Drainage water tank device suitable for cell factory
By designing a drainage tank device for cell factories, the combination of diversion base plate and pump is used to solve the problem of waste liquid residue and equipment during cell factories discharge, and rapid and residual-free drainage is achieved, and production efficiency and product quality are improved.
Patent Information
- Application Number
- CN202421912111.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-07
- Publication Date
- 2025-06-06
- Estimated Expiration
- 2034-08-07
AI Technical Summary
The prior art has problems such as waste liquid residue, expensive equipment and complex operation, and slow discharge speed during the discharge process of cell factories, which affect production efficiency and product quality.
A liquid drainage tank device is designed, including the tank body part, the flow guide base, the liquid drain port, the support base and the connecting pipe. Through the inclined design of the flow guide base and the assistance of the pump, rapid and residual waste discharge is achieved.
The device overcomes the problems of waste liquid residue and expensive equipment, is simple to operate and fast discharge speed, reduces the risks of cell factory damage and impurity residue, and improves production efficiency and product quality.
Smart Images
Figure CN222949152U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the field of vaccine production, and more specifically, relates to a drainage trough device suitable for a cell factory. Background Art
[0002] Drainage is a key operation in the preparation of vaccines using cell factories. In the current large-scale vaccine production, there are two main ways to drain cell factories, namely vacuum pumping and using fully automatic or semi-automatic cell factory equipment to tilt the cell factory and drain through pipes.
[0003] Vacuum extraction method, because the cell factory is made of polypropylene, and has a tight structure, poor air permeability, and the sealing interface is a physical seal or glue seal, which is very easy to crack after the internal vacuum is evacuated. Due to its special material and structure, the vacuum extraction operation has poor fault tolerance, and the cell factory is easy to burst during the operation, which can easily cause the cultured cells to be contaminated; the surface of the cultured cells in the cell factory is attached with liquid, and it is not easy to extract it completely using only vacuum extraction, which can easily lead to excessive residues of impurities (including digestive fluid, bovine serum, antibiotics, etc.) on the surface of the cultured cells; and relevant literature shows that vacuum extraction can easily cause damage to the cultured cells in the cell factory.
[0004] The cell factory is tilted by using fully automatic or semi-automatic cell factory equipment, and the liquid is drained through the pipe. The tilted liquid can drain the liquid and impurities attached to the cell surface without damaging the cells, and effectively avoid the problem of easy damage to the cell factory after vacuuming. However, after using the cell factory equipment to tilt the cell factory, there are also certain problems in draining the liquid by gravity through the connected pipes. This method must use cell factory equipment, which is relatively expensive, and one device can only operate 1-2 cell factories at the same time. During large-scale production, multiple devices need to be prepared for operation at the same time. In addition, the cell factory equipment has high requirements for operating space and requires a larger clean area above Class C. The threshold for the use of such technology is high. After tilting, the cell factory needs to be connected to the pipe for drainage. Because the cell factory and the pipe connection port are connected by an internal plug, the inner diameter of the pipe connection port is smaller than the cell factory drainage port. The waste liquid cannot be completely discharged by gravity drainage, which is easy to cause residues at the connection position. As the cell factory is refilled, impurities will flow into the cell factory again. In addition, after tilting, gravity drainage through the pipe has a slow drainage speed, which affects production efficiency. Utility Model Content
[0005] The utility model aims to provide a drainage trough device, which comprises, from top to bottom, a trough body, a guide bottom plate and a drainage port at the bottom of the trough body, a support base and a connecting pipe, wherein the portion of the guide bottom plate connected to the drainage port is low in height, and the portion away from the drainage port is high in height, the drainage port is connected to the connecting pipe, and a card slot is provided on the trough body portion for fixing a cell factory so that the drainage port of the cell factory is suspended above the drainage trough device.
[0006] When the device is used, the waste liquid in the cell factory is directly discharged from the cell factory discharge port, and there is no area where the solution is retained, which overcomes the disadvantage of the prior art that the waste liquid is easily retained at the pipe connection port. The design is simple, the cost is low, the part in contact with the waste liquid is detachable, easy to sterilize, easy to operate, and the discharge speed is fast, which will not cause damage to the cell factory. When it is used to discharge waste liquid from the cell factory, the residual impurities on the cell surface are lower.
