Phytoplankton concentration and enrichment sampling device
By designing a concentrated enrichment sampling device for phytoplankton samples, the siphon effect is used to reduce the disturbance of the supernatant on the precipitate, the problem of loss of phytoplankton is solved and the reliability of the detection results is improved.
Patent Information
- Application Number
- CN202421875973.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-05
- Publication Date
- 2025-06-10
- Estimated Expiration
- 2034-08-05
AI Technical Summary
The prior art can easily lead to the loss of phytoplankton population during the enrichment process of phytoplankton samples, resulting in the sample not having the original environmental characteristics and the measured results are inaccurate.
A phytoplankton concentrated and enriched sampling device is designed, including a collection bag, a liquid extraction tube and a suction device. The supernatant liquid is discharged through siphon, reducing disturbance to the precipitate and preventing the phytoplankton from absorbing with the supernatant liquid.
It effectively reduces the loss of phytoplankton in the sample during the enrichment operation, ensures the integrity of the number of phytoplankton in the sample, and improves the reliability of the detection results.
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Figure CN222964948U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a sampling device, in particular to a phytoplankton concentration and enrichment sampling device. Background Art
[0002] In the detection of environmental samples, whether the sampling method is reasonable and effective is crucial to the test results. A sample with reasonable sampling should be fully representative, fully reflect the diversity characteristics of organisms in the current environment, and fully reflect the current ecological environment status. In HJ1216-2021-Determination of Phytoplankton in Water Quality-0.1ml Counting Frame-Microscopic Counting Method, the sampling points, frequency, location, and sampling work of environmental sample sampling are clearly stipulated. The sampling process is roughly to use the plankton net to collect qualitative samples in points and layers, preliminarily filter the samples, and transfer the sediment to the qualitative sampling bottle. When conducting quantitative analysis, the sample collection volume shall generally not be less than 500ml. The collected samples must also be diluted or concentrated according to the density of phytoplankton. The concentration operation of the sample is complicated. During the concentration operation, it is necessary to repeatedly remove the supernatant and take the sediment. During the removal of the supernatant and the sediment, it is very easy to cause a large number of phytoplankton in the sample to be lost, resulting in the sample not having the original characteristics of the environment, making the measured results inaccurate.
[0003] Therefore, a phytoplankton concentration and enrichment sampling device is provided, which can ensure the integrity of the number of phytoplankton in the sample during the concentration and enrichment process of the sample. Summary of the invention
[0004] The utility model aims to solve the deficiencies in the prior art and provide a phytoplankton concentration and enrichment sampling device which can ensure the integrity of the number of phytoplankton in a sample during the concentration and enrichment process of the sample.
[0005] The purpose of the utility model is achieved through the following technical solutions: a phytoplankton concentration and enrichment sampling device, comprising a collecting bag, a liquid extraction tube, and an aspirator, wherein an insertion port is provided on the collecting bag, a sampling valve is provided at the bottom of the collecting bag, and a through-stop is provided on the liquid extraction tube; the collecting bag is a soft bag;
[0006] One end of the liquid extraction tube is an insertion end and is used to be inserted into the upper clear liquid in the collection bag. The aspirator is used to be connected to the other end of the liquid extraction tube and to make the upper clear liquid flow out along the liquid extraction tube through negative pressure. The liquid extraction tube discharges the upper clear liquid from the collection bag through siphon action.
