Heating device of full-automatic nucleic acid extractor

Abstract

The utility model discloses a full-automatic nucleic acid extractor heating device, which relates to the technical field of nucleic acid extractors and comprises a first frame body, a partition plate is vertically and fixedly connected to the bottom wall in the first frame body, and a plurality of first rotating rods are horizontally and rotatably connected between the partition plate and the first frame body. A plurality of gears are fixedly connected to the outer side of the first rotating rod; a second frame body is placed at the position, located on the upper side of the gear, in the first frame body, a plurality of heating assemblies are fixedly installed on the bottom wall in the second frame body, each heating assembly comprises a power source base, a power source button is arranged in the center of the top of each power source base, and fixing rods are evenly and fixedly connected to the edge of the top of each power source base; the other ends of the fixing rods are fixedly connected with the same sliding sleeve. The sliding sleeve slides and is sleeved with a heating sleeve in a clamping manner; the problems that an existing heating device of the nucleic acid extractor cannot actively digest and heat to generate bubbles and a heating sleeve is folded in use are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extractors, in particular to a fully automatic nucleic acid extractor heating device. Background Art

[0002] The nucleic acid extractor is an instrument that uses matching nucleic acid extraction reagents to automatically complete the sample nucleic acid extraction work. It is widely used in various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry and molecular biology research.

[0003] The Chinese utility model with publication number CN218561423U proposes a heating device for a nucleic acid extractor, which relates to the technical field of nucleic acid extractors. The beneficial effects of this case are: it solves the problem that the existing nucleic acid extractor needs to be heated at different temperatures for different detection objects when in use to increase the release rate of the substance. The heating structure of the nucleic acid extractor is simple, and it is mostly heated from the side through a heating plate. The heating effect is uneven, and the heating speed is very slow, the use efficiency is low, and the detection speed is affected.

[0004] However, the above practicality still has shortcomings:

[0005] 1. The utility model adopts a wrapping heating method, which is conducive to uniform heating of the nucleic acid reagent tube, but tiny bubbles will be generated during the heating process. The cleavage reaction usually lasts for several minutes to several hours at a temperature of 55 to 65 degrees, which will continue to generate a large number of bubbles. The bubbles act as an obstacle to heat transfer, resulting in uneven heating in local areas, which in turn affects the uneven cleavage reaction rate in the test tube. The utility model does not set a structure to dissolve bubbles, so the accumulation of bubbles has a negative impact on the experimental results.

[0006] 2. When the heating sleeve in the utility model is actually used, there is a risk that when the reagent tube is inserted into the heating sleeve, it will abut against the top of the heating sleeve, causing the heating sleeve to fold, thereby affecting the service life of the heating sleeve. Utility Model Content

[0007] The utility model aims to solve at least one of the technical problems existing in the prior art. To this end, the utility model proposes a fully automatic nucleic acid extractor heating device, which solves the problem that the existing nucleic acid extractor heating device cannot actively eliminate bubbles generated by heating and the heating sleeve folds during use.

[0008] In order to achieve the above-mentioned purpose, the utility model proposes a heating device for a fully automatic nucleic acid extractor.

[0009] As a further solution of the utility model:

[0010] A fully automatic nucleic acid extractor heating device, including a first housing, a partition is vertically and fixedly connected to the inner bottom wall of the first housing, and a plurality of first rotating rods are horizontally rotatably connected between the partition and the first housing, and a plurality of gears are fixedly connected to the outside of the first rotating rods;

[0011] A second housing is placed above the gears inside the first housing. A plurality of heating components are fixedly installed on the inner bottom wall of the second housing. Each heating component includes a power supply base, a power button is arranged at the center of the top of the power supply base, and fixing rods are evenly and fixedly connected to the top edge of the power supply base. The other ends of the fixing rods are all fixedly connected to the same sliding sleeve; the sliding sleeve slides and is snap-fitted with a heating sleeve;

[0012] The top end of the heating sleeve is fixedly connected with a sleeve plate, and first lead screws are vertically threadedly connected to the four corners of the sleeve plate. The first lead screws are rotatably connected to the second housing;

[0013] A second lead screw is rotatably connected to the second housing, and a reagent plate is threadedly connected to the second lead screw.

