Device for visualizing oligonucleotide solid-phase synthesis column
By setting up a visible window in the cylinder of the solid-phase synthesis column, real-time observation of the reaction progress is achieved, and the problem that traditional synthesis columns cannot monitor the reaction conditions is solved, and the synthesis efficiency and product quality are improved.
Patent Information
- Application Number
- CN202421874067.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-05
- Publication Date
- 2025-05-16
- Estimated Expiration
- 2034-08-05
AI Technical Summary
Traditional solid-phase synthesis columns cannot monitor the progress of the reaction in real time, resulting in unstable quality of the synthetic product.
A device for visualizing a solid-phase synthesis column of oligonucleotides is designed, and real-time observation of reaction progress is achieved by setting a first visual window on the outer and inner side of the cylinder, and forming a second visual window through two sets of first visual windows.
By monitoring the progress of the reaction in real time, the synthesis conditions can be adjusted in a timely manner to improve the synthesis efficiency and product quality.
Smart Images
Figure CN222872148U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of organic synthesis, in particular to a device for visualizing an oligonucleotide solid phase synthesis column. Background Art
[0002] Oligonucleotide synthesis is a common operation in biotechnology, which is used to synthesize DNA and RNA with specific sequences. In traditional solid-phase synthesis, the reaction process in the medium-scale stainless steel synthesis column is usually invisible, and the operator cannot monitor the progress of the reaction in real time, which may lead to unstable quality of the synthesized product. Therefore, the development of a visual monitoring synthesis device is crucial to improve the synthesis efficiency and product quality.
[0003] In the biological industry, the synthesis of oligonucleotides usually adopts the solid phase synthesis method, which is a kind of chemical synthesis.
[0004] Oligonucleotide synthesis usually includes four steps: deprotection, coupling, oxidation / sulfurization and capping.
[0005] The solid phase carrier will continue to expand during the synthesis process. The traditional stainless steel synthesis column cannot monitor the reaction conditions in the column and cannot adjust the column height accordingly.
[0006] In view of this, we propose a device for visualizing oligonucleotide solid phase synthesis columns. Utility Model Content
[0007] In order to solve the problem that the existing device cannot monitor the reaction status in the column, the utility model provides a device for visualizing the oligonucleotide solid phase synthesis column.
[0008] The utility model is achieved in this way:
[0009] A device for visualizing an oligonucleotide solid phase synthesis column comprises a cylinder, wherein the cylinder comprises an outer side of the cylinder and an inner side of the cylinder, a first visual window is provided at the connection between the outer side of the cylinder and the inner side of the cylinder, two groups of the first visual windows are interconnected and constitute a second visual window, an upper support plate is connected to the top of the cylinder, a hydraulic port is provided at the top of the upper support plate, the bottom end of the cylinder is connected to a lower support plate, an adapter is provided between the upper support plate and the lower support plate, the adapter is located inside the cylinder, and scale lines are provided on the outer wall of the adapter.
[0010] Furthermore, a screw is threadedly connected to the top end of the upper support plate, and the upper support plate is connected to a fixer via the screw.
[0011] The beneficial effect of adopting the above further solution is that the upper support plate and the fixer are easily fixed through the connection of the screw rod.
[0012] Furthermore, the top of the adapter extends to the top of the fixture and is provided with a synthesis column inlet, one end of which is provided with a TC25 connector.
[0013] Furthermore, the bottom end of the adapter extends to the bottom of the lower support plate and is provided with a synthesis column outlet.
[0014] Furthermore, a lower sieve plate is installed at the bottom end of the cylinder.
[0015] The beneficial effect of adopting the above further solution is that when the adapter contacts the lower screen plate, the screen rod exposed on the upper support plate is at zero scale and moves downward in sequence.
[0016] Furthermore, an upper sieve plate is installed at the bottom end of the adapter, and a sealing ring is provided at the contact portion between the upper sieve plate and the cylinder.
[0017] The beneficial effect of adopting the above further solution is that the sealing of the entire synthesis column is ensured by the provision of the sealing ring.
