Nerve cell culture device
By designing a nerve cell culture device with current regulation components, the problem of lack of electrical stimulation function and current inability to regulate in the prior art is solved, and fine current control of the nerve cell culture area is achieved, which improves the convenience of experiments and the accuracy of data.
Patent Information
- Application Number
- CN202421755295.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-24
- Publication Date
- 2025-05-16
- Estimated Expiration
- 2034-07-24
AI Technical Summary
The existing in vitro cell culture devices do not have the function of electrical stimulation, which makes it difficult for nerve cells to achieve electrical stimulation during growth and development, and the current size is fixed and cannot be adjusted, which affects the accuracy and cost of the experiment.
A neural cell culture device was designed, including a culture bottle assembly and current regulation assembly. The culture bottle assembly divides the inner space of the bottle into two areas through a partition plate. Each area is equipped with a conductive sheet, a slide groove, a resistive wire, a connecting rod and a sliding cover. The resistance of the resistive wire is changed by sliding the sliding cover to adjust the current size.
Independent current control and regulation of nerve cell culture areas is achieved, which improves the convenience of experiments and the accuracy of data, while reducing research costs.
Smart Images
Figure CN222877963U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a nerve cell culture device. Background Art
[0002] Most of the existing in vitro cell cultures are carried out in culture bottles. At the same time, through research and development, it has been discovered that human nerve cells have electrical activity during development, but the existing culture bottles do not have electrical stimulation devices. Therefore, when nerve cells need to be cultured, temporary electrodes need to be set up in the culture bottles to provide electrical stimulation during the growth and development of nerve cells.
[0003] However, setting up temporary electrodes in culture bottles is time-consuming and laborious, and affects the sealing of the culture bottles. At the same time, when conducting experiments inside existing culture bottles, the current size is fixed and cannot be adjusted. Multiple culture bottles and different current equipment are required to conduct experiments, which greatly increases the cost. In addition, the nerve cells in different culture bottles are different due to different environments, which affects the accuracy of the data.
[0004] Therefore, it is necessary to invent a neural cell culture device to solve the above problems. Utility Model Content
[0005] The purpose of the utility model is to provide a neural cell culture device to solve the problems raised in the above background technology.
[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a neural cell culture device, comprising a culture bottle assembly, wherein a current regulating component is installed on the top of the culture bottle assembly;
[0007] The culture bottle assembly comprises a bottle body, one side of the bottle body is integrally formed with a bottle mouth, a partition plate is integrally formed in the middle of the bottle body, and one end of the partition plate is located in the middle of an opening at one end of the bottle mouth;
[0008] The current regulating component comprises two conductive sheets, both of which are fixedly arranged on the inner wall of the bottom end of the bottle body, and the two conductive sheets are respectively located on both sides of the partition plate.
[0009] Preferably, the bottle mouth is arranged to be tilted upward, a bottle cap is installed at one end of the bottle mouth away from the bottle body, and the front and back sides of the bottle body are integrally formed with a plurality of scale lines distributed at equal intervals.
[0010] Preferably, the top and bottom edges of the bottle body are integrally formed with a skirt, a first protective cover and a second protective cover are fixedly provided on both sides of the top of the bottle body respectively, and the front and rear ends of the inner wall of the bottom end of the bottle body close to the bottle mouth are integrally formed with a slope.
[0011] Preferably, a third protective cover is fixedly provided at the front and rear ends of the top of the bottle body, a slide groove is penetrated through the top of the third protective cover, a resistance wire is fixedly provided inside the third protective cover, and one end of the resistance wire penetrates through the inner wall of one end of the third protective cover.
[0012] Preferably, the top two ends of the conductive sheet are electrically connected with a first conductive wire and a second conductive wire respectively, and the other end of the second conductive wire is fixedly passed through the top inner wall of the bottle body and electrically connected to one end of the resistance wire, and the top of the first conductive wire is fixedly passed through the top inner wall of the bottle body.
