Cell scraping device for cell culture

Abstract

The utility model relates to the field of cell culture, and discloses a cell scraping device for cell culture, which comprises a bottom plate, the bottom of the bottom plate is fixedly connected with a first motor, the outer side of the output shaft of the first motor is fixedly connected with a protective box rotationally connected with the bottom plate, the protective box is internally provided with a clamping device, and the clamping device is fixedly connected with the bottom plate. The outer side of the clamping device is fixedly connected with two clamping plates which are symmetrically distributed left and right, the top of the bottom plate is fixedly connected with an L-shaped supporting plate, the bottom of the L-shaped supporting plate is fixedly connected with a first electric push rod, and the bottom of the first electric push rod is fixedly connected with a mounting plate; the bottom of the mounting plate is fixedly connected with a second electric push rod, and the front side of the second electric push rod is fixedly connected with a fixing frame slidably connected with the mounting plate. The cell scraping device for cell culture has the advantages that the culture dish can be fixed, and cells in the culture dish can be automatically scraped.

Description

Technical Field

[0001] The utility model relates to the field of cell cultivation, and specifically relates to a cell scraping device for cell cultivation. Background Technique

[0002] Cell culture, formally known as cell culture technology, refers to a method of simulating the in-vivo environment in vitro to enable cells to survive, grow, reproduce, and maintain their main structures and functions. Cell culture is an essential process in bioengineering technology and biological cloning technology. Most in-vitro cell cultures are carried out in culture dishes, and the cultured cells need to be scraped for subsequent cell detection.

[0003] Traditionally, the scraping of cells in a culture dish is done manually. However, since the outer box of the culture dish is relatively smooth, it is easy for the culture dish to slip when manually holding the culture dish and scraping it, resulting in damage to the cultured cells. Therefore, a cell scraping device for cell cultivation is proposed to solve the above problems. Content of the Utility Model

[0004] (1) Technical Problems to be Solved

[0005] Aiming at the deficiencies of the prior art, the utility model provides a cell scraping device for cell cultivation, which has the advantages of being able to fix the culture dish and automatically scrape the cells in the culture dish, and solves the problem that in the traditional situation, the scraping of cells in the culture dish is done manually, but since the outer box of the culture dish is relatively smooth, it is easy for the culture dish to slip when manually holding the culture dish and scraping it, resulting in damage to the cultured cells.

[0006] (2) Technical Solutions

[0007] The technical solution of the utility model to solve the above technical problems is as follows: A cell scraping device for cell cultivation includes a bottom plate. A first motor is fixedly connected to the bottom of the bottom plate. The outer side of the output shaft of the first motor is fixedly connected to a protective box rotatably connected to the bottom plate. A clamping device is arranged inside the protective box. The outer side of the clamping device is fixedly connected to two clamping plates distributed symmetrically left and right. An L-shaped support plate is fixedly connected to the top of the bottom plate. A first electric push rod is fixedly connected to the bottom of the L-shaped support plate. The bottom of the first electric push rod is fixedly connected to a mounting plate. A second electric push rod is fixedly connected to the bottom of the mounting plate. The front side of the second electric push rod is fixedly connected to a fixing frame slidably connected to the mounting plate. A scraper is arranged inside the fixing frame.

[0008] The beneficial effects of the utility model are as follows:

[0009] The cell scraping device for cell cultivation has the advantages of being able to fix the culture dish and automatically scrape the cells in the culture dish.

[0010] On the basis of the above technical solution, the present utility model can be further improved as follows.

[0011] Further, an annular groove is provided inside the bottom plate, and the bottom of the protection box is fixedly connected with two arc-shaped plates that are both slidably connected to the annular groove.

[0012] The beneficial effect of adopting the above further solution is to assist the rotation of the protection box, provide support for the protection box, and prevent the protection box from shifting and skewing.

[0013] Further, the clamping device includes a second motor fixedly connected to the outside of the protection box, a left-right screw rod that is fixedly connected to the outside of the output shaft of the second motor and is rotatably connected to the inside of the protection box, and two transmission blocks that are threadedly connected to the outside of the left-right screw rod and are respectively fixedly connected to the two clamping plates.

