Auxiliary smear inoculating needle device
By designing an auxiliary smear inoculation needle device, using the rotation mechanism of the connecting rod and the box lid, combined with the capillary action of the water stone and the blocking effect of silk, the problems of cumbersome microbial morphology observation and difficulty in uniform distribution of bacteria in the prior art are solved, and efficient and uniform bacterial distribution and microscopic observation effects are achieved.
Patent Information
- Application Number
- CN202421860544.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-02
- Publication Date
- 2025-06-10
- Estimated Expiration
- 2034-08-02
AI Technical Summary
The existing microbial micromorphic observation operation methods are cumbersome, time-consuming, and it is difficult to achieve uniform distribution of bacteria, affecting the effects of staining and microscopy observation.
An auxiliary smear inoculation needle device is designed to utilize the rotation mechanism of the connecting rod and the box lid, combined with the capillary action of the water stone and the blocking action of silk, to achieve uniform distribution of bacteria on the slide, and through the reusable inoculation head, reduce cumbersome operation and cross-contamination.
The uniform distribution of bacteria is achieved, the operation process is simplified, the experimental time is shortened, the cross-contamination is avoided, and the quality and efficiency of microscopic observation is improved.
Smart Images
Figure CN222964979U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microorganism smear, and particularly relates to an auxiliary smear inoculation needle device. Background Art
[0002] The microscopic morphology observation of microorganisms is a necessary step in microorganism teaching and the identification of new bacterial strains. The first step in observing bacteria and molds under a microscope is to place the bacteria or molds on a glass slide. The traditional operation methods are as follows: 1. For bacteria: Drop a drop of physiological saline on the glass slide, pick up a small amount of bacteria under sterile conditions and mix them thoroughly with the physiological saline, then gently spread them out. The physiological saline naturally dries and volatilizes, and then it is fixed. 2. For molds: The commonly used method for observing the microscopic morphology of molds is the insert method. After inoculating molds under sterile conditions, insert a sterile insert obliquely 2 cm away from the mold, and take it out for observation after the bacteria grow on the cover glass.
[0003] However, the above operation methods are relatively cumbersome and have the following problems:
[0004] 1. The amount of bacteria picked is often large, the amount of physiological saline is small, the concentration of bacteria is large, and continuous mixing is required to fully mix the physiological saline and the bacteria, which takes a long time, is laborious, and is prone to forming bacterial aggregates, causing difficulties for the next step of staining and observation. Moreover, it takes a long time to wait for a drop of water to naturally dry and volatilize.
[0005] 2. The cleaning of the inserts: The inserts are thin and have some dirt on them. First, they are washed with water, then soaked in acid, boiled, washed with water, soaked in alkali, boiled, washed with water, washed with water, dried by absorption, individually wrapped with strip filter paper and sterilized for later use. The operation is cumbersome and time-consuming.
[0006] 3. The cultivation time of molds is too long, and the hyphae will cover the inserts and it is difficult to observe; if the cultivation time is too short, there are a small number of hyphae or a small number of spore heads (sporangia). Since the hyphae and spores grow on both the front and back of the inserts, it is difficult to clearly see multiple hyphae and spore heads (sporangia) at the same focal length.
[0007] Moreover, the first step of staining is specimen preparation (bacterial coating, mold insert). The evenness of the bacteria on the glass slide not only affects staining but also affects microscopic observation. Due to the existing bacterial coating and mold insert methods, which are cumbersome, time-consuming, prone to forming bacterial aggregates, and greatly affected by the growth time of the bacteria, the microscopic observation results are poor.
[0008] Therefore, it is necessary to provide an auxiliary smear inoculation needle device to solve the above problems. Content of the Utility Model
[0009] The utility model provides an auxiliary smear inoculation needle device to solve the problems raised in the above background art.