[0007] In a first aspect of the utility model, a drainage trough device is provided, which comprises, from top to bottom, a trough body portion, a guide bottom plate and a drainage port at the bottom of the trough body, a support base and a connecting pipe, wherein the portion of the guide bottom plate connected to the drainage port is low in height, and the portion away from the drainage port is high in height, the drainage port is connected to the connecting pipe, and a card slot is provided on the trough body portion for fixing the cell factory so that the drainage port of the cell factory is suspended above the drainage trough device.
[0008] In one or more embodiments, the trough body portion is rectangular, and the guide bottom plate includes a transverse guide bottom plate and a longitudinal guide bottom plate, which correspond to the long side and the short side of the trough body portion respectively.
[0009] In one or more embodiments, the inclination angle of the transverse guide bottom plate is 30°~40°, and the inclination angle of the longitudinal guide bottom plate is 15°~20°.
[0010] In one or more embodiments, the drain port is detachably connected to the connecting pipe.
[0011] In one or more embodiments, the drain port and the connecting pipe are detachably connected together via a pipe connector.
[0012] In one or more embodiments, the pipe connector is a pagoda connector.
[0013] In one or more embodiments, the drainage trough device further comprises a pump, and the pump is detachably connected to the connecting pipe.
[0014] In one or more embodiments, the pump is a peristaltic pump.
[0015] In one or more embodiments, the slots are an even number, which can fix the cell factory in a symmetrical manner.
[0016] In one or more embodiments, there are six slots, which can fix two cell factories symmetrically.
[0017] In one or more embodiments, the support base has an even number of support legs for stably supporting the trough body portion.
[0018] In one or more embodiments, the support base is bipedal, quadripedal, hexapedal, or octapedal.
[0019] Other aspects of the present invention will be apparent to those skilled in the art from the disclosure herein. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 , a three-dimensional view of the drain tank assembly when the pump is not connected.
[0021] Figure 2 , top view of the drainage trough device.
[0022] Figure 3 , Left side view of the drain sink assembly without the pump connected.
[0023] Figure 4 , Left side view of the drain sink assembly with the pump connected.
[0024] Figure 5 , a three-dimensional view of the drainage trough device after the cell factory is placed.
[0025] Figure 6 , A partial enlarged view of the drain outlet of the cell factory and the drain outlet of the drain sink device after the cell factory is placed.
[0026] Figure 7 , comparison of bovine serum albumin residue test results.
[0027] Figure 8 , comparison of titer test results.
[0028] Reference numerals:
[0029] 1. Drainage tank device;
[0030] 101, tank body;
[0031] 102. Longitudinal guide bottom plate;
[0032] 103. Horizontal guide bottom plate;
[0033] 104. Support base;
[0034] 105. Connecting pipes;
[0035] 106. Pipeline connector;
[0036] 107. Pump;
[0037] 108. Drainage port;
[0038] 109. Card slot;
[0039] 2. Cell factory device;
[0040] 201. Drainage outlet of cell factory. DETAILED DESCRIPTION
[0041] As shown in the present invention and claims, unless the context clearly indicates an exception, generally speaking, the terms "comprises" and "includes" only indicate the inclusion of clearly identified steps and elements, and these steps and elements do not constitute an exclusive list, and the method or device may also include other steps or elements.
[0042] When describing the embodiments of the present invention in detail, for the sake of convenience, the schematic diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.
[0043] The utility model provides a drainage trough device, which comprises, from top to bottom, a trough body part, a flow guide bottom plate and a drainage port at the bottom of the trough body, a supporting base and a connecting pipeline.
[0044] In a preferred embodiment of the present invention, the portion of the guide bottom plate connected to the drain port is low in height, and the portion away from the drain port is high in height. The liquid can be easily guided into the drain port by utilizing the height difference of the inclined guide bottom plate and gravity.
[0045] In a preferred embodiment of the present invention, the guide bottom plate is inclined on four sides and is concave toward the drainage outlet in the middle.
[0046] In a preferred embodiment of the present invention, the drainage trough device is in a rectangular shape, and the guide bottom plates are four triangular guide bottom plates, which are concave toward the drainage port in the middle.
[0047] In a preferred embodiment of the present invention, when the guide bottom plate is four triangular guide bottom plates, the triangular guide bottom plate whose bottom side corresponds to the long side (length) of the drainage trough device is a transverse guide bottom plate, and the triangular guide bottom plate whose bottom side corresponds to the short side (width) of the drainage trough device is a longitudinal guide bottom plate.
