Gradient elution device for extract

Abstract

The utility model relates to the technical field of traditional Chinese medicine extracts, and discloses an extract gradient elution device which comprises a workbench, the bottom of the workbench is fixedly connected with a base, the upper surface of the workbench is fixedly connected with a high-concentration eluent box, the upper surface of the workbench is fixedly connected with a low-concentration eluent box, and the lower surface of the workbench is fixedly connected with a high-concentration eluent box. A mounting frame is fixedly connected to the upper surface of the workbench, and a cleaning assembly is arranged at the upper end of the workbench. A first motor drives a first transmission rod to rotate, drives a first fixing rod to rotate, enables a first belt wheel to rotate, drives a transmission belt to drive a second belt wheel to rotate, enables a second transmission rod to rotate, and drives a second fixing rod to rotate. And the first cleaning plate and the second cleaning plate rotate to clean the inner walls of the high-concentration eluent box and the low-concentration eluent box correspondingly, it is avoided that eluent remaining on the inner walls affects the subsequent use effect, the requirement for manual cleaning is reduced, and practicability is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of traditional Chinese medicine extracts, in particular to an extract gradient elution device. Background Art

[0002] Gradient elution is a commonly used chromatographic separation technique. It can effectively separate various compounds in a sample by changing the solvent composition in the mobile phase. In the analysis of traditional Chinese medicine extracts, the gradient elution method can help improve the separation efficiency, especially for the separation and analysis of traditional Chinese medicine components in complex systems.

[0003] The gradient elution device for recombinant protein purification and concentration with the publication number of CN206886997U injects eluents with different concentrations into the low-concentration eluent tank and the high-concentration eluent tank through the liquid inlet. Then, the flow regulating valve II is opened and the flow is controlled. The magnetic stirrer is started and the rotor rotates in the low-concentration eluent tank to stir the eluent in the low-concentration eluent tank. Subsequently, the flow regulating valve I is opened and the flow is controlled. The mixed eluent is discharged from the elbow pipe into the collecting cavity closest to the workbench side of the collecting tank. Then, the electric push rod is started to push the elbow pipe out of the water outlet pipe, and the eluents with different concentrations after mixing are discharged into the adjacent next collecting cavity for collection.

[0004] The gradient elution device for recombinant protein purification and concentration pushes the elbow pipe out of the water outlet pipe through the electric push rod and discharges the eluents with different concentrations after mixing into the adjacent next collecting cavity for collection. However, it is inconvenient to clean the inner walls of the low-concentration eluent tank and the high-concentration eluent tank, resulting in the remaining eluent after use, thus affecting the subsequent use effect. Content of the Utility Model

[0005] To solve the above technical problems, the utility model provides an extract gradient elution device.

[0006] The utility model is realized by adopting the following technical scheme: An extract gradient elution device includes a workbench. A base is fixedly connected to the bottom of the workbench. A high-concentration eluent tank is fixedly connected to the upper surface of the workbench. A low-concentration eluent tank is fixedly connected to the upper surface of the workbench. A mounting rack is fixedly connected to the upper surface of the workbench. A cleaning component is arranged at the upper end of the workbench. A liquid outlet pipe is arranged at the right end of the workbench. A collecting component is arranged at the right end of the workbench.

[0007] The cleaning component includes a first motor. The output end of the first motor is fixedly connected to a first transmission rod. The surface of the first transmission rod is fixedly connected to a first pulley. The inner wall of the first pulley is sleeved with a transmission belt. The bottom of the first transmission rod is fixedly connected to a first fixing rod. The surface of the first fixing rod is fixedly connected to a first cleaning plate. A second transmission rod is arranged at the right end of the first transmission rod. The surface of the second transmission rod is fixedly connected to a second pulley. The bottom of the second transmission rod is fixedly connected to a second fixing rod. The surface of the second transmission rod is fixedly connected to a second cleaning plate. The first pulley and the second pulley are connected by the transmission belt for transmission.

[0008] Through the above technical solution, the first motor drives the first transmission rod to rotate, drives the first fixing rod to rotate, makes the first pulley rotate, drives the transmission belt to drive the second pulley to rotate, makes the second transmission rod rotate, drives the second fixing rod to rotate, and makes the first cleaning plate and the second cleaning plate rotate to clean the inner walls of the high-concentration eluent tank and the low-concentration eluent tank respectively, improving the cleaning efficiency and thus the convenience during use.

[0009] As a further improvement of the above solution, the bottom of the first motor is fixedly connected to the top of the mounting frame. The first transmission rod passes through the mounting frame and is rotationally connected to the inner wall of the mounting frame. One end of the first fixing rod away from the first transmission rod is rotationally connected to the inner bottom wall of the high-concentration eluent tank.

