Cell cleaning fluid sample adding device

Abstract

The utility model provides a cell cleaning fluid sample adding device and belongs to the technical field of sample adding devices. A sample adding area is arranged below a liquid storage area, the sample adding area and the liquid storage area are separated through a partition plate, quantitative cell cleaning fluid is added into a plurality of droppers at a time through a liquid transfer pump, a first hose and a second hose, and then cell culture fluid sample adding of culture dishes of a plurality of cell culture plates is achieved at a time; besides, a heat conduction layer and a heating device are arranged on the outer side of the liquid storage area to heat the liquid storage area, so that the cell cleaning liquid can be preheated in advance, and the cell cleaning liquid in the liquid storage area can be preheated in advance and then used for cleaning when a general cell culture digestion experiment or an FITC marking experiment is carried out.

Description

Technical Field

[0001] The utility model belongs to the technical field of sample adding devices, and particularly relates to a cell cleaning solution sample adding device. Background Art

[0002] The commonly used cell culture plates in the prior art are mainly used for culturing cells or measuring protein concentration. Cell culture plates are divided into flat-bottom and round-bottom according to the different shapes of the bottom, and the number of culture holes is 6, 12, 24, 48, 96, 384, 1536, etc. Among them, the 96-well cell culture plate is one of the commonly used specifications;

[0003] At present, for the cleaning of cell culture plates, the commonly used pipette can only perform single sample addition, that is, a pipette is used manually with a smearer once, and each time it is necessary to aspirate the sample from the reagent bottle and then add it to the reagent tube in the reaction plate, and so on until the addition is completed. This is not only time-consuming and laborious, but also has low personnel efficiency. In addition, when performing general cell culture digestion experiments or FITC labeling experiments, if PBS cell cleaning solution is used for cleaning, the cell cleaning solution needs to be preheated. Therefore, a cell cleaning solution sample adding device that can add multiple samples at one time and preheat the cell cleaning solution is needed. Summary of the Utility Model

[0004] The utility model aims at the problems in the prior art and provides a cell cleaning solution sample adding device.

[0005] In order to achieve the above object, the technical solution adopted in this application is as follows:

[0006] A cell cleaning solution sample adding device includes a housing; the housing includes a liquid storage area and a sample adding area; the liquid storage area is arranged above the sample adding area; the liquid storage area and the sample adding area are separated by a partition; a sample adding mechanism is arranged in the sample adding area;

[0007] The sample adding mechanism includes a dropper rack, droppers and a transfusion tube; the dropper rack is detachably installed on the bottom surface of the inner wall of the housing, and the dropper rack has droppers arranged in a rectangular array; the output end of the dropper passes through the inner wall of the bottom surface of the housing; the transfusion tube is arranged above the dropper; a cell cleaning solution inlet is arranged at the top of the transfusion tube, and the bottom outlet of the transfusion tube is communicated with the dropper;

[0008] A liquid transfer pump is fixedly installed on the top surface of the housing, the inlet of the liquid transfer pump is communicated with a first hose, and the other end of the first hose extends into the bottom of the liquid storage area; the outlet of the liquid transfer pump is communicated with a second hose, and the other end of the second hose is communicated with the top outlet of the transfusion tube.

[0009] Preferably, a heat conducting layer is wrapped on the outer side of the housing at the liquid storage area, and a heating device is fixedly installed on the left side surface of the heat conducting layer.

[0010] Preferably, first brackets are fixedly installed on the left and right sides of the housing at the sample loading area, and the height of the first brackets is higher than that of the sample loading area; a second bracket is fixedly installed on the bottom surface of the heating device.

[0011] Compared with the prior art, the advantages and positive effects of the present utility model are as follows:

[0012] In a cell cleaning solution sample loading device of the present utility model, a sample loading area is arranged below a liquid storage area, and the sample loading area and the liquid storage area are separated by a partition. A quantitative cell cleaning solution is added into a plurality of droppers at one time through a liquid transfer pump, a first hose and a second hose, so as to realize the sample loading of cell culture solutions in culture dishes of a plurality of cell culture plates at one time; in addition, a heat conducting layer and a heating device are arranged outside the liquid storage area to heat the liquid storage area, so that the cell cleaning solution can be preheated in advance. When performing a general cell culture digestion experiment or a FITC labeling experiment, the cell cleaning solution in the liquid storage area can be preheated first and then used for cleaning. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for describing the embodiments or the prior art. Obviously, the following drawings are some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0014] Figure 1 It is a schematic structural diagram of the present utility model;

[0015] Figure 2 It is a top view of the structure schematic diagram of the dropper rack;

[0016] In the above figures, 1. housing; 2. liquid storage area; 3. sample loading area; 4. partition; 5. dropper rack; 6. dropper; 7. infusion tube; 8. liquid transfer pump; 9. first hose; 10. second hose; 11. heat conducting layer; 12. heating device; 13. first bracket. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0017] In order to more clearly understand the above objects, features and advantages of the present utility model, the following further illustrates the present utility model with reference to the drawings and embodiments. It should be noted that, without conflict, the embodiments of the present application and the features in the embodiments can be combined with each other.

[0018] In the following description, many specific details are set forth in order to fully understand the present utility model. However, the present utility model can also be implemented in other ways different from those described herein. Therefore, the present utility model is not limited by the specific embodiments disclosed in the following specification.

