Secondary electrospray ionization source gas path system and secondary electrospray ionization source

Abstract

The utility model discloses a secondary electrospray ionization source gas path system and a secondary electrospray ionization source. Comprising a buffer assembly used for receiving input sheath gas and reducing turbulent flow of the sheath gas, a first pressure regulating assembly used for reducing the gas pressure of the sheath gas for the first time, a switch assembly used for controlling connection or disconnection of a sheath gas pipeline, and a second pressure regulating assembly used for reducing the gas pressure of the sheath gas for the second time, the electrospray bottle assembly is used for evaporating liquid to form ions and conveying the ions into a mass spectrometer; in conclusion, by arranging the secondary electrospray ionization source gas path system, the device can be automatically controlled through the control assembly to adjust the sheath gas pressure, a knob does not need to be manually controlled to adjust the sheath gas pressure in a pipeline, and a plurality of knobs do not need to be arranged on the secondary electrospray ionization source. And the miniaturization and integration design of the equipment is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of mass spectrometry equipment, in particular to a secondary electrospray ionization source gas path system and a secondary electrospray ionization source. Background Art

[0002] Secondary electrospray ionization (SESI) is a modern ionization mass spectrometry technology developed on the basis of electrospray ionization (ESI) and electrospray desorption ionization (DESI). SESI is a commonly used mass spectrometry analysis technology that can convert sample molecules into charged ions and then analyze them through a mass spectrometer. The working principle of the secondary electrospray ionization source is to spray the sample solution into fine droplets through a high-voltage electric field, and then under the action of the electric field, the droplets gradually evaporate to form charged molecular ions. These ions will be introduced into the mass spectrometer for analysis. Compared with the traditional electrospray ionization source, the secondary electrospray ionization source has a higher ion yield and better stability. The application range of the secondary electrospray ionization source is very wide. In the biomedical field, it can be used for the analysis of biological macromolecules such as proteins, swellings, and nucleic acids. In the field of environmental monitoring, it can be used for the analysis of samples such as water, soil, and air. In the field of food safety, it can be used to detect harmful substances such as additives, pesticides, and heavy metals in food. Secondary electrospray ionization source is a very important mass spectrometry analysis technology, which has broad application prospects in the fields of biomedicine, environmental monitoring, food safety, etc. This technology has the advantages of high sensitivity, high resolution, high throughput, etc. With the continuous development and improvement of the technology, it will play an important role in more fields.

[0003] The premise for the stable operation of secondary electrospray ionization is that there is a stable sheath gas (0.13Mpa) pressure entering the electrospray bottle, and the electrospray solution is transported from the capillary to the capillary atomization port of the ionization source chamber under the action of the +2.5kV electrospray solution charge to form a nanoliter electrospray. Stable sheath gas pressure regulation helps control the electrospray flow entering the mass spectrometer.

[0004] The Chinese patent application (application number: CN202410289625.8) proposes a sample injection heating device for a secondary electrospray ionization source device, such as Figure 1 As shown, the delivery of sheath gas in the device is controlled by a manual mechanical valve. The adjustment steps are relatively complicated, and the knob of the device needs to be placed on the surface of the device, which occupies a large space of the device and is not conducive to the miniaturization improvement of the device. Utility Model Content

[0005] The utility model aims to disclose a secondary electrospray ionization source gas path system, which solves the problem that the flow rate and pressure of the gas path need to be manually controlled in the existing secondary electrospray ionization source gas path system, which occupies a large amount of space in the equipment and is not conducive to the miniaturization improvement of the equipment.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A secondary electrospray ionization source gas path system comprises: a buffer component for receiving input sheath gas and reducing the turbulence of the sheath gas, a first pressure regulating component for reducing the gas pressure of the sheath gas for the first time, a switch component for controlling the connection or disconnection of the sheath gas pipeline, a second pressure regulating component for reducing the gas pressure of the sheath gas for the second time, an electrospray bottle component for evaporating liquid to form ions and transporting them to a mass spectrometer, and a control component; the output end of the buffer component is connected to the input end of the first pressure regulating component; the output end of the first pressure regulating component is connected to the input end of the switch component; the output end of the switch component is connected to the input end of the second pressure regulating component; the output end of the second pressure regulating component is connected to the input end of the electrospray bottle component; the switch component, the second pressure regulating component and the electrospray bottle component are all connected to the control component for communication.

