Cell culture dish capable of preventing cell necrosis

Abstract

The utility model relates to a cell culture dish, belongs to the technical field of cell culture, and particularly relates to a cell culture dish capable of preventing cell necrosis, which comprises a dish body, a fixed ring fixedly connected onto the dish body, a protective cover in threaded connection onto the fixed ring, a filter screen arranged in the dish body, and a liquid changing component arranged in the dish body, the liquid changing assembly comprises a liquid changing pipe, a sliding plug, a water through hole, a pressing pipe and a connecting ring; according to the culture dish, the auxiliary assembly fixes the filter screen in the dish body through cooperation of a clamping block, a limiting block, a through hole, a hydrophobic membrane, a protective cover, a protective pad and an anti-skid ring, the filter screen is prevented from shaking and damaging cells in the dish body in the moving process of the dish body, and the liquid changing assembly is used for changing liquid through cooperation of a liquid changing pipe, a sliding plug, a water through hole, a pressing pipe, a fixing block, a spring and a connecting ring. The device can complete liquid change without opening the protective cover, so that the contact area of cells and the environment is reduced, and cell necrosis can be effectively prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture dish for preventing cell necrosis. Background Technique

[0002] Cell culture refers to a method of simulating the in-vivo environment in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture is also called cell cloning technology, and its formal term in biology is cell culture technology. Whether for the entire bioengineering technology or one of its sub-technologies, namely biological cloning technology, cell culture is an essential process.

[0003] A Chinese patent with the publication number CN216786154U discloses a cell culture dish for single cells. The patent is provided with a filter screen, which enables the cell culture dish to filter cells well during liquid replacement, avoiding the cells in the cell culture dish being discharged together with the liquid during liquid replacement, thus greatly improving the practicability of the cell culture dish.

[0004] In the above technical solution, the device needs to open the dish cover for liquid replacement, and opening the dish cover easily causes the cells inside to be contaminated by the environment, resulting in cell necrosis.

[0005] Based on this, the utility model proposes a cell culture dish for preventing cell necrosis. Content of the Utility Model

[0006] In order to solve the above technical problems, the utility model proposes a cell culture dish for preventing cell necrosis, which can fix the filter screen through an auxiliary component to prevent the filter screen from shaking during movement and damaging the cells inside. Through the liquid replacement component, the device can complete liquid replacement without opening the protective cover, reducing the contact area between the cells and the environment, and effectively preventing cell necrosis.

[0007] The technical solution for achieving the purpose of the utility model is: a cell culture dish for preventing cell necrosis, including a dish body, a fixing ring is fixedly connected to the dish body, a protective cover is threadedly connected to the fixing ring, a filter screen is arranged inside the dish body, and a liquid replacement component is arranged inside the dish body. The liquid replacement component includes liquid replacement tubes, sliding plugs, water through holes, pressing tubes and connecting rings. Two liquid replacement tubes are fixedly connected to the dish body, two sliding plugs are respectively slidably connected to the two liquid replacement tubes, two water through holes are respectively opened on the two sliding plugs, two pressing tubes are respectively fixedly connected to the two sliding plugs, two pressing tubes are respectively slidably connected inside the two liquid replacement tubes, two connecting rings are respectively fixedly connected to the two pressing tubes, and an auxiliary component is arranged on the dish body.

[0008] Preferably, the liquid changing assembly further includes fixing blocks and springs. The four fixing blocks are respectively fixedly connected to two pressing tubes, and the two ends of the four springs are respectively fixedly connected to the fixing blocks and the dish body.

[0009] Preferably, the auxiliary assembly further includes a clamping block and a limiting block. The clamping block is fixedly connected to the dish body, and the four limiting blocks are fixedly connected inside the dish body.

[0010] Preferably, the auxiliary assembly further includes a through hole, which is opened on the protective cover.

[0011] Preferably, the auxiliary assembly further includes a hydrophobic membrane and a protective cover. The hydrophobic membrane is arranged in the through hole, and the protective cover is movably inserted into the protective cover.

[0012] Preferably, the auxiliary assembly further includes a protective pad and an anti-slip ring. The protective pad is fixedly connected to the dish body, and the two anti-slip rings are fixedly connected to the dish body.

[0013] Compared with the prior art, the remarkable advantages of this utility model are as follows:

[0014] First: In this utility model, the auxiliary assembly cooperates with the clamping block, limiting block, through hole, hydrophobic membrane, protective cover, protective pad and anti-slip ring to fix the filter screen in the dish body, preventing the filter screen from shaking and damaging the cells inside during the movement of the dish body.

