Uncovering-free culture dish for cell scratch experiment photographing
Through the experiment of taking photos of cell scratches of transparent glass materials, the uncovered petri dish was solved in the prior art, the sterile environment damage, affected cell growth status and unstable image quality caused by frequent opening of the upper cover, and the high-quality shooting of cell images without opening the upper cover is achieved, reducing the operating burden.
Patent Information
- Application Number
- CN202421753962.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-23
- Publication Date
- 2025-05-27
- Estimated Expiration
- 2034-07-23
AI Technical Summary
In existing cell scratch experiments, it is necessary to frequently open the upper lid of the Petri dish for microscope photography, resulting in damage to the sterile environment, affected cell growth status, unstable image quality and increased operating burden.
A culture dish with no open-cover photography for cell scratches was designed. The base and bottom walls and top walls of the upper cover were respectively made of transparent glass materials, allowing cells to be photographed by a microscope without opening the upper cover.
By using a Petri dish with transparent glass material, the impact of cell contamination and growth state is reduced, image quality is improved, operation burden is reduced, and the impact on external lighting conditions is reduced.
Smart Images
Figure CN222907927U_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of cell scratch experiments, and in particular to a cell scratch experiment photography-free culture dish. Background Art
[0002] The wound healing assay is an in vitro experimental method commonly used to study cell migration and can be used to detect the ability of tumor cells that grow on a wall to metastasize. Specifically, during the experiment, when the cells grow to a fused monolayer state, a scratch tool is used to artificially create a blank area on the fused monolayer of cells. As the culture time increases, the cells at the edge of the scratch gradually enter the blank area, causing the scratch area to heal. This experiment simulates the migration process of cell healing in vivo.
[0003] The cell scratch test is usually carried out in a culture dish. The culture dish in the related art includes a base and a cover. The cells are located in the culture wells of the base, and the cover is set on the base to put the cells in a relatively closed environment. During the experiment, it is necessary to take pictures of the cells with a microscope at multiple time points, such as 0 hours, 24 hours, 48 hours, 72 hours and 96 hours after the scratch, to record the process of cell migration.
[0004] The culture dish (especially the upper cover) in the related art is usually made of transparent plastic material. If the cells are directly observed through the plastic using a microscope, a clear image cannot be obtained. Therefore, the upper cover needs to be opened to take pictures. However, opening the upper cover to take pictures has the following problems:
[0005] First, since each photo shoot takes a long time, it is easy to destroy the sterile environment in the culture dish, increasing the risk of cell contamination;
[0006] Secondly, after opening the upper cover, the temperature, humidity and gas composition of the environment in the culture dish are affected, which may affect the growth state of the cells and reduce the accuracy of the experimental results;
[0007] Thirdly, the photo-taking process is easily affected by external lighting conditions, resulting in unstable image quality;
[0008] Fourthly, the removal and installation of the upper cover increases the burden on the operators. Utility Model Content
[0009] The present invention provides a cell scratch experiment photography culture dish that does not require opening of the cover, which can be used to photograph cells without opening the upper cover.
[0010] The cell scratch experiment photography-taking culture dish provided by the present disclosure includes:
[0011] a base, the base comprising a culture hole with an upper opening, the culture hole comprising a bottom wall; and
[0012] An upper cover, which can be covered on the base, and includes a top wall. When the upper cover is covered on the base, the top wall corresponds to the culture hole;
[0013] Wherein, the bottom wall and the top wall are both made of transparent glass.
[0014] Compared with the prior art, the cell scratch experiment photography-taking culture dish provided by the present disclosure has at least the following beneficial effects:
[0015] The material of the bottom wall of the culture hole in the base is transparent glass, and the material of the top wall corresponding to the culture hole in the upper cover is also transparent glass. Compared with plastic, the influence of transparent glass on the image quality under a microscope is significantly reduced. Therefore, according to the cell scratch experiment photography culture dish without opening the cover disclosed in the present invention, cells can be photographed without opening the upper cover.
[0016] Since there is no need to open the upper cover, the risk of cell contamination can be reduced, the impact on the growth state of the cells can be reduced, the impact of external light conditions during the photographing process can be reduced, and the burden on the operator can be reduced. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 A schematic diagram of the base and the upper cover viewed obliquely from above provided by an embodiment of the present disclosure is shown.
[0018] Figure 2 A schematic diagram of the base and the upper cover provided by an embodiment of the present disclosure observed from an oblique downward direction is shown.
[0019] Figure 3 A schematic diagram of a culture dish for observing a cell scratch experiment and taking photos from above without opening the lid provided in an embodiment of the present disclosure is shown.
[0020] Figure 4 A schematic diagram of a culture dish for observing a cell scratch experiment and taking photos from below without opening the lid provided in an embodiment of the present disclosure is shown.
