Bacterial drug sensitive test plate with scales
By designing a scaled bacterial drug sensitivity test plate, the size of the antibacterial circle is directly read, which solves the problems of complex operation, time-consuming and biohazard risk in the prior art, and achieves efficient, accurate and safe drug sensitivity test results.
Patent Information
- Application Number
- CN202421648316.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-12
- Publication Date
- 2025-06-10
- Estimated Expiration
- 2034-07-12
AI Technical Summary
The existing bacterial drug sensitivity test methods require the use of external measurement tools, which are complex and time-consuming, and have biohazard risks and measurement errors, which affect the accuracy of experimental results.
Design a scaled bacterial drug sensitivity test dish. Set a scale line on the body of the dish and the lid of the dish. The size of the antibacterial circle is directly read through the scale line, reducing dependence on external measurement tools, and design a scale ruler on the top of the inner side of the dish cover to avoid opening the lid to measure and reduce biosafety risks.
It realizes intuitive measurement of the antibacterial circle size without external measurement tools, improves work efficiency, reduces measurement errors and biosafety risks, and enhances the accuracy and safety of experimental results.
Smart Images
Figure CN222961417U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of medical equipment, and particularly relates to a bacteriological susceptibility test petri dish with scales. Background Technique
[0002] To test the sensitivity of bacteria to antibacterial compounds, simply referred to as susceptibility test, is the primary method for guiding the rational selection of antibacterial drugs in current medical laboratories. The disk diffusion method is the most common method for bacteriological susceptibility testing. According to the national health industry standard WS / T 639-2018 "Technical Requirements for Antimicrobial Susceptibility Testing", when measuring by the disk diffusion method, the petri dish should be placed against a black background, and the diameter (including the disk) of the area without obvious bacterial growth should be measured from the back of the petri dish using a vernier caliper or a ruler by means of reflected light. And if blood is added to the culture medium, the lid of the dish must be opened, and the diameter of the inhibition zone should be measured from the front by means of reflected light. The size of the inhibition zone reflects the sensitivity of the test bacteria to the antibacterial drug. Therefore, correctly and standardly measuring the size of the inhibition zone is a key step in the disk diffusion method susceptibility test. In the above operations, laboratory personnel need to measure each drug inhibition zone one by one with the help of measuring tools, resulting in a large workload; the correct use and fixation of external measuring tools have a great impact on the measurement results and require relatively high skills for laboratory personnel; opening the lid of the dish will expose the operator to a certain risk of biological hazards, which is not conducive to protecting the operator; and in daily work, some susceptibility test petri dishes are prone to fogging on the lid under room temperature conditions after being taken out of the incubator, affecting the routine measurement of laboratory personnel. Therefore, there is an urgent need in medical laboratories for a bacteriological susceptibility test petri dish that can measure directly, accommodate different susceptibility test requirements, improve the work efficiency of staff, and protect the operator.
[0003] In view of this, the utility model proposes a bacteriological susceptibility test petri dish with scales. Content of the Utility Model
[0004] The purpose of the utility model is to provide a bacteriological susceptibility test petri dish with scales, which can measure directly, accommodate different susceptibility test requirements, improve the work efficiency of staff, and protect the operator.
[0005] To achieve the above object, the present utility model provides the following technical solution: a bacteriological susceptibility test petri dish with scales, including a matching test petri dish body and a petri dish cover. A plurality of identification areas are provided at the fixed part of the test petri dish body and the petri dish cover. The identification areas arranged up and down are convenient for operators to identify through different marking strategies, so as to clarify the current detection scenario. And a number of scale lines are provided through the center of the circle corresponding to the part of the test petri dish body and the petri dish cover for the experimental dish plate; the test petri dish body is provided with scale lines along the center of the circle, and the position for pasting the drug sensitivity test paper is directly determined by relying on the scale axis; all the fixing rings on the test petri dish body are in the same reference system to calculate the inhibition zone; the scale lines provided on the petri dish cover can arbitrarily adjust the position of the petri dish cover as the petri dish cover rotates, so as to more clearly detect the inhibition zone. And under the marking effect of the identification area on the test petri dish body and the petri dish cover, the optical error can be estimated, and then the position of the petri dish cover is adjusted. When measuring data through the petri dish cover, the optical error can be appropriately adjusted to reduce the measurement error.
