Bacterial liquid extraction device for rapidly detecting activity of anaerobic microorganisms

Abstract

The utility model relates to the technical field of anaerobic remediation of microorganisms in contaminated soil, in particular to a bacterial liquid extraction device for rapidly detecting the activity of anaerobic microorganisms, which comprises a main body, a piston, a pushing handle, a gas supply component, a stainless steel wire mesh, two gas intercommunication channels, a first filter membrane and a discharge component, the main body is provided with an inert gas cabin, an extraction cabin and an injection port, the piston is in sliding connection with the extraction cabin, the pushing handle is fixedly connected with the piston, the stainless steel wire mesh and the first filter membrane are arranged in the extraction cabin, the lower part of the extraction cabin is provided with an extraction liquid access space, and the gas supply assembly is communicated with the inert gas cabin; the inert gas cabin is communicated with the extraction cabin through two gas intercommunication channels, so that the technical problems that in the prior art, the extraction efficiency of a bacterial liquid extraction device used for detecting the growth activity of microorganisms on site soil microorganisms is low, the activity information of anaerobic microorganisms cannot be rapidly obtained, and carrying is inconvenient are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of anaerobic remediation of contaminated soil by microorganisms, in particular to a bacterial liquid extraction device for rapid detection of anaerobic microorganism activity. Background Technique

[0002] With the update of urban functional areas and the adjustment of industrial structure, a large amount of contaminated site soil has been left behind after the relocation of factories and enterprises such as petroleum, steel, and chemical industries; the residual organic matter and heavy metal pollution in these contaminated site soils seriously threaten the ecological environment, have indirect and direct toxic effects on animals and plants in the area, and can affect human health and safety through the food chain; the contradiction between land supply in China is prominent, forcing these contaminated sites to be redeveloped and utilized; therefore, it is very necessary to repair the contaminated site soil with efficient, economical, and safe repair technologies; Microbial remediation is one of the commonly used soil remediation methods for contaminated sites, with the advantages of being green and economical, having little secondary pollution, and being able to be disposed in situ; Anaerobic microorganisms are the main bacteria for degrading pollutants in deep-site soil, and can degrade petroleum alkanes, pesticides, detergents, etc. into simple inorganic substances through reactions such as dehalogenation, reduction, and ring cleavage; However, in the process of microbial remediation, problems such as low microbial growth activity, slow mass transfer process in the soil environment, and low microbial abundance in deep-site soil will also occur, which will also lead to low microbial remediation efficiency; Therefore, rapid detection of the growth activity during the process of microbial degradation of pollutants can effectively control the microbial remediation process.

[0003] At present, the commonly used methods for detecting microbial growth activity include dilution plate counting, community-level physiological profiling analysis, microbial biomass analysis, phospholipid fatty acid analysis, molecular ecology techniques, etc. Dilution plate counting and community-level physiological profiling analysis rely on microbial growth and its activity in the environment; Microbial biomass analysis, phospholipid fatty acid analysis, and molecular ecology techniques rely on the accuracy of sample pretreatment methods and analytical instruments. Therefore, the pretreatment of microbial samples is the key to monitoring, regulating, and predicting the microbial remediation efficiency of contaminated soil in microbial remediation sites; Biological enzyme activity is one of the important biological indicators of soil pollution, is significantly correlated with the physical and chemical indicators of soil quality, and is very sensitive to changes in the soil ecological environment; At present, relatively mature soil enzyme detection indicators include soil urease, dehydrogenase, invertase, phosphatase, etc.

[0004] The existing bacterial liquid extraction device used for detecting microbial growth activity has a slow extraction efficiency for site soil microorganisms, resulting in the inability to quickly obtain information on anaerobic microorganism activity and being inconvenient to carry. Content of the Utility Model

[0005] The purpose of the present utility model is to provide a bacterial liquid extraction device for rapid detection of anaerobic microbial activity, aiming to solve the technical problems in the prior art that the bacterial liquid extraction device used for detecting the growth activity of microorganisms has a slow extraction efficiency for site soil microorganisms, resulting in the inability to quickly obtain information on anaerobic microbial activity and being inconvenient to carry.