[0021] Figure 5 A schematic diagram showing two culture dishes for cell scratch experiment photography without opening the lid provided in an embodiment of the present disclosure stacked up one above the other is shown.
[0022] Figure 6 An exploded view of the baffle provided by an embodiment of the present disclosure and a culture dish for taking photos of a cell scratch experiment without opening the lid is shown.
[0023] Figure 7 A schematic diagram showing a baffle provided by an embodiment of the present disclosure is installed between two culture dishes stacked up and down for taking photos of cell scratch experiments without opening the lid.
[0024] Figure 8 Shows Figure 7 A magnified view of the part within the dotted box.
[0025] Reference numerals:
[0026] 1. base; 11. culture hole; 111. side wall; 112. bottom wall; 12. lower shell; 121. first groove; 122. second protrusion; 123. support member;
[0027] 2. Upper cover; 21. Top wall; 22. Upper shell; 221. First protrusion; 222. Second groove; 223. Guide groove;
[0028] 3. Baffle. DETAILED DESCRIPTION
[0029] The technical solution of the present disclosure is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the present disclosure, rather than to limit the present disclosure. It should also be noted that, for ease of description, only the parts related to the present disclosure are shown in the accompanying drawings, rather than all of them.
[0030] Some directional words are defined in the present disclosure. Unless otherwise stated, the directional words used, such as "up", "down", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of the present disclosure.
[0031] In the present disclosure, unless otherwise expressly specified and limited, a first feature being “above” or “below” a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being “above”, “above”, and “above” a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.
[0032] In the description of the present disclosure, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present disclosure can be understood according to specific circumstances.
[0033] The disclosed embodiments provide a culture dish for taking photos of cell scratch experiments without opening the lid (hereinafter referred to as the culture dish). The culture dish can at least be used in cell scratch experiments, but is not limited to this, and can also be used in experiments that require cell culture, such as photochemical experiments.
[0034] Figure 1 and Figure 2 Schematic diagram of a culture dish provided by an embodiment of the present disclosure is shown. Figure 1 and Figure 2 As shown, the culture dish includes a base 1 and an upper cover 2 which can be separated from each other. The base 1 includes one or more culture wells 11, which are holes extending downward from the upper surface of the base 1 to a certain depth, that is, the culture well 11 has an opening toward the upper end, and the culture well 11 is not a through hole, but a countersunk hole with a bottom wall 112, through which cells can be added to the bottom wall 112 of the culture well 11. When the upper cover 2 is covered on the base 1, a relatively closed space is formed between the upper cover 2 and the base 1, especially in the culture well 11, so as to reduce the influence of the external environment on the cells in the culture well 11.
[0035] It should be noted that the shape of the base 1 is not limited and can be Figure 1 The structure shown is generally rectangular, but it may also be a circular structure or other shapes. Similarly, the shape of the upper cover 2 is not limited.
[0036] It should be noted that the shape of the culture hole 11 is not limited and can be Figure 1 The shape shown is roughly cylindrical, but it can also be a polygon such as a rectangle, or a combination of a polygon and a circle.
[0037] It should be noted that when there are multiple culture wells 11, the arrangement of the culture wells 11 is not limited and can be as follows: Figure 1 The arrangement shown is a rectangular array, but it can also be arranged in a circular array or other regular / irregular arrangements.
[0038] like Figure 1 and Figure 2 As shown, when the upper cover 2 is covered on the base 1, a portion of the upper cover 2 corresponds to the culture hole 11. Specifically, a portion of the upper cover 2 is located at the opening of the culture hole 11, or below the opening, or above the opening, and this portion of the upper cover 2 is defined as the top wall 21. Therefore, when the upper cover 2 is covered on the base 1, the top wall 21 corresponds to the culture hole 11.
[0039] It should be noted that in the embodiment of the present disclosure, the bottom wall 112 and the top wall 21 are made of transparent glass. Compared with the culture dish in the prior art in which one or both of the bottom wall 112 and the top wall 21 are made of plastic, the culture dish in the embodiment of the present disclosure can take pictures of the cells in the culture well 11 using a microscope without opening the upper cover 2, and still ensure that the obtained image has good clarity, so as to ensure the accuracy of the experimental results.
[0040] In addition, since there is no need to open the upper cover 2, the risk of cell contamination can be reduced, the impact on the growth state of the cells can be reduced, the impact of external lighting conditions during the photographing process can be reduced, and the burden on the operator can be reduced.
[0041] It should be noted that the size of the top wall 21 is usually greater than or equal to the size of the opening of the culture well 11, so that cells at various positions in the culture well 11 can be observed when taking pictures using a microscope.