[0008] In one embodiment, the buffer assembly specifically includes: a buffer chamber, the input end of the buffer chamber is connected to the sheath gas source through a hose; the output end of the buffer chamber is connected to the input end of the first pressure regulating assembly.

[0009] In one embodiment, the first pressure regulating component specifically includes: a manual pressure regulating valve; an input end of the manual pressure regulating valve is connected to the buffer chamber; and an output end of the manual pressure regulating valve is connected to an input end of the switch component.

[0010] In one embodiment, the switch component specifically includes: a solenoid valve; the input end of the solenoid valve is connected to the output end of the manual pressure regulating valve; the output end of the solenoid valve is connected to the input end of the second pressure regulating component; and the signal receiving end of the solenoid valve is communicatively connected to the control component.

[0011] In one embodiment, the second pressure regulating component specifically includes: a proportional valve; the input end of the proportional valve is connected to the output end of the solenoid valve; the output end of the proportional valve is connected to the electric spray bottle assembly; and the signal receiving end of the proportional valve is communicatively connected to the control assembly.

[0012] In one embodiment, the electrospray bottle assembly includes: an electrospray bottle and a capillary; the input end of the electrospray bottle is connected to the output end of the proportional valve; the output end of the electrospray bottle is connected to one end of the capillary; and the other end of the capillary is connected to a mass spectrometer.

[0013] A secondary electrospray ionization source comprises: a housing, a display module, a sample gas transmission tube, an ionization chamber and the secondary electrospray ionization source gas path system; the display module, the ionization chamber and the secondary electrospray ionization source gas path system are all accommodated inside the housing; the display module is communicatively connected to the control component; one end of the sample gas transmission tube is connected to the first input end of the ionization chamber; the output end of the secondary electrospray ionization source gas path system is connected to the second input end of the ionization chamber; and the output end of the ionization chamber is connected to a mass spectrometer.

[0014] In one embodiment, the interior of the housing is provided with: a fixing plate; the buffer chamber, the manual pressure regulating valve, the solenoid valve, the electrospray bottle module and the proportional valve are all fixedly connected to the fixing plate.

[0015] In one embodiment, the shell includes a first side panel and a second side panel, which are fixedly connected and arranged perpendicular to each other; the display module is fixedly connected to the first side panel; and the handle of the manual pressure regulating valve extends to the outside of the shell after passing through the second side panel.

[0016] In one embodiment, the shell also includes a third side plate; the third side plate and the first side plate are fixedly connected and arranged perpendicular to each other; a fixing flange for fixing the sample gas transmission tube is fixedly arranged on the third side plate, and the fixing flange is fixedly connected to the third side plate; the sample gas transmission tube extends to the outside of the shell after passing through the through hole on the fixing flange.

[0017] Compared with the prior art, the utility model has the following beneficial effects:

[0018] The utility model provides a secondary electrospray ionization source gas path system and a secondary electrospray ionization source, comprising: a buffer component for receiving input sheath gas and reducing the turbulence of the sheath gas, a first pressure regulating component for reducing the gas pressure of the sheath gas for the first time, a switch component for controlling the connection or disconnection of the sheath gas pipeline, a second pressure regulating component for reducing the sheath gas pressure for the second time, an electrospray bottle component for evaporating liquid to form ions and transporting them to a mass spectrometer, and a control component; in summary, the utility model, by setting a secondary electrospray ionization source gas path system, can automatically control the device to adjust the pressure of the sheath gas through the control component, and there is no need to manually control the knob to adjust the sheath gas pressure in the pipeline, nor is there a need to set multiple knobs on the secondary electrospray ionization source, which is conducive to the miniaturization and integrated design of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0020] Figure 1 It is a three-dimensional schematic diagram of a secondary electrospray ionization source in the prior art;