[0006] To achieve the above object, a bacterial liquid extraction device for rapid detection of anaerobic microbial activity adopted by the present utility model includes a main body, a piston, a pushing handle, a gas supply assembly, a stainless steel wire mesh, two gas intercommunication channels, a first filter membrane, and a discharging assembly. An inert gas chamber, an extraction chamber, and an injection port are provided on the main body. The piston is slidably connected to the extraction chamber, the pushing handle is fixedly connected to the piston, the stainless steel wire mesh and the first filter membrane are both arranged in the extraction chamber, an extraction liquid storage space is arranged at the lower part of the extraction chamber, the gas supply assembly is communicated with the inert gas chamber, and the inert gas chamber is communicated with the extraction chamber through the two gas intercommunication channels. The discharging assembly is arranged below the extraction liquid storage space.

[0007] Among them, the gas supply assembly includes an inert gas cylinder, a gas connection pipeline, and a gas connection valve. Both ends of the gas connection pipeline are communicated with the inert gas cylinder and the inert gas chamber, and the gas connection valve is arranged on the gas connection pipeline.

[0008] Among them, the discharging assembly includes an iron sheet, a second filter membrane, a capillary needle tube connection channel, a capillary needle tube, and an iron sheet switch valve. The capillary needle tube connection channel is arranged between the filter membrane and the capillary needle tube. The iron sheet is arranged above the second filter membrane, and the iron sheet switch valve is used to open the iron sheet.

[0009] Among them, the bacterial liquid extraction device for rapid detection of anaerobic microbial activity further includes a pressure gauge, and the pressure gauge is arranged on the main body.

[0010] Among them, the bacterial liquid extraction device for rapid detection of anaerobic microbial activity further includes an automatic push-pull device, and the automatic push-pull device is connected to the pushing handle.

[0011] Among them, the aperture of the stainless steel wire mesh is 250 - 300 mesh, the first filter membrane is a single-layer 0.45μm filter membrane, and the second filter membrane is a double-layer 0.22μm filter membrane.

[0012] A bacterial liquid extraction device for rapid detection of anaerobic microorganism activity of the present utility model. The stainless steel wire mesh is made of 304 stainless steel material. During specific use, first, take out the piston from the extraction chamber, place the soil sample to be extracted on the stainless steel wire mesh, install the piston into the extraction chamber, then open the gas connection valve and the iron sheet switch valve. Inert gas enters the device through the gas connection channel. Wait until the inert gas fills the inert gas chamber and open the gas interconnection channel to make the gas fill the extraction chamber and the extraction liquid storage space. After the air pressure is stable, close the iron sheet switch valve and the gas interconnection channel. After the gas stays for about 30 minutes, sterilize the extraction liquid to be used through the injection port and then put it into the extraction liquid storage space. Invert the device so that the extraction liquid enters the space between the piston and the stainless steel wire mesh. After staying for about 30 seconds, drive the piston to move up and down by pushing the handle. Repeat this for about 25 minutes, then place the device upright so that the extraction liquid passes through the stainless steel wire mesh and the first filter membrane into the extraction liquid storage space. Subsequently, after installing the second filter membrane, the capillary needle tube connection channel and the capillary needle tube, close the gas connection valve. After the air pressure returns to normal, open the iron sheet switch valve. The iron sheet changes from the closed state to the open state. At this time, the extraction liquid flows out from the storage space, passes through the second filter membrane and the capillary needle tube connection channel, and then is discharged through the capillary needle tube and can be directly measured. In this way, it solves the technical problems in the prior art that the bacterial liquid extraction device used for detecting the growth activity of microorganisms has a slow extraction efficiency for the soil microorganisms in the site, resulting in the inability to quickly obtain the anaerobic microorganism activity information and being inconvenient to carry.

