Nucleic acid purification device for umbilical cord mesenchymal stem cells

Abstract

The utility model relates to a nucleic acid purification device for umbilical cord mesenchymal stem cells, which comprises chambers which are wide at the upper part and narrow at the lower part in the width direction and are used for accommodating mesenchymal stem cell samples, a plurality of chambers are combined in an array form to jointly form an extraction part, and the extraction part can move in different processing parts. The two magnetic field parts with the magnetic force distributed in the staggered manner are arranged, so that the chambers can be alternately subjected to the magnetic force action in different directions, magnetic particles and nucleic acid can be more sufficiently combined or separated, and rapid, effective and automatic nucleic acid extraction and purification treatment of umbilical cord mesenchymal stem cells can be realized.

Description

Technical Field

[0001] The utility model relates to the field of biological technology devices, specifically to a device for nucleic acid purification, and more specifically to a device for nucleic acid purification of umbilical cord mesenchymal stem cells. Background Art

[0002] Umbilical cord mesenchymal stem cells are adult stem cells with multi-directional differentiation potential and are widely present in various tissues such as the umbilical cord. They have important application prospects in regenerative medicine, tissue engineering, and cell therapy. In order to study the gene expression changes of umbilical cord mesenchymal stem cells under different conditions, or to perform whole-genome sequencing, single nucleotide polymorphism (SNP) analysis, etc. on umbilical cord mesenchymal stem cells, the nucleic acid extraction and purification of umbilical cord mesenchymal stem cells is a key link. Although there are many nucleic acid purification devices in the prior art, the following technical problems still exist: First, when the number of cells is very limited, it is impossible to extract enough nucleic acid amount, resulting in low sensitivity; Second, during the nucleic acid extraction process, factors such as sample processing, reagent quality, and operation steps will affect the reliability of the extraction results, and this uncertainty will affect the repeatability of the experiment and the reliability of the results; Finally, when there are a large number of impurity components such as cell debris, proteins, fats, and microorganisms or pathogens in the sample, it is difficult to completely remove the impurities, resulting in low nucleic acid purity. In view of the above problems existing in the prior art, a device with high sensitivity is needed to extract and purify nucleic acids from umbilical cord mesenchymal stem cell samples, especially a device that can perform rapid, effective, and fully automated processing on complex samples. Summary of the Invention

[0003] In view of the above problems existing in the prior art, the utility model provides a device for nucleic acid purification of umbilical cord mesenchymal stem cells. The device is provided with a chamber for accommodating and purifying umbilical cord mesenchymal stem cells. A plurality of semi-circular grooves are arranged in parallel along the length direction at the bottom of the chamber. The plurality of chambers are combined in an array form to form an extraction part. The extraction part is arranged to be movable between a plurality of processing parts. The device is further provided with a vibration part, a first magnetic field part, a second magnetic field part, and a temperature control part. Magnetic tapes are distributed in a staggered manner between the first magnetic field part and the second magnetic field part. The magnetic tapes are arranged such that when the extraction part is located between the first magnetic field part and the second magnetic field part, the directions of the magnetic attraction received by the bottom of the chamber are opposite. Further, the chamber gradually narrows in width from top to bottom while remaining unchanged in length.

[0004] The present utility model is provided with a chamber having a shape that is wider at the top and narrower at the bottom in the width direction. On the one hand, it can help precipitate and enrich umbilical cord mesenchymal stem cells. On the other hand, it can cooperate with the magnetism bands that are staggeredly distributed in the extraction device. In different processing parts, the magnetism bands are alternately distributed on both sides of the bottom of the chamber, so that the chamber can be alternately subjected to magnetic forces in different directions, thereby more fully binding or separating magnetic particles and nucleic acids. By reciprocally transporting the extraction part among different processing parts, rapid, effective, and automated nucleic acid extraction and purification processing of umbilical cord mesenchymal stem cells can be achieved. Description of the Drawings

[0005] Figure 1 It is a cross-sectional view and a top view of the extraction chamber part in the nucleic acid extraction device of the present utility model;

[0006] Figure 2 It is a side view and a top view of each processing part in the nucleic acid extraction device of the present utility model. Detailed Embodiment

[0007] The following will cooperate with embodiments to detail the implementation manner of the present utility model, so as to fully understand the implementation process of how to apply technical means to solve technical problems and achieve technical effects and implement accordingly.

