Cell recovery device

Abstract

The utility model relates to the technical field of cell water bath resuscitation, in particular to a cell resuscitation device which comprises a floating plate and a resuscitation bag, a groove allowing the resuscitation bag to be clamped is formed in the plate surface of the floating plate in a penetrating mode, and the resuscitation bag comprises a sealing part and a plurality of warm bath sleeves arranged at the lower end of the sealing part at intervals; a cryopreservation tube is inserted into the warm bath sleeve, and a clamping chain is arranged at the upper end of the sealing part. In the cell resuscitation process, the cryopreservation tube is stored in the resuscitation bag, inclination and collision of the cryopreservation tube are limited through the warm bath sleeve at the fixed position, and hot water is prevented from making direct contact with a tube cover of the cryopreservation tube through the sealed resuscitation bag, so that the probability that cells are polluted in the cell resuscitation process is effectively reduced, and the sterility of the cell resuscitation heating process is ensured.

Description

Technical Field

[0001] This application relates to the technical field of cell water bath resuscitation, and particularly to a cell resuscitation device. Background Art

[0002] In the laboratory, cells need to be cryopreserved to maintain their long-term viability and function. When conducting experiments or production, these cryopreserved cells need to be resuscitated to restore them to an active physiological state.

[0003] Cell resuscitation refers to the process of restoring cells in a cryopreserved state to an active state through appropriate methods. This process usually requires quickly transferring the cells in the cryotube from a low-temperature environment to a suitable growth temperature to ensure the survival rate and functional integrity of the cells. The commonly used method is to place the cryotube containing the cryopreserved cells in a constant-temperature water bath for heating to quickly thaw the cells.

[0004] In traditional cell resuscitation methods, laboratories generally place the cryotube on a float or directly clamp the cryotube with a hemostat and heat it in hot water. Although these methods are simple, they greatly increase the risk of direct contact between the hot water in the water bath and the cryotube cap. In addition, since multiple cryotubes usually need to be resuscitated simultaneously during the experiment, when using a float for fixation, multiple cryotubes are prone to tilting or squeezing together, further increasing the chance of the tube cap becoming loose and hot water entering. During the resuscitation process, once hot water enters the cryotube, it will cause cell contamination, and the experimenter needs to re-culture the cells, which greatly wastes manpower and material resources. Utility Model Content

[0005] In order to reduce the probability of cell contamination during cell resuscitation and ensure the sterility of the cell resuscitation heating process, this application provides a cell resuscitation device.

[0006] A cell resuscitation device provided by this application adopts the following technical solution:

[0007] A cell resuscitation device includes a floating board and a resuscitation bag. A groove for clamping the resuscitation bag is formed through the plate surface of the floating board. The resuscitation bag includes a sealing part and a plurality of temperature bath sleeves arranged at intervals below the sealing part. The temperature bath sleeves are for inserting cryotubes, and a zipper is arranged at the upper end of the sealing part.

[0008] By adopting the above technical solution, during cell resuscitation, the cryopreservation tubes can be inserted into the warm bath sleeves one by one. The warm bath sleeves are connected to the sealing part, reducing the probability of the cryopreservation tubes tilting and colliding. Subsequently, the closing clip chain is pressed with fingers, and the heat-sealed part is heat-sealed by thermoplastic, achieving double sealing. This device can not only effectively prevent the water in the water bath from contacting the cryopreservation tubes, thereby reducing the probability of cell contamination during cell resuscitation, but also ensure that the cryopreservation tubes are fully and separately in contact with water, avoiding poor resuscitation effects caused by uneven heating of the cryopreservation tubes, thus improving the resuscitation efficiency.

[0009] Optionally, a heat-sealed part is provided above the clip chain on the resuscitation bag.

[0010] By adopting the above technical solution, a heat-sealed part is added on the basis of the clip chain. After inserting the cryopreservation tubes, secondary sealing is performed. During the water bath process, the heat-sealed part can ensure that no water enters the bag, avoiding contamination problems caused by incomplete sealing of the clip chain.

[0011] Optionally, the resuscitation bag is a PE bag.

[0012] By adopting the above technical solution, the PE material has good waterproofness and durability, and low cost, meeting the market demand.

[0013] Optionally, the warm bath sleeve is a strip-shaped bag.

[0014] By adopting the above technical solution, the design of the strip-shaped bag matches the strip design of the cryopreservation tube, avoiding the cryopreservation tube from tilting in the warm bath sleeve and ensuring that all parts of the cryopreservation tube are evenly in contact with hot water, thus avoiding uneven heating of the cryopreservation tube.