[0013] Preferably, a connecting rod is provided through the interior of the slide groove, and the bottom end of the connecting rod is integrally formed with an arc-shaped head adapted to the resistance wire, a sliding cover is fixedly provided on the top end of the connecting rod, and an opening is provided on one side of the sliding cover, the bottom end of the sliding cover is fit-connected with the top end of the third protective cover, and a connecting wire is electrically connected on the connecting rod.
[0014] Preferably, limiting strips matching the arc-shaped head are fixedly provided on the top of the inner walls on both sides of the third protective cover, and the two ends of the outer wall of the arc-shaped head are respectively fitted and connected with the inner bottom ends of the limiting strips.
[0015] Preferably, a first electrode connector and a second electrode connector are respectively mounted on the first protective cover and the second protective cover, and the second electrode connector is electrically connected to one end of two connecting wires, and the first electrode connector is electrically connected to the top ends of two first conductive wires.
[0016] Technical effects and advantages of the utility model:
[0017] The utility model divides the internal space of the bottle body into two areas by a partition plate, and conductive sheets are arranged inside the two areas at the same time, and independent slide grooves, resistance wires, connecting rods and sliding covers are arranged in the two areas. When a uniform voltage is used, the sliding cover is slid to drive the arc head to slide on the resistance wire, so that the distance that the current passes through the resistance wire changes, thereby changing the resistance of the resistance wire in the circuit system. The change in resistance can change the current size, so that the current size of the two areas can be independently controlled and adjusted at any time. The device not only improves the convenience of the experiment and greatly reduces the research cost, but also improves the accuracy of the experimental data when the experiment is carried out in the same environment, and has strong practicality. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the utility model.
[0019] Figure 2 This is a schematic diagram of the culture bottle assembly structure of the utility model.
[0020] Figure 3This is a schematic diagram of the structure of the current regulating component of the utility model.
[0021] Figure 4 It is a schematic diagram of the cross-sectional structure of the third protective cover of the present utility model.
[0022] Figure 5 This is a schematic diagram of the circuit principle structure of the utility model.
[0023] In the figure: 1. culture bottle assembly; 2. current regulating component; 101. bottle body; 102. bottle mouth; 103. bottle cap; 104. skirt; 105. first protective cover; 106. second protective cover; 107. scale line; 108. partition plate; 109. slope; 201. conductive sheet; 202. third protective cover; 203. first conductive wire; 204. first electrode connector; 205. second conductive wire; 206. second electrode connector; 207. connecting wire; 208. slide groove; 209. connecting rod; 210. arc head; 211. sliding cover; 212. resistance wire; 213. limit strip. DETAILED DESCRIPTION
[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0025] The utility model provides Figure 1-5 A neural cell culture device shown includes a culture bottle assembly 1 , and a current regulating component 2 is installed on the top of the culture bottle assembly 1 .
[0026] Furthermore, the culture bottle assembly 1 includes a bottle body 101, one side of the bottle body 101 is integrally provided with a bottle mouth 102, the middle part of the bottle body 101 is integrally provided with a partition plate 108, and one end of the partition plate 108 is located in the middle of the opening of one end of the bottle mouth 102, the bottle mouth 102 is inclined upward, and the inclined setting is conducive to convenient pipetting and reducing the blind spot of pipetting. A bottle cap 103 is installed at one end of the bottle mouth 102 away from the bottle body 101, and the bottle cap 103 adopts a 0.22um ventilation, water-blocking and high-efficiency bacteria-blocking filter membrane type. The front and back sides of the bottle body 101 are integrally provided with multiple scale lines 107 distributed at equal intervals, and a first protective cover 105 and a second protective cover 106 are fixedly provided on both sides of the top of the bottle body 101.
