Washing device for exosome purification

Abstract

The utility model discloses a washing device for exosome purification. The washing device comprises a liquid storage bottle, a liquid conveying pump, a washing assembly and a recycling assembly, the liquid storage bottle is connected with the washing assembly through the liquid conveying pump, and the washing assembly is connected with the recycling assembly through the recycling pipe. The washing assembly comprises a fixed frame, a movable frame, a first filtering assembly and a second filtering assembly; the fixed frame and the movable frame are detachably connected; a plurality of washing tanks are arranged in the fixed frame; the bottom of each washing tank is communicated with a liquid supply cavity at the bottom of the fixed frame through a liquid supply channel; the liquid supply cavity is connected with a liquid storage bottle through a liquid conveying pump and a liquid supply pipeline; a first filtering assembly is arranged at the lower part of the washing tank; a plurality of sleeves matched with the washing tanks are arranged on the movable frame; a second filter component is arranged at the bottom of the sleeve; a liquid discharging cavity is formed in the movable frame; a liquid discharge hole communicated with the liquid discharge cavity is formed in the movable frame in the sleeve; and a liquid discharging opening in one side of the liquid discharging cavity is connected with the recycling assembly through a recycling pipe. According to the exosome extraction device, the exosome extraction yield is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome purification, in particular to a washing device for exosome purification. Background Art

[0002] Exosomes refer to small membrane vesicles containing complex RNA and proteins. Currently, they specifically refer to discoid vesicles with a diameter of 40 - 100 nm, and various cells can secrete exosomes under normal and pathological conditions.

[0003] The existing exosome extraction uses plasma as raw material. After multiple centrifugations, the obtained supernatant is filtered and then ultracentrifuged again, and the obtained precipitate is washed with PBS. In the washing device of the existing technology, the loss of precipitate substances is caused by the impact of water flow. Therefore, it is necessary to design a washing device to improve the extraction rate of exosomes. Summary of the Utility Model

[0004] The technical problem to be solved by the utility model is to provide a washing device for exosome purification to improve the extraction rate of exosomes.

[0005] To solve the above technical problem, the utility model adopts the following technical solutions: A washing device for exosome purification includes a liquid storage bottle, an infusion pump, a washing component, and a recovery component; the liquid storage bottle is connected to the washing component through the infusion pump, and the washing component is connected to the recovery component through a recovery pipe;

[0006] The washing component includes a fixed frame, a movable frame, a first filtering component, and a second filtering component; the fixed frame and the movable frame are detachably connected; several washing grooves are arranged in the fixed frame; the bottom of each washing groove is communicated with a liquid supply cavity at the bottom of the fixed frame through a liquid supply channel; the liquid supply cavity is connected to the liquid storage bottle through the infusion pump and a liquid supply pipe; a first filtering component is arranged at the lower part of the washing groove; several sleeves matching the washing grooves are arranged on the movable frame; a second filtering component is arranged at the bottom of the sleeve; a liquid discharge cavity is arranged in the movable frame; a liquid discharge hole communicated with the liquid discharge cavity is arranged on the movable frame in the sleeve; a liquid discharge port on one side of the liquid discharge cavity is connected to the recovery component through a recovery pipe.

[0007] Further, a sealing ring is arranged on the outer periphery of the sleeve; a convex ring in contact with the sealing ring is arranged on the upper side wall of the washing groove.

[0008] Further, positioning holes are arranged at the four corners of the fixed frame; positioning columns are arranged at the four corners of the movable frame.

[0009] Further, the fixed frame and the movable frame are buckled.

[0010] Further, the fixed frame and the movable frame are connected by bolts.

[0011] Further, the recycling component is a recycling tank.

[0012] The beneficial effects of the present utility model are as follows:

[0013] 1) The present utility model sets a liquid storage bottle, an infusion pump, a washing component and a recycling component to achieve continuous flushing.

[0014] 2) The present utility model improves the washing component. By setting a first filtering component and a second filtering component, the exosomes are located between the first filtering component and the second filtering component, avoiding the loss of sediment due to the impact of water flow during flushing, and improving the extraction rate of exosomes. Description of the Drawings

[0015] The following further describes the present utility model in conjunction with the drawings and embodiments.

[0016] Figure 1 is a schematic structural diagram of the present utility model.

[0017] Figure 2 is a schematic structural diagram of the washing component. Detailed Embodiments

[0018] The following will clearly and completely describe the technical solutions of the present utility model through specific embodiments.

[0019] Refer to Figure 1 and Figure 2 , a washing device for purifying exosomes of the present utility model, comprising a liquid storage bottle 1, an infusion pump 2, a washing component 3 and a recycling component 4; the liquid storage bottle 1 is connected to the washing component 3 through the infusion pump 2, and the washing component 3 is connected to the recycling component 4 through a recycling pipe 5; the recycling component 4 is a recycling tank.