[0018] Furthermore, the sealing ring is made of FFKM material.
[0019] Furthermore, the material of the inner side of the cylinder is borosilicate glass, and the material of the outer side of the cylinder is 316L stainless steel.
[0020] Furthermore, a bracket is installed at the bottom end of the lower support plate.
[0021] The beneficial effect of adopting the above further solution is that the stability of the device can be easily supported by installing and using the bracket.
[0022] The beneficial effect of the utility model is that the reaction progress inside the device can be observed in real time by using the outer side of the cylinder, the inner side of the cylinder, the first visual window and the second visual window in coordination, wherein a double-layer structure is formed by the cylinder, and the first visual window is arranged in the double-layer structure, and the positional relationship of the two groups of first visual windows is coordinated, so that the scale line on the adapter can be directly observed through the constructed second visual window, thereby realizing the visualization function of the device. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] In order to more clearly illustrate the technical solution of the implementation mode of the utility model, the drawings required for use in the implementation mode will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.
[0024] Figure 1This is an overall front view of a device for visualizing an oligonucleotide solid phase synthesis column provided by the utility model;
[0025] Figure 2 A front view of an adapter structure of a device for visualizing an oligonucleotide solid phase synthesis column provided by the utility model;
[0026] Figure 3 The utility model provides a front view of the barrel structure of a device for visualizing an oligonucleotide solid phase synthesis column.
[0027] In the figure: 1. Fixer; 2. Upper support plate; 3. Synthesis column inlet; 4. Cylinder; 5. Lower support plate; 6. Synthesis column outlet; 7. Bracket; 8. Adapter; 9. Lower sieve plate; 10. First visual window; 11. Screw; 12. Hydraulic port; 13. TC25 connector; 14. Scale line; 15. Upper sieve plate; 16. Second visual window; 17. Outside of cylinder; 18. Inside of cylinder. DETAILED DESCRIPTION
[0028] In order to make the purpose, technical solution and advantages of the implementation of the utility model clearer, the technical solution in the implementation of the utility model will be clearly and completely described below in conjunction with the drawings in the implementation of the utility model. Obviously, the described implementation is a part of the implementation of the utility model, not all of the implementations. Based on the implementation of the utility model, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0029] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention.
[0030] Embodiment 1
[0031] See also Figure 1-Figure 3 The utility model provides a technical solution: a device for visualizing an oligonucleotide solid phase synthesis column, comprising a cylinder 4, the cylinder 4 comprising an outer cylinder 17 and an inner cylinder 18, a first visual window 10 is provided at the connection between the outer cylinder 17 and the inner cylinder 18, two groups of first visual windows 10 are interconnected and constitute a second visual window 16, an upper support plate 2 is connected to the top of the cylinder 4, and a hydraulic port 12 is provided at the top of the upper support plate 2, the bottom end of the cylinder 4 is connected to a lower support plate 5, an adapter 8 is provided between the upper support plate 2 and the lower support plate 5, and the adapter 8 is located inside the cylinder 4, and a scale line 14 is provided on the outer wall of the adapter 8.
[0032] Embodiment 2
[0033] See also Figure 1-Figure 3 As an embodiment of the utility model, further, the top end of the upper support plate 2 is threadedly connected with a screw rod 11, and the upper support plate 2 is connected to the fixture 1 through the screw rod 11. The connection of the screw rod 11 facilitates the fixation between the upper support plate 2 and the fixture 1. The top end of the adapter 8 extends to the top of the fixture 1 and is provided with a synthetic column inlet 3. One end of the synthetic column inlet 3 is provided with a TC25 connector 13. The bottom end of the adapter 8 extends to the bottom of the lower support plate 5 and is provided with a synthetic column outlet 6.