[0042] like Figure 1 and Figure 2 As shown, the base 1 may further include a lower shell 12, which refers to the rest of the base 1 except the bottom wall 112, and the lower shell 12 includes one or more first through holes, which penetrate the upper surface and the lower surface of the lower shell 12, and the bottom wall 112 is connected in the first through holes, so that the part of the first through hole located above the bottom wall 112 forms the culture hole 11. The side wall 111 of the culture hole 11 is defined by the lower shell 12.
[0043] Specifically, in Figure 2 In the embodiment, the bottom wall 112 is connected to the bottom of the first through hole, so the entire first through hole can be considered as the culture hole 11. However, in an embodiment not shown, the bottom wall 112 can also be connected to the middle of the first through hole, so that only the upper part of the first through hole is the culture hole 11, and the lower part of the first through hole is not the culture hole 11.
[0044] It should be noted that the material of the lower housing 12 can be plastic or glass. For ease of use, plastic is preferred because glass is easily broken.
[0045] It should be noted that the lower surface of the bottom wall 112 can be higher than the lower surface of the lower shell 12, thereby forming a height difference between the bottom wall 112 and the lower shell 12. When the base 1 is placed on a fixed surface such as a desktop, the lower surface of the bottom wall 112 will not directly contact the fixed surface, thereby avoiding friction between the bottom wall 112 and the fixed surface, resulting in scratches on the lower surface of the bottom wall 112, thereby affecting the clarity of the image obtained by taking a photo.
[0046] As a first implementation manner, the bottom wall 112 may be disposed in the middle of the first through hole as described above, so that the lower surface of the bottom wall 112 is higher than the lower surface of the lower shell 12 .
[0047] As a second implementation method, Figure 3 and Figure 4 As shown, the lower shell 12 may further include a second protrusion 122 and / or a support member 123 arranged at the bottom of the base 1, whereby, even if the bottom wall 112 is located at the bottom of the first through hole, the lower surface of the base 1, that is, the lower surface of the second protrusion 122 or the lower surface of the support member 123 is lower than the lower surface of the bottom wall 112.
[0048] like Figure 1 and Figure 2 As shown, the upper cover 2 may further include an upper shell 22, which refers to the remaining portion of the upper cover 2 except the top wall 21. The upper shell 22 may include one or more second through holes, which pass through the upper and lower surfaces of the upper shell 22, and the top wall 21 is connected to the second through holes.
[0049] The thickness of the top wall 21 may be consistent with the depth of the second through hole, or may be smaller than the depth of the second through hole.
[0050] It should be noted that the material of the upper housing 22 can be plastic or glass. For ease of use, plastic is preferred because glass is easily broken.
[0051] It should be noted that the upper surface of the top wall 21 can be lower than the upper surface of the upper shell 22, thereby forming a height difference between the top wall 21 and the upper shell 22. When multiple culture dishes are stacked on each other, the upper surface of the top wall 21 will not directly contact the lower shell 12 or the bottom wall 112 of the culture dish above, thereby avoiding friction on the top wall 21, which will cause scratches on the upper surface of the top wall 21, thereby affecting the clarity of the image obtained by taking a photo.
[0052] As a first implementation manner, the top wall 21 is disposed in the middle of the second through hole, so that the upper surface of the top wall 21 is lower than the upper surface of the upper shell 22 .
[0053] As a second implementation method, Figure 3 As shown, the upper shell 22 can further include a first protrusion 221 arranged on the top of the upper cover 2, whereby, even if the top wall 21 is located at the top of the second through hole, the upper surface of the upper cover 2, that is, the upper surface of the first protrusion 221 is higher than the upper surface of the top wall 21.
[0054] Optionally, a first protective film (not shown) is detachably provided on the lower surface of the bottom wall 112, and a second protective film (not shown) is detachably provided on the upper surface of the top wall 21. Thus, before the culture dish is put into use, the glass can be protected by the protective film to avoid scratches on the glass.
[0055] It should be noted that the protective film can be any protective film known in the prior art, especially a protective film that does not leave traces after separation, such as a self-adhesive film.
[0056] Figure 5 FIG. 2 shows a schematic diagram of two culture dishes stacked up and down provided by an embodiment of the present disclosure. Figure 5 Shown and combined Figure 3 and Figure 4 The base 1 may further include a first groove 121, and the upper cover 2 may further include a first protrusion 221 corresponding to the first groove 121. The first protrusion 221 of the culture dish located below may be inserted into the first groove 121 of the culture dish located above, so as to facilitate stacking of multiple culture dishes and ensure that the multiple culture dishes have two stability after being stacked and will not fall over easily.
[0057] In addition, the base 1 may further include a second protrusion 122, and the upper cover 2 may further include a second groove 222 corresponding to the second protrusion 122, so that the second protrusion 122 of the culture dish located below can be inserted into the second groove 222 of the culture dish located above, so that multiple culture dishes can be stacked, and it is ensured that the multiple culture dishes have good stability after being stacked and will not fall over easily. So that multiple culture dishes can be stacked.