[0014] The beneficial effect of adopting the above further solution is that it can clamp and fix the culture dish to prevent the culture dish from slipping during the scraping process.

[0015] Further, the opposite sides of the two clamping plates are both arc-shaped, and buffer pads are provided on the opposite surfaces of the two clamping plates.

[0016] The beneficial effect of adopting the above further solution is that the clamping plates can better adapt to the curvature of the culture dish. While the buffer pads prevent the culture dish from being damaged, they also increase the friction between the culture dish and the clamping plates, making the fixation of the culture dish more stable.

[0017] Further, a threaded hole is provided inside the fixed frame, a bolt knob is threadedly connected to the inside of the threaded hole, and a pressing plate that is located inside the fixed frame and contacts the scraper is rotatably connected to the outside of the bolt knob.

[0018] The beneficial effect of adopting the above further solution is that it can fix the scraper, facilitating the fixation and removal of the scraper.

[0019] Further, a chute is provided inside the mounting plate, and a sliding plate that is slidably connected to the chute is fixedly connected to the top of the fixed frame.

[0020] The beneficial effect of adopting the above further solution is to assist the movement of the fixed frame and provide a pulling force for the fixed frame to prevent the fixed frame and the second electric push rod from being pulled and deformed. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1Schematic diagram of the front structure of the present utility model;

[0022] Figure 2 Schematic diagram of the clamping device structure of the present utility model;

[0023] Figure 3 Schematic diagram of the mounting plate structure of the present utility model;

[0024] Figure 4 Schematic diagram of the fixed frame structure of the present utility model.

[0025] In the figure: 1, bottom plate; 2, first motor; 3, protective box; 4, clamping device; 41, second motor; 42, left - right screw rod; 43, transmission block; 5, clamping plate; 6, L - shaped support plate; 7, first electric push rod; 8, mounting plate; 9, second electric push rod; 10, fixed frame; 11, scraper; 12, arc plate; 13, buffer pad; 14, bolt knob; 15, pressing plate; 16, sliding plate. Specific embodiments

[0026] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

[0027] In the embodiment, it is given by Figures 1-4 A cell scraping device for cell cultivation. The present utility model includes a bottom plate 1. A first motor 2 is fixedly connected to the bottom of the bottom plate 1. The outer side of the output shaft of the first motor 2 is fixedly connected with a protective box 3 rotatably connected to the bottom plate 1. A clamping device 4 is arranged inside the protective box 3. The outer side of the clamping device 4 is fixedly connected with two clamping plates 5 distributed symmetrically left and right. The top of the bottom plate 1 is fixedly connected with an L - shaped support plate 6. The bottom of the L - shaped support plate 6 is fixedly connected with a first electric push rod 7. The bottom of the first electric push rod 7 is fixedly connected with a mounting plate 8. The bottom of the mounting plate 8 is fixedly connected with a second electric push rod 9. The front side of the second electric push rod 9 is fixedly connected with a fixed frame 10 slidably connected to the mounting plate 8. A scraper 11 is arranged inside the fixed frame 10.

[0028] Among them, an annular groove is arranged inside the bottom plate 1. The bottom of the protective box 3 is fixedly connected with two arc plates 12 both of which are slidably connected to the annular groove.

[0029] In this embodiment, during the actual use process, when the first motor 2 drives the rotation of the protective box 3, the two arc plates 12 below the protective box 3 will also slide in the annular groove to stabilize the rotation direction of the protective box 3.

[0030] Among them, the clamping device 4 includes a second motor 41 fixedly connected to the outside of the protective box 3. A left - right screw rod 42 rotatably connected to the inside of the protective box 3 is fixedly connected to the outside of the output shaft of the second motor 41. The outside of the left - right screw rod 42 is threadedly connected with two transmission blocks 43 respectively fixedly connected to two clamping plates 5.

[0031] In this embodiment, during actual use, the second motor 41 has its own internal power supply. The start of the second motor 41 will drive the left - right screw rod 42 to rotate, and under the action of the thread, the two transmission blocks 43 will move in opposite directions, thereby driving the two clamping plates 5 to move.

[0032] Among them, the opposite sides of the two clamping plates 5 are both arc - shaped, and buffer pads 13 are arranged on the opposite surfaces of the two clamping plates 5.