[0008] The device of the present utility model is used for nucleic acid extraction and purification of mesenchymal stem cells. As shown in the attached Figure 1 figures, it includes a chamber 1 for accommodating mesenchymal stem cell samples. Inside the chamber 1, mesenchymal stem cell samples to be detected, magnetic particles, and liquid reagents are introduced. The chamber 1 gradually narrows in the width level (X-axis direction) from top to bottom, while the length of the chamber remains unchanged from top to bottom (Y-axis direction). The bottom 2 of the chamber has a cross-sectional length that is the same as that of the upper part of the chamber but the width is smaller. Multiple semi-circular grooves 3 parallel to the length direction of the chamber 1 are provided on the bottom 2. The radius of the semi-circular grooves 3 is small enough to generate capillary action, allowing the liquid to be evenly distributed or move along the axis of the semi-circular grooves 3. Multiple chambers 1 for accommodating mesenchymal stem cell samples are combined in an array to jointly form an extraction part 4. The extraction part 4 is arranged to be able to be transported and move between different processing modules.

[0009] As shown in the attached Figure 2As shown, the extraction unit 4 moves in a conveying manner along the X-axis direction between the vibration unit 5, the first magnetic field unit 6, the second magnetic field unit 7, and the temperature control unit 8. Magnetic force belts 9 are distributed at different positions in the first magnetic field unit 6 and the second magnetic field unit 7. When the extraction unit 4 is conveyed along the X-axis to the first magnetic field unit 6 or the second magnetic field unit 7, the corresponding magnetic force belts 9 are located at both ends of the bottom of the chamber 1 in the extraction unit 4. Since the magnetic force belts 9 in the first magnetic field unit 6 and the second magnetic field unit 7 are staggeredly distributed, when the extraction unit 4 is located in the first magnetic field unit 6 and the second magnetic field unit, the directions of the magnetic field attraction forces received by the bottom of the chamber 1 in the extraction unit 4 are opposite.

[0010] The process of nucleic acid extraction from mesenchymal stem cells by the device of the present utility model is as follows: The mesenchymal stem cell sample, the extraction reagent, and the magnetic particles are put into the multiple chambers 1 of the extraction unit 4. Since the width of the chamber 1 narrows from top to bottom, it is more conducive to the deposition and enrichment of the mesenchymal stem cell sample at the bottom. The extraction unit 4 is conveyed to the vibration unit 5 in a linear conveying manner, and vibration is generated at this position to ensure sufficient contact and reaction between the mesenchymal stem cell sample, the extraction reagent, and the magnetic particles. In addition, the vibration can cause the magnetic particles to detach from the side wall of the chamber 1 and re-suspend. When the extraction unit 4 is conveyed from the vibration unit to the first magnetic field unit 6 along the X-axis conveying direction, due to the distributed magnetic force belts 9, the magnetic particles in the extraction chamber 1 of the extraction unit 4 will displace in a direction perpendicular to X, that is, along the length direction of the extraction chamber 1. Therefore, the magnetic particles migrate to one side of the chamber 1. When the extraction unit 4 continues to move and enters the second magnetic field unit 7, since the magnetic force belts 9 in the second magnetic field unit 7 are staggeredly distributed, the magnetic particles in the extraction chamber 1 move from one side to the other side. Thus, the first and second magnetic field units can be selectively set to reciprocate, which increases the chance of magnetic particles capturing nucleic acids. On the other hand, during the inhibitor elution process, the efficiency of the separation of magnetic particles from nucleic acids is also increased. When temperature control is required during the nucleic acid extraction process, the extraction unit 4 can be selectively conveyed to the temperature control unit 8. Finally, after adding the inhibitor, the magnetic particles and nucleic acids are eluted. The extraction unit 4 can be controlled to be conveyed to the first and second magnetic field units. Thus, the magnetic particles are concentrated on one side of the chamber 1. By inserting an extraction tube into the side of the chamber 1 where there are no magnetic particles, highly efficient nucleic acid extraction can be achieved. The extraction unit 4 can also be selectively reciprocally conveyed in the first and second magnetic field units, so as to more fully separate the magnetic particles and nucleic acids and achieve better nucleic acid extraction.

[0011] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A nucleic acid purification device for umbilical cord mesenchymal stem cells, characterized in that: The device is provided with a chamber (1) for accommodating and purifying umbilical cord mesenchymal stem cells, the bottom of the chamber (1) is provided with a plurality of semicircular grooves (3) in parallel along its length direction, the plurality of chambers (1) are combined in an array to form an extraction section (4), the extraction section (4) is arranged to be movable between the plurality of processing sections, the device is further provided with a vibration section (5), a first magnetic field section (6), a second magnetic field section (7) and a temperature control section (8), a magnetic belt (9) is staggeredly distributed between the first magnetic field section (6) and the second magnetic field section (7), the magnetic belt (9) is arranged so that when the extraction section (4) is located between the first magnetic field section (6) and the second magnetic field section (7), the directions of magnetic field attraction received by the bottom of the chamber (1) in the extraction section (4) are opposite.

2. The device according to claim 1, characterized in that The chamber (1) gradually narrows in width from top to bottom, while remaining constant in length.

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