[0010] To solve the above technical problems, the technical solution of the present utility model is as follows:
[0011] An embodiment of the present utility model provides an auxiliary smear inoculation needle device, including: a box body, a first connecting rod and a second connecting rod are respectively rotatably connected to the upper surface of the box body, a first box cover is fixedly connected to the arc surface of the first connecting rod, and a second box cover is fixedly connected to the arc surface of the second connecting rod; a storage rack is fixedly connected to the upper surface of the first box cover, a housing is placed inside the storage rack, an inner ring one and an inner ring two are respectively fixedly connected to the inner wall of the housing, a magnesium alloy sleeve is clamped to the inner wall of one end of the housing, and a water-absorbing stone wrapped with silk cloth is clamped to the inner wall of the magnesium alloy sleeve; a coating rod is slidably connected to the inner wall of the housing, a retaining ring is fixedly connected to the arc surface of the coating rod, a spring is sleeved on the arc surface of the coating rod, and both ends of the spring are respectively fixedly connected to the inner ring two and the retaining ring.
[0012] Through the above technical solution, the first connecting rod and the second connecting rod facilitate the rotation of the first box cover and the second box cover. The coating rod can be inserted into the storage rack for storage. Due to the capillary action of the water-absorbing stone, water is absorbed, and the bacteria in the water are blocked by the silk and stay on the silk. When the water-absorbing stone is dotted on the glass slide, the bacteria are evenly distributed on the glass slide. After use, the inoculation head is pushed into the second cavity (box body 3) by pressing the pressing head, and after cleaning and sterilization, it can be reused.
[0013] Further, a rubber ring is fixedly connected to the inner wall of one end of the housing, and the size of the inner wall of the rubber ring is adapted to the size of the outer wall of the magnesium alloy sleeve.
[0014] Through the above technical solution, when the magnesium alloy sleeve is inserted into the housing, friction occurs between the rubber ring and the magnesium alloy sleeve, which improves the friction between the two, thereby reducing the situation of the magnesium alloy sleeve falling off.
[0015] Further, a pressing head is fixedly connected to one end of the coating rod, and an arc-shaped plate is fixedly connected to the arc surface of the housing.
[0016] Through the above technical solution, the pressing head facilitates the user to press the coating rod, and the arc-shaped plate facilitates the user to hold the housing.
[0017] Further, the inside of the box body is separated into a first cavity and a second cavity by a partition board. A coating head plate is clamped to the inner wall of the first cavity, and a plurality of grooves are formed on the surface of the coating head plate.
[0018] Through the above technical solution, the coating head plate can be used to place the water-absorbing stone wrapped with silk, and the second cavity is used to store the used inoculation head.
[0019] Further, two baffle plates are fixedly connected to the surface of the box body, and the two baffle plates are respectively located below the first connecting rod and the second connecting rod.
[0020] Through the above technical solution, the baffle is used to limit the rotation angles of the first lid and the second lid.
[0021] Furthermore, four clamping plates are fixedly connected to the surface of the box body, clamping holes are formed in the surfaces of the clamping plates, two fixing plates are fixedly connected to the surfaces of both the first lid and the second lid, inserting rods are fixedly connected to the surfaces of the fixing plates, and the inserting rods are clamped with the clamping holes on the surfaces of the clamping plates.
[0022] Through the above technical solution, when the first lid and the second lid are closed, the inserting rods on the fixing plates are inserted into the clamping holes on the clamping plates, preventing the box body from not being tightly closed.
[0023] Furthermore, a screw rod is threadedly connected to the surface of the storage rack, a clamping ring is rotatably connected to the arc surface of the screw rod, and the clamping ring is of a semi-circular ring structure.
[0024] Through the above technical solution, by rotating the screw rod, the clamping ring is driven to fix the coating rod.
[0025] The above solution of the present utility model has at least the following beneficial effects:
[0026] 1. In the present utility model, bacteria are evenly distributed on the glass slide, facilitating fixation and staining for observation.
[0027] 2. In the present utility model, the inoculation head belongs to anhydrous coating, saving the time for water droplet volatilization, thereby shortening the experimental operation time.