[0058] It should be noted that the first protrusion 221 and the first groove 121 , and the second protrusion 122 and the second groove 222 may be provided separately or simultaneously.
[0059] In this embodiment, the second protrusion 122 can be considered as the inner region surrounded by the first groove 121. The second groove 222 can be considered as the inner region of the first protrusion 221.
[0060] like Figure 6 As shown, the culture dish may further include a baffle 3, and the upper cover 2 may further include a guide groove 223 for the baffle 3 to be laterally inserted, and the height of the guide groove 223 is higher than the top wall 21. When the baffle 3 is located in the guide groove 223, the baffle 3 covers the top wall 21 from above, so that when the upper and lower culture dishes are stacked, the baffle 3 separates the bottom of the culture dish located above (especially the bottom wall 112) from the top of the culture dish located below (especially the top wall 21) to avoid friction of the top wall 21 and / or the bottom wall 112.
[0061] like Figure 7 and Figure 8 As shown, the base 1 may further include a support member 123 located at the bottom of the base 1. When multiple culture dishes are stacked on each other, the lower surface of the support member 123 of the upper culture dish abuts against the upper surface of the baffle 3, making the connection between the upper and lower culture dishes more stable.
[0062] Although the present disclosure has been described in detail above by means of general description, specific implementation methods and experiments, it is obvious to those skilled in the art that some modifications or improvements may be made to the present disclosure. Therefore, these modifications or improvements made without departing from the spirit of the present disclosure are within the scope of protection claimed by the present disclosure.
Claims
1. A cell scratch experiment photography culture dish without opening the lid, characterized in that: The cell scratch test photography-taking culture dish without opening the cover comprises: A base (1), the base (1) comprising a culture hole (11) with an upper opening, the culture hole (11) comprising a bottom wall (112); and An upper cover (2), the upper cover (2) can be covered on the base (1), the upper cover (2) comprises a top wall (21), and when the upper cover (2) is covered on the base (1), the top wall (21) corresponds to the culture hole (11); Wherein, the bottom wall (112) and the top wall (21) are both made of transparent glass.
2. The cell scratch test photography-free culture dish according to claim 1, characterized in that: The base (1) further comprises: A lower shell (12), wherein the lower shell (12) comprises a first through hole, and the bottom wall (112) is connected to the first through hole so that a portion of the first through hole located above the bottom wall (112) forms the culture hole (11).
3. The cell scratch test photography-free culture dish according to claim 2, characterized in that: The lower shell (12) is made of plastic; and / or The lower surface of the bottom wall (112) is higher than the lower surface of the lower shell (12).
4. The cell scratch test photography-free culture dish according to claim 1, characterized in that: The upper cover (2) further comprises: An upper shell (22), wherein the upper shell (22) comprises a second through hole, and the top wall (21) is connected in the second through hole.
5. The cell scratch test photography-free culture dish according to claim 4, characterized in that: The upper shell (22) is made of plastic; and / or The upper surface of the top wall (21) is lower than the upper surface of the upper shell (22).
6. The cell scratch test photography culture dish without opening the cover according to any one of claims 1 to 5, characterized in that: A first protective film is detachably provided on the lower surface of the bottom wall (112).
7. The cell scratch test photography culture dish without opening the cover according to any one of claims 1 to 5, characterized in that: A second protective film is detachably provided on the upper surface of the top wall (21).
8. The cell scratch test photography culture dish without opening the cover according to any one of claims 1 to 5, characterized in that: The base (1) further comprises a groove, and the upper cover (2) further comprises a protrusion corresponding to the groove, so that the protrusion of one cell scratch experiment photography culture dish without opening the cover can be inserted into the groove of another cell scratch experiment photography culture dish without opening the cover; or The base (1) further comprises a protrusion, and the upper cover (2) further comprises a groove corresponding to the protrusion, so that the protrusion of one cell scratch experiment photography culture dish without opening the cover can be inserted into the groove of another cell scratch experiment photography culture dish without opening the cover.
9. The cell scratch test photography-free culture dish according to claim 8, characterized in that: The cell scratch experiment photography culture dish without opening the cover further comprises a baffle (3), and the upper cover (2) further comprises a guide groove (223) for the baffle (3) to be inserted laterally, the height of the guide groove (223) is higher than the top wall (21), and when the baffle (3) is located in the guide groove (223), the baffle (3) covers the top wall (21) from above.
10. The cell scratch test photography-free culture dish according to claim 9, characterized in that: The base (1) further comprises a support member (123) located at the bottom of the base (1); when a plurality of the cell scratch experiment photography cover-free culture dishes are stacked on each other, the lower surface of the support member (123) abuts against the upper surface of the baffle (3).