Sampling device for protein extraction

Abstract

The utility model relates to a sampling device for protein extraction, which comprises a working table, a plurality of support rods are arranged at the bottom of the working table, and a material containing module, a suction module and a sampling module are sequentially arranged on the upper surface of the working table from left to right; the material containing module comprises a container for containing a protein solution and a positioning block for placing the container; the suction module can suck a protein solution from the containing module and transfer the protein solution to the sampling module, and comprises a hollow supporting column, a first driving assembly, a mounting plate, a suction assembly and a second driving assembly; the sampling module comprises a third driving assembly, a rotating disc, a positioning cylinder and a sampling bottle; the device further comprises a controller connected with the first driving assembly, the second driving assembly, the third driving assembly and the suction assembly. According to the utility model, the automatic and quantitative absorption and transfer of the protein mixed liquid are realized, the operation efficiency and accuracy are greatly improved, the manual operation is reduced, and the pollution risk is reduced.

Description

Technical Field

[0001] The utility model relates to a sampling device, in particular to a sampling device for protein extraction, belonging to the technical field of sampling equipment. Background Art

[0002] In biochemical research and industrial production, the extraction of proteins is a key step. During the protein extraction process, it is often necessary to sample and analyze the broken protein mixture. Traditional sampling methods mostly use pipettes or similar tools to manually suck and transfer samples, which are not only cumbersome and time-consuming to operate, but also difficult to ensure the accuracy of the suction volume. In addition, manual operation is prone to introduce contamination and errors, affecting the accuracy of subsequent experimental results.

[0003] Therefore, a sampling device for protein extraction that can automatically and accurately suck and transfer protein mixtures is needed. Summary of the Utility Model

[0004] The utility model mainly aims at the above problems existing in the prior art, and provides a sampling device for protein extraction to solve the problems of cumbersome manual operation, inaccurate suction volume and easy contamination in the prior art.

[0005] The purpose of the utility model is mainly achieved through the following scheme:

[0006] A sampling device for protein extraction, including a workbench, multiple support rods are arranged at the bottom of the workbench, and a material holding module, a suction module and a sampling module are sequentially arranged on the upper surface of the workbench from left to right; the material holding module includes a container for holding a protein solution and a positioning block for placing the container; the suction module can suck the protein solution from the material holding module and transfer it to the sampling module. The suction module includes a hollow support column, a first driving component, a mounting plate, a suction component and a second driving component. The first driving component is installed on the workbench and can drive the support column to rotate. The second driving component is installed on the support column and can drive the mounting plate to move up and down. The suction component is installed on the mounting plate and is located above the container; the sampling module includes a third driving component, a rotating disk, a positioning cylinder and a sampling bottle. The third driving component is installed on the workbench and can drive the rotating disk to rotate. The positioning cylinder is installed on the rotating disk. A plurality of sampling bottles are provided and are all placed in the positioning cylinder; the sampling device further includes a controller connected to the first driving component, the second driving component, the third driving component and the suction component.

[0007] Preferably, the positioning block is installed on the upper surface of the workbench, and a first positioning groove matching the container is opened in the middle of the upper surface of the positioning block. Clamping components for clamping the container are symmetrically arranged on the upper surface of the workbench on the front and rear sides of the positioning block.

[0008] Preferably, the clamping assembly includes a fixing plate, a first telescopic assembly, and a clamping plate. The fixing plate is vertically installed on the upper surface of the workbench. The fixed end of the first telescopic assembly is installed on one side of the fixing plate facing the positioning block. The telescopic end of the first telescopic assembly is connected to one side of the clamping plate. The first telescopic assembly is connected to the controller.

[0009] Preferably, the clamping plate is an arc-shaped plate and is adapted to the outer wall of the container. A buffer pad is provided on the inner side of the clamping plate.

[0010] Preferably, the first driving assembly is installed on the lower surface of the workbench, and the output end of the first driving assembly passes through the workbench and is fixedly connected to the lower surface of the support column.

