Production device for in-vitro culture of scylla paramamosain embryos

Abstract

The utility model belongs to the technical field of aquaculture, and particularly relates to a production device for in-vitro cultivation of scylla paramamosain embryos, which comprises a conical barrel, a rubber ring, a screen, a handle, a cylindrical base, a drain pipe and an oxygenating device, a drain hole is arranged at the bottom of the conical barrel provided with the handle, and the bottom of the drain hole is connected with a PVC (polyvinyl chloride) water pipe. A row of aquaculture water switches are arranged at the tail end of the PVC water pipe; two rubber ring screens with different diameters, which are made of rubber rings and screens, are embedded at different positions of the conical barrel, the small rubber ring screen at the lower part can prevent the in-vitro embryo of the scylla paramamosain from sinking at the bottom of the conical barrel, and the large rubber ring screen at the upper part can prevent the embryo from being taken out of the water surface to be adhered to the barrel wall of the conical barrel during inflation; meanwhile, in-vitro embryos can be prevented from being discharged by siphoning when water is changed; oxygen needed by the embryos in the cultivation process can be guaranteed through air pipe holes located in the small rubber ring screen and the bottom drainage hole, and the embryos are suspended in the cultivation water by adjusting an air valve switch.

Description

Technical Field

[0001] The utility model belongs to the technical field of aquaculture, and particularly relates to a production device for in vitro cultivation of Scylla pseudoburrowing embryos. Background Art

[0002] The mud crab is a large and fast-growing species in the family Portunidae. It has delicious meat and high nutritional value. It is an important aquatic species in aquaculture in my country's coastal areas. Compared with river crabs and swimming crabs, the artificial propagation technology of mud crab seedlings is relatively backward, and its seedling production still mainly depends on wild seedlings caught at sea. The yield and time of listing of wild seedlings are significantly affected by temperature and rainfall, which seriously restricts the large-scale development of the mud crab farming industry. Therefore, solving the problems existing in the artificial breeding and embryo culture process of mud crabs and achieving large-scale stable production and mass production of artificial mud crab seedlings are powerful means to promote the healthy development of the mud crab farming industry.

[0003] The breeding process of mud crab includes many links such as fattening of seed crabs, management of brooding crabs, and seed production. Since the seed crabs have problems such as decreased activity, poor feeding, weak physique, and susceptibility to infection with pathogenic microorganisms after brooding eggs, the breeding process often encounters the phenomenon of large-scale death of brooding crabs before the embryos hatch into zoeae, which eventually leads to the failure of mud crab breeding. Studies have found that after the death of brooding crabs, the embryos in their umbilicus are still alive for 4-5 hours. Therefore, if the embryos can be separated from the umbilicus of brooding crabs that died in a short period of time or have poor vitality, and hatched in vitro, it will be helpful for artificial breeding of mud crabs, thereby reducing the dependence on wild sea-caught mud crab seedlings. The in vitro culture device for fish embryos is usually designed as a planar structure, which is difficult to adapt to the characteristics of sedimentation and suspension of crustacean embryos in water. Compared with the in vitro culture technology of embryos of other aquatic animals such as fish, the in vitro culture technology of embryos of crustaceans is relatively backward. Although researchers have conducted relevant research on the in vitro culture technology of blue crab embryos before, there are still many problems in achieving mass production of in vitro culture of blue crab embryos and promoting its industrial application.

[0004] In view of the above analysis, the existing technology has the following technical problems that need to be solved urgently: compared with the in vitro embryo cultivation technology of other aquatic animals such as fish, the in vitro embryo cultivation technology of crustaceans is relatively backward. Although researchers have conducted relevant research on the in vitro embryo cultivation technology of blue crabs, their results still have many problems in achieving mass production of in vitro embryo cultivation of blue crabs and promoting its industrial application. Utility Model Content

[0005] In view of the problems existing in the prior art, the utility model provides a production device for in vitro cultivation of blue crab embryos. On the basis of the prior art, the blue crab embryo in vitro cultivation device is developed according to the size of blue crab embryos, the sedimentation characteristics of embryos in aquaculture seawater, and the demand for external environmental factors such as water quality and dissolved oxygen. Compared with the embryos relying on natural conditions and the natural hatching of brooding crabs, the device can not only greatly improve the in vitro hatching rate of blue crab embryos, but also increase the total number of embryos in vitro culture, thereby promoting the development of the blue crab artificial seedling industrialization.

