Pleione bulbocodioides pseudobulb tissue culture device

Abstract

The utility model relates to the technical field of pleione bulbocodioides cultivation, and discloses a pleione bulbocodioides pseudobulb tissue culture cultivation device which comprises a cultivation box and a pleione bulbocodioides pseudobulb cuttage body, a water storage tank is arranged in the cultivation box in a connected mode, a plurality of cultivation holes communicated with the water storage tank are formed in the portion, above the water storage tank, of the cultivation box, and the pleione bulbocodioides pseudobulb cuttage body is arranged in the cultivation box. The bottom end of the cultivation hole is connected with a water filtering plate, the water filtering plate is provided with a foundation soil layer, the top of the cultivation hole is detachably connected with a retainer, and the retainer is connected with a limiting piece which can limit and retain the cuttage body and prevent the cuttage body from lodging. Compared with the prior art, the flowerpot has the advantages that water seepage can be conducted through the water filtering plate when watering is too much, water storage can be conducted through the water storage tank when watering is too little, then the capillary assembly is matched for follow-up use, and the management burden is reduced; the limiting piece can straighten the cuttage body to prevent lodging, and the cultivation effect is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of Pleione bulbocodioides cultivation, in particular to a Pleione bulbocodioides pseudobulb tissue culture cultivation device. Background Technique

[0002] There are two ways to reproduce Pleione bulbocodioides plants: asexual reproduction and sexual reproduction. Sexual reproduction is through the flowering and fruiting of Pleione bulbocodioides orchids, and reproduction depends on the seeds in the fruit. Asexual reproduction is that the orchid plants reproduce through the tillering of the bud points on the pseudobulbs. The tissue culture of Pleione bulbocodioides pseudobulbs is to cut the part of the tissue with new buds on the Pleione bulbocodioides pseudobulbs and cultivate it by artificial intervention by cutting and inserting it on the nutrient medium, so that it develops into a new individual in a short time.

[0003] Pleione bulbocodioides likes a warm, humid and semi-shady environment. During the tissue culture process of the existing Pleione bulbocodioides pseudobulbs, when too much water is poured, the cutting body is soaked in water for a long time, which is easy to cause rot. Or when less water is poured, the water flow cannot moisten the base soil layer when it reaches the bottom of the cultivation hole, thereby reducing the water absorption of the cutting body and affecting the cultivation effect. In addition, some cutting bodies are inclined during cutting and then fall down, which will also affect the cultivation effect. Content of the Utility Model

[0004] I. Technical Problems to be Solved

[0005] The technical problems to be solved by the utility model are that when too much water is poured, the cutting body is soaked and rots; when less water is poured, the water absorption requirement is not met and the cultivation effect is affected; when the cutting body is inclined, it is easy to fall down, which will also affect the cultivation effect.

[0006] II. Technical Solution

[0007] To solve the above technical problems, the technical solution provided by the utility model is: a Pleione bulbocodioides pseudobulb tissue culture cultivation device, including a cultivation box and a cutting body of Pleione bulbocodioides pseudobulbs. A water storage tank is connected and arranged inside the cultivation box. The cultivation box is provided with a plurality of cultivation holes communicating with the water storage tank above the water storage tank. A water filtering plate is connected to the bottom end of the cultivation hole. A base soil layer is arranged on the water filtering plate. A holding frame is detachably connected to the top of the cultivation hole. A limiting member is connected to the holding frame and can limit and hold the cutting body and prevent the cutting body from falling down.

[0008] Furthermore, connection grooves are respectively connected to both circumferential sides of the cultivation hole. The connection groove includes a through groove and a locking groove. Sliders are oppositely connected to the holding frame. The sliders are slidably matched with the connection groove.

[0009] Further, the cage is an annular cage with a U-shaped cross-section. The limiting member includes a sliding rod slidably connected to the top wall of the cage. A pressing plate is connected to the top end of the sliding rod. The bottom end of the sliding rod penetrates through the cage and a driving ring is connected to the inner side of the cage. A plurality of driven blocks are circumferentially and slidably connected to the inner bottom wall of the cage. A spring is connected between the driven block and the side wall of the cage. The other end of the driven block is connected with a clamping plate.

[0010] Further, a slide bar is connected to the bottom end of the driven block, and a chute slidably matched with the slide bar is oppositely connected to the cage.

[0011] Further, the top of the driven block is inclined downward in the direction close to the center of the cage, and the top wall of the driven block abuts against the bottom end of the driving ring.

[0012] Further, a capillary component is limited in the middle of the water filtering plate. The bottom end of the capillary component extends to the base soil layer, and the bottom end of the capillary component extends to the bottom of the water storage tank.

[0013] Further, an observation window is connected to the bottom of the water storage tank of the cultivation box.