[0020] The washing component 3 of this embodiment includes a fixed frame 31, a movable frame 32, a first filtering component 33 and a second filtering component 34; the fixed frame 31 and the movable frame 32 are detachably connected; positioning holes are provided at the four corners of the fixed frame 31; positioning posts are provided at the four corners of the movable frame 32.

[0021] The fixed frame 31 and the movable frame 32 can be connected by snap-fastening.

[0022] The fixed frame 31 and the movable frame 32 can also be connected by bolts.

[0023] A number of washing tanks 35 are provided inside the fixed frame 31; the bottom of each washing tank 35 is communicated with a liquid supply chamber 37 at the bottom of the fixed frame 31 through a liquid supply channel 36; the liquid supply chamber 37 is connected to a liquid storage bottle 1 through an infusion pump 2 and a liquid supply pipeline; a first filtering assembly 33 is arranged at the lower part of the washing tank 35; a number of sleeves 38 matching the washing tanks 35 are arranged on the movable frame 32; a sealing ring 6 is arranged on the outer periphery of the sleeve 38; a convex ring 61 in contact with the sealing ring is arranged on the upper side wall of the washing tank 35. A second filtering assembly 34 is arranged at the bottom of the sleeve; a liquid discharge chamber 39 is arranged inside the movable frame 32; a liquid discharge hole 40 communicated with the liquid discharge chamber 39 is arranged on the movable frame 32 inside the sleeve; a liquid discharge port on one side of the liquid discharge chamber 39 is connected to a recovery assembly 4 through a recovery pipe 5.

[0024] Both the first filtering assembly 33 and the second filtering assembly 34 in this embodiment include a filter membrane and a filter frame for fixing the filter membrane, and the filter frame is installed inside the washing tank 35 or inside the sleeve. The filter membrane in this embodiment is used to protect exosomes and prevent exosomes from being filtered out through the filter membrane.

[0025] The working principle of this embodiment: Pour the stock solution to be washed into the washing tank 35, fix the movable frame 32 to the fixed frame 31, and the sleeve extends into the washing tank 35 and is located above the washing tank 35. At this time, the first filtering assembly 33 and the second filtering assembly 34 are respectively arranged above and below the washing tank 35. Pump the buffer solution into the liquid supply chamber 37, wash the stock solution to be washed in the washing tank from the washing tank 35, and the washed liquid is discharged from the liquid discharge chamber 39 of the movable frame 32.

[0026] The above description is only a preferred embodiment of the present invention and is not used to limit the present invention. Those skilled in the art can make various modifications or equivalent replacements within the essence and protection scope of the present invention, and such modifications or equivalent replacements should also be regarded as falling within the protection scope of the technical solution of the present invention.

Claims

1. A washing device for purifying exosomes, characterized in that: It comprises a liquid storage bottle (1), an infusion pump (2), a washing component (3) and a recovery component (4); the liquid storage bottle (1) is connected to the washing component (3) via the infusion pump (2), and the washing component (3) is connected to the recovery component (4) via a recovery pipe (5); The washing assembly (3) comprises a fixed frame (31), a movable frame (32), a first filter assembly (33), and a second filter assembly (34); the fixed frame (31) and the movable frame (32) are detachably connected; a plurality of washing troughs (35) are arranged in the fixed frame (31); the bottom of each washing trough (35) is connected to a liquid supply cavity (37) at the bottom of the fixed frame (31) through a liquid supply channel (36); the liquid supply cavity (37) is connected to a liquid storage bottle (1) through an infusion pump (2) and a liquid supply pipeline. ) connected; a first filter assembly (33) is arranged at the lower part of the washing tank (35); a plurality of sleeves (38) matching the washing tank (35) are arranged on the movable frame (32); a second filter assembly (34) is arranged at the bottom of the sleeve; a drainage cavity (39) is arranged in the movable frame (32); a drainage hole (40) communicating with the drainage cavity (39) is arranged on the movable frame (32) in the sleeve; a drainage port on one side of the drainage cavity (39) is connected to the recovery assembly (4) via a recovery pipe (5).

2. A washing device for purifying exosomes according to claim 1, characterized in that: A sealing ring (6) is provided on the outer periphery of the sleeve (38); and a convex ring (61) in contact with the sealing ring is provided on the upper side wall of the washing tank (35).

3. A washing device for purifying exosomes according to claim 1, characterized in that: Positioning holes are provided at the four corners of the fixed frame (31); and positioning columns are provided at the four corners of the movable frame (32).

4. A washing device for purifying exosomes according to claim 1, characterized in that: The fixed frame (31) and the movable frame (32) are buckled together.

5. A washing device for purifying exosomes according to claim 1, characterized in that: The fixed frame (31) and the movable frame (32) are connected by bolts.

6. A washing device for purifying exosomes according to claim 1, characterized in that: The recovery component (4) is a recovery tank.

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