Mouse perfusion platform

Abstract

The utility model relates to the technical field of medicine, and discloses a mouse perfusion platform which comprises a fixed table, a confluence plate, a collection box and an infusion module. The confluence plate is arranged below a fixing plate of the fixing table, an angle is formed between the confluence plate and the fixing plate, and the collecting box is arranged at the end, away from the fixing plate, of the confluence plate; a plurality of mounting holes are formed in the fixing plate, and the mounting holes are detachably connected with the fixing pieces; the infusion module comprises an infusion head, an infusion pump and a control pedal, the infusion head is connected with the infusion pump, the control pedal is used for controlling the infusion pump, a carrying table is arranged on the fixing table, and the infusion pump is arranged on the carrying table. And manpower is saved.

Description

Technical Field

[0001] The utility model relates to the field of medical technology, in particular to a mouse perfusion platform. Background Art

[0002] Histochemical analysis technology is an indispensable part of biomedical research, aiming to visually display the chemical substances in cells through specific staining methods, so as to identify and quantitatively evaluate their functions and positions. This process is crucial for revealing the metabolic activities, functional performances and morphological changes of organisms under normal and pathological conditions. Before these studies are carried out, tissue samples must be prepared through a series of pretreatment steps (such as collection, fixation, embedding, sectioning, etc.). The fixation step is particularly critical because it can effectively preserve the original morphology and chemical properties of cells, inhibit the self-digestion activity of enzymes, prevent tissue disintegration and corruption, thus ensuring the accuracy and reliability of the staining process. Selecting appropriate fixatives and fixation methods is crucial for maintaining the structural and functional integrity of specific tissues (such as the central nervous system and the liver). Among them, using paraformaldehyde as a fixative and delivering it to the target tissue quickly and evenly through the perfusion method has been proven to be able to efficiently preserve cell structure and enzyme activity.

[0003] Paraformaldehyde, a high molecular form of formaldehyde, is widely used in the fixation process of animal tissues due to its excellent fixation properties (such as strong penetration power, small tissue shrinkage effect, low cost). Although it is an effective fixative, paraformaldehyde has potential health hazards and poses a severe challenge to the safety of experimental personnel and the environment. Therefore, it is particularly important to develop a system that can maximize the recovery and treatment of paraformaldehyde during the fixation process.

[0004] Currently, the mouse perfusion fixation devices in laboratories are usually handmade, lacking an efficient operation, recovery and treatment system. This not only increases the health risks of experimental personnel, but also poses a potential hazard to the environment because the untreated fixative waste liquid is directly discharged into the sewer. In addition, the existing methods are complex to operate and usually require two people to cooperate, resulting in a time-consuming and inefficient process.

[0005] In summary, there is an urgent need for a mouse perfusion platform that can effectively fix and perfuse mice, centrally collect the perfused liquid medicine, and save manpower. Summary of the Utility Model

[0006] In view of the problems existing in the prior art regarding mouse perfusion fixation, a mouse perfusion platform is proposed that can effectively fix and perfuse mice, centrally collect the perfused liquid medicine, and save manpower.

[0007] To solve the above problems, the technical solution of the utility model is as follows:

[0008] A mouse perfusion platform, comprising a fixing table, a confluence plate, a collection box, and an infusion module; a confluence plate is provided under the fixing plate of the fixing table, the confluence plate has an angle relative to the fixing plate, and a collection box is provided at one end of the confluence plate away from the fixing plate; a plurality of mounting holes are provided on the fixing plate, and the mounting holes are detachably connected to fixing members; the infusion module includes an infusion head, an infusion pump, and a control pedal, the infusion head is connected to the infusion pump, the control pedal is used to control the infusion pump, a carrier table is provided on the fixing table, and the infusion pump is provided on the carrier table.

[0009] As a preferred technical solution, the fixing members include four-limb fixing members and a neck fixing member.

[0010] As a preferred technical solution, the four-limb fixing members include fixing bolts and fixing cards; the fixing cards are detachably connected to the fixing plate through the fixing bolts.

[0011] As a preferred technical solution, the neck fixing member includes a torsion bolt and a pulling rope; the pulling rope is connected to the torsion bolt.