[0014] Furthermore, a plurality of first motors and second motors are respectively fixedly connected to the top of the side wall of the second housing. The output ends of the first motors are all fixedly connected to the first lead screws, and the output ends of the second motors are all fixedly connected to the second lead screws.

[0015] Furthermore, a plurality of reagent tube holes for snap-fitting reagent tubes are arranged on the surface of the reagent plate.

[0016] Furthermore, both the first housing and the second housing are housings with open tops.

[0017] Furthermore, the inner ends of the first rotating rods all pass through the partition and are fixedly connected with second bevel gears. A plurality of second rotating rods are rotatably connected to the inner bottom wall of the first housing. The tops of the second rotating rods are all fixedly connected with first bevel gears. The tops of the first bevel gears are all fixedly connected with sleeves. Belts are respectively sleeved between the sleeves and are connected by the belts; the tops of some of the sleeves are fixedly connected with third motors.

[0018] Furthermore, a cover plate is rotatably connected to the top of the first housing, and the cover plate controls the opening and closing of the top of the second housing.

[0019] Furthermore, a heating mesh is arranged inside the heating sleeve. The power button controls the connection and disconnection of the circuit of the heating sleeve. The heating sleeve is connected to the power supply base by wire control.

[0020] Further, the maximum sliding distance between the sliding sleeve and the heating sleeve is equal to the distance between the bottom of the heating sleeve and the power button.

[0021] Further, a plurality of springs are fixedly connected to the inner end of the cover plate, and the inner ends of the springs are fixedly connected to the same pressing plate.

[0022] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0023] The present utility model realizes uniform heating of the nucleic acid reagent tube, thereby improving the separation speed of nucleic acid; at the same time, it avoids the problems of easy folding of the heating sleeve and internal heating and foaming of the reagent tube in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a perspective view of the present utility model;

[0025] Figure 2 is a schematic view of the internal structure of the first frame of the present utility model Figure 1 ;

[0026] Figure 3 is a schematic view of the internal structure of the first frame of the present utility model Figure 2 ;

[0027] Figure 4 is a schematic view of the internal structure of the second frame of the present utility model;

[0028] Figure 5 is a schematic view of the structure of the heating component of the present utility model.

[0029] In the figure: 1, the first frame; 2, the second frame; 3, the cover plate; 4, the partition; 5, the first rotating rod; 6, the gear; 7, the sleeve plate; 8, the reagent plate; 81, the reagent tube hole; 9, the first motor; 10, the second motor; 11, the first lead screw; 12, the second lead screw; 13, the heating component; 131, the heating sleeve; 132, the sliding sleeve; 133, the fixed rod; 134, the power supply base; 135, the power button; 14, the second rotating rod; 15, the first bevel gear; 16, the belt; 18, the sleeve; 19, the second bevel gear; 20, the third motor. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] Hereinafter, the technical solutions of the present utility model will be clearly and completely described in conjunction with the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0031] As Figures 1-5As shown, a fully automatic nucleic acid extraction device heating device comprises a first frame 1, a partition 4 is vertically fixedly connected to the bottom wall inside the first frame 1, a plurality of first rotating rods 5 are horizontally rotatably connected between the partition 4 and the first frame 1, and a plurality of gears 6 are fixedly connected to the outer side of the first rotating rod 5;

[0032] A second frame 2 is placed on the upper side of the gear 6 inside the first frame 1, and a plurality of heating components 13 are fixedly installed on the bottom wall inside the second frame 2. The heating components 13 include a power base 134, and a power button 135 is arranged at the top center of the power base 134. A fixing rod 133 is evenly fixedly connected to the top edge of the power base 134, and the other end of the fixing rod 133 is fixedly connected to the same sliding sleeve 132; the sliding sleeve 132 slides and is clamped with a heating sleeve 131;

[0033] The top of the heating sleeve 131 is fixedly connected with a sleeve plate 7, and the four corners of the sleeve plate 7 are vertically threadedly connected with a first screw rod 11, and the first screw rod 11 is rotatably connected to the second frame 2;

[0034] The second frame 2 is rotatably connected to a second screw rod 12 , and the second screw rod 12 is threadably connected to a reagent plate 8 .