[0048] In a preferred embodiment of the present invention, the inclination angle of the transverse guide bottom plate is 30°, and the inclination angle of the longitudinal guide bottom plate is 15°.
[0049] In a preferred embodiment of the present invention, the support base may have two legs, four legs, six legs, eight legs or more legs, as long as it can stably support the trough body and allow the connecting pipe to be freely disassembled.
[0050] In a preferred embodiment of the present invention, the support base has four legs.
[0051] In a preferred embodiment of the present invention, the drain port and the connecting pipe are detachably connected together through a pipe connector. The detachable connection mode allows all parts that come into contact with the waste liquid during the production process to be detached and sterilized, and the detached parts are of suitable size, which is convenient for sterilization and transfer.
[0052] In a preferred embodiment of the present invention, the pipe connector is a pagoda connector.
[0053] In a preferred embodiment of the present invention, the connecting pipe is a hose.
[0054] In a preferred embodiment of the present invention, the drainage trough device may further include a pump, which is connected to the connecting pipe, thereby facilitating pumping out the liquid in the pipe.
[0055] In a preferred embodiment of the present invention, the pump is detachably connected to the connecting pipe. The detachable connection mode allows the pump to be sterilized after being disassembled, which is convenient for operation.
[0056] In a preferred embodiment of the present invention, the pump is a peristaltic pump, which can quickly guide the liquid in the pipeline, so that the waste liquid discharged from the cell factory is quickly introduced into the sewer pipe, and it is not easy to cause the waste liquid to accumulate.
[0057] In a preferred embodiment of the present invention, the material of the drainage trough device is a material resistant to sterilization and disinfectant corrosion, such as but not limited to stainless steel.
[0058] In a preferred embodiment of the present invention, a clamping groove is provided on the trough body of the drainage trough device so as to fix the cell factory.
[0059] In a preferred embodiment of the present invention, the number of the card slots is 2, 4, 6 or more, as long as the cell factory can be fixed.
[0060] In a preferred embodiment of the present invention, when the cell factory is placed on the card slot of the tank body of the drainage tank device, the cell factory is naturally tilted, which is beneficial to the discharge of waste liquid in the cell factory.
[0061] In a preferred embodiment of the present invention, when the cell factory is placed on the slot of the tank body of the drainage tank device, the drainage port of the cell factory is suspended above the drainage port of the drainage tank.
[0062] In a preferred embodiment of the present invention, the size of the tank portion is slightly larger than the size of 1, 2, 4 or more cell factories, so as to facilitate accommodating 1, 2, 4 or more cell factories.
[0063] In a preferred embodiment of the present invention, the size of the tank portion is slightly larger than the size of the two cell factories, and the length is just enough to allow the two cell factories to be placed opposite each other at an angle, thereby maximizing the use of space.
[0064] In a preferred embodiment of the present invention, there are six card slots, which can fix two cell factories symmetrically.
[0065] In a preferred embodiment of the present invention, when the cell factory is placed in the trough body, the cell factory is naturally tilted, which can quickly drain the waste liquid in the cell factory, minimize the residual impurities in the vaccine product, prevent the cell factory discharge port from contacting the inner wall of the trough, and reduce risks.
[0066] It should be understood that those skilled in the art can use one or more drainage trough devices according to the number of cell factories and the amount of waste liquid that needs to be discharged.
[0067] Those skilled in the art know that, usually, when using the cell factory method to prepare vaccines, waste liquids need to be discharged during the processes of replacing culture medium, cell digestion, virus inoculation, washing, etc., including the discharge of used cell culture medium, virus maintenance liquid waste liquid or washing liquid. The cell culture medium and virus maintenance liquid include but are not limited to serum, culture medium, antibiotics, etc.; the washing liquid includes but is not limited to PBS, inorganic salts and other ingredients. If the waste liquid remains at the pipe connection port, as the cell factory re-adds new virus maintenance liquid, serum or antibiotics will flow back into the cell factory, causing impurity residues to exceed the standard; as the culture expires, the residual impurities on the surface of the virus are washed away, and the serum-free culture medium is re-added during the culture process. The impurities that need to be washed and discharged will flow into the cell factory again, causing pollution and affecting the quality of vaccine production.
