Porous homogeneous-phase fan-shaped disc structure

Abstract

The utility model provides a multi-hole homogeneous phase fan-shaped disc structure which comprises a disc seat and an adhesive film arranged on the disc seat, a flow channel is arranged on one side, opposite to a mixing cavity arranged on the disc seat, of the disc seat, and the flow channel is communicated with the mixing cavity; a plurality of groups of reaction holes are formed in the disc seat, each group of reaction holes comprises a first reaction hole and a second reaction hole, the first reaction hole is communicated with the second reaction hole through a capillary channel, and the first reaction hole is communicated with the flow channel; a first adhesive film is arranged on the back side of the disc seat and used for covering and sealing the bottom of the first reaction hole and the flow channel, and the second reaction hole is communicated with a first exhaust hole formed in the disc seat. According to the utility model, the reaction mode of a reagent in a detection hole in the prior art is changed, and two independent reaction opportunities are provided in a step-by-step mode to complete different reactions, so that the whole reaction is controllable, and the two-step reaction can be carried out in an optimal state to improve the accuracy of a detection result.

Description

Technical Field

[0001] The utility model discloses the technical field of a blood gas analysis reagent disc, in particular to a porous homogeneous sector-shaped disc structure. Background Art

[0002] Chemiluminescence is a light radiation phenomenon that accompanies a substance during a chemical reaction. It can be divided into direct luminescence and indirect luminescence. Direct luminescence is the simplest chemiluminescence reaction, which consists of two key steps: excitation and radiation. For example, when substances A and B undergo a chemical reaction to generate substance C, the energy released by the reaction is absorbed by the molecules of substance C and transitions to the excited state C. * , in the excited C * Light radiation is generated in the process of returning to the ground state. Here C* is the luminophore, and since C directly participates in the reaction, it is called direct chemiluminescence.

[0003] The invention with application number: CN202311491814.5 (hereinafter referred to as: prior art 1) discloses a microfluidic sector-shaped biochemical reagent tray assembly and a method of using the same, including a tray and a plurality of reagent trays, wherein a plurality of partitions are arranged in the tray, and the partitions divide the interior of the tray into a plurality of sector-shaped areas; the reagent tray is in a sector-shaped structure as a whole, and the shape of the reagent tray matches the sector-shaped area; the reagent tray is detachably mounted on the tray; a center hole is arranged in the middle of the tray, and the inner wall of the center hole has a spherical positioning groove for positioning with the positioning beads on the push rod mechanism in the tester; each sector-shaped area is provided at the bottom of the tray with a push rod for pushing up the water cup placed in the reagent tray. The invention can solve the technical problems that most of the reagent tray structures in the prior art are disc-shaped structures, and only one sample to be tested can be added to the reagent tray when testing, and multiple reagent trays need to be used for different test items and multiple tests are performed to complete the entire test process, and the test time is long.

[0004] Prior art 1 discloses a reagent disk, comprising a disk base with an overall fan-shaped structure and an adhesive film. The disk base is provided with a sample addition chamber, a buffer chamber, a sample quantitative chamber and a mixing chamber which are sequentially connected through capillary channels. A placement chamber and a liquid quantitative chamber are provided on the disk base near the sample addition chamber. The placement chamber is used to place a water cup. The placement chamber is connected to the liquid quantitative chamber, and the liquid quantitative chamber is connected to the mixing chamber. An annular flow channel is provided on the disk base near the edge of the disk base, and a plurality of detection holes connected to the annular flow channel are also provided on the disk base.

[0005] However, in actual use, the sample in the mixing chamber is mixed with the reagent and then directly enters the detection well for reaction. Since the immune reaction between antigen and antibody involves at least three substances, whether it is a sandwich method or a competitive method, although the one-step reaction that mixes the three together can use a simpler structure to obtain results faster, all reactions occur in the same detection well, and the order in which they occur in the detection well is uncontrollable. Utility Model Content

[0006] The purpose of the utility model is to provide a porous homogeneous sector disk structure, which changes the reaction mode of reagents in the detection hole in the prior art, and provides two separate reaction opportunities in a step-by-step manner to complete different reactions, so that the overall reaction is controllable, and both steps of the reaction can be carried out in the best state, thereby improving the accuracy of the detection results.

[0007] In order to solve the above technical problems, the technical solution adopted by the utility model is:

[0008] A porous homogeneous sector disk structure comprises a disk seat and an adhesive film arranged on the disk seat, a flow channel is arranged on the disk seat at a side opposite to a mixing chamber arranged on the disk seat, and the flow channel is communicated with the mixing chamber;

[0009] A plurality of reaction holes are arranged on the disc seat, each of which includes a first reaction hole and a second reaction hole, the first reaction hole and the second reaction hole are connected through a capillary channel, and the first reaction hole is connected with the flow channel;

[0010] A first adhesive film is arranged on the back side of the disc seat, and the first adhesive film is used to cover and seal the bottom of the first reaction hole and the flow channel. The second reaction hole is connected to the first exhaust hole arranged on the disc seat.