[0035] Preferably, a plurality of first motors 9 and second motors 10 are fixedly connected to the top of the side walls of the second frame 2 , respectively; the output ends of the first motors 9 are fixedly connected to the first screw rod 11 , and the output ends of the second motors 10 are fixedly connected to the second screw rod 12 .

[0036] Preferably, the surface of the reagent plate 8 is provided with a plurality of reagent tube holes 81 for clamping reagent tubes.

[0037] Preferably, the first frame body 1 and the second frame body 2 are both frames with top openings.

[0038] Preferably, the inner ends of the first rotating rods 5 pass through the partition 4 and are fixedly connected to the second bevel gear 19, the bottom wall inside the first frame 1 is rotatably connected to a plurality of second rotating rods 14, the tops of the second rotating rods 14 are fixedly connected to the first bevel gears 15, the tops of the first bevel gears 15 are fixedly connected to sleeves 18, belts 16 are respectively sleeved between the sleeves 18 and are connected through the belts 16; the tops of some of the sleeves 18 are fixedly connected to third motors 20.

[0039] Preferably, a cover plate 3 is rotatably connected to the top of the first frame body 1 , and the cover plate 3 controls the opening and closing of the top of the second frame body 2 .

[0040] Preferably, a heating net is provided inside the heating sleeve 131. The power button 135 controls the connection and disconnection of the circuit of the heating sleeve 131. The heating sleeve 131 is connected to the power base 134 through wire control.

[0041] Preferably, the maximum sliding distance between the sliding sleeve 132 and the heating sleeve 131 is equal to the distance between the bottom of the heating sleeve 131 and the power button 135.

[0042] Preferably, several springs are fixedly connected to the inner end of the cover plate 3, and the inner ends of the springs are all fixedly connected to the same pressure plate.

[0043] Working principle:

[0044] During use, insert the reagent tube into the reagent tube hole 81 at the top of the reagent plate 8. This embodiment preferably uses a nucleic acid analyzer with a 4*6 specification; after inserting the reagent tube, close the cover plate 3; then the first motor 9 drives the first lead screw 11 to rotate, so that the sleeve plate 7 moves upward; the second motor 10 drives the second lead screw 12 to rotate, so that the reagent plate 8 moves downward; the pressure plate at the inner end of the cover plate 3 keeps the reagent tube always relatively fixed with respect to the reagent plate 8, so that the reagent tube is inserted into the heating sleeve 131; it avoids folding the heating sleeve 131 when inserting it into the heating sleeve 131 and ensures the stability of the insertion of the reagent tube. When the reagent tube is completely inserted into the heating sleeve 131, the reagent tube continues to move downward, so that relative sliding occurs between the sliding sleeve 132 and the heating sleeve 131, causing the bottom of the heating sleeve 131 to press the power button 135, and then driving the power base 134 to supply power to the heating sleeve 131, thereby uniformly heating the reagent tube;

[0045] When there is no reagent tube inserted into the reagent tube hole 81, the heating sleeve 131 is made of a flexible material. When the bottom of the heating sleeve 131 touches the power button 135, there is no downward pressing, thus avoiding ineffective power waste and at the same time avoiding the risk of dry burning of the heating sleeve 131;

[0046] When heating the reagent tube, fine bubbles will be generated in the reagent tube wall. The third motor 20 is fixed on the partition plate 4. The third motor 20 drives the sleeve 18 to rotate, and then drives all the sleeves 18 to rotate through the belt 16, causing the first bevel gear 15 to rotate, and then driving the second bevel gear 19 to rotate, so that the first rotating rod 5 rotates, so that the gear 6 rotates. While the gear 6 rotates, it generates a slight vibration of the second frame body 2, thereby eliminating the bubbles in the reagent tube;