[0011] Preferably, the second driving assembly includes a second driving motor, a threaded rod, a threaded block, and a sliding block. The second driving motor is fixedly installed on the upper surface of the support column, and the output end of the second driving motor passes through the upper end of the support column and is fixedly connected to the threaded rod vertically arranged inside the support column. The bottom of the threaded rod is rotatably connected to the inner bottom of the support column. The threaded block is threadedly connected to the threaded rod.

[0012] Preferably, a chute is vertically opened on one side of the side wall of the support column facing the container. One side of the sliding block is fixedly connected to the threaded block, and the other side of the sliding block passes through the chute and is fixedly connected to the mounting plate.

[0013] Preferably, the suction assembly includes a second telescopic assembly, a support frame, a suction cylinder, a suction head, and a piston. The support frame is installed on the upper surface of the mounting plate. The fixed end of the second telescopic assembly is fixedly installed on the support frame. The two ends of the suction cylinder are open and the interior is hollow, and the upper end of the suction cylinder is fixedly installed on the lower surface of the mounting plate. The lower end of the suction cylinder is provided with a suction head. The piston is slidably connected inside the suction cylinder, and the telescopic end of the second telescopic assembly extends into the suction cylinder and is connected to the upper end of the piston.

[0014] Preferably, the third driving assembly is installed on the lower surface of the workbench, and the output end of the third driving assembly passes through the workbench and is fixedly connected to the lower surface of the rotating disk. The positioning cylinder is installed on the upper surface of the rotating disk, and the upper end of the positioning cylinder is open.

[0015] Preferably, an installation block is provided inside the positioning cylinder. A plurality of second positioning grooves are evenly arranged in a circular shape along the axis on the upper surface of the installation block. The sampling bottle is installed in the second positioning groove.

[0016] Therefore, compared with the prior art, the present utility model has the following advantages:

[0017] (1) The utility model realizes the automatic and quantitative suction and transfer of protein solution through the set material-containing module, suction module and sampling module, greatly improving the operation efficiency and accuracy.

[0018] (2) Through the set suction component, the utility model can ensure that the sample amount sucked each time is consistent, reduce the manual operation error, effectively avoid the risk of contamination, and ensure the purity of the sample.

[0019] (3) The utility model has a simple structure and convenient operation, and is applicable to a variety of biochemical experiment scenarios. Description of the Drawings

[0020] Figure 1 is a schematic structural diagram of the utility model;

[0021] Figure 2 is a schematic structural diagram of another angle of the utility model;

[0022] Figure 3 is the front view of the utility model;

[0023] Figure 4 is the side view of the utility model;

[0024] Figure 5 is the schematic internal structure diagram of the support column in the utility model;

[0025] Figure 6 is the schematic internal structure diagram of the suction cylinder in the utility model;

[0026] Figure 7 is the structural diagram of the positioning cylinder in the utility model.

[0027] Illustration: 1 - Workbench, 2 - Support rod, 3 - Container, 4 - Positioning block, 5 - Support column, 6 - First drive assembly, 7 - Mounting plate, 8 - Third drive assembly, 9 - Rotating disk, 10 - Positioning cylinder, 11 - Sampling bottle, 12 - Controller, 13 - First positioning groove, 14 - Fixing plate, 15 - First telescopic assembly, 16 - Clamping plate, 17 - Buffer pad, 18 - Second drive motor, 19 - Threaded rod, 20 - Threaded block, 21 - Sliding block, 22 - Chute, 23 - Second telescopic assembly, 24 - Support frame, 25 - Suction cylinder, 26 - Suction head, 27 - Piston, 28 - Mounting block, 29 - Second positioning groove. Detailed Embodiments

[0028] The following are specific embodiments, and in combination with the drawings, the technical solutions of the utility model will be further specifically described. It should be understood that the implementation of the utility model is not limited to the following embodiments, and any form of modification and / or change made to the utility model will fall within the protection scope of the utility model.