[0007] When the utility model is used to concentrate and enrich the sample, the collection bag containing the sample is left to stand for a period of time to allow it to precipitate, thereby stratifying the sample, wherein the lower layer of the sample is the precipitation layer, and the upper layer of the sample is the upper clear liquid. During the standing process, the insertion buckle on the collection bag should be closed (such as covering the insertion port with a lid); after the standing is completed, the insertion port on the collection bag is opened (the lid is removed), the insertion end of the liquid extraction tube is inserted into the upper clear liquid in the collection bag through the insertion port, and then the other end (outflow end) of the liquid extraction tube is lower than the insertion end, the stopper is opened, and the liquid extraction port is closed. An aspirator is connected to the outflow end, and the negative pressure generated by the aspirator causes the upper clear liquid to flow out along the liquid extraction tube. The aspirator is then removed, and then the upper clear liquid is continuously discharged outward through the liquid extraction tube under the action of siphon, and the discharged upper clear liquid can be collected by a container; until the upper clear liquid is discharged; after the upper clear liquid is removed, the liquid extraction tube is removed, and the cap on the sampling valve is opened to discharge the enriched sediment in the collection bag; then the inner wall of the collection bag is rinsed with the upper clear liquid, and the above-mentioned concentration and enrichment operation is repeated, and the number of repetitions is 1-3 times. When the utility model concentrates and enriches the sample, the upper clear liquid is discharged through the siphon effect of the liquid suction tube. Since the siphon effect has little disturbance to the upper clear liquid and little effect on the liquid fluctuation, when the upper clear liquid is sucked away, the sediment below is not easily sucked away along with the upper clear liquid, which greatly reduces the loss of phytoplankton in the sample during the concentration and enrichment operation, ensures the integrity of the phytoplankton quantity in the sample, and improves the reliability of the detection result.
[0008] Preferably, the liquid extraction tube comprises a siphon section and a hose section, one end of the siphon section is an insertion end, and the stopper is connected to the hose section.
[0009] Preferably, the stopper is a buckle, which includes an elastic buckle body, a first extrusion protrusion and a second extrusion protrusion are provided on the inner side of the buckle body, the first extrusion protrusion and the second extrusion protrusion are arranged correspondingly, a neck area is formed between the first extrusion protrusion and the second extrusion protrusion, through holes are respectively provided at both ends of the buckle body, and the hose section passes through the through holes in the neck area and at both ends of the buckle body at the same time; a first hook body is provided at one end of the buckle body, and a second hook body matching the first hook body is provided at the other end of the buckle body.
[0010] Preferably, the aspirator is a rubber ball, and the rubber ball is provided with a connection port for connecting a liquid extraction tube.
[0011] Preferably, a limiting ring is provided on the siphon section, and the diameter of the limiting ring is larger than the diameter of the insertion port.
[0012] Preferably, the siphon section on the liquid extraction tube is made of hard material.
[0013] Preferably, the insertion port is arranged at the upper end of the collecting bag.
[0014] Preferably, the buckle is made of plastic.
[0015] The beneficial effect of the utility model is that when the sample is concentrated and enriched, the upper clear liquid is discharged through the siphon effect of the liquid suction tube. Since the siphon effect has little disturbance to the upper clear liquid and little liquid fluctuation, when the upper clear liquid is sucked away, the sediment below is not easily sucked away along with the upper clear liquid, which greatly reduces the loss of phytoplankton in the sample during the concentration and enrichment operation, ensures the integrity of the phytoplankton quantity in the sample, and improves the reliability of the detection result. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 It is a structural schematic diagram of the utility model.
[0017] Figure 2 This is a schematic diagram of the clip being connected to the hose.
[0018] Figure 3 This is a schematic diagram of the buckle in the open state.
[0019] Figure 4 It is a schematic diagram when the buckle is in a closed state.
[0020] Figure 5 This is a schematic diagram of the siphon section on the suction tube being inserted into the collection bag.
[0021] Figure 6 This is a schematic diagram of connecting the hose section on the liquid extraction tube to the rubber ball.
[0022] Figure 7 It is a schematic diagram of extracting the upper clear liquid in the collection bag through the extraction tube.
[0023] Figure 8 Schematic diagram of the phytoplankton concentrate being poured out from the collection bag.
[0024] In the figure: 1, collecting bag, 2, insertion port, 3, liquid extraction tube, 31, siphon section, 32, hose section, 4, limiting ring, 5, buckle, 51, buckle body, 53, first hook body, 54, second hook body, 55, first extrusion protrusion, 56, second extrusion protrusion, 57, through hole, 6, rubber ball, 7, sampling valve. DETAILED DESCRIPTION
[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the utility model.
[0026] like Figures 1 to 8 As shown, a phytoplankton enrichment sampling device comprises a collecting bag 1, a liquid extraction tube 3, and an aspirator. The collecting bag 1 is provided with an insertion port 2, which is arranged at the upper end of the collecting bag 1. A sampling valve 7 is provided at the bottom of the collecting bag 1, and a stopper is provided on the liquid extraction tube 3.