[0028] 3. In the present utility model, each water-absorbing stone is coated with only one type of bacteria, avoiding cross-contamination and at the same time reducing the cumbersome operation of mixing bacteria and water droplets (for molds, making insert slides).
[0029] 4. In the present utility model, the inoculation head can be reused or some parts can be replaced, such as the silk cloth, the water-absorbing stone or the magnesium-aluminum alloy shell, thereby saving costs.
[0030] 5. In the present utility model, the ultra-clean workbench has a small space, and this design is delicate, which can reduce the space utilization degree.
[0031] 6. In the present utility model, the water-absorbing stone can also be used for related biological experiments such as screening of hybrid strains. BRIEF DESCRIPTION OF THE DRAWINGS
[0032] Figure 1 is the overall structural schematic diagram of the present utility model;
[0033] Figure 2 is the structural schematic diagram of the present utility model from another perspective;
[0034] Figure 3 is the disassembled structural schematic diagram of the outer shell and the coating rod in the present utility model;
[0035] Figure 4 It is a schematic cross-sectional structural diagram of the outer box in the utility model;
[0036] Figure 5 This utility model Figure 1 An enlarged schematic diagram of point A.
[0037] Description of reference numerals:
[0038] 1. Box body; 2. Cavity 1; 3. Cavity 2; 4. Connecting rod 1; 5. Connecting rod 2; 6. Box cover 1; 7. Box cover 2; 8. Baffle; 9. Clamping plate; 10. Fixing plate; 11. Inserting rod; 12. Coating head plate; 13. Storage rack; 14. Screw; 15. Snap ring; 16. Shell; 17. Arc plate; 18. Coating rod; 19. Pressing head; 20. Baffle ring; 21. Inner ring 1; 22. Inner ring 2; 23. Spring; 24. Rubber ring; 25. Aluminum-magnesium alloy sleeve; 26. Water-supplying stone wrapped with silk cloth. DETAILED DESCRIPTION
[0039] The exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.
[0040] like Figures 1 to 5 As shown, an embodiment of the utility model provides an auxiliary smear inoculation needle device, comprising: a box body 1, the upper surface of the box body 1 is rotatably connected with a connecting rod 1 4 and a connecting rod 2 5, the arc surface of the connecting rod 1 4 is fixedly connected with a box cover 1 6, and the arc surface of the connecting rod 2 5 is fixedly connected with a box cover 2 7; the upper surface of the box cover 6 is fixedly connected with a storage rack 13, a shell 16 is placed inside the storage rack 13, the inner wall of the shell 16 is respectively fixedly connected with an inner ring 1 21 and an inner ring 2 22, an inner wall of one end of the shell 16 is clamped with an aluminum-magnesium alloy sleeve 25, and the inner wall of the aluminum-magnesium alloy sleeve 25 is clamped with a water stone 26 wrapped with silk cloth; the inner wall of the shell 16 is slidably connected with a coating rod 18, the arc surface of the coating rod 18 is fixedly connected with a retaining ring 20, the arc surface sleeve of the coating rod 18 is provided with a spring 23, and the two ends of the spring 23 are respectively fixedly connected with the inner ring 22 and the retaining ring 20;
[0041] The interior of the outer shell 16 is a hollow structure. The inner ring one 21 and the inner ring two 22 prevent the coating rod 18 and the spring 23 from slipping out. When performing the coating work, wrap the water-absorbing stone 26 with a silk cloth, and use the aluminum-magnesium alloy sleeve 25 to clamp the water-absorbing stone 26 wrapped with silk, then take it out from the coating head plate 12. Subsequently, place the water-absorbing stone 26 wrapped with silk on the surface of the uniform bacterial suspension. Due to the capillary action of the water-absorbing stone 26, water is absorbed, and the bacteria in the water are blocked by the silk and stay on the silk. Then, dot the water-absorbing stone 26 on the glass slide, and the bacteria will be evenly distributed on the glass slide.