[0010] As a further improvement of the above solution, one end of the first cleaning plate contacts the inner wall of the high-concentration eluent tank. The surface of the second transmission rod is rotationally connected to the inner wall of the mounting frame.

[0011] As a further improvement of the above solution, one end of the second fixing rod away from the second transmission rod is rotationally connected to the inner bottom wall of the low-concentration eluent tank. One end of the second cleaning plate contacts the inner wall of the low-concentration eluent tank.

[0012] Through the above technical solution, when the second cleaning plate rotates, it cleans the inner wall of the low-concentration eluent tank, thereby improving the subsequent use effect.

[0013] As a further improvement of the above solution, a connecting pipe is connected to the right end of the high-concentration eluent tank. The right end of the connecting pipe is connected to the low-concentration eluent tank. An outlet pipe is connected to the right end of the low-concentration eluent tank.

[0014] Through the above technical solution, the high-concentration eluent tank is connected to the low-concentration eluent tank through the connecting pipe and finally discharged through the outlet pipe.

[0015] As a further improvement of the above solution, the collection component includes a fixed frame, a collection box is arranged inside the fixed frame, a clamping groove is formed on the inner wall of the fixed frame, a bottom plate is arranged at the lower end of the fixed frame, and a second motor is fixedly connected to the upper surface of the bottom plate, and a transmission shaft is fixedly connected to the output end of the second motor.

[0016] Through the above technical solution, a plurality of collection boxes are provided. The motor drives the transmission shaft to rotate, so that the fixed frame rotates, thereby adjusting the position of the collection box.

[0017] As a further improvement of the above solution, the fixed frame is located at the lower end of the liquid outlet pipe. One end of the collection box is slidably connected to the inner wall of the clamping groove, and the top of the transmission shaft is fixedly connected to the bottom of the fixed frame.

[0018] Through the above technical solution, the collection box can be disassembled by moving it upward, and the operation is convenient and fast, which is convenient for removing the collection box for cleaning later.

[0019] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0020] The present utility model is provided with a cleaning component. Specifically, the first motor drives the first transmission rod to rotate, drives the first fixed rod to rotate, so that the first belt pulley rotates, drives the transmission belt to drive the second belt pulley to rotate, so that the second transmission rod rotates, drives the second fixed rod to rotate, so that the first cleaning plate and the second cleaning plate rotate to clean the inner walls of the high-concentration eluent tank and the low-concentration eluent tank respectively, avoiding the influence of the residual eluent on the inner wall on the subsequent use effect, and reducing the need for manual cleaning, thereby improving the practicability.

[0021] The present utility model is provided with a collection component. Specifically, the second motor drives the transmission shaft to rotate, so that the fixed frame rotates, and the position of the collection box can be adjusted. The eluent is discharged into the collection box through the liquid outlet pipe. The collection box is convenient for collecting eluents of different concentrations. By sliding the collection box upward, the collection box can be removed, which is convenient for cleaning the collection box later and improving the convenience during use. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 is a schematic diagram of the overall structure of the present utility model;

[0023] Figure 2 is a schematic sectional view of the overall structure of the present utility model;

[0024] Figure 3 is a schematic diagram of the cleaning component structure of the present utility model;

[0025] Figure 4 is a schematic diagram of the collection component structure of the present utility model;

[0026] Figure 5 This is a schematic side view of the overall structure of the present utility model.

[0027] Main symbol description:

[0028] 1. Workbench; 2. Base; 3. High-concentration eluent tank; 4. Low-concentration eluent tank; 5. Mounting frame; 6. Cleaning assembly; 601. First motor; 602. First transmission rod; 603. First pulley; 604. Transmission belt; 605. First fixing rod; 606. First cleaning plate; 607. Second transmission rod; 608. Second pulley; 609. Second fixing rod; 610. Second cleaning plate; 7. Connecting pipe; 8. Liquid outlet pipe; 9. Collection assembly; 901. Fixed frame; 902. Collection box; 903. Clamping groove; 904. Bottom plate; 905. Second motor; 906. Transmission shaft. Specific embodiments

[0029] Next, in combination with the accompanying drawings and specific embodiments, the present utility model will be further described. It should be noted that, on the premise of no conflict, any combination of the following-described embodiments or technical features can form a new embodiment.