[0013] The present utility model has the following beneficial effects: 1. Set up an anaerobic microorganism extraction device, configure conditions suitable for the growth of anaerobic bacteria according to the characteristics of anaerobic microorganisms in the soil. After multiple extractions, the extraction liquid can extract anaerobic microorganisms to reflect the activity of anaerobic microorganisms in the polluted site.

[0014] 2. Set up two sets of filter membrane devices, namely a 0.45μm filter membrane and a 0.22μm filter membrane, to strictly control that there is no microbial cross-contamination between the internal devices and prevent the influence of front-end microbial contamination on the anaerobic microorganism extraction liquid flowing out of the capillary needle tube.

[0015] 3. Through this device, the anaerobic microorganisms in the soil of the polluted site can be extracted conveniently and quickly to obtain the anaerobic microorganism activity information quickly. At the same time, this device can be carried into the site conveniently to guide the on-site construction. Description of the Drawings

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0017] Figure 1 It is a schematic structural diagram of the bacterial liquid extraction device for rapid detection of anaerobic microorganism activity of the present invention.

[0018] 101 - Main body, 102 - Piston, 103 - Push handle, 104 - Stainless steel wire mesh, 105 - First filter membrane, 106 - Inert gas cylinder, 107 - Gas connection pipe, 108 - Gas communication valve, 109 - Iron sheet, 110 - Second filter membrane, 111 - Capillary needle tube connection channel, 112 - Capillary needle tube, 113 - Iron sheet switch valve, 114 - Pressure gauge, 115 - Automatic push - pull device, 116 - Inert gas chamber, 117 - Extraction chamber, 118 - Injection port, 119 - Extraction liquid storage space, 120 - Gas inter - communication channel. Detailed implementation manners

[0019] The following will describe in detail the embodiments of the present invention. The examples of the embodiments are shown in the drawings. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present invention, and should not be construed as a limitation to the present invention.

[0020] Please refer to Figure 1 , Figure 1 It is a schematic structural diagram of the bacterial liquid extraction device for rapid detection of anaerobic microorganism activity of the present invention.

[0021] The present invention provides a bacterial liquid extraction device for rapid detection of anaerobic microorganism activity, including a main body 101, a piston 102, a push handle 103, a gas supply assembly, a stainless steel wire mesh 104, two gas inter - communication channels 120, a first filter membrane 105 and a discharge assembly. An inert gas chamber 116, an extraction chamber 117 and an injection port 118 are arranged on the main body 101. The piston 102 is slidably connected to the extraction chamber 117. The push handle 103 is fixedly connected to the piston 102. The stainless steel wire mesh 104 and the first filter membrane 105 are both arranged in the extraction chamber 117. An extraction liquid storage space 119 is arranged at the lower part of the extraction chamber 117. The gas supply assembly is communicated with the inert gas chamber 116. The inert gas chamber 116 and the extraction chamber 117 are communicated through the two gas inter - communication channels 120. The discharge assembly is arranged below the extraction liquid storage space 119;

[0022] The air supply assembly includes an inert gas cylinder 106, a gas connection pipe 107, and a gas connection valve 108. Both ends of the gas connection pipe 107 are connected to the inert gas cylinder 106 and the inert gas chamber 116, and the gas connection valve 108 is arranged on the gas connection pipe 107;

[0023] The discharging assembly includes an iron sheet 109, a second filter membrane 110, a capillary needle connection channel 111, a capillary needle 112, and an iron sheet switch valve 113. The capillary needle connection channel 111 is arranged between the filter membrane and the capillary needle 112. The iron sheet 109 is arranged above the second filter membrane 110, and the iron sheet switch valve 113 is used to open the iron sheet 109;

[0024] The aperture of the stainless steel wire mesh 104 is 250 - 300 mesh. The first filter membrane 105 is a single-layer 0.45μm filter membrane, and the second filter membrane 110 is a double-layer 0.22μm filter membrane.