[0014] III. Beneficial Effects

[0015] The advantages of the present utility model compared with the prior art are as follows:

[0016] Through the cooperative setting of the cultivation box, the water storage tank, the cultivation holes, the water filtering plate, the base soil layer, the cage and the limiting member, it is convenient to place the base soil layer on the water filtering plate. While facilitating the water filtering plate to support the base soil layer, it is also convenient for the excess irrigated water to be introduced into the water storage tank through the water filtering plate for storage; and the detachable setting of the cage facilitates the positioning of the cage, so as to limit and hold the cutting body through the limiting member to prevent it from lodging and affecting the cultivation effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a schematic structural diagram of a tissue culture cultivation device for pleione bulbocodioides var. emarginata pseudobulbs of the present utility model.

[0018] Figure 2 is a schematic main sectional structural diagram of a tissue culture cultivation device for pleione bulbocodioides var. emarginata pseudobulbs of the present utility model.

[0019] Figure 3 is Figure 2 an enlarged structural diagram of A in

[0020] Figure 4 is a schematic bottom sectional structural diagram of a tissue culture cultivation device for pleione bulbocodioides var. emarginata pseudobulbs of the present utility model.

[0021] As shown in the figure: 1. Cultivation box, 2. Water storage tank, 3. Cultivation holes, 4. Water filter plate, 5. Holder, 6. Connecting groove, 7. Through groove, 8. Locking groove, 9. Slide block, 10. Slide rod, 11. Pressing plate, 12. Driving ring, 13. Driven block, 14. Spring, 15. Clamping plate, 16. Slide bar, 17. Slide groove, 18. Capillary tube assembly, 19. Observation window. Detailed implementation method

[0022] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0023] Embodiment 1

[0024] Combined with the attached Figure 1 、 Figure 2 and Figure 4 , a tissue culture cultivation device for pleione bulbocodioides var. emarginata pseudobulbs, including a cultivation box 1 and a cutting body of pleione bulbocodioides var. emarginata pseudobulbs. A water storage tank 2 is connected and arranged inside the cultivation box 1. An observation window 19 is connected and arranged at the bottom of the water storage tank 2 in the cultivation box 1. A number of cultivation holes 3 communicating with the water storage tank 2 are opened above the water storage tank 2 in the cultivation box 1. A water filter plate 4 is connected and arranged at the bottom end of the cultivation holes 3. A base soil layer is arranged on the water filter plate 4. The base soil layer includes a gravel layer and a nutrient soil layer. The gravel layer is located below the nutrient soil layer, which is convenient for maintaining the nutrient soil layer when watering, reducing the possibility of clogging of the water filter plate, and at the same time facilitating the ventilation of the roots of the cutting body in the later stage. A holder 5 is detachably connected and arranged at the top of the cultivation holes 3. Connecting grooves 6 are respectively connected and arranged on both circumferential sides of the cultivation holes 3. The connecting grooves 6 include a through groove 7 and a locking groove 8. Slide blocks 9 are oppositely connected and arranged on the holder 5. The slide blocks 9 are slidably matched with the connecting grooves 6;

[0025] Combined with the attached drawings, a limiting member is connected to the cage 5, which can limit and hold the cutting body and prevent the cutting body from lodging. The cage 5 is an annular frame with a U-shaped cross-section. The limiting member includes a sliding rod 10 slidably connected to the top wall of the cage 5. A pressing plate 11 is connected to the top end of the sliding rod 10. The bottom end of the sliding rod 10 penetrates through the cage 5 and a driving ring 12 is connected to the inner side of the cage 5. A plurality of driven blocks 13 are circumferentially slidably connected to the inner bottom wall of the cage 5. A spring 14 is connected between the driven block 13 and the side wall of the cage 5. The other end of the driven block 13 is connected with a clamping plate 15. A sliding strip 16 is connected to the bottom end of the driven block 13. A chute 17 slidably matched with the sliding strip 16 is oppositely connected to the cage 5. The top of the driven block 13 is inclined downward along the direction close to the center of the cage 5. The top wall of the driven block 13 abuts against the bottom end of the driving ring 12.

[0026] Through the cooperation of the above structures, it is convenient to support the base soil layer through the water filter plate 4, and at the same time, it is also convenient to guide the excess water in the base soil layer into the water storage tank 2 through the water filter plate 4, avoiding the cutting body from rotting due to lack of oxygen when soaked in water and affecting the cultivation effect; the setting of the limiting member is convenient for straightening the cutting body and preventing it from lodging.

[0027] Embodiment 2

[0028] On the basis of Embodiment 1, combined with the attached Figure 2 , a capillary component 18 is limited in the middle of the water filter plate 4. The bottom end of the capillary component 18 extends to the nutrient soil layer of the base soil layer. The bottom end of the capillary component 18 extends to the bottom of the water storage tank 2. The capillary component 18 can be a cotton strip or a strip of rolled paper.

[0029] Through the above structure, it is convenient for the water in the water storage tank 2 to be fed back to the base soil layer through the capillary component 18, extending the watering interval and facilitating the management of the cutting body.