[0029] In the present utility model, unless otherwise specified, all parts and percentages are in weight units, and the equipment and raw materials used can be purchased from the market or are commonly used in the art. The methods in the following embodiments are conventional methods in the art unless otherwise specified. The components or equipment in the following embodiments are all general standard parts or components known to those skilled in the art, and their structures and principles can be known by those skilled in the art through technical manuals or by conventional experimental methods.

[0030] Embodiment 1:

[0031] As Figure 1 、 2 shown, the present utility model provides a technical solution for a sampling device for protein extraction, including a workbench 1. A plurality of support rods 2 are fixedly installed at the bottom of the workbench 1 through bolts. The support rods 2 can adopt common adjustable feet on the market to maintain the stability and reliability of the workbench 1. A material-containing module, a suction module, and a sampling module are sequentially arranged on the upper surface of the workbench 1 from left to right.

[0032] The above-mentioned material-containing module includes a container 3 for containing a protein solution and a positioning block 4 for placing the container 3. The setting of the positioning block 4 facilitates the placement and positioning of the container 3.

[0033] The above-mentioned suction module can suck the protein solution from the material-containing module and transfer it to the sampling module. The suction module is composed of a hollow support column 5, a first driving component 6, a mounting plate 7, a suction component, and a second driving component. The support column 5 is vertically installed on the upper surface of the workbench 1. The first driving component 6 is installed on the workbench 1 and can drive the support column 5 to rotate. The second driving component is installed on the support column 5 and can drive the mounting plate 7 to move up and down. The mounting plate 7 faces the positioning block 4. The suction component is installed on the mounting plate 7 and is located above the container 3. The suction component can suck the protein solution in the container 3 and rotate the suction component to the sampling module through the first driving component 6 and the support column 5, thereby realizing the suction and transfer of the protein solution.

[0034] The above-mentioned sampling module is composed of a third driving component 8, a rotating disk 9, a positioning cylinder 10, and a sampling bottle 11. The third driving component 8 is installed on the workbench 1 and can drive the rotating disk 9 to rotate. The positioning cylinder 10 is installed on the rotating disk 9. A plurality of sampling bottles 11 are provided and are all placed in the positioning cylinder 10. The sampling bottles 11 are evenly arranged in a circumferential manner along the axis of the positioning cylinder 10.

[0035] The sampling device further includes a controller 12 connected to the first driving component 6, the second driving component, the third driving component 8, and the suction component.

[0036] Embodiment 2:

[0037] As Figure 1 、 4 shown, the present utility model provides another technical solution, a sampling device for protein extraction. Different from Embodiment 1, the positioning block 4 is fixedly installed on the upper surface of the workbench 1 by bolts, and a first positioning groove 13 matching the container 3 is provided in the middle of the upper surface of the positioning block 4. The container 3 can be taken away from the first positioning groove 13 of the positioning block 4. Clamping components for clamping the container 3 are symmetrically arranged on the front and rear sides of the positioning block 4 on the upper surface of the workbench 1.

[0038] Specifically, the clamping component is composed of a fixing plate 14, a first telescopic component 15 and a clamping plate 16. The fixing plate 14 is vertically installed on the upper surface of the workbench 1 by bolts. The fixed end of the first telescopic component 15 is longitudinally installed on the side of the fixing plate 14 facing the positioning block 4 by bolts. The telescopic end of the first telescopic component 15 is connected to one side of the clamping plate 16 by bolts. The first telescopic component 15 is connected to the controller 12. The first telescopic component 15 can adopt a push rod motor, a hydraulic cylinder or a cylinder, and preferably a push rod motor.

[0039] Specifically, the clamping plate 16 adopts an arc-shaped plate and is adapted to the outer wall of the container 3. A buffer pad 17 made of silica gel is adhesively bonded to the inner side of the clamping plate 16. On the one hand, it avoids scratches on the container, and on the other hand, it can achieve a good clamping effect on the container 3.