[0006] A plurality of fixing rings for fixing the drug sensitivity test paper are provided on the test petri dish body corresponding to the scale lines, and the fixing rings are evenly distributed on the test petri dish body; a plurality of layers of position dividing lines are provided on the center corresponding area of the center of the test petri dish body. The purpose of the multi-layer dividing lines provided in the center area is mainly to fix the center position. Based on the marking of the center position, the position of the scale line is clearer, and the position dividing line can reduce the spread of the inhibition zone to the center position. However, in actual application, the fixing ring for placing the drug sensitivity test piece is relatively far from the center position to avoid the cross influence of the inhibition zone at the center position. Therefore, in daily monitoring, the role of the position dividing line is not obvious. But when making full use of the culture dish, the cross influence of the inhibition zone can be effectively reduced through the position dividing line.
[0007] As a preferred technical solution of the present utility model, the axes provided on the identification area coincide, so that the corresponding scale lines on the test petri dish body and the petri dish cover are in the same dimensional space, and the scale line size where the fixing ring is located is used as a reference for marking the scale line size corresponding to the petri dish cover.
[0008] As a preferred technical solution of the present utility model, hollow buckles are provided on the petri dish walls corresponding to the test petri dish body and the petri dish cover in the identification area, and the distance between the two buckles is the activity range of the petri dish cover on the test petri dish body.
[0009] As a preferred technical solution of the present utility model, a hollow layer is provided in the dish plate part of the test petri dish body and the petri dish cover, and the hollow layer is secondarily injection-molded into the test petri dish body and the petri dish cover through an injection mold.
[0010] As a preferred technical solution of the present utility model, the hollow layer is made of a medical curly and foldable flexible plate; scale lines are arranged inside the hollow layer, and the scale lines include an auxiliary axis line inside the dish and scale lines at the bottom of the dish. A fixing circle is marked on the auxiliary axis line inside the dish, and the sticking position of the drug sensitivity test paper is determined by relying on the auxiliary axis line inside the dish; the scale lines at the bottom of the dish include an outer protective layer, and the range of the inhibition zone is measured by relying on the scale lines at the bottom of the dish. The wear of the scale lines can be reduced by setting the outer protective layer. Here, the functions of the scale lines are distinguished according to their different positions. Of course, the two functions can also be included in the scale lines arranged inside the hollow layer, reducing the use of the scale lines and the influence caused by the non-correspondence before and after marking.
[0011] As a preferred technical solution of the present utility model, an anti-fog coating is provided on the surface of the test petri dish body and the dish cover, so that it does not fog up after being taken out of the incubator. When measuring a blood-containing culture medium, no external tool is required, and the anti-fog coating on the dish cover prevents the dish cover from fogging up, enabling a clearer view of the antibacterial situation.
[0012] As a preferred technical solution of the present utility model, positioning points are arranged inside the fixing circle, and inoculation or sticking is directly completed by using the positioning points inside the dish, and then the sticking position of the drug sensitivity test paper is determined by relying on the scale lines, so as to determine the range of the inhibition zone to be measured.
[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:
[0014] The present utility model provides a method for quickly and standardizing the sticking of drug sensitivity test papers. Scale lines are designed at the inner bottom of the petri dish, and the sticking position of the drug papers can be located and confirmed according to the scale lines. When measuring the inhibition zone subsequently, direct reading can be carried out without the aid of external measuring tools, reducing the measurement difficulty and workload. An anti-fog coating for preventing the dish cover from fogging and adhering is provided on the culture dish, making it more convenient for the operator to read the inhibition zone and eliminating the need for special tools.
[0015] In addition, a scale is designed at the inner top of the dish cover. When performing special drug sensitivity tests such as blood-containing culture media, there is no need to open the dish cover for measurement, effectively preventing the potential risk of biological safety exposure for operators and being safer. This product is on the dish cover. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 is an exploded view of the upper and lower structures of the bacteria drug sensitivity test petri dish with scale of the present utility model;
[0017] Figure 2 is an unfolded view of the surface structure of the test petri dish body or the dish cover of the present utility model;
[0018] Figure 3 is a cross-sectional view of the internal structure of the test petri dish body or the dish cover of the present utility model.