[0006] The utility model is realized as follows: a production device for in vitro culture of mud crab embryos, which is composed of a conical barrel, a rubber ring, a screen, a handle, a cylindrical base, a drain pipe and an oxygenating device. The bottom of the conical barrel equipped with a handle is provided with a drain hole, the bottom of the drain hole is connected to a PVC water pipe, and the end of the PVC water pipe is provided with a row of aquaculture water switches; two rubber ring screens with different diameters made of rubber rings and screens are inlaid at different positions of the conical barrel, the small rubber ring screen located at the bottom can prevent the in vitro embryos of mud crabs from sinking to the bottom of the conical barrel, the large rubber ring screen located at the top can prevent the embryos from being brought out of the water and adhering to the wall of the conical barrel during inflation, and can also prevent the in vitro embryos from being siphoned out during water change; the tracheal holes located at the small rubber ring screen and the bottom drain hole can ensure the oxygen required by the embryos during the culture process, and the embryos are suspended in the aquaculture water by adjusting the air valve switch.

[0007] Furthermore, the opening diameter of the conical barrel is 110 cm and the barrel depth is 70 cm.

[0008] Further, the cylindrical base has a diameter of 70cm and a height of 60cm.

[0009] Furthermore, the diameter of the large rubber ring screen is 100 cm and the screen mesh number is 60 meshes.

[0010] Furthermore, the diameter of the small rubber ring screen is 10 cm and the screen mesh number is 60 meshes.

[0011] Furthermore, the outer diameter of the plastic tube is 10 mm; the inner diameter of the air tube is 9 mm, and it is equipped with an air regulating valve of a suitable size and a cake-shaped air stone with a diameter of 6 cm.

[0012] Furthermore, the PVC drain pipe has a diameter of 32mm and is equipped with a ball valve drain switch of appropriate size.

[0013] In combination with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions to be protected by the utility model are as follows:

[0014] First, the existing technical problems and technical progress solved by the utility model

[0015] 1. Reduced environmental dependence:

[0016] Problem: Traditional seedling raising methods rely on the natural environment, and factors such as temperature and rainfall affect the results of seedling raising.

[0017] Technical advantages: The device of the utility model can provide a stable cultivation environment and reduce the dependence on natural conditions.

[0018] 2. Hatching survival rate is improved:

[0019] Problem: The mortality rate of egg-bearing crabs is high, resulting in a high failure rate of seedling cultivation.

[0020] Technical advantages: The utility model solves the problem of seedling failure caused by the death of egg-bearing crabs through in vitro culture, and significantly improves the embryo hatching rate and survival rate.

[0021] 3. Adapt to the characteristics of crustacean embryo development:

[0022] Problem: Existing traditional fish embryo culture equipment is not suitable for crustaceans.

[0023] Technical advantages: The conical barrel design and rubber ring screen of the utility model are specially adapted to the sedimentation and suspension characteristics of the blue crab embryos in water, ensuring the effect of oxygenation and water exchange.

[0024] Second, the utility model proposes a production device for in vitro cultivation of Scylla pseudoburrowing embryos, the specific parameters of which are as follows:

[0025] 1) Conical barrel:

[0026] Opening diameter: 110cm

[0027] Bucket depth: 70cm

[0028] Bottom drainage hole: connected to the PVC water pipe, there is a row of aquaculture water switches at the end of the water pipe

[0029] 2) Rubber ring and screen:

[0030] Large rubber ring screen:

[0031] Diameter: 100cm

[0032] Screen mesh: 60 mesh

[0033] Small rubber ring screen:

[0034] Diameter: 10cm

[0035] Screen mesh: 60 mesh

[0036] 3) Cylindrical base:

[0037] Diameter: 70cm

[0038] Height: 60cm

[0039] 4) Oxygenation device:

[0040] Plastic pipe outer diameter: 10mm

[0041] Trachea inner diameter: 9mm

[0042] Air stone: 6cm diameter cake-shaped air stone

[0043] Equipped with a gas regulating valve of appropriate size

[0044] 5) Drain pipe:

[0045] PVC drain pipe diameter: 32mm

[0046] Equipped with a ball valve drain switch of appropriate size

[0047] Solve the technical problems existing in the existing technology:

[0048] 1) Embryo sinking and floating problems:

[0049] Existing in vitro culture devices for mud crab embryos often cause the embryos to sink to the bottom or float, resulting in embryo hypoxia or damage. The utility model can prevent the embryos from sinking to the bottom and floating by designing two rubber ring screens with different diameters, effectively keeping the embryos suspended in the culture water to ensure their normal development.

[0050] 2) Oxygen supply problem:

[0051] In traditional embryo culture devices, insufficient or uneven oxygen supply leads to poor embryo development. The utility model provides an airway hole between the small rubber ring screen and the bottom drainage hole, and uses a pancake-shaped air stone and an air regulating valve to ensure that the embryo obtains sufficient and uniform oxygen supply during the culture process.

[0052] 3) Embryo loss during water change:

[0053] During the water change process, the embryos are often discharged due to water flow. The large rubber ring screen designed in the utility model not only prevents the embryos from being brought out of the water during inflation, but also prevents the embryos from being siphoned out during water change, thereby protecting the integrity of the embryos.

[0054] Significant technological advances achieved:

[0055] 1) Improved embryo survival rate and development quality:

[0056] Through fine design and parameter adjustment, the utility model device can effectively prevent the embryo from sinking and floating, and provide a uniform oxygen supply, thereby significantly improving the survival rate and development quality of the embryo.

[0057] 2) Easy operation and maintenance:

[0058] The utility model has a simple design and uses an easy-to-operate air regulating valve and a ball valve drain switch, which is convenient for users to adjust and maintain in actual operation.

[0059] 3) Low cost and wide applicability:

[0060] The utility model adopts common materials such as PVC tubes, rubber rings, screens, etc., which reduces production costs. At the same time, the device has a simple structure and is suitable for embryo cultivation needs of different scales.

[0061] 4) Environmentally friendly:

[0062] The utility model has a reasonable design of the device, reduces embryo loss and pollutant discharge, helps protect the breeding environment, and achieves sustainable development.

[0063] The utility model solves the key problems existing in the prior art by means of ingenious structural design and parameter optimization, significantly improves the efficiency and effect of embryo cultivation, and provides a reliable technical guarantee for the cultivation of mud crabs.

[0064] Third, the expected benefits and commercial value after the technical solution of the utility model is transformed are: the number of eggs of the mud crab can reach an average of 1.5 million. Previously, after the egg-carrying crab died, its embryo was also discarded, which completely lost its application value. The embryos of the dead crab can be effectively hatched by using the device. The quality of the zoeae hatched during the period is no different from the quality of the juveniles hatched by the normal egg-carrying crab embryos, and both can be normally cultivated to commercial specifications. According to calculations, at least 1 million zoeae with good vitality can be obtained by using the device to in vitro hatch the embryos of the dead egg-carrying crabs. According to the calculation of the survival rate of artificial cultivation in the later stage, at least 150,000 to 200,000 crab seedlings of commercial specifications in the first stage can be obtained. The commodity price of each crab seedling is about 0.25 yuan. Finally, the device can save the economic losses of about 37,500 to 50,000 yuan.

[0065] The technical solution of the utility model fills the technical gap in the industry at home and abroad: At present, the in vitro culture of shrimp and crab embryos is at the basic experimental stage, and the research on the in vitro culture of embryos of blue crabs with poor vitality or dead eggs, and even normal crabs, has been blank. The device can culture a large number of embryos of blue crabs with good vitality, poor vitality or dead in vitro, so that it can produce crab seedlings for production practice in batches, and overcome the technical difficulty of not being able to obtain zoeae for production using in vitro culture technology in the past.