[0015] In summary, the beneficial effects of this application are as follows: The resuscitation bag of this application is made of PE material, with excellent performance, low price, and extremely low replacement cost, meeting the requirements of biological experiments. In addition, the resuscitation bag has a clip chain that can be automatically sealed by finger pressure, a heat-sealed part that needs to be heat-sealed after being torn, and a warm bath sleeve for storing cryopreservation tubes. The warm bath sleeve is a strip-shaped bag, and each strip-shaped bag holds one cryopreservation tube. Generally speaking, it can effectively avoid the cells in the cryopreservation tube from being contaminated by water during water bath resuscitation and also avoid uneven heating of the cryopreservation tube. Description of the Drawings

[0016] Figure 1 It is a schematic structural diagram of the resuscitation bag in the embodiment of this application.

[0017] Figure 2 It is a schematic structural diagram of the floating board in the embodiment of this application.

[0018] Description of the reference numerals: 1. Floating board; 11. Groove; 2. Resuscitation bag; 21. Sealing part; 211. Clip chain; 212. Heat-sealed part; 22. Warm bath sleeve. Detailed implementation manners

[0019] The following further elaborates on this application in conjunction with the Figure 1-2 accompanying drawings.

[0020] An embodiment of this application discloses a cell recovery device. Referring to Figure 1 and Figure 2 , the cell recovery device includes a floating board 1 and a recovery bag 2. A groove 11 for snap-fitting the recovery bag 2 is formed through the plate surface of the floating board 1. The floating board 1 is used to limit the immersion of the recovery bag 2 in water. Based on the requirements of cost and performance, the recovery bag 2 is made of PE material, which has good waterproofness and durability, and is low in cost and can be replaced after use.

[0021] The recovery bag 2 includes an integrally formed sealing part 21 and a warm bath sleeve 22. A plurality of warm bath sleeves 22 are arranged at intervals along the width direction of the recovery bag 2 at the lower end of the sealing part 21. In this embodiment of the application, it is illustrated by taking the same spacing between adjacent warm bath sleeves 22 as an example.

[0022] The upper end of the warm bath sleeve 22 is communicated with the sealing part 21, and the upper end of the sealing part 21 is communicated with the outside. The cryopreservation tube can be inserted into the warm bath sleeve 22 from top to bottom. The warm bath sleeve 22 is a strip-shaped bag matching the shape of the cryopreservation tube, similar to the shape of a finger, and the warm bath sleeve 22 corresponds to the strip-shaped bag one by one. In actual production, the recovery bag 2 with different numbers of warm bath sleeves 22 can be selected for production according to specific requirements, and it is ensured that the width of the floating board 1 matches that of the recovery bag 2. This embodiment of the application is illustrated by taking a total of five warm bath sleeves 22 as an example.

[0023] A zipper 211 is formed on the sealing part 21 above the warm bath sleeve 22. The zipper 211 can be sealed by finger pressure. There is a certain distance between the zipper 211 and the top of the sealing part 21, so that the zipper 211 is protected inside the sealing part 21, thereby reducing the probability of the zipper 211 contacting water and reducing the probability of the cryopreservation tube being contaminated. In a specific implementation manner, only the zipper 11 is used for sealing; in another specific implementation manner, a heat-sealed part 212 is further formed on the sealing part 21 above the zipper 211. The heat-sealed part 212 is open and can be sealed after heat-sealing treatment. The zipper 11 and the heat-sealed part 212 are used to achieve double sealing. Since the latter is more reliable, this embodiment of the application is illustrated by the latter.

[0024] The implementation principle of a cell recovery device in an embodiment of this application is as follows: Keep the heat-sealed part 212 and the zipper 211 open, insert the cryopreservation tubes into the warm bath sleeves 22 from top to bottom one by one, press the zipper 211 to close it with fingers, and then perform heat-sealing. At this time, the recovery bag 2 has been sealed.

[0025] Next, clamp the resuscitation bag 2 in the groove 11 to fix it. Then, place the whole cell resuscitation device in a water bath that has been pre-heated in advance. When there is still a little ice in the cryopreservation tube, take out the resuscitation bag 2, unseal the heat-sealed part 212, open the zip fastener 211, take out the cryopreservation tube from the warm bath sleeve 22 and perform subsequent operations.

[0026] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereby. Therefore, all equivalent changes made according to the structure, shape, and principle of the present application should be covered within the protection scope of the present application.

Claims

1. A cell resuscitation device, characterized in that: The invention comprises a floating plate (1) and a resuscitation bag (2). The surface of the floating plate (1) is provided with a groove (11) for clamping the resuscitation bag (2). The resuscitation bag (2) comprises a sealing portion (21) and a plurality of warm bath sleeves (22) arranged at intervals at the lower end of the sealing portion (21). The warm bath sleeves (22) are provided for inserting cryopreservation tubes. The upper end of the sealing portion (21) is provided with a clamp chain (211).

2. A cell resuscitation device according to claim 1, characterized in that: The resuscitation bag (2) is provided with a thermoplastic sealing portion (212) above the clip chain (211).

3. A cell resuscitation device according to claim 1, characterized in that: The resuscitation bag (2) is a PE bag.

4. A cell resuscitation device according to claim 1, characterized in that: The warm bath sleeve (22) is a strip-shaped bag.

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