[0033] In this embodiment, during actual use, the relative movement of the two clamping plates 5 will clamp and fix the culture dish, and the fixation will be made more stable under the action of the buffer pads 13.

[0034] Among them, a threaded hole is opened inside the fixed frame 10. A bolt knob 14 is threadedly connected to the inside of the threaded hole. A pressing plate 15 located inside the fixed frame 10 and in contact with the scraper 11 is rotatably connected to the outside of the bolt knob 14.

[0035] In this embodiment, during actual use, when the bolt knob 14 is turned, the pressing plate 15 will move inward and press the scraper 11, and fix the scraper 11 inside the fixed frame 10.

[0036] Among them, a chute is opened inside the mounting plate 8. A sliding plate 16 fixedly connected to the top of the fixed frame 10 is slidably connected to the chute.

[0037] In this embodiment, during actual use, when the second electric push rod 9 drives the fixed frame 10 to move, the sliding plate 16 will move in the chute, stabilizing the moving direction of the fixed frame 10.

[0038] Working principle:

[0039] First, place the petri dish between two clamping plates 5. Then, start the second motor 41 to clamp and fix it with the two clamping plates 5. Adjust the height of the scraper 11 through the first electric push rod 7 so that the scraping blade position of the scraper 11 can just scrape the bottom surface of the petri dish. Start the first motor 2 to drive the petri dish to rotate by the protective box 3. During the rotation, the scraper 11 will scrape the petri dish. By adjusting the extension length of the second electric push rod 9, the scraping radius of the scraper 11 can be adjusted so that the scraper 11 can scrape the petri dish sufficiently. The scraper 11 can be removed by turning the bolt knob 14 for manual scraping or cleaning the scraper 11.

[0040] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising an..." does not exclude the presence of additional identical elements in the process, method, article or device comprising the element.

[0041] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell scraping device for cell culture, comprising a bottom plate (1), characterized in that: The bottom of the base plate (1) is fixedly connected to a first motor (2); the outer side of the output shaft of the first motor (2) is fixedly connected to a protective box (3) rotatably connected to the base plate (1); a clamping device (4) is arranged inside the protective box (3); two clamping plates (5) symmetrically distributed on the left and right are fixedly connected to the outer side of the clamping device (4); the top of the base plate (1) is fixedly connected to an L-shaped support plate (6); the bottom of the L-shaped support plate (6) is fixedly connected to a first electric push rod (7); the bottom of the first electric push rod (7) is fixedly connected to a mounting plate (8); the bottom of the mounting plate (8) is fixedly connected to a second electric push rod (9); the front side of the second electric push rod (9) is fixedly connected to a fixed frame (10) slidably connected to the mounting plate (8); a scraper (11) is arranged inside the fixed frame (10).

2. A cell scraping device for cell culture according to claim 1, characterized in that: An annular groove is provided inside the bottom plate (1), and two arc-shaped plates (12) are fixedly connected to the bottom of the protection box (3) and are both slidably connected to the annular groove.

3. A cell scraping device for cell culture according to claim 1, characterized in that: The clamping device (4) comprises a second motor (41) fixedly connected to the outer side of the protective box (3); the outer side of the output shaft of the second motor (41) is fixedly connected to left and right screw rods (42) which are rotatably connected to the inside of the protective box (3); the outer sides of the left and right screw rods (42) are connected to two transmission blocks (43) respectively fixedly connected to the two clamping plates (5) through threaded transmission.

4. A cell scraping device for cell culture according to claim 1, characterized in that: The opposite sides of the two clamping plates (5) are both arranged in an arc shape, and the opposite surfaces of the two clamping plates (5) are both provided with a buffer pad (13).

5. A cell scraping device for cell culture according to claim 1, characterized in that: A threaded hole is provided inside the fixing frame (10), a bolt knob (14) is threadedly connected inside the threaded hole, and a pressure plate (15) located inside the fixing frame (10) and in contact with the scraper (11) is rotatably connected outside the bolt knob (14).

6. A cell scraping device for cell culture according to claim 1, characterized in that: A sliding groove is provided inside the mounting plate (8), and a sliding plate (16) is fixedly connected to the top of the fixing frame (10) and is slidably connected to the sliding groove.

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