[0040] Embodiment 3:

[0041] As Figure 1 、 4 shown, the present utility model provides another technical solution, a sampling device for protein extraction. Different from Embodiment 1, the first driving component 6 adopts a first driving motor, which is vertically installed on the lower surface of the workbench 1 by bolts, and the output end of the first driving component passes through the workbench 1 and is fixedly connected to the lower surface of the support column 5 by bolts; by starting the first driving component, the support column 5 and the mounting plate 7 can be driven to rotate horizontally.

[0042] Specifically, as Figure 5 shown, the second driving component is composed of a second driving motor 18, a threaded rod 19, a threaded block 20 and a sliding block 21. The second driving motor 18 is vertically and fixedly installed on the upper surface of the support column 5 by bolts, and the output end of the second driving motor 18 passes through the upper end of the support column 5 and is fixedly connected to the upper end of the threaded rod 19 vertically arranged in the support column 5. The bottom of the threaded rod 19 is rotatably connected to the inner bottom of the support column 5 through a bearing. The middle of the threaded block 20 is threadedly connected to the threaded rod 19 through a threaded hole.

[0043] Specifically, a chute 22 is vertically formed in the side wall of the support column 5 facing the container 3. One side of the sliding block 21 is fixedly connected to the threaded block 20 by a bolt. The other side of the sliding block 21 passes through the chute 22 and is fixedly connected to the mounting plate 7 by a bolt. The front and rear side walls of the sliding block 21 are in contact with the front and rear inner walls of the chute 22. By starting the second driving motor 18, under the action of the threaded rod 19, the threaded block 20 and the sliding block 21, the mounting plate 7 can be driven to move up and down.

[0044] Specifically, as Figure 6 shown, the suction assembly is composed of a second telescopic assembly 23, a support frame 24, a suction cylinder 25, a suction head 26 and a piston 27. The support frame 24 is fixedly installed on the upper surface of the mounting plate 7 by bolts. The second telescopic assembly 23 is a push rod motor, and its fixed end is fixedly installed on the support frame 24 by bolts. Both ends of the suction cylinder 25 are open and the interior is hollow. The upper end of the suction cylinder 25 is fixedly installed on the lower surface of the mounting plate 7 by bolts. The lower end of the suction cylinder 25 is fixedly installed with a suction head 26. The piston 27 is slidably connected in the suction cylinder 25. The telescopic end of the second telescopic assembly 23 passes through the mounting plate 7 and extends into the suction cylinder 25 and is connected to the upper end of the piston 27. The structures of the suction cylinder 25, the suction head 26 and the piston 27 are similar to those of common syringes on the market at present. By starting the second telescopic assembly 23, the piston 27 can be driven to move up and down, thereby realizing the suction and release of the protein solution.

[0045] Embodiment 4:

[0046] As Figure 1 、 3 、7 shows, the present utility model provides another technical solution, a sampling device for protein extraction, which is different from that of Embodiment 1 in that the third driving assembly 8 is a third driving motor, fixedly installed on the lower surface of the workbench 1 by bolts, and the output end of the third driving assembly 8 passes through the workbench 1 and is fixedly connected to the lower surface of the rotating disk 9 by bolts. The positioning cylinder 10 is fixedly installed on the upper surface of the rotating disk 9 by bolts, and the upper end of the positioning cylinder 10 is open.

[0047] Specifically, a cylindrical mounting block 28 is fixedly installed inside the positioning cylinder 10. A circle of second positioning grooves 29 adapted to the sampling bottle 11 are evenly arranged in a circumferential manner along the axis of the upper surface of the mounting block 28. The sampling bottle 11 is placed in the second positioning grooves 29. In this embodiment, there are six second positioning grooves 29, and the distribution of the second positioning grooves 29 is as follows: two are symmetrically arranged on the left and right, and two are arranged on the front side and the rear side respectively and are symmetrically distributed.