[0042] As Figure 3 and Figure 4 shown, the inner wall at one end of the outer shell 16 is fixedly connected with a rubber ring 24. The size of the inner wall of the rubber ring 24 is adapted to the size of the outer wall of the aluminum-magnesium alloy sleeve 25. One end of the coating rod 18 is fixedly connected with a pressing head 19, and the arc surface of the outer shell 16 is fixedly connected with an arc-shaped plate 17;
[0043] The friction between the rubber ring 24 and the aluminum-magnesium alloy sleeve 25 is relatively large. When the aluminum-magnesium alloy sleeve 25 is inserted into the outer shell 16 and contacts the rubber ring 24, the friction between the two can reduce the situation of the aluminum-magnesium alloy sleeve 25 detaching from the outer shell 16. The settings of the pressing head 19 and the arc-shaped plate 17 facilitate the user to perform the coating work better.
[0044] As Figure 1 shown, the interior of the box body 1 is separated into a cavity one 2 and a cavity two 3 by a partition. The inner wall of the cavity one 2 is clamped with a coating head plate 12, and several grooves are formed on the surface of the coating head plate 12;
[0045] The coating head plate 12 is used to store the upper inoculation head (aluminum-magnesium alloy sleeve 25, water-absorbing stone 26 wrapped with silk cloth), and the cavity two 3 is used to collect the used inoculation head (aluminum-magnesium alloy sleeve 25, water-absorbing stone 26 wrapped with silk cloth).
[0046] As Figure 1 and Figure 2 shown, two baffles 8 are fixedly connected to the surface of the box body 1. The two baffles 8 are respectively located below the connecting rod one 4 and the connecting rod two 5. Four clamping plates 9 are fixedly connected to the surface of the box body 1. A clamping hole is formed on the surface of the clamping plate 9. Two fixing plates 10 are fixedly connected to the surfaces of the box cover one 6 and the box cover two 7 respectively. An inserting rod 11 is fixedly connected to the surface of the fixing plate 10, and the inserting rod 11 is clamped with the clamping hole on the surface of the clamping plate 9;
[0047] The baffle 8 is used to limit the rotation angle of the box cover one 6 and the box cover two 7. When the box cover one 6 and the box cover two 7 are closed, the inserting rod 11 on the fixing plate 10 is inserted into the clamping hole on the clamping plate 9 to prevent the box body 1 from not closing tightly.
[0048] As Figure 5As shown, a screw rod 14 is threadedly connected to the surface of the storage rack 13, and a clamping ring 15 is rotatably connected to the arc surface of the screw rod 14. The clamping ring 15 is a semi-circular ring structure;
[0049] When the coating work is completed, the outer shell 16 and the coating rod 18 are placed in the storage rack 13. The clamping ring 15 is rotated so that the notch of the clamping ring 15 is stuck on the coating rod 18. As the screw rod 14 rotates, the clamping ring 15 gradually abuts against the end face of the outer shell 16, thereby fixing the outer shell 16 and facilitating the storage and carrying of the coating rod 18.
[0050] In the embodiment of the present utility model (working principle), first, the inoculation head (magnesium alloy sleeve 25, water stone 26 wrapped with silk cloth) can be stored in the groove on the coating head plate 12. Subsequently, through the frictional force between the rubber ring 24 and the magnesium alloy sleeve 25, the magnesium alloy sleeve 25 is stuck inside one end of the outer shell 16. Then, the inoculation head (magnesium alloy sleeve 25, water stone 26 wrapped with silk cloth) on the coating head plate 12 in the box body 1 is taken out by the magnesium alloy sleeve 25. The inoculation head (magnesium alloy sleeve 25, water stone 26 wrapped with silk cloth) is placed on the surface of the bacterial suspension. Due to the capillary action of the water stone 26, water is absorbed, and the bacteria in the water are blocked by the silk and stay on the silk. The inoculation head (magnesium alloy sleeve 25, water stone 26 wrapped with silk cloth) is dotted on a clean glass slide, and the bacteria are evenly distributed on the glass slide. At this time, the pressing head 19 on the coating rod 18 does not press, and the spring 23 is in a relaxed state. When the pressing head 19 is pressed, the used inoculation head (magnesium alloy sleeve 25, water stone 26 wrapped with silk cloth) can be driven into the cavity two 3. At this time, the spring 23 is in a compressed state. When the pressing head 19 is not under pressure, the spring 23 automatically resets, thereby driving the entire coating rod 18 to form an upward linear movement;
[0051] When it is necessary to open the first box cover 6 and the second box cover 7, the rear baffle 8 plays a limiting role to prevent the first box cover 6 and the second box cover 7 from rotating too much. When it is necessary to close, the front inserting rod 11 and the card hole on the card plate 9 form a closure to prevent the box body 1 from not closing tightly.