[0066] The technical solution of the utility model overcomes the technical prejudice: the blue crab embryo is fixed on the mother's belly navel through the bristles. The previous concept is that the mother crab can transmit nutrients or hormones to its embryo through the bristles to ensure the normal development of the embryo, and the embryo will die once it leaves the mother. The device can be used to hatch a large number of isolated blue crab embryos into zoeae, overcoming the technical prejudice that blue crab embryos need to obtain nutrients or hormones from the mother through the bristles during development. BRIEF DESCRIPTION OF THE DRAWINGS

[0067] Figure 1 It is a schematic diagram of a production device for in vitro cultivation of Scylla pseudoburrowing embryos provided in an embodiment of the utility model;

[0068] Figure 2 It is a schematic diagram of the survival rate of in vitro culture of blue crab embryos at different developmental stages provided by the embodiment of the utility model;

[0069] In the picture: 1. Conical bucket; 2. Handle; 3. Large rubber ring screen; 4. Small rubber ring screen; 5. Plastic pipe; 6. Air pipe; 7. Air regulating valve; 8. Drain switch; 9. PVC water pipe; 10. Cylindrical base; 11. Air stone. DETAILED DESCRIPTION

[0070] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail in combination with the embodiments below. It should be understood that the specific embodiments described here are only used to explain the utility model, and are not used to limit the utility model.

[0071] The utility model provides a production device for in vitro cultivation of Scylla pseudoburrowing embryos, which is composed of a conical barrel 1, a handle 2, a cylindrical base 10, a drain pipe and an oxygenating device. The bottom of the conical barrel 1 equipped with the handle 2 is provided with a drain hole, the bottom of the drain hole is connected to a PVC water pipe 9, and a row of aquaculture water switches are provided at the end of the PVC water pipe 9; two rubber ring screens with different diameters made of rubber rings and screens are inlaid at different positions of the conical barrel 1, a small rubber ring screen 4 located at the bottom can prevent the in vitro embryos of Scylla pseudoburrowing from sinking to the bottom of the conical barrel 1, and a large rubber ring screen 3 located at the upper part can prevent the embryos from being taken out of the water and adhering to the barrel wall of the conical barrel 1 during inflation, and can also prevent the in vitro embryos from being siphoned out during water change; the tracheal holes located at the small rubber ring screen 4 and the bottom drain hole can ensure the oxygen required by the embryos during the cultivation process, and the embryos are suspended in the aquaculture water by adjusting the air valve switch.

[0072] The cone barrel 1 has an opening diameter of 110 cm and a barrel depth of 70 cm.

[0073] The cylindrical base 10 has a diameter of 70 cm and a height of 60 cm.

[0074] The large rubber ring screen 3 has a diameter of 100 cm and a screen mesh number of 60 meshes.

[0075] The diameter of the small rubber ring screen 4 is 10 cm, and the screen mesh number is 60 meshes.

[0076] The plastic tube 5 has an outer diameter of 10 mm; the air pipe 6 has an inner diameter of 9 mm, and is equipped with an air regulating valve 7 of a suitable size, and an air stone 11 is a cake-shaped air stone with a diameter of 6 cm.

[0077] The PVC drain pipe has a diameter of 32 mm and is equipped with a ball valve drain switch 8 of appropriate size.

[0078] How to do it:

[0079] 1. Embryo disinfection

[0080] Use forceps to carefully peel the embryos from the belly button of the brooding crab. After peeling, soak the embryos in a 2ppm potassium permanganate solution for 10 minutes for disinfection, and then transfer them to disinfected seawater without potassium permanganate for cleaning.

[0081] 2. Disinfection of the Cultivation Device

[0082] The air stone 11, air pipe 6, conical barrel 1, assembled rubber ring screen, etc. of the cultivation device are soaked and disinfected with 10ppm potassium permanganate for 1 hour, and then cleaned with seawater. After cleaning, the inflation device, drainage device, screen, etc. are assembled on the conical barrel 1, and the assembled cultivation device is placed on the cylindrical base 10. Then, disinfected seawater with a salinity of about 25‰ is injected from the opening position of the conical barrel 1 until the water level is 20cm higher than the large rubber ring screen.