[0015] Second: In this utility model, the liquid changing assembly cooperates with the liquid changing tube, sliding plug, water passing hole, pressing tube, fixing block, spring and connecting ring, enabling the device to complete liquid changing without opening the protective cover, reducing the contact area between the cells and the environment, and effectively preventing cell necrosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The following further explains the present utility model in conjunction with the drawings and embodiments:

[0017] Figure 1 is the three-dimensional structure schematic diagram of the present utility model;

[0018] Figure 2 is the internal structure sectional view of the present utility model;

[0019] Figure 3 is the internal structure schematic diagram of the present utility model;

[0020] Figure 4 is the three-dimensional internal structure schematic diagram of the auxiliary assembly in the present utility model;

[0021] Figure 5 is the three-dimensional internal structure schematic diagram of the liquid changing assembly in the present utility model.

[0022] Explanation of the reference numerals:

[0023] 1. Dish body; 2. Fixed ring; 3. Protective cover; 4. Filter screen; 5. Auxiliary component; 51. Clamping block; 52. Limiting block; 53. Through hole; 54. Hydrophobic membrane; 55. Protective cover; 56. Protective pad; 57. Anti-slip ring; 6. Liquid changing component; 61. Liquid changing tube; 62. Sliding plug; 63. Water through hole; 64. Pressing tube; 65. Fixed block; 66. Spring; 67. Connecting ring. Detailed implementation manner

[0024] The present utility model will be described in detail below. The technical solutions in the embodiments of the present utility model are clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

[0025] The present utility model provides a cell culture dish for preventing cell necrosis by improvement. The technical solution of the present utility model is as follows:

[0026] As Figures 1-5 shown, a cell culture dish for preventing cell necrosis includes a dish body 1. A fixed ring 2 is fixedly connected to the dish body 1. A protective cover 3 is threadedly connected to the fixed ring 2. The cross-section of the protective cover 3 is in an inverted "U" shape. A filter screen 4 is arranged in the dish body 1. A liquid changing component 6 is arranged in the dish body 1. The liquid changing component 6 includes a liquid changing tube 61, a sliding plug 62, a water through hole 63, a pressing tube 64 and a connecting ring 67. Two liquid changing tubes 61 are fixedly connected to the dish body 1. The liquid changing tubes 61 are located on both sides of the dish body 1. The liquid changing tube 61 at the bottom outside of the dish body 1 is used to discharge the culture solution in the dish body 1. The liquid changing tube 61 at the top outside of the dish body 1 is used to inject new culture solution. Two sliding plugs 62 are respectively slidably connected to the two liquid changing tubes 61. Two water through holes 63 are respectively opened on the two sliding plugs 62. The cross-section of the water through hole 63 is in a "T" shape. Two pressing tubes 64 are respectively fixedly connected to the two sliding plugs 62. Two pressing tubes 64 are respectively slidably connected in the two liquid changing tubes 61. Two connecting rings 67 are respectively fixedly connected to the two pressing tubes 64. The connecting ring 67 is used to connect to an external water pipe. The dish body 1 is provided with an auxiliary component 5.

[0027] Furthermore, as Figures 1-5 shown, the liquid changing component 6 further includes a fixed block 65 and a spring 66. Four fixed blocks 65 are respectively fixedly connected to the two pressing tubes 64. Two fixed blocks 65 are arranged on one pressing tube 64. The two fixed blocks 65 are symmetrical about the pressing tube 64. The two ends of the four springs 66 are respectively fixedly connected to the fixed block 65 and the dish body 1.

[0028] Furthermore, asFigures 2-4 As shown, the auxiliary component 5 further includes a clamping block 51 and a limiting block 52. The clamping block 51 is fixedly connected to the dish body 1. The cross-section of the clamping block 51 is in a "cross" shape. Four limiting blocks 52 are fixedly connected inside the dish body 1, and the limiting blocks 52 are distributed in an annular array with the center of the dish body 1 as the center of the circle.

[0029] Further, as Figure 1 and Figure 2 shown, the auxiliary component 5 further includes a through hole 53. The through hole 53 is opened on the protective cover 3. The through hole 53 is used for ventilation. The center of the through hole 53, the center of the dish body 1, and the center of the filter net 4 are located on the same vertical line.

[0030] Further, as Figure 1 and Figure 2 shown, the auxiliary component 5 further includes a hydrophobic membrane 54 and a protective cover 55. The hydrophobic membrane 54 is arranged in the through hole 53. The hydrophobic membrane 54 can well prevent the contamination of cells by microorganisms during ventilation. The protective cover 55 is movably inserted into the protective cover 3.