[0025] For this specific embodiment, the stainless steel wire mesh 104 is made of 304 stainless steel material. During specific use, first, the piston 102 is taken out of the extraction chamber 117, and the soil sample to be extracted is placed on the stainless steel wire mesh 104. Then, the piston 102 is installed into the extraction chamber 117. After that, the gas connection valve and the iron sheet switch valve 113 are opened, and the inert gas enters the device through the gas connection channel. Wait until the inert gas fills the inert gas chamber 116 and open the gas interconnection channel 120 to make the gas fill the extraction chamber 117 and the extraction liquid storage space 119. After the air pressure is stable, close the iron sheet switch valve 113 and the gas interconnection channel 120. After the gas stays for about 30 minutes, the extraction liquid to be used is sterilized through the injection port 118 and then enters the extraction liquid storage space 119. Invert the device so that the extraction liquid enters the space between the piston 102 and the stainless steel wire mesh 104. After staying for about 30 seconds, drive the piston 102 to move up and down by pushing the handle 103. Repeat this for about 25 minutes, then place the device upright so that the extraction liquid passes through the stainless steel wire mesh 104 and the first filter membrane 105 and enters the extraction liquid storage space 119. Subsequently, after installing the second filter membrane 110, the capillary needle tube connection channel 111, and the capillary needle tube 112, close the gas connection valve. After the air pressure returns to normal, open the iron sheet switch valve 113, and the iron sheet 109 changes from the closed state to the open state. At this time, the extraction liquid flows out of the extraction liquid storage space 119, passes through the second filter membrane 110 and the capillary needle tube connection channel 111, and is then discharged through the capillary needle tube 112, and the extraction liquid can be directly measured. In this way, it solves the technical problems in the prior art that the extraction efficiency of the bacterial liquid extraction device used for detecting the growth activity of microorganisms in the field soil is slow, resulting in the inability to quickly obtain the anaerobic microorganism activity information and it is not convenient to carry.

[0026] Among them, the bacterial liquid extraction device for rapid detection of anaerobic microorganism activity further includes a pressure gauge 114, and the pressure gauge 114 is arranged on the main body 101.

[0027] For this specific embodiment, the pressure gauge 114 is used to monitor the air pressure in the inert gas chamber 116, and judge whether the air pressure returns to normal according to the pressure gauge 114.

[0028] Secondly, the bacterial liquid extraction device for rapid detection of anaerobic microorganism activity further includes an automatic pusher 115, and the automatic pusher 115 is connected to the push handle 103.

[0029] For this specific embodiment, the automatic pusher 115 can drive the push handle 103 to move reciprocally, thereby reducing the labor intensity of the staff.

[0030] Using the bacterial liquid extraction device for rapid detection of anaerobic microorganism activity in this embodiment, the stainless steel wire mesh 104 is made of 304 stainless steel material. When specifically used, first take out the piston 102 from the extraction chamber 117, place the soil sample to be extracted on the stainless steel wire mesh 104, install the piston 102 into the extraction chamber 117, then open the gas connection valve and the iron sheet switch valve 113. The inert gas enters the device through the gas connection channel. Wait until the inert gas fills the inert gas chamber 116 and open the gas interconnection channel 120 to make the gas fill the extraction chamber 117 and the extraction liquid storage space 119. After the air pressure is stable, close the iron sheet switch valve 113 and the gas interconnection channel 120. After the gas stays for about 30 minutes, sterilize the extraction liquid to be used through the injection port 118 and then pour it into the extraction liquid storage space 119; invert the device so that the extraction liquid enters the space between the piston 102 and the stainless steel wire mesh 104. After staying for about 30 seconds, drive the piston 102 to move up and down by pushing the handle 103. Repeat this for about 25 minutes, then place the device upright so that the extraction liquid passes through the stainless steel wire mesh 104 and the first filter membrane 105 and enters the extraction liquid storage space 119. Subsequently, after installing the second filter membrane 110, the capillary needle tube connection channel 111 and the capillary needle tube 112, close the gas connection valve. After the air pressure returns to normal, open the iron sheet switch valve 113. The iron sheet 109 changes from the closed state to the open state. At this time, the extraction liquid flows out of the extraction liquid storage space 119, passes through the second filter membrane 110 and the capillary needle tube connection channel 111, and then is discharged through the capillary needle tube 112, and the extraction liquid can be directly measured. In this way, it solves the technical problems in the prior art that the bacterial liquid extraction device used for detecting the growth activity of microorganisms has a slow extraction efficiency for site soil microorganisms, resulting in the inability to quickly obtain the anaerobic microorganism activity information and being inconvenient to carry.