[0083] 3. Daily management during embryo in vitro culture

[0084] After hand disinfection, remove the large rubber ring screen 3 in the conical barrel 1, place the sterilized in vitro embryo in the conical barrel 1, and put the large rubber ring screen 3 back into the conical barrel 1 after it settles. Then open the inflation valve, adjust the air intake size according to the suspended state of the embryo in the water body, so that the embryo is suspended in the water. Siphon a certain amount of culture seawater from the bottom of the small rubber ring screen 4 every morning, and then slowly add fresh sterilized seawater from the top of the conical barrel 1. During the culture process, keep the temperature of the workshop where the culture barrel is placed constant at about 28 degrees.

[0085] 4. Embryo in vitro hatching cycle and hatching rate

[0086] By using the device of the utility model, the period for hatching the gastrula stage embryos of the mud crab into the I-stage zoea is 8 days, and the hatching rate is about 53%; the embryos in the compound eye formation stage are cultured in vitro for 3 days, and 87% of the embryos can hatch the I-stage zoea with good vitality; the period for hatching the intramembranous zoea embryos of the mud crab into the I-stage zoea is 1 day, and the hatching rate is about 98%.

[0087] Statistics were collected after in vitro culture of blue crab embryos at different developmental stages. The results showed that when the device was used to in vitro culture embryos at the gastrula stage for 8 days, 53% of the embryos could hatch into I-stage zoea with good vitality; when the embryos at the compound eye formation stage were in vitro cultured for 3 days, 87% of the embryos could hatch into I-stage zoea with good vitality; when the embryos at the intramembranous zoea stage were in vitro cultured for 1 day, 98% of the embryos could hatch into I-stage zoea with good vitality. The specific situation is shown in the table below:

[0088] Table 1. In vitro culture of blue crab embryos at different stages

[0089]

[0090] In the description of the present invention, unless otherwise specified, "multiple" means two or more; the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0091] The above description is only a specific implementation method of the present invention, but the protection scope of the present invention is not limited thereto. Any modification, equivalent substitution and improvement made by any technician familiar with the technical field within the technical scope disclosed by the present invention and within the spirit and principle of the present invention shall be covered by the protection scope of the present invention.

Claims

1. A production device for in vitro cultivation of Scylla scylla embryos, characterized in that: It consists of a conical barrel, a rubber ring, a screen, a handle, and a cylindrical base. 、 The utility model is composed of a drainage pipe and an oxygenating device. A drainage hole is arranged at the bottom of a conical barrel equipped with a handle. A PVC water pipe is connected to the bottom of the drainage hole. A row of aquaculture water switches are arranged at the end of the PVC water pipe. Two rubber ring screens with different diameters made of rubber rings and screens are inlaid at different positions of the conical barrel. The small rubber ring screen located at the bottom can prevent the in vitro embryos of mud crabs from sinking to the bottom of the conical barrel. The large rubber ring screen located at the upper part can prevent the embryos from being brought out of the water and adhering to the wall of the conical barrel during inflation, and can also prevent the in vitro embryos from being siphoned out during water change. The tracheal holes located at the small rubber ring screen and the drainage hole at the bottom can ensure the oxygen required by the embryos during the cultivation process, and the embryos can be suspended in the aquaculture water by adjusting the air valve switch.

2. The production device for in vitro cultivation of Scylla scylla embryos according to claim 1, characterized in that: The opening diameter of the conical barrel is 110cm and the barrel depth is 70cm.

3. The production device for in vitro cultivation of Scylla scylla embryos according to claim 1, characterized in that: The cylindrical base is 70cm in diameter and 60cm high.

4. The production device for in vitro cultivation of Scylla pseudoburrowing embryos according to claim 1, characterized in that: The diameter of the large rubber ring screen is 100cm and the mesh size is 60 meshes.

5. The production device for in vitro cultivation of Scylla scylla embryos according to claim 1, characterized in that: The diameter of the small rubber ring screen is 10cm and the mesh size is 60 meshes.

6. The production device for in vitro cultivation of Scylla scylla embryos according to claim 1, characterized in that: The outer diameter of the plastic tube is 10mm; the inner diameter of the air pipe is 9mm, and it is equipped with an air regulating valve of appropriate size and a cake-shaped air stone with a diameter of 6cm.

7. The production device for in vitro culture of Scylla scylla embryos according to claim 1, characterized in that: The PVC drain pipe has a diameter of 32mm and is equipped with a ball valve drain switch of appropriate size.

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