[0031] Further, as Figures 1-4 shown, the auxiliary component 5 further includes a protective pad 56 and an anti-slip ring 57. The protective pad 56 is fixedly connected to the dish body 1. The protective pad 56 is made of rubber, with a soft texture, and can better protect the bottom of the dish body 1. Two anti-slip rings 57 are fixedly connected to the dish body 1.

[0032] The specific working method is as follows: Put the culture medium into the dish body 1, put the filter net 4 into the dish body 1, so that the filter net 4 is located inside the four limiting blocks 52, and the clamping block 51 is embedded in the bottom of the filter net 4. Then put the cells into the filter net 4, rotate and tighten the protective cover 3, and place the cells in the dish body 1 for culture. When liquid change is needed, open the protective cover 55 to balance the air pressure inside and outside the dish body 1. Then press the pressing tube 64 at the bottom to make it squeeze the sliding plug 62 to move towards the inside of the dish body 1. When the fixed block 65 meets the outside of the liquid change tube 61, the end of the sliding plug 62 away from the pressing tube 64 is embedded in the dish body 1, and the water passing hole 63 on it is also embedded inside the dish body 1. The culture medium flows into the pressing tube 64 along the water passing hole 63, and then flows out of the dish body 1 along the pressing tube 64. When the culture medium is drained, release the pressing tube 64. Under the action of the spring 66, the sliding plug 62 resets and is embedded in the liquid change tube 61, and the water passing hole 63 on it is also embedded in the liquid change tube 61. At this time, connect the external water pipe to the connecting ring 67 at the top, press the pressing tube 64 at the top to make the water passing hole 63 embedded in the dish body 1, then pour the culture medium into the pressing tube 64 along the water pipe, and then flow from the pressing tube 64 into the sliding plug 62, and flow into the dish body 1 along the water passing hole 63 of the sliding plug 62, thus completing the liquid change.

[0033] The technical means disclosed by the solution of the present utility model are not limited to those disclosed by the above technical means, but also include technical solutions composed of equivalent replacements of the above technical features. Matters not covered by the present utility model are common general knowledge in the art.

Claims

1. A cell culture dish for preventing cell necrosis, comprising a dish body (1), a fixing ring (2) fixedly connected to the dish body (1), a protective cover (3) threadedly connected to the fixing ring (2), a filter screen (4) arranged inside the dish body (1), characterized in that: A fluid exchange component (6) is provided in the dish body (1), and the fluid exchange component (6) comprises a fluid exchange tube (61), a sliding plug (62), a water through hole (63), a pressing tube (64) and a connecting ring (67). The two fluid exchange tubes (61) are fixedly connected to the dish body (1), the two sliding plugs (62) are respectively slidably connected to the two fluid exchange tubes (61), the two water through holes (63) are respectively opened on the two sliding plugs (62), the two pressing tubes (64) are respectively fixedly connected to the two sliding plugs (62), the two pressing tubes (64) are respectively slidably connected to the two fluid exchange tubes (61), and the two connecting rings (67) are respectively fixedly connected to the two pressing tubes (64). The dish body (1) is provided with an auxiliary component (5).

2. A cell culture dish for preventing cell necrosis according to claim 1, characterized in that: The liquid replacement assembly (6) further comprises a fixed block (65) and a spring (66), wherein the four fixed blocks (65) are respectively fixedly connected to the two pressing tubes (64), and the two ends of the four springs (66) are respectively fixedly connected to the fixed block (65) and the dish body (1).

3. A cell culture dish for preventing cell necrosis according to claim 1, characterized in that: The auxiliary component (5) further comprises a clamping block (51) and a limiting block (52); the clamping block (51) is fixedly connected to the dish body (1); and four limiting blocks (52) are fixedly connected inside the dish body (1).

4. A cell culture dish for preventing cell necrosis according to claim 1, characterized in that: The auxiliary component (5) further comprises a through hole (53), wherein the through hole (53) is formed on the protective cover (3).

5. A cell culture dish for preventing cell necrosis according to claim 4, characterized in that: The auxiliary component (5) further comprises a hydrophobic membrane (54) and a protective cover (55); the hydrophobic membrane (54) is arranged in the through hole (53); and the protective cover (55) is movably plugged onto the protective cover (3).

6. A cell culture dish for preventing cell necrosis according to claim 1, characterized in that: The auxiliary component (5) further comprises a protective pad (56) and an anti-slip ring (57), wherein the protective pad (56) is fixedly connected to the dish body (1), and the two anti-slip rings (57) are fixedly connected to the dish body (1).

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