[0027] The current regulating component 2 includes two conductive sheets 201, which are fixedly arranged on the inner wall of the bottom end of the bottle body 101, and the two conductive sheets 201 are respectively located on both sides of the partition plate 108. The top front end and the rear end of the bottle body 101 are fixedly arranged with a third protective cover 202, and the top of the third protective cover 202 is penetrated with a slide groove 208. The inside of the third protective cover 202 is fixedly arranged with a resistance wire 212, and one end of the resistance wire 212 penetrates the inner wall of one end of the third protective cover 202. The resistance wire 212 is made of nickel-chromium metal with large resistance. The two ends of the top of the conductive sheet 201 are respectively electrically A first conductive wire 203 and a second conductive wire 205 are connected, and the other end of the second conductive wire 205 is fixedly passed through the top inner wall of the bottle body 101 and is electrically connected to one end of the resistance wire 212. The top of the first conductive wire 203 is fixedly passed through the top inner wall of the bottle body 101, and a connecting rod 209 is movably passed through the inner part of the slide groove 208, and the bottom end of the connecting rod 209 is integrally formed with an arc head 210 adapted to the resistance wire 212, and a sliding cover 211 is fixedly provided on the top of the connecting rod 209, and one side of the sliding cover 211 is opened, and the bottom end of the sliding cover 211 is connected to the first conductive wire 203. The tops of the three protective covers 202 are fitted and connected, and a connecting wire 207 is electrically connected to the connecting rod 209. The first protective cover 105 and the second protective cover 106 are respectively installed with a first electrode connector 204 and a second electrode connector 206, and the second electrode connector 206 is electrically connected to one end of the two connecting wires 207, and the first electrode connector 204 is electrically connected to the tops of the two first conductive wires 203. The internal space of the bottle body 101 is divided into two areas by a partition plate 108, and conductive sheets 201 are arranged inside the two areas, and the two areas are provided with independent The independent slide groove 208, resistance wire 212, connecting rod 209 and sliding cover 211, when using a uniform voltage, the sliding cover 211 is used to drive the arc head 210 to slide on the resistance wire 212, so that the distance that the current passes through the resistance wire 212 changes, thereby changing the resistance of the resistance wire 212 in the circuit system. The change in resistance can change the current size, so that the current size in the two areas can be independently controlled and adjusted at any time. The device not only improves the convenience of the experiment and greatly reduces the research cost, but also improves the accuracy of the experimental data when experiments are carried out in the same environment, and has strong practicality.
[0028] Secondly, the top and bottom edges of the bottle body 101 are integrally formed with a skirt 104. The setting of the skirt 104 is conducive to convenient reinforcement and stacking. The front and rear ends of the inner wall of the bottom end of the bottle body 101 close to the bottle mouth 102 are integrally formed with a slope 109. The top ends of the inner walls on both sides of the third protective cover 202 are fixedly provided with limiting strips 213 that are compatible with the arc head 210, and the two ends of the outer wall of the arc head 210 are respectively fitted and connected with the inner bottom end of the limiting strip 213. The setting of the limiting strip 213 can limit the arc head 210 to prevent the arc head 210 from shaking and detaching from the resistance wire 212, which will affect the experimental effect.
[0029] Note: The number of the partition plates 108 inside the bottle body 101 is not limited to one, and can also be set to multiple, so as to divide the internal space of the bottle body 101 into multiple areas for experiments. Generally, one or two partition plates can be set, such as Figure 5 As shown, there are several branches of the resistor wires 212 and the conductive sheet 201 connected in parallel in several regions.
[0030] Working principle of this utility model:
[0031] When in use, the two ends of the test voltage are connected to the first electrode connector 204 and the second electrode connector 206 respectively, so as to form Figure 5 In the circuit shown, the voltages at both ends of the loop formed by the two resistors 212 and the corresponding conductive sheet 201 are the same, and only part of the resistor 212 is connected to the circuit, and no current flows through the other part. During the experiment, the sliding cover 211 can be slid to drive the connecting rod 209 and the arc head 210 to slide, and the position of the arc head 210 changes, thereby changing the length of the current passing through the resistor 212, thereby changing the resistance of the resistor 212 connected to the circuit. The resistance changes, and thus the loop current of the resistor 212 and the corresponding conductive sheet 201 changes. The greater the resistance, the smaller the current, and vice versa. The greater the current, the greater the current. The current passing through the conductive sheet 201 changes, so that the current of the two areas can be controlled separately, and the partition management experiment can be carried out.