[0021] Figure 2 This is a schematic diagram of the pipeline connection of a secondary electrospray ionization source gas path system of the present application;

[0022] Figure 3 It is a stereoscopic schematic diagram of a gas path system of a secondary electrospray ionization source of the present application from a first perspective;

[0023] Figure 4 It is a stereoscopic schematic diagram of a gas path system of a secondary electrospray ionization source of the present application from a second perspective;

[0024] Figure 5 It is a stereoscopic schematic diagram of a secondary electrospray ionization source of the present application from a first perspective;

[0025] Figure 6 It is a stereoscopic schematic diagram of a secondary electrospray ionization source of the present application from a second perspective;

[0026] Figure 7 It is a schematic diagram of the explosion of the internal structure of a secondary electrospray ionization source of the present application;

[0027] In the figure, 1. outer shell; 101. first side plate; 102. second side plate; 103. third side plate; 104. fixing plate; 105. fixing flange; 2. secondary electrospray ionization source gas path system; 201. buffer assembly; 202. first voltage regulating assembly; 203. switch assembly; 204. second voltage regulating assembly; 205. electrospray bottle assembly; 2051. electrospray bottle; 2052. capillary; 206. control assembly; 3. display module; 4. sample gas transmission tube; 5. ionization chamber. DETAILED DESCRIPTION

[0028] In the present utility model, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to the specific circumstances. The terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features.

[0029] In the present invention, unless otherwise clearly specified and limited, the first feature being "above" or "below" the second feature may include the first and second features being in direct contact, or the first and second features being in contact not directly but through another feature between them. Moreover, the first feature being "above", "above" and "above" the second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature includes the first feature being directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature. The terms "vertical", "horizontal", "left", "right", "above", "below" and similar expressions are for illustrative purposes only, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0030] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0031] Embodiment 1

[0032] like Figure 1-4A secondary electrospray ionization source gas path system 2 shown includes: a buffer component 201 for receiving input sheath gas and reducing turbulence of the sheath gas, a first pressure regulating component 202 for reducing the pressure of the sheath gas for the first time, a switch component 203 for controlling the connection or disconnection of the sheath gas pipeline, a second pressure regulating component 204 for reducing the pressure of the sheath gas for the second time, an electrospray bottle component 205 for evaporating liquid to form ions and transporting them to the mass spectrometer, and a control component 206; the output end of the buffer component 201 is connected to the input end of the first pressure regulating component 202; the output end of the first pressure regulating component 202 is connected to the input end of the switch component 203; the output end of the switch component 203 is connected to the input end of the second pressure regulating component 204; the output end of the second pressure regulating component 204 is connected to the input end of the electrospray bottle component 205; the switch component 203, the second pressure regulating component 204 and the electrospray bottle component 205 are all connected to the control component 206 for communication.

[0033] In the present application, the first pressure regulating component 202 is used to reduce the pressure of the sheath gas on a large scale for the first time, reducing the sheath gas originally 0.7MPa to about 0.3MPa. The switch component 203 can control the conduction or disconnection of the gas path under the control signal of the control component 206. The second pressure regulating component 204 is used to reduce the sheath gas pressure for the second time. In the present application, the second pressure regulating component 204 is connected to the control component 206 for communication. The second pressure regulating component 204 can accurately adjust the output gas pressure to maintain at 0.13MPa according to the control signal of the control component, and can provide a feedback signal to the control component 206, and adjust the pressure output by the second pressure regulating component 204 in real time according to the feedback signal. Finally, the sheath gas of 0.13MPa is delivered to the electrospray bottle component. In summary, the present application, by providing a switch component 203 and a second pressure regulating component 204 that can be connected to the control component 206 for communication, can use the control component 206 to automatically and accurately control the switch component 203 and the second pressure regulating component 204, so that the device can accurately maintain the sheath gas pressure in the pipeline at a required pressure value. It is known to those skilled in the art that by adjusting the opening of the first pressure regulating component 202, the output pressure of the first pressure regulating component 202 can be adaptively adjusted, and by adjusting the control signal of the control component 206, the output pressure of the second pressure regulating component can be adaptively adjusted, so the above-mentioned sheath gas pressure value should not constitute a specific limitation of the present application.