[0027] One end of the suction tube 3 is an insertion end and is used to be inserted into the upper clear liquid in the collection bag 1. The aspirator is used to be connected to the other end of the suction tube 3 and to make the upper clear liquid flow out along the suction tube 3 through the negative pressure. The suction tube 3 discharges the upper clear liquid from the collection bag 1 through the siphon effect.
[0028] When the utility model is used to concentrate and enrich the sample, the collection bag 1 containing the sample is left to stand for a period of time to allow it to precipitate, thereby stratifying the sample, wherein the lower layer of the sample is the precipitation layer, and the upper layer of the sample is the upper clear liquid. During the standing process, the insertion buckle on the collection bag 1 should be closed (such as covering the insertion port 2 with a lid); after the standing is completed, the insertion port 2 on the collection bag 1 is opened (the lid is removed), the insertion end of the liquid extraction tube 3 is inserted into the upper clear liquid in the collection bag 1 through the insertion port 2, and then the other end (the outflow end) of the liquid extraction tube 3 is lower than the insertion end, the stopper is opened, and the liquid extraction tube 3 is sucked out of the collection bag 1. An aspirator is connected to the outflow end of the liquid outlet, and the negative pressure generated by the aspirator causes the upper clear liquid to flow out along the liquid extraction tube 3, and then the aspirator is removed. Then, the upper clear liquid is continuously discharged outward through the liquid extraction tube 3 under the action of siphon, and the discharged upper clear liquid can be collected by a container; until the upper clear liquid is discharged; after the upper clear liquid is removed, the liquid extraction tube 3 is removed, and the cap on the sampling valve 7 is opened to discharge the enriched sediment in the collection bag 1; then the inner wall of the collection bag 1 is rinsed with the upper clear liquid, and the above-mentioned concentration and enrichment operation is repeated, and the number of repetitions is 1-3 times.
[0029] The collection bag used in the utility model is a soft bag, the material of the soft bag is soft, the volume is small after compression, the weight is light, and it is easy to carry, and is particularly suitable for outdoor sampling operations.
[0030] The liquid extraction tube 3 comprises a siphon section 31 and a hose section 32 . One end of the siphon section 31 is an insertion end, and a stopper is connected to the hose section 32 .
[0031] A limiting ring 4 is provided on the siphon section 31 , and the diameter of the limiting ring 4 is larger than the diameter of the insertion port 2 . When the siphon section 31 is inserted into the collecting bag 1 , the limiting ring 4 abuts against the insertion port 2 and limits the siphon section 31 .
[0032] In some embodiments of the present application, the siphon section 31 on the liquid extraction tube 3 is made of a hard material, and the hard material may be plastic, metal, or the like.
[0033] Further, the through stopper is a buckle 5, which includes an elastic buckle body 51, a first extrusion protrusion 55 and a second extrusion protrusion 56 are provided on the inner side of the buckle body 51, the first extrusion protrusion 55 and the second extrusion protrusion 56 are provided correspondingly, a neck area is formed between the first extrusion protrusion 55 and the second extrusion protrusion 56, through holes 57 are provided at both ends of the buckle body 51, and the hose section 32 passes through the neck area and the through holes 57 at both ends of the buckle body 51; a first hook body 53 is provided at one end of the buckle body 51, and a second hook body 54 matched with the first hook body 53 is provided at the other end of the buckle body 51. In some embodiments of the present application, the buckle 5 is made of plastic.
[0034] When the first hook body 53 and the second hook body 54 are connected, the buckle body 51 forms a closed loop structure, the width of the neck region reaches the minimum, and the hose section 32 is squeezed by the first squeezing protrusions 55 and the second squeezing protrusions 56 on both sides and is in a blocked state; when the first hook body 53 and the second hook body 54 are unhooked, the middle part of the buckle body 51 is disconnected, and the width of the neck region reaches the maximum. The first squeezing protrusions 55 and the second squeezing protrusions 56 squeeze the hose section 32 less, and the hose section 32 is in a conducting state. The opening or interruption of the liquid extraction tube 3 is controlled in the above manner.