[0019] In the figure: 1. Test petri dish body; 2. Petri dish cover; 3. Scale line; 4. Fixed ring; 5. Identification area; 6. Center area; 7. Hollow layer. Specific implementation mode
[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.
[0021] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "vertical", "upper", "lower", "horizontal", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present utility model.
[0022] In the description of the present utility model, it should also be noted that unless otherwise clearly specified and limited, the terms "set", "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.
[0023] The most commonly used drug susceptibility test petri dishes in current medical laboratories have a diameter of 100 mm, corresponding to an inner diameter of 90 mm in the dish; or a diameter of 150 mm, corresponding to an inner diameter of 140 mm in the dish. Referring to the relevant guidelines of the Clinical and Laboratory Standards Institute (CLSI) in the United States, a 100-mm-diameter petri dish can have at most 6 drug susceptibility test paper pieces pasted on it, and can be designed with 3 scale lines passing through evenly and crossing each other at an angle of 60° to mark the pasting positions of 6 drug susceptibility test paper pieces; a 150-mm-diameter petri dish can have at most 12 drug susceptibility test paper pieces pasted on it, and can be designed with 6 scale lines passing through evenly and crossing each other at an angle of 30° to mark the pasting positions of 12 drug susceptibility test paper pieces.
[0024] Embodiment 1
[0025] As Figure 1-2As shown in the figure, the utility model provides a technical solution: a bacteriological susceptibility test petri dish with scales. Taking a commonly used petri dish with a diameter of 100 mm as an example for illustration, other sized petri dishes can be designed with reference. It includes a test petri dish body 1 and a lid 2 that are transparently arranged. The test petri dish body 1 and the lid 2 are separately arranged, and a number of scale lines 3 are provided through the center of the circle on the test petri dish body 1 and the lid 2. For a petri dish with a diameter of 100 mm, at most 6 susceptibility test paper discs can be pasted. It can be designed with 3 scale lines 3 that are evenly crossed and have an included angle of 60° to mark the pasting positions of 6 susceptibility test paper discs; for a 150 mm diameter petri dish, at most 12 susceptibility test paper discs can be pasted. It can be designed with 6 scale lines that are evenly crossed and have an included angle of 30° to mark the pasting positions of 12 susceptibility test paper discs. Based on the scale lines 3, operators can conveniently find the correct positions for pasting the paper discs, ensuring the effectiveness of the susceptibility test results.
[0026] The prior art also continuously adds scale lines 3 to the susceptibility test petri dish to mark the positions and sizes of the susceptibility test paper discs. In practical applications, if only the scale axis is designed at the inner bottom of the petri dish, it is very difficult to play the role of identifying the susceptibility test paper discs. Therefore, in order to facilitate operators to fix the drug paper discs along the axis and effectively avoid the situation of non-standard pasting of the drug paper discs, a plurality of fixing rings 4 for fixing the susceptibility test paper discs are provided along the corresponding scale lines 3 at the bottom of the test petri dish body 1. The fixing rings 4 are evenly distributed on the test petri dish body 1. In this way, when the inhibition zones are generated by the susceptibility test paper discs in the experiment, the measurement of the inhibition zones generated after pasting the paper discs along the scale lines 3 at the bottom of the test petri dish body 1 no longer requires external measuring tools, and operators can quickly complete the calculation of the diameter of the inhibition zone.
[0027] The setting of the fixing rings 4 enables operators to paste the susceptibility test paper discs in a standardized manner, making their operations more rapid. At the same time, scale lines 3 are designed at the inner bottom of the test petri dish body 1. During actual operation, operators can position and confirm the pasting positions of the drug paper discs according to the axis corresponding to the scale lines 3, effectively avoiding the situation of non-standard pasting of the drug paper discs and ensuring the effectiveness of the susceptibility test results.
[0028] Among them: the scale lines 3 are short line markings with millimeter intervals, and long line markings are set at 5 mm or 10 mm during scale setting. The fixing rings 4 are marked with small oval circles to facilitate operators to count the diameters of the inhibition zones; the fixing rings 4 are circles with a similar diameter of 1 mm. When operators count the diameters of the inhibition zones, they can quickly obtain the number of fixing rings 4 on the axis of the scale lines 3, which is convenient for operators to quickly complete the distance positioning.