[0034] Embodiment 3
[0035] See also Figure 1-Figure 3 As an embodiment of the utility model, further, a lower sieve plate 9 is installed at the bottom end of the cylinder 4. When the adapter 8 contacts the lower sieve plate 9, the sieve rod exposed on the upper support plate 2 is at zero scale, and then downward, an upper sieve plate 15 is installed at the bottom end of the adapter 8. The contact portion between the upper sieve plate 15 and the cylinder 4 is provided with a sealing ring. The sealing of the entire synthetic column is ensured by the setting of the sealing ring. The sealing ring is made of FFKM material, the material of the inner side 18 of the cylinder is borosilicate glass, and the material of the outer side 17 of the cylinder is 316L stainless steel. A bracket 7 is installed at the bottom end of the lower support plate 5. The installation and use of the bracket 7 facilitates the support of the stability of the device.
[0036] Specifically, the working principle of the device of the visualized oligonucleotide solid phase synthesis column is as follows: when in use, the staff first unscrews the screw 11, takes out the fixture 1, the upper support plate 2 and the adapter 8, blocks the column outlet 6 with a TC blind plate, pours the solid phase carrier into the column cavity, adds anhydrous acetonitrile into the column cavity, then puts the adapter 8 into the column cavity, covers the upper support plate, and fixes it with a nut. Block one side of the hydraulic port 13 with a TC25 blind plate, connect a pipeline with the synthetic column inlet 3 and the TC25 connector, pump liquid to the other side of the hydraulic port, press the adapter 3 down to a suitable position by hydraulic means, and then fix the fixture 1 with the screw 11. When disassembling the column, first remove the screw 11 and the fixture 1, block the synthetic column outlet 6 and one side of the hydraulic port 12, pump liquid through the synthetic column inlet 3, and lift the adapter 8. During the production process, the staff can observe the expansion of the solid phase carrier in the column in real time through the visual window 16.
[0037] The above description is only the preferred implementation of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.
Claims
1. A device for visualizing an oligonucleotide solid phase synthesis column, comprising a cylinder (4), characterized in that: The cylinder (4) comprises an outer side (17) and an inner side (18) of the cylinder. A first visible window (10) is provided at the connection between the outer side (17) and the inner side (18) of the cylinder. Two groups of the first visible windows (10) are interconnected to form a second visible window (16). The top end of the cylinder (4) is connected to an upper support plate (2), and a hydraulic port (12) is provided at the top end of the upper support plate (2). The bottom end of the cylinder (4) is connected to a lower support plate (5). An adapter (8) is provided between the upper support plate (2) and the lower support plate (5), and the adapter (8) is located inside the cylinder (4). The outer wall of the adapter (8) is provided with a scale mark (14).
2. The device for visualizing oligonucleotide solid phase synthesis column according to claim 1, characterized in that: The top end of the upper support plate (2) is threadedly connected to a screw rod (11), and the upper support plate (2) is connected to a fixer (1) via the screw rod (11).
3. The device for visualizing oligonucleotide solid phase synthesis column according to claim 2, characterized in that: The top end of the adapter (8) extends to the top of the fixture (1) and is provided with a synthesis column inlet (3), one end of which is sleeved with a TC25 connector (13).
4. The device for visualizing oligonucleotide solid phase synthesis column according to claim 3, characterized in that: The bottom end of the adapter (8) extends to the bottom of the lower support plate (5) and is provided with a synthesis column outlet (6).
5. The device for visualizing oligonucleotide solid phase synthesis column according to claim 1, characterized in that: A lower sieve plate (9) is installed at the bottom end of the cylinder (4).
6. The device for visualizing oligonucleotide solid phase synthesis column according to claim 1, characterized in that: An upper sieve plate (15) is installed at the bottom end of the adapter (8), and a sealing ring is provided at the contact portion between the upper sieve plate (15) and the cylinder (4).
7. The device for visualizing oligonucleotide solid phase synthesis column according to claim 6, characterized in that: The sealing ring is made of FFKM material.
8. The device for visualizing oligonucleotide solid phase synthesis column according to claim 1, characterized in that: The material of the inner side (18) of the cylinder is borosilicate glass, and the material of the outer side (17) of the cylinder is 316L stainless steel.
9. The device for visualizing oligonucleotide solid phase synthesis column according to claim 1, characterized in that: A bracket (7) is installed at the bottom end of the lower support plate (5).