[0034] In one embodiment, the buffer assembly 201 specifically includes: a buffer chamber, the input end of the buffer chamber is connected to the sheath gas source through a hose; the output end of the buffer chamber is connected to the input end of the first pressure regulating assembly 202. In the present application, the buffer chamber is used to reduce the turbulence in the sheath gas, and the buffer chamber can also absorb the pressure pulsation and impact in the gas flow, so that the sheath gas flowing out of the hose can be more smoothly delivered to the downstream equipment.

[0035] In one embodiment, the first pressure regulating assembly 202 specifically includes: a manual pressure regulating valve; an input end of the manual pressure regulating valve is connected to the buffer chamber; and an output end of the manual pressure regulating valve is connected to an input end of the switch assembly 203. In the present application, the manual pressure regulating valve is used to reduce the pressure of the sheath gas for the first time. The pressure of the sheath gas flowing out of the sheath gas source is usually 0.7 MPa, and the manual pressure regulating valve can reduce the sheath gas from 0.7 MPa to 0.3 MPa.

[0036] In one embodiment, the switch assembly 203 specifically includes: a solenoid valve; the input end of the solenoid valve is connected to the output end of the manual pressure regulating valve; the output end of the solenoid valve is connected to the input end of the second pressure regulating assembly 204; the signal receiving end of the solenoid valve is communicatively connected to the control assembly 206. In the present application, the solenoid valve is a two-way two-position solenoid valve, two-way means that the solenoid valve has only two connection ports and can only realize the connection of one pipeline, and two-position means that the solenoid valve has only two working states, one is open and the other is closed. The signal receiving end of the solenoid valve is communicatively connected to the control assembly 206, and is used to control whether the sheath gas in the pipeline can pass under the control signal of the control assembly 206.

[0037] In one embodiment, the second pressure regulating component 204 specifically includes: a proportional valve; the input end of the proportional valve is connected to the output end of the solenoid valve; the output end of the proportional valve is connected to the electrospray bottle component 205; the signal receiving end of the proportional valve is communicatively connected to the control component 206. In the present application, the proportional valve can adjust the valve opening according to the control signal, so as to accurately control the flow rate of the sheath gas, so that the pressure of the sheath gas passing through the proportional valve is accurately controlled at 0.13MPa. The signal receiving end of the proportional valve is also used to receive the control signal of the control component 206, but unlike the solenoid valve, the proportional valve does not have only two working states, but can be nearly steplessly adjusted according to the control signal to accurately control the pressure of the gas passing through the proportional valve.

[0038] In one embodiment, the electrospray bottle assembly 205 includes: an electrospray bottle 2051 and a capillary 2052; the input end of the electrospray bottle 2051 is connected to the output end of the proportional valve; the output end of the electrospray bottle 2051 is connected to one end of the capillary 2052; the other end of the capillary 2052 is connected to the mass spectrometer. The electrospray bottle 2051 is used to squeeze the liquid in the bottle outward through the capillary 2052, and the liquid enters the ionization chamber 5 through the capillary 2052 to be ionized to form liquid ions, and then input into the mass spectrometer for mass analysis.

[0039] In one embodiment, the control component 206 uses a single chip microcomputer or other programmable chips, which can control the solenoid valve and the proportional valve to adjust according to the feedback signal to achieve precise control of the sheath gas pressure in the pipeline.

[0040] For other structures of the first embodiment, refer to the prior art.