[0068] When the drainage trough device of the utility model is used, the cell factory is placed obliquely on the trough body of the drainage trough device, the drainage port of the cell factory is suspended above the drainage trough, and the pump switch is turned on. Since the longitudinal guide bottom plate and the transverse guide bottom plate at the bottom of the trough body are both placed obliquely, the waste liquid in the cell factory can easily flow into the bottom of the drainage trough by gravity, and the waste liquid is discharged through the drainage port connected to the pipeline. The pump is further used to quickly guide the liquid in the pipeline, so that the waste liquid discharged from the cell factory is quickly introduced into the sewer pipe, which is not easy to cause waste liquid accumulation.
[0069] The advantages of the utility model are:
[0070] (1) After the cell factory is placed, the drainage trough device of the utility model is directly suspended above the drainage trough. The waste culture fluid in the cell factory is directly discharged from the drainage trough of the cell factory, and there is no solution retention area, which overcomes the disadvantage of the prior art that waste fluid is easily left at the pipe connection port.
[0071] (2) The drainage trough device of the utility model is simple in design, low in cost, and the part in contact with the waste liquid is detachable, easy to sterilize, and convenient to operate;
[0072] (3) The drainage trough device of the utility model utilizes gravity and pump power at the same time, with a fast drainage speed, smooth flow of waste liquid, almost no accumulation of waste liquid, and no damage to the cell factory;
[0073] (4) When the drainage trough device of the utility model is applied to the discharge of waste liquid from a cell factory, the waste liquid is discharged without residue, and the residual impurities (including digestive fluid, bovine serum, antibiotics, etc.) on the cell surface during drainage are lower.
[0074] The present invention is further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer.
[0075] Embodiment 1. Drainage water tank device
[0076] A drainage trough device, the device includes from top to bottom: a rectangular trough body part 101, two longitudinal guide bottom plates 102, two transverse guide bottom plates 103 and a drainage port 108 at the bottom of the trough body part 101, a support base 104 and a connecting pipe 105. The longitudinal guide bottom plates 102 and the transverse guide bottom plates 103 are symmetrically arranged and are both concave toward the drainage port 108. A card slot 109 is arranged on the trough body part 101, so that the cell factory 2 can be fixed, so that the drainage port 201 of the cell factory is suspended above the drainage trough device. The drainage port 108 is detachably connected to the connecting pipe 105 through a pipe connector 106. The drainage trough device can also include a pump 107, which is detachably connected to the connecting pipe 105, so as to facilitate pumping out the liquid in the pipe. The support base can be two, four, six or more legs, as long as it can stably support the trough body 101 and allow the connecting pipe 105 to be freely disassembled. The slots 109 can be 2, 3, 4, 6 or more, as long as the cell factory can be fixed. The inclination angle of the longitudinal guide bottom plate 102 and the transverse guide bottom plate 103 can be arbitrary, as long as it is conducive to the flow of liquid into the drain port 108. Exemplary inclination angles are: the longitudinal guide bottom plate 102 is inclined at 15°, and the transverse guide bottom plate 103 is inclined at 30°.
[0077] Workflow: Such as Figure 5 and Figure 6 As shown, after the initial installation and sterilization, the cell factory is tilted and placed on the card slot 109 of the tank body part 101 of the drainage tank device, and the cell factory drainage port 201 is suspended above the drainage tank device. The switch of the pump 107 is turned on. Because the longitudinal guide bottom plate 102 and the transverse guide bottom plate 103 at the bottom of the tank body are tilted, the waste liquid in the cell factory can easily flow into the drainage port 108 by gravity, and the waste liquid is discharged through the connecting pipe 105. The pump 107 is further used to quickly guide the liquid in the pipeline, the drainage speed is fast, and the waste liquid flows smoothly, so that the waste liquid discharged from the cell factory is quickly introduced into the sewer pipe, which is not easy to cause waste liquid accumulation and will not cause damage to the cell factory. Before subsequent use, the connecting pipe 105, the pipe connector 106, and the pump 107 in contact with the waste liquid are disassembled, sterilized separately, and then assembled.