[0019] Example 1, as shown in Figure 1 , Figure 2 A cell cleaning solution sampling device of the present application includes a housing 1; the housing 1 is a hollow cuboid; the housing 1 includes a liquid storage area 2 and a sampling area 3; the liquid storage area 2 is arranged above the sampling area 3; the liquid storage area 2 and the sampling area 3 are separated by a partition 4; the liquid storage area 2 stores the cell cleaning solution; a sampling mechanism is arranged in the sampling area 3.

[0020] The sampling mechanism includes a dropper rack 5, droppers 6 and an infusion tube 7; the dropper rack 5 is detachably installed on the inner bottom surface of the housing 1, and the dropper rack 5 has droppers 6 arranged in a rectangular array; in this embodiment, the arrangement of the droppers 6 in the rectangular array is eight rows and twelve columns, and a total of 96 droppers 6 are provided; the output end of the dropper 6 passes through the inner wall of the bottom surface of the housing 1; during use, the cleaning solution is dropped into the culture dishes of multiple cell culture plates at one time through the output end of the dropper 6; the infusion tube 7 is arranged above the dropper 6; the top of the infusion tube 7 is provided with an inlet for the cell cleaning solution, and the bottom outlet of the infusion tube 7 is communicated with the dropper 6.

[0021] A liquid transfer pump 8 is fixedly installed on the top surface of the housing 1, the inlet of the liquid transfer pump 8 is communicated with a first hose 9, and the other end of the first hose 9 extends into the bottom of the liquid storage area 2; specifically, the first hose 9 contacts the top surface of the partition 4; the outlet of the liquid transfer pump 8 is communicated with a second hose 10, and the other end of the second hose 10 passes through the housing 1 from the back surface of the housing 1 at the sampling area 3 and enters the sampling area 3 to be communicated with the top outlet of the infusion tube 7; during use, a quantitative cell cleaning solution is pumped into the infusion tube 7 by controlling the motor built in the liquid transfer pump 8, and then added into the dropper 6 through the infusion tube 7, and the cleaning solution of the cell culture plate is sampled through the dropper 6.

[0022] The outer side of the housing 1 at the liquid storage area 2 is wrapped with a heat conducting layer 11, and a heating device 12 is fixedly installed on the left side surface of the heat conducting layer 11; when performing a general cell culture digestion experiment or a FITC labeling experiment, the cell cleaning solution in the liquid storage area 2 can be preheated first and then used for cleaning.

[0023] First brackets 13 are fixedly installed on the left and right side surfaces of the housing 1 at the sampling area 3, and the height of the first brackets 13 is higher than the height of the sampling area 3; specifically, the first brackets 13 are arranged below the heat conducting layer 11, and the first brackets 13 are fixedly connected to the bottom surface of the heat conducting layer 11; during use, the cell culture plate is pushed into the position directly below the dropper 6 from the front or the back to sample the cleaning solution of the cell culture plate; a second bracket 14 is fixedly installed on the bottom surface of the heating device 12; the second bracket 14 has the same height as the first bracket 13.

[0024] The above are only the preferred embodiments of the present utility model, and do not limit the present utility model in other forms. Any person skilled in the art may use the technical content disclosed above to make changes or modifications into equivalent embodiments with equivalent changes and apply them to other fields. However, as long as it does not depart from the technical solution content of the present utility model, any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present utility model still belong to the protection scope of the technical solution of the present utility model.

Claims

1. A cell washing solution adding device, characterized in that: The invention comprises a shell (1); the shell (1) comprises a liquid storage area (2) and a sample adding area (3); the liquid storage area (2) is arranged above the sample adding area (3); the liquid storage area (2) and the sample adding area (3) are separated by a partition (4); and a sample adding mechanism is arranged in the sample adding area (3); The sample adding mechanism comprises a dropper rack (5), a dropper (6) and an infusion tube (7); the dropper rack (5) is detachably mounted on the bottom surface of the inner wall of the housing (1); the dropper rack (5) has droppers (6) in a rectangular array; the output end of the dropper (6) passes through the inner wall of the bottom surface of the housing (1); the infusion tube (7) is arranged above the dropper (6); a cell washing liquid inlet is arranged at the top of the infusion tube (7), and an outlet at the bottom of the infusion tube (7) is connected to the dropper (6); A liquid transfer pump (8) is fixedly mounted on the top surface of the housing (1); the inlet of the liquid transfer pump (8) is connected to a first hose (9), the other end of the first hose (9) extends into the bottom of the liquid storage area (2); the outlet of the liquid transfer pump (8) is connected to a second hose (10), the other end of the second hose (10) is connected to the top outlet of the infusion tube (7).

2. A cell washing solution adding device according to claim 1, characterized in that: The outer side surface of the shell (1) at the liquid storage area (2) is wrapped with a heat-conducting layer (11), and a heating device (12) is fixedly installed on the left side surface of the heat-conducting layer (11).

3. A cell washing solution adding device according to claim 2, characterized in that: A first bracket (13) is fixedly mounted on the left and right sides of the shell (1) at the sample loading area (3), and the height of the first bracket (13) is higher than the height of the sample loading area (3); and a second bracket (14) is fixedly mounted on the bottom surface of the heating device (12).

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