[0029] After the drug susceptibility test paper is pasted, it is usually incubated overnight and the inhibition zone produced by the drug susceptibility test paper is measured using scale line 3 the next day. Therefore, although the pasting of the drug susceptibility test paper and the use of the scale are combined, the drug susceptibility test paper mainly shows the inhibition zone of the drug, and scale line 3 is mainly used to measure the inhibition zone. Their functions are different, and the working environment will also change accordingly. During the measurement process, the scale line 3 set at the bottom of the test petri dish body 1 may not provide accurate readings due to the optical effect between the test petri dish body 1 and the dish cover 2. Moreover, there are errors in the data of the inhibition zone measurement itself, which seriously affects the reading of experimental data. In case of necessity, the operator may need to open the dish cover, which not only increases the chance of contact with bacteria during the drug susceptibility test but also brings unnecessary risk of bacterial invasion to the operator. Therefore, a scale 3 is designed on the inner top of the dish cover 2, so that there is no need to open the dish cover 2 during special drug susceptibility tests such as those with blood-containing culture medium, protecting the operator from the risk of exposure to the test bacteria.
[0030] Here is a technical difficulty: whether the scale lines 3 corresponding to the test petri dish body 1 and the dish cover 2 need to be maintained at a corresponding level, and whether the data read from the scale lines 3 at different positions need to be compared. In view of this, an identification area 5 is correspondingly arranged at the end of the scale line 3 far from the center of the circle. When the identification areas 5 set on the test petri dish body 1 and the dish cover 2 are aligned and fixed, it is ensured that the data read from the scale lines 3 on the test petri dish body 1 and the dish cover 2 are in one dimension and have reference value. When the operator reads the inhibition zone, they can directly read the data without the need for external measuring tools, reducing the measurement difficulty and workload. Even when measuring the inhibition zone of special drug tests, there is no need to open the dish cover, which is safer and effectively prevents the potential risk of biosafety exposure for the operator.
[0031] In addition, a 0.1% Pluronic F-68 non-ionic surfactant coating is added to the inner sides of the test petri dish body 1 and the dish cover 2, enabling the test petri dish body 1 and the dish cover 2 to effectively prevent fogging and adhesion, facilitating the operator's observation and reading.
[0032] The center area 6 represents the center position of the test petri dish body 1. In a few experiments, operations may be required at the center position, such as inoculating bacteria at the center to measure their motility; or pasting paper at the center to test the synergistic or inhibitory effects with other various drugs. Therefore, no special settings are generally made for the center area 6 on the test petri dish body 1. However, in this embodiment, in order to enable the test petri dish body 1 to better cope with various experimental scenarios, multiple layers of position dividing lines are set on the center area 6, along with scale lines starting from the center position for each layer of position dividing lines.
[0033] Embodiment 2
[0034] Such asFigure 3 As shown, the parts not detailed in the present invention are as shown in Embodiment 1. This embodiment extends the setting of the scale line 3. In practical applications, if the scale line 3 is set inside the petri dish body 1 and the lid 2, near the end of the drug sensitivity test, after the drug sensitivity test is completed, frequent cleaning will cause the scale line 3 to fade, so that the technical effects described in Embodiment 1 cannot be achieved, or the scale line 3 will shift after fading during cleaning, or misunderstandings caused by defects of the operator will all lead to deviations in the collection of experimental data, and such deviations are also inevitable. Therefore:
[0035] A hollow layer 7 is provided in the middle of the petri dish body 1 and the lid 2. The hollow layer 7 is internally provided with a scale line 3 and a fixing ring 4, that is, the scale line 3 and the fixing ring 4 are processed by a mold. During the processing and production, during the initial mold injection molding process, the scale line 3 and the fixing ring 4 are provided at the top of the initial mold. After the preliminary injection molding is completed, the scale line 3 and the fixing ring 4 appear on the surfaces of the petri dish body 1 and the lid 2, and other colors are used for coloring, so that the colors of the scale line 3 and the fixing ring 4 are different from the transparent color of the petri dish body 1 and the lid 2. Subsequently, the petri dish body 1 and the lid 2 are injection molded again until an independent sealed structure is formed for the petri dish body 1 and the lid 2, presenting an integrally formed shape.