[0041] Embodiment 2

[0042] Further, such as Figure 5 , Figure 6 Figure 7 As shown, the present application also provides a secondary electrospray ionization source, comprising: a housing 1, a display module 3, a sample gas transmission tube 4, an ionization chamber 5 and a secondary electrospray ionization source gas path system 2 as in Example 1; the display module 3, the ionization chamber 5 and the secondary electrospray ionization source gas path system 2 are all accommodated inside the housing 1; the display module 3 is communicatively connected to the control component 206; one end of the sample gas transmission tube 4 is connected to the first input end of the ionization chamber 5; the output end of the secondary electrospray ionization source gas path system 2 is connected to the second input end of the ionization chamber 5; and the output end of the ionization chamber 5 is connected to the mass spectrometer.

[0043] In the present application, the display module 3 also has a touch function, and people can call the control program pre-stored in the control component 206 through the touch screen to adjust the opening and closing of the solenoid valve or the proportional valve.

[0044] In one embodiment, the interior of the housing 1 is provided with: a fixing plate 104; a buffer chamber, a manual pressure regulating valve, a solenoid valve, an electrospray bottle 2051 module and a proportional valve are all fixedly connected to the fixing plate 104. In the present application, the fixing plate 104 is used to carry the secondary electrospray ionization source gas path system 2. Since there are many system pipelines for conveying sheath gas, in order to prevent the gas path system from being disturbed by the outside world and affecting the sheath gas conveying, a fixing plate 104 is arranged in the housing 1 to fix each component on the fixing plate 104 to avoid damage.

[0045] In one embodiment, the housing 1 includes a first side panel 101 and a second side panel 102, which are fixedly connected and arranged perpendicular to each other; the display module 3 is fixedly connected to the first side panel 101; and the handle of the manual pressure regulating valve passes through the second side panel 102 and extends to the outside of the housing 1. In the present application, the wrench of the manual valve is placed on the side panel of the housing 1, which can reduce the area of ​​the first side panel 101, that is, the front panel, and thus reduce the volume of the device.

[0046] In one embodiment, the housing 1 further includes a third side plate 103; the third side plate 103 and the first side plate 101 are fixedly connected and arranged perpendicular to each other; a fixing flange 105 for fixing the sample gas transmission tube 4 is fixedly arranged on the third side plate 103, and the fixing flange 105 is fixedly connected to the third side plate 103; the sample gas transmission tube 4 passes through the through hole on the fixing flange 105 and extends to the outside of the housing 1. The fixing flange 105 is used to clamp the sample gas delivery tube.

[0047] In one embodiment, a handle is fixedly provided on both sides of the housing 1 to facilitate moving the secondary electrospray ionization source.

[0048] In summary, this embodiment proposes a secondary electrospray ionization source, which uses the display module 3 to control the gas path components inside the device, thereby improving the integration of the device, reducing the difficulty of operating the device, and contributing to the integration and miniaturization of the device. The secondary electrospray ionization source also places the wrench of the manual pressure regulating valve on the side of the device, and there is no need to install a manual control knob on the front of the device, which helps to miniaturize the size of the device.

[0049] The present invention is not limited to the above-mentioned embodiments. If various changes or modifications to the present invention do not depart from the spirit and scope of the present invention, and if these changes and modifications fall within the claims of the present invention and the scope of equivalent technologies, the present invention is also intended to include these changes and modifications.

Claims

1. A secondary electrospray ionization source gas path system (2), characterized in that: include: A buffer component (201) for receiving input sheath gas and reducing turbulence of the sheath gas, a first pressure regulating component (202) for reducing the pressure of the sheath gas for the first time, a switch component (203) for controlling the connection or disconnection of the sheath gas pipeline, a second pressure regulating component (204) for reducing the pressure of the sheath gas for the second time, an electrospray bottle component (205) for evaporating liquid to form ions and transporting them to a mass spectrometer, and a control component (206); The output end of the buffer component (201) is connected to the input end of the first voltage regulating component (202); The output end of the first voltage regulating component (202) is connected to the input end of the switch component (203); The output end of the switch component (203) is connected to the input end of the second voltage regulating component (204); The output end of the second voltage regulating component (204) is connected to the input end of the electrospray bottle component (205); The switch component (203), the second voltage regulating component (204) and the electric spray bottle component (205) are all connected in communication with the control component (206).