[0012] As a preferred technical solution, the torsion bolt includes a winding groove and a torsion rod, the pulling rope is wound in the winding groove, and the torsion rod is used to drive the torsion bolt to rotate.

[0013] As a preferred technical solution, the torsion rod includes a telescopic rod and a positioning pin, the positioning pin is connected to the telescopic rod, and the positioning pin is adapted to the mounting hole.

[0014] As a preferred technical solution, the confluence plate is provided with tapered strips.

[0015] The beneficial effects of the present utility model:

[0016] 1. The mouse perfusion platform of the present utility model includes a fixing table, a confluence plate, a collection box, and an infusion module. Mounting holes are provided on the fixing plate of the fixing table, and a confluence plate is provided under the fixing plate. The confluence plate has an angle relative to the fixing plate, and a collection box is provided at one end of the confluence plate away from the fixing plate, so that the liquid medicine in the experiment can be concentrated and collected into the collection box through the mounting holes and the confluence plate for centralized treatment, and the mounting holes can provide modular selection for the fixation of the experimental object, increasing the flexibility of the experiment; the infusion module includes an infusion head, an infusion pump, and a control pedal, and the control pedal can control the perfusion of the liquid medicine by using the feet of the experimenter, saving labor costs.

[0017] 2. The mouse perfusion platform of the present utility model is provided with tapered strips on the confluence plate, and the tapered strips are opposite to the mounting holes, so that the liquid dripping from the mounting holes drops on the inclined surface of the tapered strips, effectively reducing the possibility of liquid medicine splashing. Description of the Drawings

[0018] Figure 1 This is a three-dimensional schematic diagram of a mouse perfusion platform of the present utility model;

[0019] Figure 2 This is a partially enlarged view of a mouse perfusion platform of the present utility model.

[0020] The reference numerals and components involved in the drawings are as follows:

[0021] 1. Fixed platform; 2. Confluence plate; 3. Collection box; 4. Fixing member; 11. Fixing plate; 21. Tapered strip; 41. Limb fixing member; 42. Neck fixing member; 51. Infusion head; 52. Infusion pump; 53. Control pedal; 111. Mounting hole; 421. Torsion bolt; 422. Pulling rope; 4211. Telescopic rod; 4212. Positioning pin. Detailed Embodiment

[0022] In order to make the above objects, features, and advantages of the present utility model more obvious and understandable, the following detailed description of the specific embodiments of the present utility model will be given with reference to the accompanying drawings of the specification.