[0048] The sampling device for protein extraction provided by the present utility model works as follows: 1. Place the crushed protein mixture into the container 3. Place the container 3 on the positioning block 4 through the first positioning groove 13, and then control the two first telescopic components 15 to work through the controller 12, driving the clamping plate 16 to clamp the container 3 towards the middle; 2. Start the second driving motor 18 through the controller 12, control the mounting plate 7 and the suction component to move downward to an appropriate position, and insert the suction head 26 into the protein mixture in the container 3; 3. Preset the movement / suction parameters of the second telescopic component 23 in advance. Start the second telescopic component 23 through the controller 12 and drive the piston 27 to move upward to the set position to accurately control the suction volume, and suck a predetermined amount of protein mixture into the suction cylinder 25; 4. Start the second driving motor 18 through the controller 12, control the mounting plate 7 and the suction component to move upward to an appropriate position, and then start the first driving component 6 through the controller 12, driving the support column 5 to rotate 180 degrees clockwise or counterclockwise; 5. Start the second driving motor 18 through the controller 12, control the mounting plate 7 and the suction component to move downward to an appropriate position, insert the suction head 26 into the rightmost sampling bottle 11, start the second telescopic component 23 through the controller 12 and drive the piston 27 to move downward to the bottom position inside the suction cylinder 25, and release the protein mixture into the sampling bottle 11, thus completing the sampling of the sample; 6. After the sampling is completed, start the second driving motor 12 through the controller 12, control the mounting plate 7 and the suction component to move upward to a suitable position, start the third driving component 8 through the controller 12, drive the rotating disk 9 and the positioning cylinder 10 to rotate 60 degrees clockwise or counterclockwise, start the first driving component 6 through the controller 12, and drive the support column 5 to rotate 180 degrees clockwise or counterclockwise, and then repeat steps 2-6 until all the sampling bottles 11 are completed with sampling.

[0049] The controller 12 in the present utility model adopts a PLC controller or an industrial control computer. The controller 12 is electrically connected to the first driving component, the second driving motor, the third driving component, the first telescopic component and the second telescopic component. The work of the first driving component, the second driving motor, the third driving component, the first telescopic component and the second telescopic component is controlled through the controller. The specific model specifications of the first driving component, the second driving motor, the third driving component, the first telescopic component and the second telescopic component need to be selected and determined according to the actual specifications of the device, etc. The specific selection calculation method adopts the existing technology in the field, so it will not be elaborated in detail. The specific structure and circuit of the controller are not within the protection scope of this application, and those skilled in the art can obtain them through the common general knowledge, conventional means or simple derivation in the field, so it will not be elaborated here.

[0050] The sampling device for protein extraction provided by the present utility model realizes the automatic and quantitative aspiration and transfer of protein solutions through the set material-containing module, aspiration module and sampling module, greatly improving the operation efficiency and accuracy; through the set aspiration assembly, the amount of sample aspirated each time can be ensured to be consistent, reducing the manual operation error and effectively avoiding the risk of contamination, and ensuring the purity of the sample; the structure of the present utility model is simple and the operation is convenient, and it is applicable to a variety of biochemical experimental scenarios.

[0051] It should be understood that this embodiment is only used to illustrate the present utility model and not to limit the scope of the present utility model. In addition, it should be understood that after reading the content taught by the present utility model, those skilled in the art can make various changes or modifications to the present utility model, and these equivalent forms also fall within the scope defined by the appended claims of this application.

Claims

1. A sampling device for protein extraction, comprising a workbench (1), wherein a plurality of support rods (2) are provided at the bottom of the workbench (1), characterized in that: The upper surface of the workbench (1) is provided with a material holding module, a suction module and a sampling module in sequence from left to right; The material holding module comprises a container (3) for holding a protein solution and a positioning block (4) for placing the container (3); The suction module can absorb protein solution from the material storage module and transfer it to the sampling module. The suction module comprises a support column (5) with a hollow interior, a first driving component (6), a mounting plate (7), a suction component and a second driving component. The first driving component (6) is mounted on the workbench (1) and can drive the support column (5) to rotate. The second driving component is mounted on the support column (5) and can drive the mounting plate (7) to move up and down. The suction component is mounted on the mounting plate (7) and is located above the container (3). The sampling module comprises a third driving component (8), a rotating disk (9), a positioning cylinder (10) and a sampling bottle (11); the third driving component (8) is mounted on the workbench (1) and is capable of driving the rotating disk (9) to rotate; the positioning cylinder (10) is mounted on the rotating disk (9); a plurality of sampling bottles (11) are provided and are all placed in the positioning cylinder (10); The sampling device also includes a controller (12) connected to the first driving component (6), the second driving component, the third driving component (8) and the suction component.