[0032] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A neural cell culture device, characterized in that: It comprises a culture bottle assembly (1), wherein a current regulating component (2) is installed on the top of the culture bottle assembly (1); The culture bottle assembly (1) comprises a bottle body (101), one side of the bottle body (101) is integrally formed with a bottle mouth (102), a partition plate (108) is integrally formed in the middle of the bottle body (101), and one end of the partition plate (108) is located in the middle of an opening at one end of the bottle mouth (102); The current regulating component (2) comprises two conductive sheets (201), the two conductive sheets (201) are fixedly arranged on the inner wall of the bottom end of the bottle body (101), and the two conductive sheets (201) are respectively located on both sides of the partition plate (108).
2. A neural cell culture device according to claim 1, characterized in that: The bottle mouth (102) is arranged to be inclined upward, and a bottle cap (103) is installed at one end of the bottle mouth (102) away from the bottle body (101), and the front and back sides of the bottle body (101) are integrally formed with a plurality of scale lines (107) distributed at equal intervals.
3. A neural cell culture device according to claim 2, characterized in that: The top and bottom edges of the bottle body (101) are both integrally formed with a skirt (104), the top sides of the bottle body (101) are respectively fixedly provided with a first protective cover (105) and a second protective cover (106), and the bottom inner wall of the bottle body (101) is integrally formed with a slope (109) at the front and rear ends of the side close to the bottle mouth (102).
4. A neural cell culture device according to claim 3, characterized in that: A third protective cover (202) is fixedly provided at the front end and the rear end of the top of the bottle body (101); a slide groove (208) is provided through the top of the third protective cover (202); a resistance wire (212) is fixedly provided inside the third protective cover (202), and one end of the resistance wire (212) passes through the inner wall of one end of the third protective cover (202).
5. A neural cell culture device according to claim 4, characterized in that: The two ends of the top of the conductive sheet (201) are respectively electrically connected with a first conductive wire (203) and a second conductive wire (205), and the other end of the second conductive wire (205) is fixedly passed through the inner wall of the top of the bottle body (101) and is electrically connected to one end of the resistance wire (212), and the top of the first conductive wire (203) is fixedly passed through the inner wall of the top of the bottle body (101).
6. A neural cell culture device according to claim 5, characterized in that: A connecting rod (209) is provided inside the slide groove (208) so as to be movable and penetrated, and the bottom end of the connecting rod (209) is integrally formed with an arc-shaped head (210) adapted to the resistance wire (212), a sliding cover (211) is fixedly provided at the top end of the connecting rod (209), and one side of the sliding cover (211) is provided with an opening, the bottom end of the sliding cover (211) is fitted and connected with the top end of the third protective cover (202), and a connecting wire (207) is electrically connected to the connecting rod (209).
7. A neural cell culture device according to claim 6, characterized in that: Limiting strips (213) adapted to the arc-shaped head (210) are fixedly provided at the top ends of the inner walls on both sides of the third protective cover (202), and the two ends of the outer wall of the arc-shaped head (210) are respectively fitted and connected to the inner bottom ends of the limiting strips (213).
8. A neural cell culture device according to claim 7, characterized in that: The first protective cover (105) and the second protective cover (106) are respectively mounted with a first electrode connector (204) and a second electrode connector (206), and the second electrode connector (206) is electrically connected to one end of two connecting wires (207), and the first electrode connector (204) is electrically connected to the top ends of the two first conductive wires (203).