[0035] The suction device is a rubber ball 6, and a connection port for connecting to the liquid extraction tube 3 is provided on the rubber ball 6. When the upper layer of clear liquid is extracted by the suction device, the rubber ball 6 is first squeezed to discharge the air in the rubber ball 6, and then the connection port on the rubber ball 6 is connected to one end of the liquid extraction tube 3. After the stopper is opened, the rubber ball 6 is released. During the recovery process, negative pressure is generated inside the rubber ball 6, and under the action of the negative pressure, the upper layer of clear liquid flows along the liquid extraction tube 3 to the outer tube mouth of the liquid extraction tube 3, and then the rubber ball 6 is quickly removed, and the upper layer of clear liquid is continuously discharged outward under the action of siphon. If the negative pressure generated by squeezing the rubber ball 6 once is not enough to draw the upper clear liquid to the outer tube opening of the liquid extraction tube 3, then after the rubber ball 6 completes one suction, first close the stopper, then remove the rubber ball 6 and squeeze the rubber ball 6 again, then connect the rubber ball 6 to the liquid extraction tube 3 and open the stopper, and draw the upper clear liquid again through the rubber ball 6 until the upper clear liquid is drawn to the outer tube opening of the liquid extraction tube 3 and a siphon effect is established.
[0036] When the utility model concentrates and enriches the sample, the upper clear liquid is discharged through the siphon effect of the liquid suction tube 3. Since the siphon effect has little disturbance on the upper clear liquid and little liquid fluctuation, when the upper clear liquid is sucked away, the sediment below is not easily sucked away along with the upper clear liquid, which greatly reduces the loss of phytoplankton in the sample during the concentration and enrichment operation, ensures the integrity of the phytoplankton quantity in the sample, and improves the reliability of the detection result.
[0037] The present utility model is not limited to the above-mentioned optimal implementation mode. Anyone can derive other various forms of products under the inspiration of the present utility model. However, no matter what changes are made in the shape or structure, all those having the same or similar technical solutions as the present application fall within the protection scope of the present utility model.
Claims
1. A phytoplankton concentration and enrichment sampling device, characterized in that: It includes a collecting bag, a liquid extraction tube, and an aspirator. The collecting bag is provided with an insertion port, the bottom of the collecting bag is provided with a sampling valve, and the liquid extraction tube is provided with a stopper. The collecting bag is a soft bag. One end of the liquid extraction tube is an insertion end and is used to be inserted into the upper clear liquid in the collection bag. The aspirator is used to be connected to the other end of the liquid extraction tube and to make the upper clear liquid flow out along the liquid extraction tube through negative pressure. The liquid extraction tube discharges the upper clear liquid from the collection bag through siphon action.
2. A phytoplankton concentration and enrichment sampling device according to claim 1, characterized in that: The liquid extraction pipe comprises a siphon section and a hose section, one end of the siphon section is an insertion end, and the stopper is connected to the hose section.
3. A phytoplankton concentration and enrichment sampling device according to claim 2, characterized in that: The stopper is a buckle, which includes an elastic buckle body, a first extrusion protrusion and a second extrusion protrusion are provided on the inner side of the buckle body, the first extrusion protrusion and the second extrusion protrusion are arranged correspondingly, a neck area is formed between the first extrusion protrusion and the second extrusion protrusion, through holes are respectively provided at both ends of the buckle body, and the hose section passes through the through holes in the neck area and at both ends of the buckle body at the same time; a first hook body is provided at one end of the buckle body, and a second hook body matching the first hook body is provided at the other end of the buckle body.
4. The phytoplankton concentration and enrichment sampling device according to claim 1, characterized in that: The aspirator is a rubber ball, and a connecting port for connecting a liquid extraction tube is arranged on the rubber ball.
5. The phytoplankton concentration and enrichment sampling device according to claim 1, characterized in that: A limiting ring is arranged on the siphon section, and the diameter of the limiting ring is larger than the diameter of the insertion port.
6. A phytoplankton concentration and enrichment sampling device according to claim 5, characterized in that: The siphon section on the liquid extraction pipe is made of hard material.
7. The phytoplankton concentration and enrichment sampling device according to claim 1, characterized in that: The insertion port is arranged at the upper end of the collecting bag.
8. The phytoplankton concentration and enrichment sampling device according to claim 3, characterized in that: The buckle is made of plastic.