Cell exposure hypoxia environment experimental device

Abstract

The utility model discloses a cell exposure hypoxia environment experiment device, which relates to the technical field of cell hypoxia experiments and comprises a support base and a ventilation component, an experiment box is mounted at the front end of the upper surface of the support base, and the ventilation component is arranged on the rear surface of the experiment box. The ventilation assembly comprises a dustproof ventilation opening, a ventilator, a protection frame, a mounting part, a clamping hole, a clamping part, a sealing plate and a rubber sealing gasket, the dustproof ventilation opening is formed in the lower end of the rear surface of the experiment box, the ventilator is mounted on the rear side of the dustproof ventilation opening, and the protection frame is arranged on the outer side of the ventilator. According to the cell exposure hypoxia environment experiment device, rapid ventilation can be achieved through the ventilation assembly, the recovery time of internal oxygen concentration and carbon dioxide concentration is shortened, concentration balance is ensured, multiple groups of cell experiments can be conducted at the same time through the limiting assembly according to needs, and the experiment efficiency is improved. And the cell culture dish can be limited, so that the cell culture dish is prevented from shaking or falling off during taking and placing.

Description

Technical Field

[0001] The utility model relates to the technical field of cell hypoxia experiments, and specifically relates to an experimental device for exposing cells to a hypoxic environment. Background Technique

[0002] The hypoxia experiment is an experiment used to study the physiological and biochemical changes of organisms under hypoxic conditions. In a hypoxic environment, the oxygen supply of organisms is insufficient, which will lead to a series of adaptations and reactions. The hypoxia experiment can help scientists better understand the coping mechanisms and survival abilities of organisms to hypoxia, and it is of great significance for studying respiratory diseases, high-altitude adaptability, and hypoxia-related cardiovascular diseases, etc. Therefore, an experimental device for exposing cells to a hypoxic environment is needed to facilitate the hypoxia experiment. However, the current experimental devices for exposing cells to a hypoxic environment still have the following deficiencies:

[0003] For example, the patent document with the application number 202221559260.9 discloses an experimental device for cell hypoxia. This experimental device for cell hypoxia, by opening the first intake valve and the first outlet valve, connecting the first intake valve to a gas cylinder filled with the test mixture gas, continuously introducing the mixture gas, the oxygen analyzer can accurately measure the oxygen concentration of the gas inside the sealed cover. When the oxygen concentration reaches the standard, the first intake valve and the first outlet valve are closed, thus establishing an experimental device for the cell hypoxic environment. However, after the experiment is completed, the oxygen and carbon dioxide concentrations inside cannot be quickly adjusted, which is not convenient for subsequent experiments.

[0004] Therefore, in view of this, research and improvement are carried out on the existing structural deficiencies, and an experimental device for exposing cells to a hypoxic environment is proposed. Content of the Utility Model

[0005] The purpose of the utility model is to provide an experimental device for exposing cells to a hypoxic environment to solve the problems raised in the above background technique.

[0006] To achieve the above purpose, the utility model provides the following technical solution: An experimental device for exposing cells to a hypoxic environment, including a support base and a ventilation component. The front end of the upper surface of the support base is equipped with an experimental chamber, and the ventilation component is arranged on the rear surface of the experimental chamber. The ventilation component includes a dust-proof ventilation opening, a ventilator, a protective frame, a mounting member, a clamping hole, a clamping member, a sealing plate, and a rubber sealing gasket. The dust-proof ventilation opening is opened at the lower end of the rear surface of the experimental chamber, and a ventilator is installed behind the dust-proof ventilation opening. A protective frame is arranged outside the ventilator, and mounting members are installed at the rear ends of both sides of the protective frame. Clamping holes are opened on the surface of the mounting member, and a clamping member is clamped and connected inside the clamping hole. The sealing plate is installed on the inner surface of the clamping member.

[0007] Furthermore, a rubber gasket is connected to the front surface of the sealing plate, and the inner side of the rubber gasket is connected to the rear end of the inner surface of the protective frame.

[0008] Furthermore, the experimental chamber is of an integrated structure, and a limiting component for limiting is arranged inside the experimental chamber.

[0009] Furthermore, the limiting component includes a limiting support frame, a support plate, a limiting chute, and a limiting slide bar. The limiting support frame is arranged on both inner surfaces of the experimental chamber. The support plate is slidably connected to the inner side of the limiting support frame, and a limiting chute is formed on the upper surface of the support plate. The limiting slide bar is slidably connected to the inner side of the limiting chute.