[0031] The utility model has the following beneficial effects: 1. An anaerobic microorganism extraction device is set up. According to the characteristics of anaerobic microorganisms in the soil, conditions suitable for the growth of anaerobic bacteria are configured. After multiple extractions, the extraction liquid can extract anaerobic microorganisms to reflect the activity of anaerobic microorganisms in the polluted site;

[0032] 2. Two sets of filter membrane devices, namely a 0.45μm filter membrane and a 0.22μm filter membrane, are set up to strictly control that there is no microbial cross - contamination between the internal devices, and also prevent the influence of front - end microbial contamination on the anaerobic microorganism extraction liquid flowing out of the capillary needle tube 112;

[0033] 3. By using this device to extract anaerobic microorganisms from the soil of contaminated sites, the soil microorganisms of the site can be conveniently and quickly extracted to rapidly obtain the activity information of anaerobic microorganisms. At the same time, this device can be conveniently carried into the site to guide the on-site construction.

[0034] The above-disclosed is only a preferred embodiment of the present utility model. Of course, it cannot be used to limit the scope of rights of the present utility model. Those of ordinary skill in the art can understand all or part of the processes of implementing the above embodiments, and the equivalent changes made according to the claims of the present utility model still fall within the scope covered by the utility model.

Claims

1. A bacterial liquid extraction device for rapid detection of anaerobic microbial activity, characterized in that: The invention comprises a main body, a piston, a pushing handle, a gas supply assembly, a stainless steel wire mesh, two gas interconnecting channels, a first filter membrane and a discharging assembly; the main body is provided with an inert gas cabin, an extraction bin and an injection port; the piston is slidably connected to the extraction bin, the pushing handle is fixedly connected to the piston, the stainless steel wire mesh and the first filter membrane are both arranged in the extraction bin, an extraction liquid storage and access space is arranged at the lower part of the extraction bin, the gas supply assembly is communicated with the inert gas cabin, the inert gas cabin and the extraction bin are communicated with each other through the two gas interconnecting channels, and the discharging assembly is arranged below the extraction liquid storage and access space.

2. The bacterial liquid extraction device for rapid detection of anaerobic microbial activity according to claim 1, characterized in that: The gas supply assembly includes an inert gas bottle, a gas connecting pipe and a gas connecting valve. Both ends of the gas connecting pipe are connected to the inert gas bottle and the inert gas cabin. The gas connecting valve is arranged on the gas connecting pipe.

3. The bacterial liquid extraction device for rapid detection of anaerobic microbial activity according to claim 2, characterized in that: The discharging component includes an iron sheet, a second filter membrane, a capillary needle tube connecting channel, a capillary needle tube and an iron sheet switch valve. The capillary needle tube connecting channel is arranged between the filter membrane and the capillary needle tube, the iron sheet is arranged above the second filter membrane, and the iron sheet switch valve is used to open the iron sheet.

4. The bacterial liquid extraction device for rapid detection of anaerobic microbial activity according to claim 3, characterized in that: The bacterial liquid extraction device for rapid detection of anaerobic microbial activity also includes a pressure gauge, which is arranged on the main body.

5. The bacterial liquid extraction device for rapid detection of anaerobic microbial activity according to claim 4, characterized in that: The bacterial liquid extraction device for rapid detection of anaerobic microbial activity also includes an automatic push-pull device, which is connected to the push handle.

6. The bacterial liquid extraction device for rapid detection of anaerobic microbial activity according to claim 5, characterized in that: The pore size of the stainless steel wire mesh is 250-300 meshes, the first filter membrane is a single-layer 0.45 μm filter membrane, and the second filter membrane is a double-layer 0.22 μm filter membrane.

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