Auxiliary device for cell slide experiment
By designing an auxiliary device for cell crawler experiments including an annular pressure plate and sealing ring, the problem of uneven staining caused by dropping antibodies or dyes on cell crawlers is solved, and a more uniform staining effect and a clearer facial distinction between cell crawlers is achieved.
Patent Information
- Application Number
- CN202421668761.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-15
- Publication Date
- 2025-06-10
- Estimated Expiration
- 2034-07-15
AI Technical Summary
In the prior art, antibodies or dyes are easily leaked when added dropwise on cell crawlers, resulting in uneven staining.
Design a cell crawler experiment auxiliary device, including a base and upper cover, clamp the cell crawler through an annular pressure plate and sealing ring, limiting the flow area of the antibody or dye, thereby improving the uniformity of staining.
Through the above-mentioned device, antibodies or dyes can only flow in a defined area, significantly improving the uniformity of staining, and can significantly distinguish the front and back of cell crawlers, making it easier to label and group.
Smart Images
Figure CN222964984U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to experimental apparatus, in particular to an auxiliary device for a cell smear experiment. Background Art
[0002] The existing steps of a cell smear experiment include: 1) placing a sterile cell smear in a well plate, adding a cell suspension, culturing the cells after grouping experiments for subsequent immunohistochemical staining or other specific staining; 2) performing fixation penetration and other treatments on the cell smear that has adhered cells in the well plate; 3) cutting a sealing film slightly larger than the area of the smear, placing it on a wet box, dropping 100 μl / section of the primary antibody, taking out the smear with a hooked needle, and inverting and covering it on the primary antibody in sequence, incubating overnight at 4°C; 4) dropping 100 μl / section of the secondary antibody on the sealing film, taking out the smear with a hooked needle, and inverting and covering it on the secondary antibody in sequence, incubating the FITC-labeled secondary antibody in the dark at room temperature for 1 h; 5) putting the smear back into the well plate (with the side where the cells adhered facing up) for washing; 6) dropping DAPI dye or other dyes on the sealing film, taking out the smear with a hooked needle, and inverting and covering it on the dye in sequence, continuing to incubate for subsequent experiments, and finally inverting and mounting the smear on a glass slide. Directly dropping the antibody or dye on the cell smear easily leaks out, resulting in uneven staining. Summary of the Invention
[0003] The technical problem to be solved by the utility model is to provide an auxiliary device for a cell smear experiment, which solves the problem of uneven staining caused by the leakage of the antibody or dye dropped on the cell smear.
[0004] To solve the above technical problem, the technical solution of the utility model is: an auxiliary device for a cell smear experiment, including a base and an upper cover, one side of the upper cover is rotatably connected to the base, and the other side of the upper cover is fixedly connected to the base through a buckle; the base includes a supporting plate, and the supporting plate is provided with a hollow hole; the upper cover includes an annular pressing plate and a sealing ring provided on the bottom surface of the annular pressing plate. The principle of the utility model: first place the cell smear face up on the supporting plate of the base; then flip and close the upper cover and fix it to the base through the buckle. At this time, the cell smear is located between the upper cover and the base, and the annular pressing plate presses tightly on the top edge of the cell smear through the sealing ring; finally, drop the antibody or dye in the middle of the annular pressing plate. Under the action of the annular pressing plate and the sealing ring, the antibody or dye can only flow in a limited area, improving the uniformity of staining.
[0005] As an improvement, the outer diameter of the annular pressing plate is larger than the diameter of the cell smear, and the inner diameter of the annular pressing plate is smaller than the diameter of the cell smear.
[0006] As an improvement, the shape of the sealing ring is similar to the shape of the annular pressing plate.
[0007] As an improvement, two cross - arranged ribs are provided on the supporting plate. Limit blocks are provided at both ends of the ribs, and limit grooves are formed inside the limit blocks.
[0008] As an improvement, a marking area is provided on the annular pressing plate.
[0009] The beneficial effects brought by the present utility model compared with the prior art are as follows:
[0010] The upper cover and the base can clamp and fix the cell slide. Under the action of the annular pressing plate and the sealing ring, the antibody or dye can only flow in the limited area, improving the uniformity of staining; through the structural difference between the upper cover and the base, the front and back sides of the cell slide can be clearly distinguished; the upper cover is provided with a marking area, which is convenient for marking and grouping. Description of the Drawings
[0011] Figure 1 It is a schematic diagram of the open state of the device.