[0036] Embodiment 3
[0037] The parts not detailed in the present invention are as shown in Embodiment 1. In this embodiment, the bacterial drug sensitivity test petri dish in Embodiment 1 is used to conduct a drug sensitivity test, and specific implementation steps are provided in the drug sensitivity experiment:
[0038] Step A1: Weigh an appropriate amount of Mueller-Hinton agar powder according to the product manual, sterilize it at 103.4 Kpa and 121.3 °C for 15 min by high-pressure steam, and then use the test petri dish body 1 in Embodiment 1 to prepare an MHA agar plate for use;
[0039] Where Mueller-Hinton agar refers to Mueller-Hinton Agar, and MHA is a solid medium commonly used in antimicrobial susceptibility testing (AST).
[0040] Step A2: According to the requirements in the health industry standard WS / T639-2018, inoculate the pre-prepared test bacterial suspension onto the prepared MHA agar plate for use;
[0041] Step A3: Use sterile forceps to stick the antimicrobial agent paper along the scale line 3 at the bottom of the test petri dish body 1 onto the MHA agar plate inoculated with the test bacterial suspension, and place it in an incubator for incubation;
[0042] Step A4: Take out the drug susceptibility petri dish, place it against a black background, and directly read and record the size of the inhibition zone from the bottom of the bacterial drug susceptibility test petri dish according to the scale line. For the bacterial drug susceptibility test petri dish with blood or other reagents added to the culture medium, there is no need to open the petri dish lid 2. The petri dish lid 2 can be rotated so that the scale line 3 on the petri dish lid 2 passes through the midline of the drug susceptibility paper strip, and the diameter of the inhibition zone can be directly measured from the front.
[0043] The bacterial drug susceptibility test petri dish adopted in this embodiment can be opened for use by enterprise or individual users. Enterprise users can use the bacterial drug susceptibility test petri dish to mass-produce products with different culture medium contents for further promotion, and individual users can use the bacterial drug susceptibility test petri dish to make the required culture petri dishes by themselves. Specifically, different sizes can be designed according to requirements, and parameters such as internal scale markings and the volume of the culture dish can be adjusted with reference to the requirements of guidelines such as CLSI and the actual needs of customers.
[0044] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A graduated bacterial drug sensitivity test plate, characterized in that: The invention comprises a test plate body (1) and a plate cover (2) which are matched with each other, a plurality of marking areas (5) are arranged at the fixing position of the test plate body (1) and the plate cover (2), and a plurality of scale lines (3) are arranged along the center of the circle of the test plate body (1) and the plate cover (2) relative to the test plate. The test plate body (1) is provided with a plurality of fixing rings (4) for fixing drug sensitivity test strips on the scale lines (3), and the fixing rings (4) are evenly distributed on the test plate body (1); and a plurality of position separation lines are provided on a circle center area (6) corresponding to the circle center of the test plate body (1).
2. A bacterial drug sensitivity test plate with scale according to claim 1, characterized in that: The axes arranged on the marking area (5) coincide with each other, so that the corresponding scale lines (3) on the test dish body (1) and the dish cover (2) are in the same dimensional space, and the size of the scale line (3) where the fixing ring (4) is located is used as a reference for referring to the size mark of the scale line (3) corresponding to the dish cover (2).
3. A graduated bacterial drug sensitivity test plate according to claim 2, characterized in that: The marking area (5) is provided with hollow buckles on the dish walls corresponding to the test dish body (1) and the dish cover (2), and the distance between the two buckles is the movable range of the dish cover (2) on the test dish body (1).
4. A graduated bacterial drug sensitivity test plate according to claim 1, characterized in that: The test dish body (1) and the dish cover (2) are characterized by having a hollow layer (7) disposed on the dish plate portion, and the hollow layer (7) is secondary injection molded into the test dish body (1) and the dish cover (2) through an injection mold.
5. A graduated bacterial drug sensitivity test plate according to claim 4, characterized in that: The hollow layer (7) is made of a medical curled and folded flexible plate; a scale line (3) is built into the hollow layer (7), and a fixing ring (4) is marked on the scale line (3).
6. A graduated bacterial drug sensitivity test plate according to claim 1, characterized in that: Anti-fog coatings are provided on the surfaces of the test dish body (1) and the dish cover (2).
7. A graduated bacterial drug sensitivity test plate according to claim 1, characterized in that: The fixing ring (4) has a built-in positioning point.