is connected to the shunt port, and the other end of the shunt tube extends to the first partition plate; A plurality of second partitions are disposed in the second cavity, and the second partitions divide the second cavity into a plurality of incubation chambers. By rotating the first partitions, the incubation chambers can be in a connected state or a closed state with the shunt tube.

2. The multi-index flow cytometry antibody incubation device according to claim 1, characterized in that: Along the flow direction of the fluid, the cross-sectional area of ​​the sample chamber gradually decreases.

3. The multi-index flow cytometry antibody incubation device according to claim 1, characterized in that: The plurality of diversion ports are evenly distributed at one end of the sample chamber close to the bottom.

4. The multi-index flow cytometry antibody incubation device according to claim 1, characterized in that: The shunt pipe is arranged obliquely from the middle part of the incubation tube body to one side close to the inner wall of the incubation tube body.

5. The multi-index flow cytometry antibody incubation device according to claim 1, characterized in that: The shunt pipe is cylindrical.

6. The multi-index flow cytometry antibody incubation device according to any one of claims 1 to 5, characterized in that: The incubation chamber is made of light-proof material.

7. The multi-index flow cytometry antibody incubation device according to claim 6, characterized in that: It also includes an end cover, which is arranged on the top of the incubation tube body.

Images