[0012] Figure 2 It is a schematic diagram of the closed state of the device. Detailed Embodiment
[0013] The present utility model will be further described below in conjunction with the drawings in the specification.
[0014] Such as Figure 1 、 2As shown in the figure, a cell smear experiment auxiliary device includes a base 2 and an upper cover 1. One side of the upper cover 1 is rotatably connected to the base 2 through a connecting piece 4, and the other side of the upper cover 1 is fixedly connected to the base 2 through a buckle 3. The base 2, the upper cover 1, the connecting piece 4 and the buckle 3 can be integrally injection-molded. The buckle 3 includes a buckling part provided on the upper cover 1 and a blocking part provided on the base 2. The buckle 3 is a prior art and will not be elaborated in this embodiment. The base 2 includes a circular supporting plate 24, the diameter of the supporting plate 24 is larger than the diameter of the cell smear 5, and the supporting plate 24 is provided with a hollow hole 21; two convex ribs 22 arranged in a cross shape are provided on the supporting plate 24. The two convex ribs 22 are perpendicular to each other and pass through the center of the supporting plate 24. Limiting blocks 23 are provided at both ends of the convex ribs 22. Limiting grooves are formed inside the four limiting blocks 23. The limiting grooves can limit the position of the cell smear 5. After the cell smear 5 is placed in the limiting groove, the top surface of the cell smear 5 is not lower than the top surface of the limiting block 23, so that the upper cover 1 can press the cell smear 5 tightly. The upper cover 1 includes an annular pressing plate 11 and a sealing ring 12 provided on the bottom surface of the annular pressing plate 11; the shape of the sealing ring 12 is similar to the shape of the annular pressing plate 11, and the sealing ring 12 is fixed on the annular pressing plate 11 by glue. The annular pressing plate 11 serves as the skeleton of the sealing ring 12, so that the sealing ring 12 can fit well with the cell smear 5; the outer diameter of the annular pressing plate 11 is larger than the diameter of the cell smear 5, and the inner diameter of the annular pressing plate 11 is smaller than the diameter of the cell smear 5; a marking area 13 is provided on the annular pressing plate 11. The marking area 13 can be an area protruding from one side of the annular pressing plate 11. The upper cover 1 is provided with a marking area, which is convenient for marking groups.
[0015] Principle of the present utility model: First, place the cell smear 5 face up on the supporting plate 24 of the base 2; then flip the upper cover 1 and close it, and fix it to the base 2 through the buckle. At this time, the cell smear 5 is located between the upper cover 1 and the base 2, and the annular pressing plate 11 presses tightly on the top edge of the cell smear 5 through the sealing ring 12; finally, drop the antibody or dye in the middle of the annular pressing plate 11. Under the action of the annular pressing plate 11 and the sealing ring 12, the antibody or dye can only flow in the limited area, improving the uniformity of staining.
Claims
1. A cell climbing experiment auxiliary device, characterized in that: It includes a base and an upper cover, one side of the upper cover is rotatably connected to the base, and the other side of the upper cover is fixedly connected to the base through a buckle; the base includes a supporting plate, and the supporting plate is provided with a hollow hole; the upper cover includes an annular pressure plate and a sealing ring arranged on the bottom surface of the annular pressure plate.
2. A cell climbing experiment auxiliary device according to claim 1, characterized in that: The outer diameter of the annular pressing plate is larger than the diameter of the cell climbing sheet, and the inner diameter of the annular pressing plate is smaller than the diameter of the cell climbing sheet.
3. A cell climbing experiment auxiliary device according to claim 1, characterized in that: The shape of the sealing ring is similar to that of the annular pressure plate.
4. The cell climbing experiment auxiliary device according to claim 1, characterized in that: The support plate is provided with two cross-shaped convex ribs, both ends of the convex ribs are provided with limit blocks, and the inner sides of the limit blocks form limit grooves.
5. The cell climbing experiment auxiliary device according to claim 1, characterized in that: The annular pressing plate is provided with a marking area.