[0010] Furthermore, the limiting component further includes a movable plate, a fixed plate, a limiting groove, and an elastic protective pad. The movable plate is installed on the upper surface of the limiting slide bar. Fixed plates are arranged at both ends of the upper surface of the support plate. Limiting grooves are formed on the surfaces of the fixed plate and the movable plate. The elastic protective pad is connected to the inner surface of the limiting groove.

[0011] Furthermore, a protective door is connected to the front side of the experimental chamber by a hinge, and an elastic sealing pad is arranged on the rear surface of the protective door.

[0012] Furthermore, a support cylinder is installed at the rear end of the upper surface of the support base, and an air storage tank is arranged inside the support cylinder. An air valve is installed at the top of the air storage tank, and an air delivery pipe is connected to the front end of the air valve.

[0013] Furthermore, a sealing cylinder is arranged at the upper end of the rear surface of the experimental chamber, and the front end of the air delivery pipe is snap-connected to the inside of the sealing cylinder.

[0014] The present utility model provides a cell exposure hypoxia environment experimental device, which has the following beneficial effects:

[0015] 1. By providing a ventilation component, the ventilation component includes a dust-proof ventilation opening, a ventilator, a protective frame, a mounting member, a clamping hole, a clamping member, a sealing plate, and a rubber gasket. During use, the sealing plate is removed, and the ventilator is started, so that the ventilator can quickly ventilate the inside of the experimental chamber through the dust-proof ventilation opening. Before the experiment, the clamping member is fixed on the surface of the mounting member through the snap connection between the clamping member and the clamping hole, and the sealing plate seals the protective frame through the rubber gasket to prevent gas leakage. Thus, the device can achieve rapid ventilation, thereby shortening the recovery time of the internal oxygen concentration and carbon dioxide concentration and ensuring the balance of the concentration.

[0016] 2. The utility model is provided with a limiting component, which includes a limiting support frame, a support plate, a limiting chute, and a limiting slide bar. The limiting component further includes a movable plate, a fixed plate, a limiting groove, and an elastic protective pad. During use, move the movable plate so that the movable plate moves inward along the limiting chute through the limiting slide bar. Place the cell culture dish inside the limiting groove, and then push the movable plate. Drive the movable plate to reset through the sliding connection between the limiting slide bar and the limiting chute to fix and limit the culture dish. The elastic protective pad can improve the tightness of the limit, and the support plate can be flexibly taken out or installed through the sliding connection on both sides of it with the inner side of the limiting support frame, so that the device can conduct experiments on multiple groups of cells simultaneously as needed, and it can limit the cell culture dish to prevent it from shaking or falling during taking and placing. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a three-dimensional structural schematic diagram of an experimental device for exposing cells to an anoxic environment according to the utility model;

[0018] Figure 2 is a three-dimensional structural schematic diagram of the ventilation component of an experimental device for exposing cells to an anoxic environment according to the utility model;

[0019] Figure 3 is a three-dimensional structural schematic diagram of the experimental chamber of an experimental device for exposing cells to an anoxic environment according to the utility model;

[0020] Figure 4 is a three-dimensional structural schematic diagram of the limiting component of an experimental device for exposing cells to an anoxic environment according to the utility model.

[0021] In the figure: 1, support base; 2, experimental chamber; 3, ventilation component; 301, dust-proof ventilation opening; 302, ventilator; 303, protective frame; 304, mounting part; 305, clamping hole; 306, clamping part; 307, sealing plate; 308, rubber sealing gasket; 4, limiting component; 401, limiting support frame; 402, support plate; 403, limiting chute; 404, limiting slide bar; 405, movable plate; 406, fixed plate; 407, limiting groove; 408, elastic protective pad; 5, protective door; 6, elastic sealing gasket; 7, support cylinder; 8, gas storage tank; 9, gas valve; 10, gas pipeline; 11, sealing cylinder. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] The following further describes in detail the embodiments of the present utility model in conjunction with the drawings and examples. The following examples are used to illustrate the present utility model, but cannot be used to limit the scope of the present utility model.

[0023] As Figures 1 to 4As shown in the figure, an experimental device for exposing cells to a hypoxic environment includes a support base 1 and a ventilation component 3. At the front end of the upper surface of the support base 1, an experimental chamber 2 is installed. The ventilation component 3 is arranged on the rear surface of the experimental chamber 2, and the ventilation component 3 includes a dust-proof ventilation opening 301, a ventilator 302, a protective frame 303, a mounting part 304, a clamping hole 305, a clamping part 306, a sealing plate 307, and a rubber sealing gasket 308. The dust-proof ventilation opening 301 is opened at the lower end of the rear surface of the experimental chamber 2, and a ventilator 302 is installed at the rear side of the dust-proof ventilation opening 301. A protective frame 303 is arranged outside the ventilator 302, and mounting parts 304 are installed at the rear ends of both sides of the protective frame 303. Clamping holes 305 are opened on the surface of the mounting parts 304, and a clamping part 306 is snap-connected inside the clamping holes 305. A sealing plate 307 is installed on the inner surface of the clamping part 306.