[0078] Example 2: Comparison between the drainage tank device and the vacuum extraction device
[0079] In this embodiment, the preparation of varicella attenuated live vaccine is taken as an example to compare the drainage tank device of the utility model with the vacuum pumping device to explore the residual impurities and viruses during drainage. The vacuum pumping device used in this embodiment is a DOA-P504-BN vacuum pump of Suzhou Jiashite Mechanical and Electrical Equipment Co., Ltd., which is installed and pumped according to the instructions of the device.
[0080] The human embryonic lung diploid cell MRC-5 strain (adherent cell) was inoculated with the attenuated varicella-zoster virus Oka strain, and the varicella live attenuated vaccine was prepared by the cell factory method. During the vaccine preparation process, the washing and changing operation procedure required the use of a drainage sink to drain the liquid. During the washing and changing operation, the virus maintenance liquid containing bovine serum was discarded, and the surface of the diseased cells containing the virus after 1 day of culture was washed twice with PBS. The discarded virus maintenance liquid and the waste liquid after washing were drained using a drainage sink device, and the virus maintenance liquid without bovine serum was replaced to continue the culture. After the culture expired, during the virus collection process, the adherent cells were separated from the cell factory using digestion fluid, and the diseased cells containing the virus were collected from the drainage port on the cell factory. The residual bovine serum albumin and the virus titer were detected, and a comparative analysis was performed. The vacuum extraction method was the control group, and the drainage sink device was the experimental group. A total of 3 repeated experiments were performed.
[0081] Bovine serum albumin detection method: Lowry method (Folin phenol method).
[0082] Virus titer detection method: plaque method.
[0083] The test results are shown in Table 1. The mean value of bovine serum albumin in the control group was 61.3±6.1 ng / ml, and that in the test group was 12.0±2.0 ng / ml. The mean value in the test group was much lower than that in the control group (e.g. Figure 7 The two-sample t-test results showed that there were significant differences between the control group and the experimental group.
[0084] The harvest titer test results showed that the mean value of the control group was 4.87±0.06 lgPFU / ml, and the mean value of the experimental group was 5.10±0.10 lgPFU / ml. The mean value of the experimental group was much higher than that of the control group (such as Figure 8 The two-sample t-test results showed that there were significant differences between the control group and the experimental group.
[0085] Table 1 Test results
[0086]
[0087] The results of bovine serum albumin residue and titer test showed that the impurity residue and final virus recovery rate of the drainage using the drainage trough device were significantly better than those using the vacuum extraction method.
[0088] The above-mentioned embodiments only express several implementation methods of the utility model, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the utility model, and these all belong to the protection scope of the utility model. Therefore, the protection scope of the patent of the utility model shall be subject to the attached claims. At the same time, all documents mentioned in the utility model are cited as references in this application, just as each document is cited as reference separately.
Claims
1. A drainage trough device suitable for a cell factory, characterized in that: The device comprises, from top to bottom, a trough body, a guide bottom plate and a drain port at the bottom of the trough body, a support base and a connecting pipe. The portion of the guide bottom plate connected to the drain port is low in height, and the portion away from the drain port is high in height. The drain port is connected to the connecting pipe, and a card slot is provided on the trough body for fixing the cell factory so that the cell factory drain port is suspended above the drain trough device.
2. The drainage trough device according to claim 1, characterized in that: The trough body part is rectangular, and the guide bottom plate includes a transverse guide bottom plate and a longitudinal guide bottom plate, which correspond to the long side and the short side of the trough body part respectively.
3. The drainage trough device according to claim 2, characterized in that: The inclination angle of the transverse guide bottom plate is 30°~40°, and the inclination angle of the longitudinal guide bottom plate is 15°~20°.
4. The drainage trough device according to claim 1, characterized in that: The liquid discharge port is detachably connected to the connecting pipe.
5. The drainage trough device according to claim 4, characterized in that: The liquid discharge port and the connecting pipe are detachably connected together via a pipe connector.
6. The drainage trough device according to claim 5, characterized in that: The pipeline connector is a pagoda connector.
7. The drainage trough device according to claim 1, characterized in that: The drainage trough device also includes a pump, and the pump is detachably connected to the connecting pipe.
8. The drainage trough device according to claim 7, characterized in that: The pump is a peristaltic pump.
9. The drainage trough device according to claim 1, characterized in that: The card slots are in an even number, and can fix the cell factories in a symmetrical manner.
10. The drainage trough device according to claim 1, characterized in that: The support base has an even number of support legs for stably supporting the trough body portion.