[0011] Wherein, a stirring unit is arranged in the mixing chamber.

[0012] Wherein, the stirring unit is a baffle arranged on the side wall and the bottom of the mixing chamber.

[0013] Furthermore, there are several baffles with different heights.

[0014] Wherein, a liquid guiding arc surface is arranged on the baffle bar.

[0015] The stirring unit is a baffle column arranged inside the mixing chamber, and the baffle column is vertical to the bottom of the mixing chamber.

[0016] Furthermore, the cross section of the barrier column is circular, elliptical or square.

[0017] Preferably, a diluent transition chamber is also provided on the disc seat, and the diluent transition chamber is used to connect with the diluent placement chamber and the diluent transition chamber provided on the disc seat.

[0018] Compared with the prior art, the utility model has the following beneficial effects:

[0019] Compared with the prior art, the utility model mainly realizes a step-by-step reaction at the detection hole; after the sample and the reagent are fully mixed in the mixing chamber, the mixed sample enters the flow channel and then enters the first reaction hole in sequence, and performs a primary reaction in the first reaction hole. After the reaction is completed, the sample enters the second reaction hole for a secondary reaction, thus achieving the purpose of step-by-step reaction. Both steps react independently, making the overall reaction system controllable, and being able to use different buffer systems for the two reactions, so that both steps of the reaction are carried out in the best state, thereby ensuring a sufficient reaction at the second reaction hole, thereby improving the accuracy of the final detection result. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solution of the implementation mode of the utility model, the drawings required for use in the implementation mode will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 This is one of the schematic diagrams of the structure of the utility model.

[0022] Figure 2 This is the second schematic diagram of the structure of the utility model.

[0023] Figure 3 For this utility model Figure 1 main view.

[0024] Reference numerals:

[0025] 101 disk base, 102 capillary channel, 103 sample adding chamber, 104 sample buffer chamber, 105 sample quantitative chamber, 106 mixing chamber, 107 blood cell collection chamber, 108 diluent placement chamber, 109 diluent quantitative chamber, 110 flow channel, 111 reaction hole, 112 first reaction hole, 113 second reaction hole, 114 baffle, 115 diluent transition chamber. DETAILED DESCRIPTION

[0026] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the embodiments of the present invention. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0027] In the description of the embodiments of the present invention, it should be understood that the terms "length", "vertical", "horizontal", "top", "bottom", etc. indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present invention.

[0028] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the embodiments of the present utility model, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0029] In the embodiments of the present utility model, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or a communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present utility model can be understood according to specific circumstances.

[0030] In the embodiments of the present utility model, unless otherwise clearly specified and limited, the first feature being "above" or "below" the second feature may include the first and second features being in direct contact, or may include the first and second features not being in direct contact but being in contact through another feature between them. Moreover, the first feature being "above", "above" and "above" the second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0031] The disclosure below provides many different embodiments or examples for realizing different structures of the embodiments of the present utility model. In order to simplify the disclosure of the embodiments of the present utility model, the components and settings of specific examples are described below. Of course, they are only examples, and the purpose is not to limit the embodiments of the present utility model. In addition, the embodiments of the present utility model can repeat reference numbers and / or reference letters in different examples, and this repetition is for the purpose of simplification and clarity, and does not itself indicate the relationship between the various embodiments and / or settings discussed.

[0032] The embodiments of the present utility model are described in detail below with reference to the accompanying drawings.

[0033] See also Figure 1-Figure 3 This embodiment discloses a sector disk structure, specifically a porous homogeneous sector disk structure, for homogeneous chemiluminescence detection; comprising a disk base 101 and an adhesive film arranged on the disk base 101, the disk base 101 is provided with a sample adding chamber 103, a sample buffer chamber 104 (sample transition chamber), a sample quantitative chamber 105 and a mixing chamber 106 which are sequentially connected through a capillary channel 102, and the sample quantitative chamber 105 is also connected to a waste liquid chamber, specifically a blood cell collection chamber 107;

[0034] A diluent placement chamber 108 and a diluent quantitative chamber 109 are provided on the tray base 101 near the sample adding chamber 103. The diluent placement chamber 108 is used to place a water cup. The diluent placement chamber 108 is connected to the diluent quantitative chamber 109, and the diluent quantitative chamber 109 is connected to the mixing chamber 106.