[0023] Please refer to the attached Figure 1 and the attached Figure 2 , Figure 1 This is a three-dimensional schematic diagram of a mouse perfusion platform of the present utility model; Figure 2This is a partially enlarged view of a mouse perfusion platform of the present utility model. A mouse perfusion platform includes a fixed table 1, a confluence plate 2, a collection box 3, and an infusion module; the fixed table 1 includes a fixed plate 11 and four support columns for supporting it below; a number of mounting holes 111 are arranged in an array on the fixed plate 11. In this embodiment, the mounting holes 111 are bolt holes; below the fixed plate 11 is provided the confluence plate 2, and the confluence plate 2 is also arranged on the support columns, and one end of the confluence plate 2 is farther away from the fixed plate 11 than the other end, so that the confluence plate 2 is inclined relative to the horizontal plane, and the fixed table 1 is placed on the horizontal plane; three sides of the confluence plate 2 are provided with enclosures, and the other end relatively far away from the fixed plate 11 is connected to the collection box 3. The collection box 3 is a boxed structure with an open upper end. On the plate surface of the confluence plate 2 are provided a number of tapered strips 21, and the tapered strips 21 are arranged along the inclined direction of the confluence plate 2, and one conical angle side faces the mounting hole 111 above it; preferably, in order to facilitate the disassembly of the collection box 3, a crossbar is provided on the support columns, and the collection box 3 can be placed on the crossbar, and the pull-out disassembly and installation of the collection box 3 can be realized; the mounting holes 111 are used for detachably connecting with the fixing member 4. The fixing member 4 includes a limb fixing member 41 for fixing the limbs and a neck fixing member 42 for fixing the neck. The limb fixing member 41 includes a fixing bolt and a fixing card. The fixing bolt is a bolt structure, and the fixing card is an arch bridge structure with a raised middle. The two ends of the fixing card are fixed on the mounting hole 111 through the fixing bolt to achieve corresponding fixation. The neck fixing member 42 includes a torsion bolt 421 and a pulling rope 422. The torsion bolt 421 is provided with a winding groove and a torsion rod. One end of the pulling rope 422 is connected to the winding groove. The rotation of the torsion bolt 421 will cause the pulling rope 422 to rotate and wind along the winding groove, realizing the tightening and relaxation of the pulling rope 422. In order to enable the torsion bolt 421 to rotate smoothly, the torsion bolt 421 is a rod-shaped structure, and one end of it passes through the other end of the mounting hole 111 and is fixed to the rod-shaped structure through a nut, that is, the rotation axis of the torsion bolt 421 is located in the bolt teeth of the mounting hole 111. The torsion bolt 421 is provided with a torsion rod for convenient rotation, and the torsion rod includes a telescopic rod 4211 and a positioning pin 4212. One end of the telescopic rod 4211 is connected to the torsion bolt 421, and one end is connected to the positioning pin 4212. The positioning pin 4212 is adapted to the mounting hole 111. It should be understood that the positioning pin 4212 is also a rod-shaped structure without a bolt and can be directly inserted into the mounting hole 111;The infusion module includes an infusion head 51, an infusion pump 52, and a control pedal 53. The infusion head 51 is used to provide puncture injection. Preferably, a three-way valve is provided thereon to cope with the perfusion of different medicinal liquids. One end of the infusion head 51 is connected to the infusion pump 52. The infusion pump 52 contains corresponding medicinal liquids and can perform automatic injection through a micro pump. The infusion pump 52 may also be provided with a syringe barrel with a mechanical telescopic device, and its mechanical telescopic device may be a screw telescopic structure controlled by a stepping motor. Preferably, for the convenience of controlling the infusion module, the infusion pump 52 is connected to the control pedal 53, and the control pedal 53 can control the opening and closing of the infusion pump 52, which is equivalent to a switch for extending the circuit, and all are prior arts.;

[0024] The working principle of the present utility model:

[0025] First, prepare paraformaldehyde solution: Weigh 4 g of paraformaldehyde and dissolve it in 100 mL of 0.1 mol / L phosphate buffer solution. Stir and heat it on a magnetic stirrer, dropwise add 1 mol / L NaOH solution until it becomes clear, adjust the pH value to 7.4, filter it with qualitative filter paper to obtain a paraformaldehyde solution with a mass concentration of 4%, and store it in the dark at 4°C for standby. Prepare chloral hydrate solution: Weigh 4.3 g of chloral hydrate and dissolve it in 100 mL of 0.1 mol / L phosphate buffer solution, stir evenly to obtain a chloral hydrate solution with a mass concentration of 4.3%, and store it in the dark at 4°C for standby.

[0026] Second: Anesthesia; Use SPF-grade BALB / c mice with a body weight of 20 - 30 g as the collection group, regardless of gender. Take the chloral hydrate solution and perform intraperitoneal injection anesthesia on the mice at a dose of 0.01 mL / g body weight. After 2 - 3 minutes, pinch the hind limbs of the mice. If there is no reaction, it means the anesthesia level is appropriate. If there is a limb twitching reaction, add a little anesthetic to avoid muscle spasm caused by surgical pain compressing blood vessels and affecting the perfusion effect.

[0027] Third: Fixation and perfusion; Use the four-limb fixing parts 41 to spread and fix the four limbs of the mice on the fixing plate 11, and tie the pulling rope 422 to the teeth of the mice to fix the heads of the mice. The neck fixing parts 42 are respectively arranged on both sides of the heads of the mice. It should be understood that by rotating the torsion rod, the pulling of the pulling rope 422 is realized, thereby rotating the head position of the mice. The positioning pin 4212 cooperates with the telescopic rod 4211 to stretch, so that the positioning pin 4212 can be inserted into the mounting holes 111 in different directions, improving the freedom of rotation angle. After fixation, puncture and perfusion are performed on the mice.