2. A secondary electrospray ionization source gas path system (2) according to claim 1, characterized in that: The buffer component (201) specifically comprises: a buffer chamber, the input end of which is connected to a sheath gas source via a hose; and the output end of which is connected to the input end of a first pressure regulating component (202).

3. A secondary electrospray ionization source gas path system (2) according to claim 2, characterized in that: The first pressure regulating component (202) specifically comprises: a manual pressure regulating valve; an input end of the manual pressure regulating valve is connected to the buffer chamber; and an output end of the manual pressure regulating valve is connected to an input end of the switch component (203).

4. A secondary electrospray ionization source gas path system (2) according to claim 3, characterized in that: The switch component (203) specifically comprises: a solenoid valve; the input end of the solenoid valve is connected to the output end of the manual pressure regulating valve; the output end of the solenoid valve is connected to the input end of the second pressure regulating component (204); and the signal receiving end of the solenoid valve is communicatively connected to the control component.

5. A secondary electrospray ionization source gas path system (2) according to claim 4, characterized in that: The second pressure regulating component (204) specifically comprises: a proportional valve; the input end of the proportional valve is connected to the output end of the solenoid valve; the output end of the proportional valve is connected to the electric spray bottle component (205); and the signal receiving end of the proportional valve is communicatively connected to the control component.

6. A secondary electrospray ionization source gas path system (2) according to claim 5, characterized in that: The electrospray bottle assembly (205) comprises: an electrospray bottle (2051) and a capillary (2052); the input end of the electrospray bottle (2051) is connected to the output end of the proportional valve; the output end of the electrospray bottle (2051) is connected to one end of the capillary (2052); and the other end of the capillary (2052) is connected to a mass spectrometer.

7. A secondary electrospray ionization source, characterized in that: include: A housing (1), a display module (3), a sample gas transmission tube (4), an ionization chamber (5), and a secondary electrospray ionization source gas path system (2) as claimed in any one of claims 1 to 6; The display module (3), the ionization chamber (5) and the secondary electrospray ionization source gas path system (2) are all accommodated inside the housing (1); The display module (3) is communicatively connected to the control component (206); One end of the sample gas transmission tube (4) is connected to a first input end of the ionization chamber (5); The output end of the secondary electrospray ionization source gas path system (2) is connected to the second input end of the ionization chamber (5); The output end of the ionization chamber (5) is connected to a mass spectrometer.

8. A secondary electrospray ionization source gas path system (2) according to claim 7, characterized in that: The interior of the housing (1) is provided with: a fixing plate (104); The buffer chamber, the manual pressure regulating valve, the solenoid valve, the electrospray bottle (2051) module and the proportional valve are all fixedly connected to the fixed plate (104).

9. A secondary electrospray ionization source gas path system (2) according to claim 8, characterized in that: The housing (1) comprises a first side plate (101) and a second side plate (102), wherein the first side plate (101) and the second side plate (102) are fixedly connected and arranged perpendicular to each other; The display module (3) is fixedly connected to the first side panel (101); The handle of the manual pressure regulating valve passes through the second side plate (102) and extends to the outside of the housing (1).

10. A secondary electrospray ionization source gas path system (2) according to claim 9, characterized in that: The housing (1) further comprises a third side plate (103); the third side plate (103) and the first side plate (101) are fixedly connected and arranged perpendicular to each other; A fixing flange (105) for fixing the sample gas transmission tube (4) is fixedly arranged on the third side plate (103), and the fixing flange (105) is fixedly connected to the third side plate (103); The sample gas transmission tube (4) passes through the through hole on the fixing flange (105) and extends to the outside of the housing (1).

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