2. The sampling device for protein extraction according to claim 1, characterized in that: The positioning block (4) is mounted on the upper surface of the workbench (1), and a first positioning groove (13) matching the container (3) is provided in the middle of the upper surface of the positioning block (4). Clamping components for clamping the container (3) are symmetrically provided on the upper surface of the workbench (1) at the front and rear sides of the positioning block (4).

3. The sampling device for protein extraction according to claim 2, characterized in that: The clamping assembly comprises a fixed plate (14), a first telescopic assembly (15) and a clamping plate (16); the fixed plate (14) is vertically mounted on the upper surface of the workbench (1); the fixed end of the first telescopic assembly (15) is mounted on a side of the fixed plate (14) facing the positioning block (4); the telescopic end of the first telescopic assembly (15) is connected to one side of the clamping plate (16); and the first telescopic assembly (15) is connected to a controller (12).

4. The sampling device for protein extraction according to claim 3, characterized in that: The clamping plate (16) is an arc-shaped plate and is matched with the outer wall of the container (3). A buffer pad (17) is provided on the inner side of the clamping plate (16).

5. The sampling device for protein extraction according to claim 1, characterized in that: The first drive component (6) is mounted on the lower surface of the workbench (1), and the output end of the first drive component passes through the workbench (1) and is fixedly connected to the lower surface of the support column (5).

6. The sampling device for protein extraction according to claim 5, characterized in that: The second driving assembly comprises a second driving motor (18), a threaded rod (19), a threaded block (20) and a sliding block (21); the second driving motor (18) is fixedly mounted on the upper surface of the support column (5); the output end of the second driving motor (18) passes through the upper end of the support column (5) and is fixedly connected to the threaded rod (19) vertically arranged inside the support column (5); the bottom of the threaded rod (19) is rotatably connected to the bottom of the support column (5); and the threaded block (20) is threadedly connected to the threaded rod (19).

7. The sampling device for protein extraction according to claim 6, characterized in that: A sliding groove (22) is vertically provided on the side wall of the support column (5) facing the container (3); one side of the sliding block (21) is fixedly connected to the threaded block (20); and the other side of the sliding block (21) passes through the sliding groove (22) and is fixedly connected to the mounting plate (7).

8. The sampling device for protein extraction according to claim 7, characterized in that: The suction assembly comprises a second telescopic assembly (23), a support frame (24), a suction cylinder (25), a suction head (26) and a piston (27); the support frame (24) is mounted on the upper surface of the mounting plate (7); the fixed end of the second telescopic assembly (23) is fixedly mounted on the support frame (24); both ends of the suction cylinder (25) are open and the interior is hollow; the upper end of the suction cylinder (25) is fixedly mounted on the lower surface of the mounting plate (7); the lower end of the suction cylinder (25) is equipped with a suction head (26); the piston (27) is slidably connected to the suction cylinder (25); and the telescopic end of the second telescopic assembly (23) extends into the suction cylinder (25) and is connected to the upper end of the piston (27).

9. The sampling device for protein extraction according to claim 1, characterized in that: The third drive assembly (8) is mounted on the lower surface of the workbench (1), and the output end of the third drive assembly (8) passes through the workbench (1) and is fixedly connected to the lower surface of the rotating disk (9), and the positioning cylinder (10) is mounted on the upper surface of the rotating disk (9), and the upper end of the positioning cylinder (10) is open.

10. The sampling device for protein extraction according to claim 9, characterized in that: A mounting block (28) is provided inside the positioning cylinder (10), and a plurality of second positioning grooves (29) are evenly arranged on the upper surface of the mounting block (28) along the axis thereof, and the sampling bottle (11) is installed in the second positioning groove (29).

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