[0030] Embodiment:

[0031] Please refer to Figures 1-5 An extract gradient elution device according to this embodiment includes a workbench 1. A base 2 is fixedly connected to the bottom of the workbench 1. A high-concentration eluent tank 3 is fixedly connected to the upper surface of the workbench 1. A low-concentration eluent tank 4 is fixedly connected to the upper surface of the workbench 1. A mounting frame 5 is fixedly connected to the upper surface of the workbench 1. A cleaning assembly 6 is provided at the upper end of the workbench 1. A liquid outlet pipe 8 is provided at the right end of the workbench 1. A collection assembly 9 is provided at the right end of the workbench 1;

[0032] The cleaning assembly 6 includes a first motor 601. The output end of the first motor 601 is fixedly connected to a first transmission rod 602. The surface of the first transmission rod 602 is fixedly connected to a first pulley 603. The inner wall of the first pulley 603 is sleeved with a transmission belt 604. The bottom of the first transmission rod 602 is fixedly connected to a first fixing rod 605. The surface of the first fixing rod 605 is fixedly connected to a first cleaning plate 606. The right end of the first transmission rod 602 is provided with a second transmission rod 607. The surface of the second transmission rod 607 is fixedly connected to a second pulley 608. The bottom of the second transmission rod 607 is fixedly connected to a second fixing rod 609. The surface of the second transmission rod 607 is fixedly connected to a second cleaning plate 610. The first pulley 603 and the second pulley 608 are connected by the transmission belt 604 for transmission. When it is necessary to clean the inner walls of the high-concentration eluent tank 3 and the low-concentration eluent tank 4, the first motor 601 is started. The first motor 601 drives the first transmission rod 602 to rotate, drives the first fixing rod 605 to rotate, makes the first pulley 603 rotate, drives the transmission belt 604 to drive the second pulley 608 to rotate, makes the second transmission rod 607 rotate, drives the second fixing rod 609 to rotate, and makes the first cleaning plate 606 and the second cleaning plate 610 rotate to clean the inner walls of the high-concentration eluent tank 3 and the low-concentration eluent tank 4 respectively, avoiding the influence of the residual eluent on the inner wall on the subsequent use effect, and reducing the need for manual cleaning, thus improving the practicability.

[0033] The bottom of the first motor 601 is fixedly connected to the top of the mounting frame 5. The first transmission rod 602 penetrates through the mounting frame 5 and is rotationally connected to the inner wall of the mounting frame 5. One end of the first fixing rod 605 away from the first transmission rod 602 is rotationally connected to the inner bottom wall of the high-concentration eluent tank 3.

[0034] One end of the first cleaning plate 606 is in contact with the inner wall of the high-concentration eluent tank 3. The surface of the second transmission rod 607 is rotationally connected to the inner wall of the mounting frame 5.

[0035] One end of the second fixing rod 609 away from the second transmission rod 607 is rotationally connected to the inner bottom wall of the low-concentration eluent tank 4. One end of the second cleaning plate 610 is in contact with the inner wall of the low-concentration eluent tank 4.

[0036] The right end of the high-concentration eluent tank 3 is communicated with a connecting pipe 7. The right end of the connecting pipe 7 is communicated with the low-concentration eluent tank 4. The right end of the low-concentration eluent tank 4 is communicated with a liquid outlet pipe 8.

[0037] The collection component 9 includes a fixed frame 901. Inside the fixed frame 901, there is a collection box 902. A clamping groove 903 is provided on the inner wall of the fixed frame 901. A bottom plate 904 is provided at the lower end of the fixed frame 901. A second motor 905 is fixedly connected to the upper surface of the bottom plate 904. The output end of the second motor 905 is fixedly connected to a transmission shaft 906. By starting the second motor 905, the second motor 905 drives the transmission shaft 906 to rotate, causing the fixed frame 901 to rotate, and thus the position of the collection box 902 can be adjusted. The eluent is discharged into the collection box 902 through the liquid outlet pipe 8. The collection box 902 facilitates the collection of eluents with different concentrations. By sliding the collection box 902 upward, the collection box 902 can be removed, which is convenient for subsequent cleaning of the collection box 902 and improves the convenience during use.

[0038] The fixed frame 901 is located at the lower end of the liquid outlet pipe 8. One end of the collection box 902 is slidably connected to the inner wall of the clamping groove 903. The top of the transmission shaft 906 is fixedly connected to the bottom of the fixed frame 901.