[0052] The above is the preferred embodiment of the present utility model. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the principle described in the present utility model, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present utility model.
Claims
1. An auxiliary smear inoculation needle device, characterized in that: include: A box body (1), wherein the upper surface of the box body (1) is rotatably connected to a connecting rod 1 (4) and a connecting rod 2 (5), the arc surface of the connecting rod 1 (4) is fixedly connected to a box cover 1 (6), and the arc surface of the connecting rod 2 (5) is fixedly connected to a box cover 2 (7); The upper surface of the box cover 1 (6) is fixedly connected to a storage rack (13), an outer shell (16) is placed inside the storage rack (13), an inner ring 1 (21) and an inner ring 2 (22) are respectively fixedly connected to the inner wall of the outer shell (16), an aluminum-magnesium alloy sleeve (25) is clamped to the inner wall of one end of the outer shell (16), and a water-loading stone (26) wrapped with silk cloth is clamped to the inner wall of the aluminum-magnesium alloy sleeve (25); The inner wall of the outer shell (16) is slidably connected to a coating rod (18), the arc surface of the coating rod (18) is fixedly connected to a retaining ring (20), the arc surface of the coating rod (18) is sleeved with a spring (23), and the two ends of the spring (23) are respectively fixedly connected to the second inner ring (22) and the retaining ring (20).
2. The auxiliary smear inoculation needle device according to claim 1, characterized in that: A rubber ring (24) is fixedly connected to the inner wall of one end of the outer shell (16), and the size of the inner wall of the rubber ring (24) matches the size of the outer wall of the aluminum-magnesium alloy sleeve (25).
3. The auxiliary smear inoculation needle device according to claim 1, characterized in that: One end of the coating rod (18) is fixedly connected to a pressing head (19), and the arc surface of the housing (16) is fixedly connected to an arc plate (17).
4. The auxiliary smear inoculation needle device according to claim 1, characterized in that: The interior of the box body (1) is divided into cavity one (2) and cavity two (3) by a partition, the inner wall of cavity one (2) is clamped with a coating head plate (12), and the surface of the coating head plate (12) is provided with a plurality of grooves.
5. The auxiliary smear inoculation needle device according to claim 4, characterized in that: Two baffles (8) are fixedly connected to the surface of the box body (1), and the two baffles (8) are respectively located below the first connecting rod (4) and the second connecting rod (5).
6. The auxiliary smear inoculation needle device according to claim 5, characterized in that: Four clamping plates (9) are fixedly connected to the surface of the box body (1), and clamping holes are provided on the surfaces of the clamping plates (9). Two fixing plates (10) are fixedly connected to the surfaces of the box cover 1 (6) and the box cover 2 (7), and insertion rods (11) are fixedly connected to the surfaces of the fixing plates (10), and the insertion rods (11) are clamped with the clamping holes on the surfaces of the clamping plates (9).
7. The auxiliary smear inoculation needle device according to claim 1, characterized in that: The surface of the storage rack (13) is threadedly connected to a screw rod (14), and the arc surface of the screw rod (14) is rotatably connected to a clamping ring (15), and the clamping ring (15) is a semicircular ring structure.