[0030] The specific usage method is as follows:

[0031] First, place the base soil layer on the water filter plate 4, and the upper limit of the base soil layer is lower than the bottom end height of the cage 5;

[0032] Then, slide the slider 9 on the cage 5 downward along the through groove 7 until the slider 9 abuts against the bottom of the through groove 7, and then rotate it to cooperate with the locking groove 8, so as to position the cage 5;

[0033] Next, by pressing the pressing plate 11, the pressing plate 11 drives the sliding rod 10 to drive the driving ring 12 downward. Since the top wall of the driven block 13 is of an inclined structure, as the driving ring 12 moves downward, the driven block 13 is squeezed to drive the clamping plate 15 to slide outward through the sliding fit between the sliding groove 17 and the sliding strip 16. At this time, the spring 14 is compressed and stores energy. The cutting body can be inserted through the clamping plates 15 and into the base soil layer. After releasing the pressing plate 11, under the reset action of the spring 14, the clamping plates 15 limit and clamp the cutting body circumferentially to prevent it from lodging.

[0034] Finally, the cutting body can be watered. The water flow is introduced into the base soil layer through the middle of the cage 5, and the excess water flow is introduced into the water storage tank 2 through the water filter plate 4 for storage, avoiding the cutting body from rotting due to being soaked in water for a long time. The capillary component 18 is provided to facilitate the transfer of the water flow in the water storage tank 2 to the base soil layer, avoiding the lack of water in the base soil layer from affecting the cultivation effect; when it is observed through the observation window 19 that the water storage in the water storage tank 2 is insufficient, the water replenishment operation can be carried out again.

[0035] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0036] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

[0037] The above describes the present invention and its implementation manners. This description is not restrictive. What is shown in the drawings is only one of the implementation manners of the present invention, and the actual structure is not limited thereto. Generally speaking, if those of ordinary skill in the art are inspired by it and design similar structural manners and embodiments without creative efforts without departing from the creative purpose of the present invention, they shall fall within the protection scope of the present invention.

Claims

1. A tissue culture cultivation device for pseudobulbs of Orchidaceae, comprising a cultivation box (1) and a cutting body of pseudobulbs of Orchidaceae, characterized in that: The cultivation box (1) is connected to a water storage tank (2), and the cultivation box (1) is provided with a plurality of cultivation holes (3) above the water storage tank (2) and in communication with the water storage tank (2). The bottom ends of the cultivation holes (3) are connected to a water filter plate (4), and a base soil layer is provided on the water filter plate (4). The tops of the cultivation holes (3) are detachably connected to a retaining frame (5), and the retaining frame (5) is connected to a limiting member capable of limiting and retaining a cutting body and preventing the cutting body from falling over.

2. A tissue culture and cultivation device for pseudobulbs of Orchidaceae according to claim 1, characterized in that: The cultivation hole (3) is connected to two circumferential sides thereof with connection grooves (6), the connection grooves (6) comprising a through groove (7) and a locking groove (8), and a slider (9) is relatively connected to the retaining frame (5), and the slider (9) is slidably matched with the connection groove (6).

3. A tissue culture and cultivation device for pseudobulbs of Orchidaceae according to claim 1, characterized in that: The retaining frame (5) is an annular frame with a U-shaped structural cross-section, and the limiting member includes a slide rod (10) slidably connected to the top wall of the retaining frame (5), the top end of the slide rod (10) is connected to a pressure plate (11), the bottom end of the slide rod (10) passes through the retaining frame (5) and is connected to a driving ring (12) on the inner side of the retaining frame (5), a plurality of driven blocks (13) are circumferentially slidably connected to the inner bottom wall of the retaining frame (5), a spring (14) is connected between the driven block (13) and the side wall of the retaining frame (5), and the other end of the driven block (13) is connected to a clamping plate (15).

4. A tissue culture and cultivation device for pseudobulbs of Orchidaceae according to claim 3, characterized in that: The bottom end of the driven block (13) is connected to a slide bar (16), and the retaining frame (5) is relatively connected to a slide groove (17) that slidably cooperates with the slide bar (16).

5. A tissue culture and cultivation device for pseudobulbs of Orchidaceae according to claim 3, characterized in that: The top of the driven block (13) is tilted downwards in a direction close to the center of the retaining frame (5), and the top wall of the driven block (13) abuts against the bottom end of the driving ring (12).

6. A tissue culture and cultivation device for pseudobulbs of Orchidaceae according to claim 1, characterized in that: A capillary assembly (18) is provided at a limited position in the middle of the water filter plate (4), the bottom end of the capillary assembly (18) extending to the base soil layer, and the bottom end of the capillary assembly (18) extending to the bottom of the water storage tank (2).

7. The device for tissue culture of pseudobulbs of Orchidaceae according to claim 1, characterized in that: The cultivation box (1) is connected to the bottom of the water storage tank (2) and is provided with an observation window (19).

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