[0024] The specific operation is as follows. When in use, remove the sealing plate 307 and start the ventilator 302, so that the ventilator 302 can quickly ventilate the inside of the experimental chamber 2 through the dust-proof ventilation opening 301, shorten the recovery time of the internal oxygen concentration and carbon dioxide concentration, and ensure the balance of the concentration. Before the experiment, fix the clamping part 306 on the surface of the mounting part 304 through the snap connection between the clamping part 306 and the clamping hole 305, so that the sealing plate 307 seals the protective frame 303 through the rubber sealing gasket 308 to prevent gas leakage.

[0025] Please refer to Figures 3 to 4 , a rubber sealing gasket 308 is connected to the front surface of the sealing plate 307, and the inner side of the rubber sealing gasket 308 is connected to the inner surface of the rear end of the protective frame 303. The experimental chamber 2 is of an integrated structure, and a limiting component 4 for limiting is arranged inside the experimental chamber 2. The limiting component 4 includes a limiting support frame 401, a support plate 402, a limiting sliding groove 403, and a limiting sliding strip 404. The limiting support frame 401 is arranged on both side surfaces inside the experimental chamber 2. A support plate 402 is slidably connected inside the limiting support frame 401. A limiting sliding groove 403 is opened on the upper surface of the support plate 402, and a limiting sliding strip 404 is slidably connected inside the limiting sliding groove 403. The limiting component 4 further includes a movable plate 405, a fixing plate 406, a limiting groove 407, and an elastic protective pad 408. A movable plate 405 is installed on the upper surface of the limiting sliding strip 404. Fixing plates 406 are arranged at both ends of the upper surface of the support plate 402. Limiting grooves 407 are opened on the surfaces of the fixing plates 406 and the movable plate 405, and an elastic protective pad 408 is connected to the inner surface of the limiting grooves 407;

[0026] The specific operation is as follows. When in use, move the movable plate 405 so that the movable plate 405 moves inward along the limiting chute 403 through the limiting slide bar 404. Place the cell culture dish inside the limiting groove 407. Push the movable plate 405, and drive the movable plate 405 to reset through the sliding connection between the limiting slide bar 404 and the limiting chute 403 to fix and limit the culture dish. The elastic protective pad 408 can improve the tightness of the limit, and the support plate 402 can be flexibly taken out or installed through the sliding connection between its two sides and the inner side of the limiting support frame 401.

[0027] Please refer to Figures 1 to 3 , a protective door 5 is hinged to the front side of the experimental box 2, and an elastic sealing pad 6 is arranged on the rear surface of the protective door 5. A support cylinder 7 is installed at the rear end of the upper surface of the support base 1, and an air storage tank 8 is arranged inside the support cylinder 7. An air valve 9 is installed on the top of the air storage tank 8, and an air delivery pipe 10 is connected to the front end of the air valve 9. A sealing cylinder 11 is arranged at the upper end of the rear surface of the experimental box 2, and the front end of the air delivery pipe 10 is snap-fitted and connected to the inside of the sealing cylinder 11;

[0028] The specific operation is as follows. When in use, close the protective door 5, seal the experimental box 2 through the elastic sealing pad 6, connect the air delivery pipe 10 to the rear side of the experimental box 2 through the snap-fitting connection between the sealing cylinder 11 and the front end of the air delivery pipe 10. After opening the air valve 9, make the carbon dioxide in the air storage tank 8 be transported into the experimental box 2 through the air delivery pipe 10 to facilitate creating an anoxic experimental environment.