[0028] Fourth: Sampling: Cut the skin of the mouse's head along the midline of the two ears, cut the cervical vertebrae behind the foramen magnum, separate and remove the posterior neck muscles, use the tip of a scalpel to cut open the skull along the cranial suture, taking care not to damage the brain. Starting from the foramen magnum with sharp forceps, gradually remove the cranial bones, taking care to clean the meninges on the surface of the brain and the broken bones on both sides. Use an ophthalmic scissors to cut the optic nerve on both sides and probe to the base of the skull, and the entire brain tissue can be removed. The successfully perfused and fixed brain tissue has considerable hardness and elasticity, is white, and has no blood color. Place the intact removed brain tissue in a centrifuge tube containing paraformaldehyde solution and continue to fix it at 4°C for 4 - 6 hours. Then replace the paraformaldehyde solution in the centrifuge tube with a 30% mass concentration of sucrose solution and dehydrate it at 4°C until the brain tissue sinks to the bottom of the centrifuge tube. Finally, put the dehydrated brain tissue into a cryogenic storage tube, quickly freeze it in liquid nitrogen for more than 20 seconds, and store it at -80°C.

[0029] Fifth, clean the fixing table 1. During the experiment and cleaning, the liquid medicine and other liquids drip down along the mounting hole 111 and flow along the confluence plate 2 into the collection tank.

[0030] It should be noted that: 1. The mouse perfusion platform of the present utility model includes a fixing table 1, a confluence plate 2, a collection box 3, and an infusion module. The fixing table 1 has a mounting hole 111 on its fixing plate 11, and the confluence plate 2 is provided under the fixing plate 11. The confluence plate 2 has an angle relative to the fixing plate 11. The collection box 3 is provided at one end of the confluence plate 2 away from the fixing plate 11, so that the liquid medicine during the experiment can be concentrated and collected into the collection box 3 through the mounting hole 111 and the confluence plate 2 for centralized treatment. And the mounting hole 111 can provide modular selection for the fixation of the experimental object, increasing the flexibility of the experiment; the infusion module includes an infusion head 51, an infusion pump 52, and a control pedal 53. The control pedal 53 can control the perfusion of the liquid medicine by the experimenter's foot, saving labor costs; on the confluence plate 2 of the mouse perfusion platform of the present utility model, there is also a tapered strip 21, and the tapered strip 21 is opposite to the mounting hole 111, so that the liquid dripping from the mounting hole 111 drips on the inclined surface of the tapered strip 21, effectively reducing the possibility of liquid medicine splashing.

[0031] The above is only the preferred embodiment of the present utility model. It should be pointed out that for those of ordinary skill in the art of this technology, without departing from the principle of the present utility model, several improvements and supplements can still be made, and these improvements and supplements should also be regarded as the protection scope of the present utility model.

Claims

1. A mouse perfusion platform, characterized in that: It includes a fixed platform, a convergence plate, a collection box, and an infusion module; the convergence plate is arranged under the fixed plate of the fixed platform, the convergence plate has an angle relative to the fixed plate, and the collection box is arranged at one end of the convergence plate away from the fixed plate; a plurality of mounting holes are arranged on the fixed plate, and the mounting holes are detachably connected to the fixing parts; the infusion module includes an infusion head, an infusion pump, and a control pedal, the infusion head is connected to the infusion pump, the control pedal is used to control the infusion pump, a carrier is arranged on the fixed platform, and the infusion pump is arranged on the carrier.

2. The mouse perfusion platform according to claim 1, characterized in that: The fixing parts include limb fixing parts and neck fixing parts.

3. The mouse perfusion platform according to claim 2, characterized in that: The limb fixing parts include a fixing bolt and a fixing card; the fixing card is detachably connected to the fixing plate via the fixing bolt.

4. The mouse perfusion platform according to claim 2, characterized in that: The neck fixing part comprises a torsion bolt and a pulling rope; the pulling rope is connected to the torsion bolt.

5. The mouse perfusion platform according to claim 4, characterized in that: The torsion bolt comprises a winding groove and a torsion rod, the pulling rope is wound in the winding groove, and the torsion rod is used for driving the torsion bolt to rotate.

6. The mouse perfusion platform according to claim 5, characterized in that: The torsion bar comprises a telescopic rod and a positioning pin, wherein the positioning pin is connected to the telescopic rod and is matched with the mounting hole.

7. The mouse perfusion platform according to claim 1, characterized in that: The busbar is provided with a tapered strip.

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