[0047] The utility model realizes uniform heating of the nucleic acid reagent tube, thereby improving the separation speed of nucleic acid; at the same time, it avoids the problems that the heating sleeve 131 is prone to folding and the internal heating of the reagent tube generates bubbles in the prior art;

[0048] The above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A heating device for a fully automatic nucleic acid extractor, characterized in that: It comprises a first frame (1), a partition (4) is vertically fixedly connected to the bottom wall inside the first frame (1), a plurality of first rotating rods (5) are horizontally rotatably connected between the partition (4) and the first frame (1), and a plurality of gears (6) are fixedly connected to the outer side of the first rotating rod (5); A second frame (2) is placed inside the first frame (1) on the upper side of the gear (6); a plurality of heating components (13) are fixedly mounted on the bottom wall inside the second frame (2); the heating components (13) include a power base (134); a power button (135) is arranged at the top center of the power base (134); a fixing rod (133) is evenly fixedly connected to the top edge of the power base (134); the other end of the fixing rod (133) is fixedly connected to the same sliding sleeve (132); the sliding sleeve (132) is slidably and snap-fitted with a heating sleeve (131); The top end of the heating sleeve (131) is fixedly connected to a sleeve plate (7), and the four corners of the sleeve plate (7) are vertically threadedly connected to a first screw rod (11), and the first screw rod (11) is rotatably connected to the second frame (2); The second frame (2) is rotatably connected to a second screw rod (12), and the second screw rod (12) is threadably connected to a reagent plate (8).

2. A heating device for a fully automatic nucleic acid extractor according to claim 1, characterized in that: A plurality of first motors (9) and second motors (10) are respectively fixedly connected to the top of the side wall of the second frame body (2); the output ends of the first motors (9) are fixedly connected to the first screw rod (11), and the output ends of the second motors (10) are fixedly connected to the second screw rod (12).

3. A heating device for a fully automatic nucleic acid extractor according to claim 2, characterized in that: The surface of the reagent plate (8) is provided with a plurality of reagent tube holes (81) for clamping reagent tubes.

4. A heating device for a fully automatic nucleic acid extractor according to claim 3, characterized in that: The first frame (1) and the second frame (2) are both frames with an open top.

5. A heating device for a fully automatic nucleic acid extractor according to claim 4, characterized in that: The inner ends of the first rotating rods (5) pass through the partition (4) and are fixedly connected to the second bevel gear (19); the bottom wall inside the first frame (1) is rotatably connected to a plurality of second rotating rods (14); the tops of the second rotating rods (14) are fixedly connected to the first bevel gear (15); the tops of the first bevel gears (15) are fixedly connected to sleeves (18); belts (16) are respectively sleeved between the sleeves (18) and are connected through transmission by the belts (16); the tops of some of the sleeves (18) are fixedly connected to third motors (20).

6. A heating device for a fully automatic nucleic acid extractor according to claim 5, characterized in that: The top of the first frame body (1) is rotatably connected to a cover plate (3), and the cover plate (3) controls the opening and closing of the top of the second frame body (2).

7. A heating device for a fully automatic nucleic acid extractor according to claim 6, characterized in that: The heating sleeve (131) is provided with a heating network inside, and the power button (135) controls the connection and disconnection of the circuit of the heating sleeve (131), and the heating sleeve (131) is connected to the power base (134) via a wire control.

8. A heating device for a fully automatic nucleic acid extractor according to claim 7, characterized in that: The maximum sliding distance between the sliding sleeve (132) and the heating sleeve (131) is equal to the distance between the bottom of the heating sleeve (131) and the power button (135).

9. A heating device for a fully automatic nucleic acid extractor according to claim 8, characterized in that: The inner end of the cover plate (3) is fixedly connected to a plurality of springs, and the inner ends of the springs are all fixedly connected to the same pressing plate.

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