[0035] A flow channel 110 is provided on the disc base 101 on the side opposite to the mixing chamber 106 provided on the disc base 101 , and the flow channel 110 is communicated with the mixing chamber 106 ;

[0036] The disc base 101 is provided with a plurality of groups of reaction holes 111, each group of reaction holes 111 includes a first reaction hole 112 and a second reaction hole 113, the first reaction hole 112 and the second reaction hole 113 are connected through a capillary channel, and the first reaction hole 112 is connected to the flow channel 110;

[0037] A first adhesive film is disposed on the back side of the disc base 101 , and the first adhesive film is used to cover and seal the bottom of the first reaction hole 112 and the flow channel 110 . The second reaction hole 113 is connected to a first exhaust hole disposed on the disc base 101 .

[0038] In actual use, the first adhesive film covers the bottom of the first reaction hole 112 and the flow channel 110 to avoid leakage.

[0039] In actual use, different reaction substances are arranged in the first reaction hole 112 and the second reaction hole 113 according to detection requirements.

[0040] In this embodiment, there are 6 groups of reaction holes 111 .

[0041] Compared with the prior art, the utility model mainly realizes a step-by-step reaction at the detection hole; after the sample and the reagent are fully mixed in the mixing chamber 106, the mixed sample enters the flow channel 110 and then enters the first reaction hole 112 in turn, and a primary reaction is carried out in the first reaction hole 112. After the reaction is completed, the sample enters the second reaction hole 113 for a secondary reaction, thereby achieving the purpose of step-by-step reaction. The two steps are reacted independently so that the overall reaction system is controllable, and different buffer systems can be used for the two reactions, so that the two-step reactions are carried out in the best state, so as to ensure that a sufficient reaction is carried out in the second reaction hole 113, so as to improve the accuracy of the final detection result.

[0042] The present application mainly involves improvements to the structure of the reaction holes 111 of the reagent disk. If other structures are not specifically described, the other specific structures can refer to the reagent disk structure described in the prior art 1.

[0043] Furthermore, in some embodiments, a stirring unit is disposed in the mixing chamber 106 , and the stirring unit is a baffle 114 disposed on the side wall and the bottom of the mixing chamber 106 .

[0044] The baffle 114 can be provided to stir the solution in the mixing chamber 106 so that the solution in the mixing chamber 106 can be mixed more evenly.

[0045] In actual use, there are several baffles 114 with different heights to improve the mixing effect.

[0046] The baffle bar 114 is provided with a liquid-guiding curved surface, so that the liquid flows more smoothly.

[0047] As an option, in some specific implementation cases, the stirring unit is a baffle column arranged inside the mixing chamber 106, and the baffle column is perpendicular to the bottom of the mixing chamber 106. The setting of the baffle column can effectively improve the mixing effect.

[0048] In actual use, the cross section of the barrier column is circular, elliptical or square.

[0049] The disc base 101 is also provided with a diluent transition chamber 115, which is used to connect with the diluent placement chamber 108 and the diluent transition chamber 115 provided on the disc base 101. The provided diluent transition chamber 115 can realize the transition of the diluent.

[0050] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications falling within the scope of the present invention.

[0051] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. It should be pointed out that any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A porous homogeneous sector disk structure, comprising a disk seat and an adhesive film disposed on the disk seat, characterized in that: A flow channel is provided on the disc seat at a side opposite to the mixing chamber provided on the disc seat, and the flow channel is communicated with the mixing chamber; A plurality of reaction holes are arranged on the disc seat, each of which includes a first reaction hole and a second reaction hole, the first reaction hole and the second reaction hole are connected through a capillary channel, and the first reaction hole is connected with the flow channel; A first adhesive film is arranged on the back side of the disc seat, and the first adhesive film is used to cover and seal the bottom of the first reaction hole and the flow channel. The second reaction hole is connected to the first exhaust hole arranged on the disc seat.

2. A porous homogeneous sector disk structure according to claim 1, characterized in that: A stirring unit is arranged in the mixing chamber.

3. A porous homogeneous sector disk structure according to claim 2, characterized in that: The stirring unit is a baffle arranged on the side wall and the bottom of the mixing chamber.

4. The porous homogeneous sector disk structure according to claim 3, characterized in that: There are several baffles with different heights.

5. A porous homogeneous sector disk structure according to claim 2 or 4, characterized in that: A liquid guiding cambered surface is arranged on the baffle bar.

6. The porous homogeneous sector disk structure according to claim 2, characterized in that: The stirring unit is a baffle column arranged inside the mixing chamber, and the baffle column is vertical to the bottom of the mixing chamber.

7. The porous homogeneous sector disk structure according to claim 6, characterized in that: The cross section of the barrier column is circular, oval or square.

8. The porous homogeneous sector disk structure according to claim 7, characterized in that: The disc seat is also provided with a diluent transition chamber, which is used to connect with the diluent placement chamber and the diluent transition chamber provided on the disc seat.

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