[0039] The implementation principle of an extract gradient elution device in an embodiment of this application is as follows: When it is necessary to clean the inner walls of the high-concentration eluent tank 3 and the low-concentration eluent tank 4, start the first motor 601. The first motor 601 drives the first transmission rod 602 to rotate, driving the first fixed rod 605 to rotate, causing the first pulley 603 to rotate, driving the transmission belt 604 to drive the second pulley 608 to rotate, causing the second transmission rod 607 to rotate, driving the second fixed rod 609 to rotate, and causing the first cleaning plate 606 and the second cleaning plate 610 to rotate to clean the inner walls of the high-concentration eluent tank 3 and the low-concentration eluent tank 4 respectively, avoiding the influence of the residual eluent on the inner wall on the subsequent use effect, reducing the need for manual cleaning, and improving the practicability. By starting the second motor 905, the second motor 905 drives the transmission shaft 906 to rotate, causing the fixed frame 901 to rotate, and thus the position of the collection box 902 can be adjusted. The eluent is discharged into the collection box 902 through the liquid outlet pipe 8. The collection box 902 facilitates the collection of eluents with different concentrations. By sliding the collection box 902 upward, the collection box 902 can be removed, which is convenient for subsequent cleaning of the collection box 902 and improves the convenience during use.

[0040] The above implementation manner is only the preferred implementation manner of the present utility model and cannot be used to limit the scope of protection of the present utility model. Any non-substantive changes and substitutions made by those skilled in the art based on the present utility model fall within the scope of protection required by the present utility model.

Claims

1. An extract gradient elution device, characterized in that: The workbench (1) comprises a workbench (1), the bottom of which is fixedly connected to a base (2), the upper surface of which is fixedly connected to a high-concentration eluent box (3), the upper surface of which is fixedly connected to a low-concentration eluent box (4), the upper surface of which is fixedly connected to a mounting frame (5), the upper end of which is provided with a cleaning assembly (6), the right end of which is provided with a liquid outlet pipe (8), and the right end of which is provided with a collecting assembly (9); The cleaning assembly (6) comprises a first motor (601), the output end of the first motor (601) is fixedly connected to a first transmission rod (602), the surface of the first transmission rod (602) is fixedly connected to a first pulley (603), the inner wall of the first pulley (603) is sleeved with a transmission belt (604), the bottom of the first transmission rod (602) is fixedly connected to a first fixing rod (605), the surface of the first fixing rod (605) is fixedly connected to a first cleaning plate (606), a second transmission rod (607) is provided at the right end of the first transmission rod (602), the surface of the second transmission rod (607) is fixedly connected to a second pulley (608), the bottom of the second transmission rod (607) is fixedly connected to a second fixing rod (609), the surface of the second transmission rod (607) is fixedly connected to a second cleaning plate (610), and the first pulley (603) and the second pulley (608) are connected in transmission via a transmission belt (604).

2. An extract gradient elution device as claimed in claim 1, characterized in that: The bottom of the first motor (601) is fixedly connected to the top of the mounting frame (5), the first transmission rod (602) passes through the mounting frame (5) and is rotatably connected to the inner wall of the mounting frame (5), and one end of the first fixed rod (605) away from the first transmission rod (602) is rotatably connected to the inner bottom wall of the high-concentration eluent box (3).

3. An extract gradient elution device as claimed in claim 1, characterized in that: One end of the first cleaning plate (606) is in contact with the inner wall of the high-concentration eluent box (3), and the surface of the second transmission rod (607) is rotatably connected to the inner wall of the mounting frame (5).

4. An extract gradient elution device as claimed in claim 1, characterized in that: One end of the second fixing rod (609) away from the second transmission rod (607) is rotatably connected to the inner bottom wall of the low-concentration eluent box (4), and one end of the second cleaning plate (610) is in contact with the inner wall of the low-concentration eluent box (4).

5. An extract gradient elution device as claimed in claim 1, characterized in that: The right end of the high-concentration eluent tank (3) is connected to a connecting pipe (7), the right end of the connecting pipe (7) is connected to a low-concentration eluent tank (4), and the right end of the low-concentration eluent tank (4) is connected to a liquid outlet pipe (8).

6. An extract gradient elution device as claimed in claim 1, characterized in that: The collecting assembly (9) comprises a fixed frame (901), a collecting box (902) is arranged inside the fixed frame (901), a clamping groove (903) is provided on the inner wall of the fixed frame (901), a bottom plate (904) is arranged at the lower end of the fixed frame (901), a second motor (905) is fixedly connected to the upper surface of the bottom plate (904), and a transmission shaft (906) is fixedly connected to the output end of the second motor (905).

7. An extract gradient elution device as claimed in claim 6, characterized in that: The fixed frame (901) is located at the lower end of the liquid outlet pipe (8), one end of the collecting box (902) is slidably connected to the inner wall of the clamping groove (903), and the top of the transmission shaft (906) is fixedly connected to the bottom of the fixed frame (901).

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