[0029] In summary, as Figures 1 to 4As shown, for the experimental device for exposing cells to an anoxic environment, during use, first, move the movable plate 405 so that the movable plate 405 moves inward along the limiting chute 403 through the limiting slide bar 404. Place the cell culture dish inside the limiting groove 407, and then push the movable plate 405. The sliding connection between the limiting slide bar 404 and the limiting chute 403 drives the movable plate 405 to reset, fixing and limiting the culture dish. The elastic protective pad 408 can improve the tightness of the limit. The support plate 402 can be flexibly removed or installed through the sliding connection between its two sides and the inner side of the limiting support frame 401. Subsequently, close the protective door 5, seal the experimental chamber 2 through the elastic sealing pad 6, connect the air delivery pipe 10 to the rear side of the experimental chamber 2 through the snap connection between the sealing cylinder 11 and the front end of the air delivery pipe 10. After opening the air valve 9, the carbon dioxide in the gas storage tank 8 is conveyed into the experimental chamber 2 through the air delivery pipe 10 to facilitate the creation of an anoxic experimental environment. After the experiment is completed, take out the cells for the experiment, remove the sealing plate 307, and start the ventilator 302 so that the ventilator 302 realizes rapid ventilation of the inside of the experimental chamber 2 through the dust-proof ventilation opening 301, shortening the recovery time of the internal oxygen concentration and carbon dioxide concentration and ensuring the balance of the concentration. Before the experiment, fix the clamping member 306 on the surface of the mounting member 304 through the snap connection between the clamping member 306 and the clamping hole 305, and seal the protective frame 303 with the sealing plate 307 through the rubber sealing pad 308 to prevent gas leakage during subsequent experiments.

[0030] The embodiments of the present invention are given for purposes of illustration and description, and are not exhaustive or limit the present invention to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are chosen and described in order to better illustrate the principles of the present invention and its practical applications, and to enable those of ordinary skill in the art to understand the present invention and design various embodiments with various modifications suitable for specific purposes.

Claims

1. A cell exposure hypoxic environment experimental device, comprising a support base (1) and a ventilation component (3), characterized in that: The front end of the upper surface of the support base (1) is equipped with an experimental box (2); the ventilation assembly (3) is arranged on the rear surface of the experimental box (2); and the ventilation assembly (3) comprises a dustproof vent (301), a ventilator (302), a protective frame (303), a mounting member (304), a snap-on hole (305), a snap-on member (306), a sealing plate (307) and a rubber sealing pad (308); the dustproof vent (301) is opened at the rear surface of the experimental box (2); A fan (302) is installed at the lower end of the surface and on the rear side of the dustproof ventilation port (301); a protective frame (303) is arranged on the outside of the fan (302); and mounting members (304) are installed on the rear ends of both sides of the protective frame (303); a snap-in hole (305) is opened on the surface of the mounting member (304); and a snap-in member (306) is snap-connected to the inner side of the snap-in hole (305); and a sealing plate (307) is installed on the inner surface of the snap-in member (306).

2. The cell exposure hypoxia environment experimental device according to claim 1, characterized in that: The front surface of the sealing plate (307) is connected to a rubber sealing pad (308), and the inner side of the rubber sealing pad (308) is connected to the rear end of the inner surface of the protection frame (303).

3. The cell exposure hypoxic environment experimental device according to claim 1, characterized in that: The experimental box (2) is an integrated structure, and a position limiting component (4) for limiting position is arranged inside the experimental box (2).

4. The cell exposure hypoxic environment experimental device according to claim 3, characterized in that: The limiting assembly (4) comprises a limiting support frame (401), a support plate (402), a limiting slide groove (403), and a limiting slide bar (404), and the limiting support frame (401) is arranged on the inner two side surfaces of the experimental box (2), the inner side of the limiting support frame (401) is slidably connected to the support plate (402), and the upper surface of the support plate (402) is provided with a limiting slide groove (403), and the inner side of the limiting slide groove (403) is slidably connected to the limiting slide bar (404).

5. The cell exposure hypoxic environment experimental device according to claim 4, characterized in that: The limiting assembly (4) further comprises a movable plate (405), a fixed plate (406), a limiting groove (407) and an elastic protective pad (408), and the movable plate (405) is installed on the upper surface of the limiting slide bar (404), the fixed plates (406) are arranged at both ends of the upper surface of the support plate (402), and the limiting groove (407) is provided on the surface of the fixed plate (406) and the movable plate (405), and the inner surface of the limiting groove (407) is connected to the elastic protective pad (408).

6. The cell exposure hypoxia environment experimental device according to claim 1, characterized in that: The front hinge of the experimental box (2) is connected to a protective door (5), and the rear surface of the protective door (5) is provided with an elastic sealing pad (6).

7. The cell exposure hypoxic environment experimental device according to claim 1, characterized in that: A support tube (7) is installed at the rear end of the upper surface of the support base (1), and a gas storage tank (8) is arranged inside the support tube (7). A gas valve (9) is installed on the top of the gas storage tank (8), and a gas pipe (10) is connected to the front end of the gas valve (9).

8. The cell exposure hypoxic environment experimental device according to claim 1, characterized in that: A sealing cylinder (11) is provided at the upper end of the rear surface of the experimental box (2), and the inner side of the sealing cylinder (11) is snap-connected with the front end of the air delivery pipe (10).

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